1. The document discusses the history and concepts of optical isomerism, including the discovery of enantiomers by Pasteur and the proposal of the tetrahedral carbon model by van't Hoff.
2. It defines key terms like enantiomers, diastereomers, and meso compounds. Enantiomers are non-superimposable mirror images while diastereomers are stereoisomers that are not mirror images. Meso compounds have an internal plane of symmetry and are optically inactive.
3. Methods for separating enantiomers like crystallization and forming diastereomeric salts are described. The importance of enantiomers in biology is highlighted, as most biomolecules and
…….. “DRUGS” do something in our body as a result of their molecular structure, which determines:
1. Physicochemical properties
2. Chemical / biochemical reactivity
3. Shape
4. STEREO-CHEMISTRY
Here you will find about optical isomerism...
To know about more optical isomerism download it and then you will find a link... open this and go through..
…….. “DRUGS” do something in our body as a result of their molecular structure, which determines:
1. Physicochemical properties
2. Chemical / biochemical reactivity
3. Shape
4. STEREO-CHEMISTRY
Here you will find about optical isomerism...
To know about more optical isomerism download it and then you will find a link... open this and go through..
Biphenyl derivatives & Atropisomerism:Optical activity in Biphenyls, Stereochemistry of biphenyl derivatives, rules and assigning RS configuration to biphenyls
Conformational isomerism
Conformations of Ethane:
Conformations of Cyclohexane
Representation of conformations:
Sawhorse projection:
Newman projection:
The conformational analysis of cyclohexane
CONFORMATION OF MONO SUBSTITUTED CYCLOHEXANE
Introduction to stereochemistry, Representation of 3D molecules, R/S nomenclature, D-L and M-P convention, Topicity, Prochirality, Allenes, Biphenyls, Spiranes, Hemispirane.
Biphenyl derivatives & Atropisomerism:Optical activity in Biphenyls, Stereochemistry of biphenyl derivatives, rules and assigning RS configuration to biphenyls
Conformational isomerism
Conformations of Ethane:
Conformations of Cyclohexane
Representation of conformations:
Sawhorse projection:
Newman projection:
The conformational analysis of cyclohexane
CONFORMATION OF MONO SUBSTITUTED CYCLOHEXANE
Introduction to stereochemistry, Representation of 3D molecules, R/S nomenclature, D-L and M-P convention, Topicity, Prochirality, Allenes, Biphenyls, Spiranes, Hemispirane.
Basic concepts of organic chemistry such as structural formulas, different kinds of representation, types of isomerism, examples, alkanes, alkenes, alkynes etc.
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As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
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In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
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The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
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PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
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The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
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Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
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Cheryl Hung, ochery.com
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Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
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2. Content History Enantiomers Reasons for molecular handedness Optical activity of enantiomers Naming Conventions Diastereomers Meso Compounds Resolution of Enantiomers Importance of Enantiomers 2
5. Discovery of Optical Activity In 1850, French Physicist Jean-BaptiseBiot observed that solutions of some organic compounds like sugar and camphor have the ability to rotate plane polarized light Up till then the basis of this phenomenon was not yet known 5
6. Separation of Enantiomers In 1848 French Chemist Louis Pasteur separated a solution of optically inactive tartaric acid into two optically active components. He observed that: Each of these components had identical physical properties like density, melting point, solubility, etc But One of the components rotated plane polarized light clockwise while the other component rotated the polarized light by the same amount counter clockwise. 6
7. Pasteur made a proposal that since the crystals of tartaric acid were mirror images of each other, their molecules were also mirror images of each other 7
8. Additional research done by Pasteur revealed that one component of tartaric acid could be utilized for nutrition by micro-organisms but the other could not. Thus Pasteur concluded that biological properties of chemical substances depend not only on the nature of the atoms comprising the molecules but also on the manner in which these atoms are arranged in space. 8
