Project Overview
•
0 likes•161 views
The document discusses protein kinase C (PKC) isoforms, specifically the atypical PKC (aPKC) isoforms ι and ζ. It notes that aPKCι and ζ share well-defined protein domains, including PB1, C1, and kinase domains, and are 72% similar at the amino acid level. Studies have found that aPKCι knockout is lethal, while aPKCζ knockout has milder effects, and that the isoforms have different roles in processes like cell polarity and tumor formation. Deletion of the kinase domain causes aPKCι, but not aPKCζ, to enter the nucleus, suggesting the isoforms have different shuttling
Report
Share
Report
Share
Recommended
Dna And Stuff (JC July 31, 2008)
1. The document discusses DNA replication and the cell cycle checkpoint processes that ensure DNA is properly replicated. It specifically focuses on proteins involved like CHK1 and CHK2 kinases.
2. CHK1 plays a key role in the G2/M checkpoint and inhibition of CHK1 can sensitize tumors to treatments like radiation and chemotherapy.
3. The document includes figures and diagrams illustrating concepts like the relationship between origin firing and fork velocity, and how DNA damage checkpoints work at the single cell level.
KL PhD Presentation
1. The document discusses systems biology approaches to modeling the endothelial cell response to fluid shear stress. It describes experimental techniques to apply controlled fluid shear stress and measure downstream cellular responses.
2. Mathematical models are formulated to represent molecular interactions and pathways involved in the shear stress response. Model predictions are compared to experimental data to validate and refine the models.
3. The models can provide insights into critical components, feedback loops, and how external perturbations may influence the cellular response to shear stress. Further experimental validation of model predictions is needed.
Regulation of cell cycle (1)
The cell cycle is tightly regulated by cyclins and cyclin-dependent kinases (Cdks). Cyclins bind to and activate Cdks at specific points in the cell cycle to promote progression. Cyclins fluctuate in concentration throughout the cycle while Cdk levels remain constant. Rb protein and p53 tumor suppressor proteins also regulate the cell cycle by inhibiting progression from G1 to S phase when DNA damage is detected. Checkpoints at G1/S and G2/M ensure conditions are suitable before allowing division to proceed and prevent errors from being passed on. Dysregulation of the cell cycle, as seen in cancer, results from defects in these regulatory proteins and checkpoints.
090630 Ion Cannel Retreat
The document discusses the use of pluripotent stem cell-derived cardiomyocytes as substrates for automated ion channel and electrophysiological analysis systems. Specifically, it describes how cardiomyocytes can be selected from mouse embryonic stem cells using puromycin, and how these cells express appropriate cardiac ion channels. It also demonstrates that the cardiomyocytes can be used on automated patch clamp systems like PatchXpress and QPatch to measure cardiac ion currents like INa, ICa, and IK, and to study the effects of drugs on these currents. The document establishes pluripotent stem cell-derived cardiomyocytes as a physiologically relevant and reproducible model for high-throughput electrophysiology screening.
NiH_Presentation
1) The document summarizes research into genetic interactors of the BRCA2 tumor suppressor gene, which is linked to hereditary breast cancer. A retroviral screening identified BRE as a genetic interactor that rescues lethality in BRCA2-deficient cells.
2) Further experiments showed that BRE overexpression in BRCA2-deficient cells leads to increased levels of the Cdc25A cell cycle regulator after DNA damage, preventing cell cycle arrest.
3) BRE was found to interact with the transcription factor ATF3 and induce transcription of Cdc25A. Reporter assays aim to identify the role of ATF3 binding sites on the Cdc25A promoter in regulating its transcription
Experimental manipulation of curli
This document describes several experimental procedures used to study curli production and biofilm formation in bacteria:
1) Congo Red assays and western blot analysis are used to assess curli production in bacterial strains.
2) Purification methods are described to isolate curli subunits from bacterial cells, either under native or denaturing conditions.
3) Polymerization assays use purified curli subunits and fluorescent dyes to study fiber formation and seeding processes in vitro.
