This document discusses various methods for classifying and identifying microorganisms, including serology techniques like slide agglutination and ELISA that combine antiserum with unknown bacteria, Western blot, and phage typing. It also covers genetic methods such as analyzing DNA base composition, DNA fingerprinting using restriction enzymes, rRNA sequencing, PCR, nucleic acid hybridization using DNA probes and chips, and phylogenetic techniques like dichotomous keys, cladograms, and analyzing differences in electrical conductivity and fluorescence between species.

1. Chapter 10, part B Classification of Microorganisms

2. Numerical Identification Figure 10.9

3. Combine known antiserum + unknown bacterium Slide agglutination ELISA Western blot Serology Figure 10.10

4. Western Blot Figure 10.12

5. Phage Typing Figure 10.13

6. Differences in electrical conductivity between species Fluorescence of some species Cells selectively stained with antibody + fluorescent dye Flow Cytometry Uses Figure 18.11

7. DNA base composition Guanine + cytosine moles% (GC) DNA fingerprinting Electrophoresis of restriction enzyme digests rRNA sequencing Polymerase Chain Reaction (PCR) Genetics Figure 10.14

8. Nucleic Acid Hybridization Figure 10.15

9. Nucleic Acid Hybridization: DNA probe Figure 10.16

10. Nucleic Acid Hybridization: DNA chip Figure 10.17

11. Figure 10.5

12. Dichotomous Key

13. Cladogram Figure 10.18.1

14. Cladogram Figure 10.18.2