9. The tetrahedral Carbon In 1874 as a student at University of Utrecht, Jacobusvan't Hoff proposed the tetrahedral carbon. His proposal was based upon evidence of isomer number: Conversion of CH4 into CH3R(1 isomer) Conversion of CH3R into CH2RR1(1 isomer) van't Hoff realized that the four hydrogens in CH4 had to be equivalent and a geometrical square was ruled out because it would form 2 isomers for CH2RR1. Thus he proposed the tetrahedral carbon centre. 9
10. Explanation of Optical Isomerism The tetrahedral carbon not only collaborated the absence of isomers in CH2YZ, but also predicted the existence of mirror image isomers. When carbon makes four single bonds with four different groups such as CHFClBr, non-super-imposable mirror-image molecules exist 10
12. Definition An enantiomer is one of two stereoisomers that are mirror images of each other and are non-superimposable. 12
13. As seen here, the two molecules of lactic acid are mirror images of each other, but cannot superimpose. 13 (S)-Lactic acid or (+)-Lactic acid (R)-Lactic acid or (-)-Lactic acid
14. The Tetrahedral Carbon Enantiomers result when two compounds that are mirror-images of each other cannot superimpose Eg. when a tetrahedral carbon is bonded to four different substituents(CR1R2R3R4) For example, in lactic acid, there are four different groups: -H, -OH, -CH3, -COOH Can you name other examples of enantiomers formed this way? Alanine, Tartaric Acid, Glyceraldehyde 14
16. Chirality A molecule that is not superimposable to its mirror image is said to be chiral The most common cause of chirality in organic molecules is the presence of a carbon atom bonded with 4 different groups(eg in lactic acid), this is also known as point chirality. These carbon atoms are called chirality centers or chirality centres. Can you think of other causes of Chirality? (be creative) 16
17. Identifying Chiral Molecules The obvious way: find any carbon with 4 different substituents. If there are any, then the molecule is chiral. Isotopic differences are also considered different substituents. Thus, -CH2-, -CH3(methyl), >C=O(carbonyl), >C=C<(alkene) and -C@C-(alkyne) groups cannot be chirality centers as they all have less than 4 substituents. Easier way: look for the presence of a plane of symmetry, since a symmetrical molecule is identical to its mirror image and is thus achiral 17
18. Drawing of Enantiomers Fischer Projection (do not confuse with Lewis Structure) Natta Projection (we all know this) For cyclic molecules Haworth Projection Chair conformation 18
21. As seen from Biot’s experiment, enantiomers can rotate plane polarised light, since they are optically active When a beam of plane polarised light passes through a solution of a non-racemic (scalemic) mixture (ie has enantiomeric excess of one of the enantiomers), rotation of the polarisation plane occurs. 21
23. Optical Activity Observed rotation The number of degrees, , through which a compound rotates the plane of polarized light. Dextrorotatory (+) Acompound that rotates the plane of polarized light to the right. Levorotatory (-) Refers to a compound that rotates the plane of polarized light to the left. 23
24. Specific Rotation Specific rotation refers to the observed rotation for a sample in a tube 1.0 dm in length and at a concentration of 1.0 g/mL. The degree of rotation also depends on the wavelength of the light (the yellow sodium D2 line near 589 nm is commonly used for measurements) 24
25. Calculating Specific Rotation (liquids) The specific rotation of a compound is given by: Where: [a] is the specific rotation T is the temperature l is the wavelength of light used l is the path length of sample (in decimetres) d is the density of the sample (in g/cm3 for pure liquids) The sign of the rotation (+ or -) is always given 25
26. Calculating Specific Rotation (liquids) The specific rotation of a compound is given by: Where: [a] is the specific rotation T is the temperature l is the wavelength of light used l is the path length of sample (in decimetres) c is the concentration of the sample (in g/cm3) The sign of the rotation (+ or -) is always given 26
27. Optical Purity Optical purity: A way of describing the composition of a mixture of enantiomers. Enantiomeric excess: The difference between the percentage of two enantiomers in a mixture. optical purity is numerically equal to enantiomeric excess, but is experimentally determined. 27
29. Types of naming R,S designation +, - designation (explained just now) d,l Nomenclature 29
30. R-, S- Naming Convention This convention labels each chiralcenterR or S according to a system by which its substituents are each assigned a priority based on atomic number. The priority of substituents are assigned based on Cahn–Ingold–Prelog priority rules 30
31. Cahn–Ingold–Prelog priority rules Assignment of Priority Compare the atomic number of the atoms directly attached to the chirality center. The group with the atom of higher atomic number receives higher priority. (eg –Cl > –OH > –NH2 > –CH3) If different isotopes of the same element is attached to the chiral center, the group with the higher mass number recieves higher priority (2H > 1H) 31
32. If double or triple bonded groups are encountered as substituents, they are treated as an equivalent set of single-bonded atoms.For example, C2H5– < CH2=CH– < HC≡C– 32
33.