4) Biofilm formation assays examine the role of curli in supporting ring-shaped and pellicle biofilms at the air-liquid interface.
Cancer ppt 2
1. Several viruses can cause cancer by inserting oncogenes into host cell DNA or expressing viral proteins that disrupt normal cell cycle regulation. This includes hepatitis B and C viruses, papillomaviruses like HPV16/18, and herpesviruses like EBV.
2. Retroviruses like HTLV-1 and oncogenic genes they carry, like src, can also cause cancerous transformation when expressed in host cells. Proto-oncogenes normally involved in cell signaling can become oncogenes when mutated or abnormally expressed.
3. Common oncogenic mutations involve genes in the Ras, Myc, and Bcr-Abl pathways, disrupting normal cell cycle controls and
Seminario Biologia Molecular
Rv1075c is a conserved hypothetical protein in Mycobacterium tuberculosis that shares similarities to bacterial lipases and esterases. A study identified that Rv1075c has esterase activity, preferring short-chain fatty acids like acetate. Mutation of Rv1075c led to reduced bacterial growth in macrophages and mice. The study exposed M. tuberculosis strains expressing wild-type or mutated Rv1075c to stress conditions and infected macrophages and mice to determine Rv1075c expression and effects on infection. Results demonstrated Rv1075c induction during low pH stress and its role in M. tuberculosis survival and virulence through fatty acid hydrolysis.
Recommended
Dna And Stuff (JC July 31, 2008)
1. The document discusses DNA replication and the cell cycle checkpoint processes that ensure DNA is properly replicated. It specifically focuses on proteins involved like CHK1 and CHK2 kinases.
2. CHK1 plays a key role in the G2/M checkpoint and inhibition of CHK1 can sensitize tumors to treatments like radiation and chemotherapy.
3. The document includes figures and diagrams illustrating concepts like the relationship between origin firing and fork velocity, and how DNA damage checkpoints work at the single cell level.
KL PhD Presentation
1. The document discusses systems biology approaches to modeling the endothelial cell response to fluid shear stress. It describes experimental techniques to apply controlled fluid shear stress and measure downstream cellular responses.
2. Mathematical models are formulated to represent molecular interactions and pathways involved in the shear stress response. Model predictions are compared to experimental data to validate and refine the models.
3. The models can provide insights into critical components, feedback loops, and how external perturbations may influence the cellular response to shear stress. Further experimental validation of model predictions is needed.
Regulation of cell cycle (1)
The cell cycle is tightly regulated by cyclins and cyclin-dependent kinases (Cdks). Cyclins bind to and activate Cdks at specific points in the cell cycle to promote progression. Cyclins fluctuate in concentration throughout the cycle while Cdk levels remain constant. Rb protein and p53 tumor suppressor proteins also regulate the cell cycle by inhibiting progression from G1 to S phase when DNA damage is detected. Checkpoints at G1/S and G2/M ensure conditions are suitable before allowing division to proceed and prevent errors from being passed on. Dysregulation of the cell cycle, as seen in cancer, results from defects in these regulatory proteins and checkpoints.
090630 Ion Cannel Retreat
The document discusses the use of pluripotent stem cell-derived cardiomyocytes as substrates for automated ion channel and electrophysiological analysis systems. Specifically, it describes how cardiomyocytes can be selected from mouse embryonic stem cells using puromycin, and how these cells express appropriate cardiac ion channels. It also demonstrates that the cardiomyocytes can be used on automated patch clamp systems like PatchXpress and QPatch to measure cardiac ion currents like INa, ICa, and IK, and to study the effects of drugs on these currents. The document establishes pluripotent stem cell-derived cardiomyocytes as a physiologically relevant and reproducible model for high-throughput electrophysiology screening.
NiH_Presentation
1) The document summarizes research into genetic interactors of the BRCA2 tumor suppressor gene, which is linked to hereditary breast cancer. A retroviral screening identified BRE as a genetic interactor that rescues lethality in BRCA2-deficient cells.
2) Further experiments showed that BRE overexpression in BRCA2-deficient cells leads to increased levels of the Cdc25A cell cycle regulator after DNA damage, preventing cell cycle arrest.
