The document summarizes genetic characterization of foot-and-mouth disease viruses in Southeast Asia. It analyzed 95 clinical samples collected between 2018-2019 from Vietnam, Laos, and Cambodia. Through 3D qRT-PCR analysis, 40 samples were confirmed positive for foot-and-mouth disease virus. Phylogenetic analysis based on the VP1 gene found that viruses from Cambodia belonged to serotypes O and A, classified as O/ME-SA/Panasia and A/ASIA/SEA-97 genotypes. Viruses from Vietnam and Laos belonged to serotype O, classified as O/ME-SA/Panasia and O/SEA/Mya-98 genotypes. The study provides information on the genetic
Perspectives of predictive epidemiology and early warning systems for Rift Va...ILRI
Presentation by MO Nanyingi, GM Muchemi, SG Kiama, SM Thumbi and B Bett at the 47th annual scientific conference of the Kenya Veterinary Association held at Mombasa, Kenya, 24-27 April 2013.
Perspectives of predictive epidemiology and early warning systems for Rift Va...ILRI
Presentation by MO Nanyingi, GM Muchemi, SG Kiama, SM Thumbi and B Bett at the 47th annual scientific conference of the Kenya Veterinary Association held at Mombasa, Kenya, 24-27 April 2013.
Two main animal pathogenic subspecies of Mycobacterium avium are M. avium avium (Maa) and M. avium paratuberculosis (Map). Their pathogenicity is host-specifi c, Maa causing avian tuberculosis in poultry whereas Map commonly cross-infects to ruminant.Veterinary diagnosis of M. avium infections is microscopic examination of acid-fast bacilli or culture in Löwenstein-Jensen medium,which are time-consuming and low sensitivity. This present study aimed to apply real-time PCR coupled with High-Resolution Melting (HRM) analysis for differential detection of Maa in Thai domestic ducks. Specifi c primer targeting host-expression dependent (hed) region
was designed, PCR product of Maa were amplifi ed from duck’s tissue lesions whereas Map were amplifi ed from cow and deer. HRM real-time PCR was performed and analyzed. Different HRM patterns were showed and melting temperature were analyzed at 83.26 ± 0.12°C for Maa and 84.04 ± 0.09°C for Map. This technique can detect as few as 102 DNA copies and present high specifi city by negative amplifi cation of other pathogenic bacterial species. This technique is sensitive, specifi c, rapid and does not require fl uorescent probes or post-PCR electrophoresis. Our technique is a possible new tool for the detection of Maa and Map infection in tissue specimens.
Two main animal pathogenic subspecies of Mycobacterium avium are M. avium avium (Maa) and M. avium paratuberculosis (Map). Their pathogenicity is host-specific, Maa causing avian tuberculosis in poultry whereas Map commonly cross-infects to ruminant. Veterinary diagnosis of M. avium infections is microscopic examination of acid-fast bacilli or culture in Löwenstein-Jensen medium,which are time-consuming and low sensitivity. This present study aimed to apply real-time PCR coupled with High-Resolution Melting (HRM) analysis for differential detection of Maa in Thai domestic ducks. Specific primer targeting host-expression dependent (hed) region
was designed, PCR product of Maa were amplified from duck’s tissue lesions whereas Map were amplified from cow and deer. HRM real-time PCR was performed and analyzed. Different HRM patterns were showed and melting temperature were analyzed at 83.26 ± 0.12°C for Maa and 84.04 ± 0.09°C for Map. This technique can detect as few as 102 DNA copies and present high specifi city by negative amplification of other pathogenic bacterial species. This technique is sensitive, specific, rapid and does not require fluorescent probes or
post-PCR electrophoresis. Our technique is a possible new tool for the detection of Maa and Map infection in tissue specimens.
— We performed analysis of the biological properties of African swine fever virus (ASF) isolate Odintsovo 02/14. Domestic pigs were inoculated with 50 (low) or 5000 (high) hemadsorbing doses (HAD) of the virus via intranasal (IN) or intramuscular (IM) routes, to investigate the pathogenesis of ASF virus Odintsovo 02/14 isolate. Our results indicated that filtered 10% spleen suspension of ASFV isolate Odintsovo 02/14 induced an acute disease in pigs, resulting in 100% mortality rate. For cultural viral suspension (3rd passage), produced in a PBM cells mortality rate was 85.7%. We also present an analysis of the complete genome of African swine fever virus (ASF) Odintsovo 02/14 isolate. It is 189 333 nucleotide long and contains more than 160 open reading frames (ORFs). Complete nucleotide sequence of the genome of Odintsovo 02/14 isolate was obtained using pyrosequencing method and used to determine differences between the nucleotide sequences in the genomes of Odintsovo 02/14 and Georgia 01/2007. The genome of ASF virus Odintsovo 02/14 contains substitutions, insertions and deletions in genes encoding structural, membrane, and regulatory proteins, DNA reparation enzymes, host immune response evasion proteins, and MGF genes. The intergenic region I73R/I329L of Odintsovo 02/14 isolate contains 10-nucleotide long tandem repeat sequence, missing in Georgia 01/2007. Keywords— African swine fever (ASF), experimental challenge, complete genome sequencing, intergenic region, tandem repeats.
Presentation 6: Vibrio parahaemolyticus: genome plasticity, mobile genetic el...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Assesment of the Risk of Incursion of Exotic FMD viruses into Southeast Asia ...EuFMD
The 2018 Open Session of the EuFMD Standing Technical Committee was held in Borgo Egnazia - Italy, 29-31 October 2018 . The session theme surrounded increasing the supply of effective FMD vaccines.