34. If there is still a tie, each atom in each of the two lists is replaced with a sub-list of the other atoms bonded to it (at distance 3 from the chiralitycenter), the sub-lists are arranged in decreasing order of atomic number, and the entire structure is again compared atom by atom. This process is repeated, each time with atoms one bond farther from the chiralitycenter, until the tie is broken. 34
35. Identifying the Configuration After having ranked the four groups attached to the stereo-center, orientate the molecule such that the lowest ranking group points directly back Look at the 3 substituents left facing you: If the priority of the remaining three substituents decreases in clockwise direction, it is labeledR (for Rectus, Latin for right), if it decreases in counterclockwise direction, it is S (for Sinister, Latin for left). 35
36. d,l Nomenclature The d/l system names isomers after the spatial configuration of its atoms, by relating the molecule to glyceraldehyde. Glyceraldehyde is chiral itself, and its two isomers are labeled d and l This nomenclature is still used in certain organic compounds like saccharides and amino acids 36
38. Identifying the Configuration For Monosaccharides: The absolute configuration of all monosaccharides is denoted by the configuration at the chiralitycenter furthest from the anomeric centre (the carbonyl carbon in the open chain representation) If, in the Fischer projection, that centre has the hydroxyl group on the right, it is a d-sugar; if on the left, it is an l-sugar. By convention, the "D" and "L" symbols are written in small capitals. 38
39. Non amino-acids such as Lactic, Ascorbic, Tartaric Acid also follow the same rules as Saccharides. 39
40. Identifying the Configurations For Amino Acids CORN Rule: The groups: COOH, R, NH2 and H (where R is a variant carbon chain)are arranged around the chiralcenter carbon atom. Starting with the hydrogen atom away from the viewer, if these groups are arranged clockwise around the carbon atom, then it is the d-form. If counter-clockwise, it is the l-form. 40
41. Relation to other naming conventions The d/l labeling is unrelated to (+)/(−); it does not indicate which enantiomer is dextrorotatory and which is levorotatory. Rather, it says that the compound's stereochemistry is related to that of the dextrorotatory or levorotatory enantiomer of glyceraldehyde—the dextrorotatory isomer of glyceraldehyde is, in fact, the d- isomer. 41
43. 2n Rule As a general rule the maximum number of isomers for a compound is 2n, with n being the number of chirality centers. *As seen in the next slide 43
45. Definition Diastereomers refer to enantiomers that are not mirror images of each other They can be chiral or achiral Eg D-glucose and D-galactose 45
46. Stereochemistry in Sugars Aldose An aldose is a monosaccharide that contains only one aldehyde group per molecule. The chemical formula takes the form Cn(H2O)n. The maximum number of chirality centers for any aldose is 2n-2 Since the “head”(-CH=O) and “tail”(-CH2OH) carbons cannot be chiral centers 46
47. Examples (Aldotetrose) Aldotetroses are Aldoses with 4 carbons As such, the maximum number of enantiomers of aldotetroses is 4 (24-2) The 4 possible enantiomers of Aldotetroses 47
49. Reasons As we can see, although Erythrose and Threose are stereoisomers, they are not enantiomers, since their molecules are not mirror-images of each other. They are thus called diastereomers 49 Erythrose Threose They are not mirror images of each other
50. This is because stereoisomers are only enantiomers because all their chirality centers have opposite configurations, but if not all chiralitycentres have opposite configurations, then they are diastereomers. 50 Example of d(+)-Glucose and D(+)-Galactose