3) BRE was found to interact with the transcription factor ATF3 and induce transcription of Cdc25A. Reporter assays aim to identify the role of ATF3 binding sites on the Cdc25A promoter in regulating its transcription
Experimental manipulation of curli
This document describes several experimental procedures used to study curli production and biofilm formation in bacteria:
1) Congo Red assays and western blot analysis are used to assess curli production in bacterial strains.
2) Purification methods are described to isolate curli subunits from bacterial cells, either under native or denaturing conditions.
3) Polymerization assays use purified curli subunits and fluorescent dyes to study fiber formation and seeding processes in vitro.
4) Biofilm formation assays examine the role of curli in supporting ring-shaped and pellicle biofilms at the air-liquid interface.
Cancer ppt 2
1. Several viruses can cause cancer by inserting oncogenes into host cell DNA or expressing viral proteins that disrupt normal cell cycle regulation. This includes hepatitis B and C viruses, papillomaviruses like HPV16/18, and herpesviruses like EBV.
2. Retroviruses like HTLV-1 and oncogenic genes they carry, like src, can also cause cancerous transformation when expressed in host cells. Proto-oncogenes normally involved in cell signaling can become oncogenes when mutated or abnormally expressed.
3. Common oncogenic mutations involve genes in the Ras, Myc, and Bcr-Abl pathways, disrupting normal cell cycle controls and
Seminario Biologia Molecular
Rv1075c is a conserved hypothetical protein in Mycobacterium tuberculosis that shares similarities to bacterial lipases and esterases. A study identified that Rv1075c has esterase activity, preferring short-chain fatty acids like acetate. Mutation of Rv1075c led to reduced bacterial growth in macrophages and mice. The study exposed M. tuberculosis strains expressing wild-type or mutated Rv1075c to stress conditions and infected macrophages and mice to determine Rv1075c expression and effects on infection. Results demonstrated Rv1075c induction during low pH stress and its role in M. tuberculosis survival and virulence through fatty acid hydrolysis.
Molecular markers and plant breeding4
This document discusses the techniques of restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR). RFLP involves using restriction enzymes to cut DNA into fragments, which are then analyzed via gel electrophoresis and probing. PCR, developed in 1985, allows for exponential amplification of specific DNA regions using oligonucleotide primers, DNA polymerase, and thermal cycling. The PCR process involves repeated cycles of template DNA denaturation, primer annealing, and fragment extension, resulting in millions of copies of the target DNA. Both RFLP and PCR are useful for analyzing DNA samples.
Recombinant Antibody Overview I - Creative Biolabs
The document discusses recombinant antibody production. It begins by describing antibody structure and function, including the five main antibody isotypes found in vivo. It then details the process of natural antibody production by B cells, including B cell development, antibody gene rearrangement, and class switching. Finally, it introduces recombinant antibody production using hybridoma technology and lists the technical advantages of Creative Biolabs' hybridoma platform for generating recombinant antibodies.
Blood2007
This document summarizes the discovery of duplicated VegfA and KDR receptor genes in zebrafish that mediate vascular development. Specifically:
- The researchers identified a duplicated zebrafish VegfA gene (VegfAb) that encodes 171- and 210-amino acid isoforms not found in the single VegfA gene.
- They also found a duplicated KDR receptor gene (Kdrb) that encodes a receptor similar to mammalian KDR.
- Knockdown experiments in zebrafish showed that both VegfAb and the duplicated KDR receptor genes play important roles in vascular development.
- Further experiments demonstrated that the VegfAb isoforms are poorly secreted compared to VegfA isoforms
Imaginal discs1
The document summarizes a study on gene expression during regeneration of Drosophila wing imaginal discs. Microarray analysis found over 1,000 genes differentially expressed between cut and intact discs in the first 24 hours after wounding, indicating a strong initial response to injury. Many genes involved in apoptosis, stress response, cytoskeletal activity and JNK signaling showed increased expression. Between 24-72 hours, expression returned toward normal levels as discs resumed activities interrupted by wounding. The study identifies signaling pathways and gene categories important for disc regeneration.