The European Commission for the Control of Foot-and-Mouth Disease (EuFMD), one of FAO’s oldest Commissions, came into being on the 12th June 1954, with the pledge of the sixth founding member state to the principles of a coordinated and common action against Foot-and-mouth Disease.
Antimicrobial resistance (AMR) in N. gonorrhoeae (GC) - global problem but v...Игорь Шадеркин
Antimicrobial resistance (AMR) in N. gonorrhoeae (GC) - global problem but valid data are lacking in many geographic areas
Magnus Unemo, PhD, Assoc. Professor
Reference Laboratory for Pathogenic Neisseria
Department of Clinical Microbiology
Örebro University Hospital
Sweden
Similar to OS20 - Genetic characterization of serotype and genetic relatedness of foot-and-mouth disease viruses in South East Asia - HyunJi Lee (20)
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
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Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
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TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
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New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
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Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...
OS20 - Genetic characterization of serotype and genetic relatedness of foot-and-mouth disease viruses in South East Asia - HyunJi Lee
1. 1EuFMD | Open Session special edition | #OS20se
HyunJi Lee
Animal and Plant Quarantine Agency, Foot-and-mouth disease division /
Republic of Korea.
GENETIC CHARACTERIZATION OF
SEROTYPE AND GENETIC RELATEDNESS
OF FOOT-AND-MOUTH DISEASE VIRUSES
IN SOUTH EAST ASIA
2. 2EuFMD | Open Session special edition | #OS20se
Figure 2. The spreading of FMDV from Southeast Asia
into South Korea As exchanges between South Korea
and Southeast Asia have increased, the likelihood that
viruses from Southeast Asia will spread to South
Korea will increase.
Foot-and-mouth disease (FMD) is the most important disease of livestock, causing huge economic
impact on worldwide. FMD virus is a single strand of positive-sense RNA and over 8,000 bases in length.
The causative agent, FMD virus (FMDV), is divided into seven Serotype categories: A, O, C, Asia1, SAT1,
SAT2 and SAT3 based on VP1 sequence. Each serotype is divided into several topotypes and lineages by
genetic characterization. FMDV serotype O and A are the most prevalent in Southeast Asia containing
Vietnam, Laos, Cambodia, and Myanmar. Two different lineages of serotype O have been circulating in
Southeast Asia during 2018-2019; O/ME-SA/PanAsia and O/SEA/Mya-98. And A/ASIA/SEA97 of serotype
A also have been circulating in Southeast Asia. In Korea, FMDV serotype O/ME-SA/PanAsia was reported
in 2000 and 2002. O/SEA/Mya-98 was reported in 2010, 2014 and 2016, and A/ASIA/SEA97 was
reported in 2010 and 2018. With the recent increase in physical and human exchanges with Southeast
Asia, the possibility of introducing the FMDV serotype O/ME-SA/PanAsia into South Korea is increasing.
In this study, we collected 95 clinical FMDV samples between 2018 and 2019 in Vietnam, Laos and
Cambodia, analyzed genetic relatedness.
Figure 1. In South Korea, 11 foot-and-mouth disease cases have occurred since 2000, and only serotype O
and A have occurred.
Introduction
3. 3EuFMD | Open Session special edition | #OS20se
Figure 3. 95 clinical samples were collected that occurred between 2018 and 2019 from Vietnam, Laos
and Cambodia. Viral RNAs were extracted from the clinical samples and cells using a Nextracter
(Genolution). The 3D region in the virus genome was confirmed by qRT-PCR to determine whether the
collected samples were FMDV positive. The VP1 regions were amplified using a one-step RT-PCR
kit(Qiagen). PCR products were purified with ExoSAP-IT (USB) and directly sequenced on an ABI 3130
genetic analyzer(Applied Biosystems) using a Big Dye Terminator Kit v3.1(Applied Biosystems).
VP1 genome sequences were alinged by BioEdit and analyzed Phylogenetic tree estimated using the
Maximum-Likelihood method on the General Time Reversible model in MEGA-X version 10.0.1.
Figure 4.
A) It was possible to determine whether FMD was
positive or negative through 3D qRT-PCR. As a
result of analyzing 95 FMDV samples, 40 were
found to be FMD positive.
B) Major capsid protein, VP1 were amplified using
RT-PCR. Amplified PCR products were separated by
gel electrophoresis, stained with ethidium bromide.
A
B
FMDV from Vietnam, Laos, Cambodia (3D qRT-PCR)
Materials and Methods Results
4. 4EuFMD | Open Session special edition | #OS20se
Figure 5. Phylogenetic analysis of FMD positive samples from Vietnam, LAO, and
Cambodia. As a result of genome analysis based on the VP1 gene, Cambodia's
A/ASIA/SEA-97 and Vietnam's O/SEA/Mya98 each belong to one genetic cluster.
Some FMDVs in Cambodia and Vietnam and FMDV in Laos (n=1) belong to three
different genetic clusters in O/ME-SA/Panasia.
Results
FMDVs in Vietnam and Laos belonged to Serotype O and
classified into O/Me-SA/Panasia and O/SEA/Mya-98 genotypes.
FMDVs in Cambodia belonged to Serotype O and A, being
classified into O/ME-SA/Panasia and A/ASIA/SEA-97 genotype.
The further study may require investigation of antigenic
relatedness of the field FMDVs to strains of commercial FMD
vaccines. Also, Securing FMDV in a Pool 1 region and
accumulating the genetic information of the virus can provide
valuable information for further research on the genetic
diversity and antigenic relationship of FMDV to prevent and
control foot-and-mouth disease in South Korea in the future.
Conclusion