52. Definition A meso-compound is a non-optically active member of a set of stereoisomers. It does not give a (+) or (-) reading on a polarimeter It is non-optically active as it is achiral It is achiral since it has a plane of symmetry 52
54. Mesotartaric Acid Let’s assume the mirror image of mesotartaric acid: Flip the molecule: And you find that you get the same molecule 54 Flip this molecule 180 degrees
55. Meso Compounds Thus, we can say that mesotartaric acid is not an enantiomer, as it is superimposible with its mirror image. This is due to the fact that there is a plane of symmetry in the molecule 55 There is a plane of symmetry Rotate this bond 180 degrees
57. Resolution of Enantiomers In the lab, if we make chiral compounds from achiral starting materials, we are bound to get a racemic mixture. The way we separate the mixture is known as resolution Resolution is important as most of the time enantiomerically pure compounds are required 57
58. Crystallisation One way of resolution would be crystallisation (like what Pasteur did), it’s use is limited to solid compounds (eg Sodium Ammonium Tartrate) It does not work for liquid compounds that do not crystallise under ordinary conditions 58
59. The most common way of resolution would be to use an acid-base reaction between a racemic mixture of chiral carboxylic acid and an amine base to yield an ammonium salt, which can be crystallised. 59 +
60. Resolution of enantiomers Another way would be using a chemical reaction to produce a diastereomer. This can be done in various ways, by esterification or by forming diastereomeric salt (Adduct). 60
61. Resolution of enantiomers (Esterification) One way of resolution of enantiomers would be using an ester 61 TsOHor H2SO4 + + Chiral but enantiomerically pure Diastereomers Chiral but Racemic
62. Diastereomers have different physical properties (solubility, boiling point etc), and should be quite easy to separate by distillation/crystallisation/chromatography When a pure enantiomer (diastereomer) is separated, the original intended products can be obtained 62 Enantiomerically pure product NaOH, H2O +
63. Resolution of enantiomers (Forming a diastereomeric salt) When a racemic mixture of a carboxylic acid reacts with a single enantiomer of a chiral base, diastereomers are obtained. Diastereomers can then be separated just like previously 63
64. Acidification of the two diastereomeric salts resolved the original racemic mixture. 64 Racemic Mixture Enantiomerically pure compound
66. Chirality in the Biological World Enzymes are like hands in a handshake The substrate fits into a binding site on the enzyme surface A left-handed molecule, like hands in gloves, will only fit into a left-handed binding site and a right-handed molecule will only fit into a right-handed binding site. Because of the differences in their interactions with other chiral molecules in living systems, enantiomers have different physiological properties. 66
67. Some Examples d-Glucose can be used for metabolism for all organisms but not l-glucose Almost all of the amino acids in proteins are (S) at the α carbon. In most pharmaceutical drugs, only one of the enantiomers are biologically active. Eg. (S)Ibuprofen is an active analgestic agent but the (R) enantiomer is biologically inactive 67 a carbon
68. Chirality in the Biological World A schematic diagram of an enzyme active site capable of binding with (R)-glyceraldehyde but not with (S)-glyceraldehyde. 68
69. Credits Most of these information are taken from online sources[citation needed] However due to the fact that the layout was copied from the book, we apologise for the fact that this may be a little boring (for some) Done by: Jeff Xu, Luther Mok, Joshua Lay, Wen Song Design by Jonathan Yong 69