Cancer
Cancer is an uncontrolled multiplication of cells that manifests as malignant or benign tumor, impairs immune system and produces fatal intoxicants.
The effect of thioredoxin on the solubility of proteinase inhibitor 2 in an E...
The document summarizes experiments aiming to test whether co-expressing thioredoxin with proteinase inhibitor 2 (PI2) enhances PI2 solubility during overexpression in E. coli. However, previous constructs designed to test this were found to be missing the PI2 gene. The project was refocused on constructing new plasmids containing PI2 and testing its co-expression with thioredoxin.
Lab Meeting 2nd Semester- Investigating genes involved in root growth respons...
1. The document summarizes an investigation into genes involved in root growth response to the polyamine cadaverine, focusing on the put2 and put5-2 genes in Arabidopsis thaliana.
2. Preliminary experiments found put5-2 to be resistant and put2 to be sensitive to cadaverine. The researcher plans to fuse PUT5 and put2 to GFP to determine their localization and introduce PUT5 into put5-2 to test for sensitivity rescue.
3. Subsequent experiments examined put2 and put5-2 sensitivity to salt stress and attempted to generate a put2/put5-2 double mutant to understand the interaction between the genes.
rprotein2
This document summarizes the expression of recombinant β-lactoglobulin (rBLG) in prokaryotic and eukaryotic cells. In Escherichia coli, rBLG was expressed with a pET26 vector and was found predominantly in a denatured form, even when expressed in the periplasm. In eukaryotic cells like COS-7 and mouse tibialis muscle, rBLG was expressed in its native conformation. The authors quantified rBLG expression using immunoassays that distinguish between native and denatured rBLG. They found higher expression levels and native folding of rBLG in eukaryotic systems compared to prokaryotic expression
Making genome edits in mammalian cells
Looking at the kind of modifications that can be made in mammalian cells, and how at Horizon moving to a haploid model system has significantly improved efficiency of both editing and validation
PCUBE--Protein Production Platform for mAb generation. Part I
1. HEK293E cells and an episomal vector were used to transiently express monoclonal antibodies in suspension culture. Transfection with polyethylenimine resulted in high and sustained protein expression over 8 days.
2. For larger scale production, adherent 293E cells were grown in a disposable packed-bed bioreactor containing 12,000 cm2 of growth surface in 500 mL volume. Protein expression lasted over a month with repeated batch culture.
3. A monoclonal antibody was expressed using this system, purified by protein A chromatography, and confirmed to be the correct protein by mass spectrometry. Immunized mouse sera recognized the recombinant protein.
Gary kerr dundee uni seminar feb 2013
Seminar delivered by Dr Gary Kerr at the University of Dundee on 13 Feb 2013 on meiotic nuclear divisions in budding yeast require PP2ACdc55-mediated antagonism of Net1 phosphorylation by Cdk
Urja Bhatt undergraduate 8th sem project ppt
1) The document discusses the importance of central pair apparatus proteins in the flagella of the unicellular alga Chlamydomonas reinhardtii. It focuses on generating mutants through RNAi to study the role of proteins like hydin that contain an adenylate kinase domain.
2) Experiments generated hydin mutants in wild type and cpc1 backgrounds through transformation. Prospective double mutants were screened through transcript analysis and phenotype observation.
3) A reactivation assay showed that the cpc1 mutant had a ten-fold decrease in speed compared to wild type, indicating central pair proteins are required for optimal motility.
4) While RNAi was unstable, the study provides a framework
Church gmod2012 pt2
This document discusses the evolution of genome references at the National Center for Biotechnology Information (NCBI). It describes how genomic data is stored and tracked in GenBank, and how reference assemblies are developed and annotated through collaborations between NCBI, other genome centers, and the research community. The goal is to provide consistent, high-quality reference genomes and annotations across multiple assemblies.
