OS16 - Open Session 2016 Cascais, Portugal 26 - 28 /10/2016 EuFMD Sessions\Open Session\Archive-2018\Open 2016 Cascais- Portugal\PPT presentations\

1. Open Session of the EuFMD - Cascais –Portugal 26-28 October 2016
Detection and molecular
characterization of FMD viruses from
outbreaks in Northern Nigeria
2013-2015
D.O. Ehizibolo, A. Haegeman, A. De Vleeschauwer, J.U.
Umoh3, H.M. Kazeem, E.C. Okolocha, K. De Clercq

2. Nigeria
- Movement of cattle in the country and
across the borders (import, nomadic
pastoralism)
- Veterinary service: no control
programme for FMD
- FMD vaccination when vaccine is
available
- No systematic surveillance
- Notifications of FMD are sporadic
-> Need for better understanding of the
current epidemiology of FMD

3. Sampling reported FMD outbreaks 2013 - 2015

4. Bovine sera
State Location Number of
herds
Numbers of samples postive / number tested
NSP SPCE serotype
O A SAT1 SAT2 SAT3
Kaduna Zaria 1 25/25 25/25 19/25 3/25 9/25 9/25
Plateau Rukuba 2 5/9 4/4 2/4 NT 3/4 2/4
Kadisi 5 39/50 38/39 16/39 NT 26/39 11/39
Bauchi Magama 3 22/28 9/22 7/22 NT 9/22 NT
Goskayi 7 38/55 25/35 14/35 NT 5/35 NT
Kwara Ilorin 7 27/38 25/27 3/27 NT 21/27 2/27

5. Ovine sera
State Location Number of
herds
Numbers of samples postive / number tested
NSP SPCE serotype
O A SAT1 SAT2 SAT3
Plateau Rukuba 1 4/7 0/4 0/4 NT 2/4 1/4
Kadisi 2 6/6 6/6 0/6 NT 3/6 NT
Bauchi Magama 3 10/15 3/9 6/9 NT 7/9 NT
Goskayi 4 21/39 15/21 5/21 3/21 1/21 3/21
Kwara Ilorin 1 6/6 3/6 0/6 NT 2/6 0/6

6. Bovine tissue samples
State Location Number of
herds affected
Sample type Number positive/tested AgELISA
FMDV serotyperRT-PCR VI
Kaduna Zaria 1 Gum ep. 8/8 6/8 O
Kagoro 1 Tongue ep. 2/2 2/2 A
Plateau Rukuba 2 Gum ep. 10/10 5/10 O, SAT1
Riyom 1 Gum ep. 6/6 3/6 O, SAT1
Bukuru NR Gum ep. 9/9 9/9 O, SAT1, SAT3
Mikang 1 Gum ep. 2/2 2/2 SAT2
Dawarki 1 Tongue ep. 4/4 4/4 SAT2
Barkin ladi 1 Gum ep. 2/2 0/2 NT
Bauchi Magama 6 Gum ep. 4/5 2/5 O, SAT1
Tongue ep. 10/10 4/10 SAT2
Rugan Toro 1 Tongue ep. 3/3 2/3 A, SAT1
Baram-gada 5 Tongue ep. 7/7 3/7 A, SAT1
Kwara Ilorin 1 Gum ep. 1/1 0/1 NT

7. Neighbor-joining method
Tamura-Nei model
1000 bootstraps
Uniform rates among sites
Pairwise deletions of gaps
Serotype A

8. Serotype O
Neighbor-joining method
Tamura-Nei model
1000 bootstraps
Uniform rates among sites
Pairwise deletions of gaps
WA
EA-3

9. Neighbor-joining method
Tamura-Nei model
1000 bootstraps
Uniform rates among sites
Pairwise deletions of gaps
Serotype SAT2
VII

10. Sampling of an FMD outbreak in Jos South 2015

11. Serotype SAT1
X
SAT1/TAN/2/99(KJ999931)
SAT1/K21/06(HQ267528)
SAT1/KEN/22/08(KR188455)
SAT1/TAN/22/12(KM268899)
SAT1/KEN/127/11(KP263445)
SAT1/K127/2011(KP263445)
SAT1/K/159/12(KP263444)
SAT1/KEN/98/10(KR188479)
SAT1/KEN/140/10(KR188512)
SAT1/KEN/78/10(KR188471)
SAT1/KEN/10/06(KR188454)
SAT1/K67/05(HQ267526)
SAT1/KEN/1/05(KR188452)
SAT1/KEN/9/91(AY441995)
SAT1/K/110/81(HQ267521)
SAT1/TAN/2/71(KF561707)
SAT1/TAN/1/96(KF561710)
SAT1/TAN/13/74(AY442001)
SAT1/TAN/3/80(KF561709)
SAT1/TAN/2/77(KF561708)
SAT1/ZAM/1/06(KJ999930)
SAT1/ZIM/23/03(HQ268527)
SAT1/MOZ/3/02(|DQ009720)
SAT1/SAR/9/81(DQ009715)
SAT1/SAR/2/09(KJ999912)
SAT1/KNP/07/03/1(DQ780590)
SAT1/RV/11/37(AY593839)
SAT1/UGAB/21/70(KF219682)
SAT1/UGA/7/99(AY442011)
SAT1/UGA/3/99(AY442009)
SAT1/UGAB/10/70(KF219681)
SAT1/UGA/161/13(KP025679)
SAT1/UGA/116/13(KP025678)
SAT1/BOT/2/98(KJ999918)
SAT1/1bech/ISO30(AY593838)
SAT1/BOT/1/06(KJ999919)
SAT1/NAM/1/10(KJ999923)
SAT1/ZIM/6/94(GU194500)
SAT1/SA/43/61(AY593842)
SAT1/SWA/40/61(AY593843)
SAT1/BOT/1/77(KF219686)
SAT1/BOT/1/68(Z98203)
SAT1/UGA/1/97(AY442012)
SAT1/NIG/1/15
SAT1/NIG/2/15
SAT1/NGR/5/76(AF431720)
SAT1/NIG/6/76(AF431716)
SAT1/NGR/2/76(AF431718)
SAT1/NIG14/76(AF431725)
SAT1/ETH/3/07(FJ798154)
SAT1/ETH/21/07(FJ798157)
SAT1/ETH/18/07(FJ798155)
SAT1/UGA/13/74(AY442010)
SAT1/SUD/9/74(AY441999)
SAT1/SUD/8/74(AY441998)
SAT1/SUD/13/74(AY442000)
SAT1/SUD/4/76(AY441997)
SAT1/NIG/2/79(AF431728)
SAT1/NIG/3/80(AF431729)
SAT1/NIG/10/81(AF431731)
100
100
76
100
59
100
100
98
95
70
95
100
75
100
76
96
62
93
100
100
74
65
61
86
51
99
100
100
62
100
75
100
100
90
97
98
69
64
0.05
I
II
IV
III
VIII
X
V
IX
VII
VI
Maximum likelihood method
Tamura-Nei model
1000 bootstraps
VIII
III
V

12. Thank you
OIE Laboratory
Twinning Project
CODA-CERVA
Veterinary and Agrochemical Research Centre