1. Adeno-associated virus in human gene therapy
Somesh R. Doddi
B.A. Biochemistry
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11. AAV2 plasmid expression pRepCap construct; aav helper
cassette w/foreign gene of plasmid
interest; vector plasmid
Rep; Cap:
Gene: • 2;5 (lung cancer)
• P130 (lung cancer, liver • 2;3 (liver cancer)
cancer, quiescence) • 2;2 (insulin resistant ‘type
• E1a (lung cancer, liver 2’diabetes, HIV/AIDS)
cancer, apoptosis;
*3Hthymidine)
• Acrp30 (insulin resistant
‘type 2’diabetes)
• 2,5-dkgr (HIV/AIDS)
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15. Thank You 15