Vit plus collagen by wink
This document discusses Wink Premium Collagen, a supplement that contains collagen fibers and elastin to support skin health. It contains 10 active ingredients including collagen, elastin, stem cells, glutamine, OPC, CoQ10, pine bark extract, and growth hormones that may provide anti-aging benefits. Taking one capsule daily of Wink Premium Collagen can help support skin elasticity, hydration, and a more youthful appearance over time.
Comparative Genomics for Marker Development in Cassava
Comparative Genomics for Marker Development in CassavaInternational Institute of Tropical Agriculture
Genomics Resources 4 Crop Improvement,Ongoing Molecular Projects,Cloned and characterized virus R genes in plants,Sequence Editing and AnalysisSigma Xi
This document summarizes a student project aimed at developing a gene therapy technique using CCR5 Δ32 mutation to potentially cure HIV/AIDS. The project involves collecting a blood sample from a participant with the CCR5 Δ32 mutation, purifying the DNA, inserting it into a vector, and transducing cells to integrate the modified gene and block HIV entry. The goal is to develop an easier gene therapy method that could cure HIV by reversing the virus's effects, as was seen in the case of Timothy Ray Brown.
Delta 32[1]
This document summarizes a student research project that analyzed the frequency of the CCR5 Δ32 allele in a population in Northeastern Ohio. The students mapped primers for the CCR5 gene, developed a DNA collection and analysis protocol, and tested 50 samples from their population. Their results found that 45 samples were homozygous wild-type, 5 were heterozygous for the Δ32 mutation, and none were homozygous. They conclude that the Δ32 allele frequency in this population is 5%. Future plans include further testing at their college and using the Δ32 mutation for potential gene therapy.
cf-DNA Preservative 4 Page Flyer Aug 2016
1. Norgen Biotek's cf-DNA preservative tubes preserve cell-free DNA (cf-DNA) in whole blood samples for up to 30 days at ambient temperatures without using formaldehyde.
2. The tubes maximize plasma volume recovery of 6-7mL even after shipping and prevent hemolysis and apoptosis of blood cells that could degrade cf-DNA quality.
3. cf-DNA and genomic DNA remain stable in the preservative tubes for 30 days at room temperature and 8 days at 37°C, allowing preserved samples to be shipped or stored before downstream analysis of cf-DNA.
Analysis of Delta32
This document summarizes a student research project that analyzed the frequency of the CCR5 Delta 32 allele in a population in Northeastern Ohio. The students mapped the CCR5 gene, developed a DNA collection and analysis protocol, and tested 50 samples. Their results found that 5 out of the 50 samples were heterozygous for the Delta 32 allele, indicating a gene frequency of 5% in the population. The students propose continuing their research and exploring using the Delta 32 mutation for potential gene therapy applications.
Means of Treating Botulinum Neurotoxin Persistence
This is a presentation given to the 50th annual IBRCC meeting in Annapolis, MD in Oct 2013 by George Oyler of Synaptic Research describing new treatments for botulinum intoxication.
More Related Content
What's hot
Molecular markers and plant breeding4
This document discusses the techniques of restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR). RFLP involves using restriction enzymes to cut DNA into fragments, which are then analyzed via gel electrophoresis and probing. PCR, developed in 1985, allows for exponential amplification of specific DNA regions using oligonucleotide primers, DNA polymerase, and thermal cycling. The PCR process involves repeated cycles of template DNA denaturation, primer annealing, and fragment extension, resulting in millions of copies of the target DNA. Both RFLP and PCR are useful for analyzing DNA samples.
Recombinant Antibody Overview I - Creative Biolabs
The document discusses recombinant antibody production. It begins by describing antibody structure and function, including the five main antibody isotypes found in vivo. It then details the process of natural antibody production by B cells, including B cell development, antibody gene rearrangement, and class switching. Finally, it introduces recombinant antibody production using hybridoma technology and lists the technical advantages of Creative Biolabs' hybridoma platform for generating recombinant antibodies.
Blood2007
This document summarizes the discovery of duplicated VegfA and KDR receptor genes in zebrafish that mediate vascular development. Specifically:
- The researchers identified a duplicated zebrafish VegfA gene (VegfAb) that encodes 171- and 210-amino acid isoforms not found in the single VegfA gene.
