16 August 2010 Musculoskeletal-Integumentary Module Histology Laboratory Muscles

1. MUSCLE Lab # 3 August 16, 2010 ASMPH 2015

2. SKELETAL MUSCLE (STRIATED MUSCLE)

3. Striated Muscle l.s. On yellow pointers: NUCLEUS of the Muscle

4. Striated Muscle l. s. Dark Band (A band): very fine light space in between is the H band, where the M line is found. Light Band (I band): very light line in between is the Z line Middle of light band to another middle of light band (Z line to Z line) = sarcomere Observe the alternating light and dark bands Multi-nucleated

5. Striated Muscle l. s. Light Band (I band): very light line in between is the Z line Dark Band (A band): very fine light space in between is the H band, where the M line is found. Middle of light band to another middle of light band (Z line to Z line) = sarcomere Nucleus

6. Sarcomere: from the Net

9. Striated Muscle (Scanner 40x) Perimysium Blood vessel

10. Striated Muscle (LPO 100x) Perimysium Endomysium Fascicle Blood vessel

14. SMOOTH MUSCLE

15. Smooth muscle l.s. Note: Spindle shaped cell, centrally located nucleus and eosinophilic cytoplasm

16. Smooth Muscle c.s. l.s.

20. Duodenum HD168 Smooth muscles of the Outer wall of the GIT Inner circumferential (circular) layer Outer longitudinal layer

21. Esophagus HD159A Lower Region Smooth muscles of the Outer wall of the GIT Outer longitudinal layer Inner circumferential (circular) layer Muscularis propria

22. Smooth Muscle: Jejunum (LPO)

23. Smooth Muscle: Jejunum (HPO) L.S.

24. Smooth Muscle: Jejunum (HPO)

25. ILEUM (4x) Outer longitudinal layer Inner circumferentially-arranged layer

26. ILEUM (10x) Outer longitudinal layer Inner circumferentially-arranged layer

27. ILEUM (40x) Outer longitudinal layer Inner circumferentially-arranged layer

28. Epithelial Layer Connective Tissue Layer Inner Smooth Muscle Layer Outer Smooth Muscle Layer Large Intestine

29. Large Intestine Outer longitudinal layer of smooth muscle Inner circumferential layer of smooth muscle

30. Large Intestine, HPO Inner circumferential layer of smooth muscle

31. Large Intestine, HPO Outer longitudinal layer of smooth muscle

32. Smooth Muscle: Arrector Pili Shown on pointer - Part of the pilosebaceous unit (with hair follicle and sebaceous gland) - Makes hair stand on end

33. Smooth Muscle: Arrector Pili On pointer: Close-up of arrector pili muscle

34. ARTERY AND VEIN [LPO] Smooth Muscle

35. SMOOTH MUSCLE - ARTERY AND VEIN [HPO] Tunica Media Smooth Muscle cells (and elastic fibers) ARTERY AND VEIN [HPO] Tunica Intima - Endothelium and elastic fibers Tunica Adventitia - collagen and elastic fibers

36. CARDIAC MUSCLE

37. Cardiac Muscle Heart (l.s.) Nuclei Intercalated Discs