- They also found a duplicated KDR receptor gene (Kdrb) that encodes a receptor similar to mammalian KDR.
- Knockdown experiments in zebrafish showed that both VegfAb and the duplicated KDR receptor genes play important roles in vascular development.
- Further experiments demonstrated that the VegfAb isoforms are poorly secreted compared to VegfA isoforms
Imaginal discs1
The document summarizes a study on gene expression during regeneration of Drosophila wing imaginal discs. Microarray analysis found over 1,000 genes differentially expressed between cut and intact discs in the first 24 hours after wounding, indicating a strong initial response to injury. Many genes involved in apoptosis, stress response, cytoskeletal activity and JNK signaling showed increased expression. Between 24-72 hours, expression returned toward normal levels as discs resumed activities interrupted by wounding. The study identifies signaling pathways and gene categories important for disc regeneration.
Cancer
Cancer is an uncontrolled multiplication of cells that manifests as malignant or benign tumor, impairs immune system and produces fatal intoxicants.
The effect of thioredoxin on the solubility of proteinase inhibitor 2 in an E...
The document summarizes experiments aiming to test whether co-expressing thioredoxin with proteinase inhibitor 2 (PI2) enhances PI2 solubility during overexpression in E. coli. However, previous constructs designed to test this were found to be missing the PI2 gene. The project was refocused on constructing new plasmids containing PI2 and testing its co-expression with thioredoxin.
Lab Meeting 2nd Semester- Investigating genes involved in root growth respons...
1. The document summarizes an investigation into genes involved in root growth response to the polyamine cadaverine, focusing on the put2 and put5-2 genes in Arabidopsis thaliana.
2. Preliminary experiments found put5-2 to be resistant and put2 to be sensitive to cadaverine. The researcher plans to fuse PUT5 and put2 to GFP to determine their localization and introduce PUT5 into put5-2 to test for sensitivity rescue.
3. Subsequent experiments examined put2 and put5-2 sensitivity to salt stress and attempted to generate a put2/put5-2 double mutant to understand the interaction between the genes.
rprotein2
This document summarizes the expression of recombinant β-lactoglobulin (rBLG) in prokaryotic and eukaryotic cells. In Escherichia coli, rBLG was expressed with a pET26 vector and was found predominantly in a denatured form, even when expressed in the periplasm. In eukaryotic cells like COS-7 and mouse tibialis muscle, rBLG was expressed in its native conformation. The authors quantified rBLG expression using immunoassays that distinguish between native and denatured rBLG. They found higher expression levels and native folding of rBLG in eukaryotic systems compared to prokaryotic expression
Making genome edits in mammalian cells
Looking at the kind of modifications that can be made in mammalian cells, and how at Horizon moving to a haploid model system has significantly improved efficiency of both editing and validation
PCUBE--Protein Production Platform for mAb generation. Part I
1. HEK293E cells and an episomal vector were used to transiently express monoclonal antibodies in suspension culture. Transfection with polyethylenimine resulted in high and sustained protein expression over 8 days.
2. For larger scale production, adherent 293E cells were grown in a disposable packed-bed bioreactor containing 12,000 cm2 of growth surface in 500 mL volume. Protein expression lasted over a month with repeated batch culture.
3. A monoclonal antibody was expressed using this system, purified by protein A chromatography, and confirmed to be the correct protein by mass spectrometry. Immunized mouse sera recognized the recombinant protein.
Gary kerr dundee uni seminar feb 2013
Seminar delivered by Dr Gary Kerr at the University of Dundee on 13 Feb 2013 on meiotic nuclear divisions in budding yeast require PP2ACdc55-mediated antagonism of Net1 phosphorylation by Cdk
Urja Bhatt undergraduate 8th sem project ppt
1) The document discusses the importance of central pair apparatus proteins in the flagella of the unicellular alga Chlamydomonas reinhardtii. It focuses on generating mutants through RNAi to study the role of proteins like hydin that contain an adenylate kinase domain.
2) Experiments generated hydin mutants in wild type and cpc1 backgrounds through transformation. Prospective double mutants were screened through transcript analysis and phenotype observation.
3) A reactivation assay showed that the cpc1 mutant had a ten-fold decrease in speed compared to wild type, indicating central pair proteins are required for optimal motility.
4) While RNAi was unstable, the study provides a framework
Church gmod2012 pt2
This document discusses the evolution of genome references at the National Center for Biotechnology Information (NCBI). It describes how genomic data is stored and tracked in GenBank, and how reference assemblies are developed and annotated through collaborations between NCBI, other genome centers, and the research community. The goal is to provide consistent, high-quality reference genomes and annotations across multiple assemblies.
Vit plus collagen by wink
This document discusses Wink Premium Collagen, a supplement that contains collagen fibers and elastin to support skin health. It contains 10 active ingredients including collagen, elastin, stem cells, glutamine, OPC, CoQ10, pine bark extract, and growth hormones that may provide anti-aging benefits. Taking one capsule daily of Wink Premium Collagen can help support skin elasticity, hydration, and a more youthful appearance over time.
Comparative Genomics for Marker Development in Cassava
Comparative Genomics for Marker Development in CassavaInternational Institute of Tropical Agriculture
Genomics Resources 4 Crop Improvement,Ongoing Molecular Projects,Cloned and characterized virus R genes in plants,Sequence Editing and AnalysisSigma Xi
This document summarizes a student project aimed at developing a gene therapy technique using CCR5 Δ32 mutation to potentially cure HIV/AIDS. The project involves collecting a blood sample from a participant with the CCR5 Δ32 mutation, purifying the DNA, inserting it into a vector, and transducing cells to integrate the modified gene and block HIV entry. The goal is to develop an easier gene therapy method that could cure HIV by reversing the virus's effects, as was seen in the case of Timothy Ray Brown.
Delta 32[1]
This document summarizes a student research project that analyzed the frequency of the CCR5 Δ32 allele in a population in Northeastern Ohio. The students mapped primers for the CCR5 gene, developed a DNA collection and analysis protocol, and tested 50 samples from their population. Their results found that 45 samples were homozygous wild-type, 5 were heterozygous for the Δ32 mutation, and none were homozygous. They conclude that the Δ32 allele frequency in this population is 5%. Future plans include further testing at their college and using the Δ32 mutation for potential gene therapy.
cf-DNA Preservative 4 Page Flyer Aug 2016
1. Norgen Biotek's cf-DNA preservative tubes preserve cell-free DNA (cf-DNA) in whole blood samples for up to 30 days at ambient temperatures without using formaldehyde.
2. The tubes maximize plasma volume recovery of 6-7mL even after shipping and prevent hemolysis and apoptosis of blood cells that could degrade cf-DNA quality.
3. cf-DNA and genomic DNA remain stable in the preservative tubes for 30 days at room temperature and 8 days at 37°C, allowing preserved samples to be shipped or stored before downstream analysis of cf-DNA.
Analysis of Delta32
This document summarizes a student research project that analyzed the frequency of the CCR5 Delta 32 allele in a population in Northeastern Ohio. The students mapped the CCR5 gene, developed a DNA collection and analysis protocol, and tested 50 samples. Their results found that 5 out of the 50 samples were heterozygous for the Delta 32 allele, indicating a gene frequency of 5% in the population. The students propose continuing their research and exploring using the Delta 32 mutation for potential gene therapy applications.
Means of Treating Botulinum Neurotoxin Persistence
This is a presentation given to the 50th annual IBRCC meeting in Annapolis, MD in Oct 2013 by George Oyler of Synaptic Research describing new treatments for botulinum intoxication.
What's hot (20)
Recombinant Antibody Overview I - Creative Biolabs
Recombinant Antibody Overview I - Creative Biolabs
The effect of thioredoxin on the solubility of proteinase inhibitor 2 in an E...
The effect of thioredoxin on the solubility of proteinase inhibitor 2 in an E...
Lab Meeting 2nd Semester- Investigating genes involved in root growth respons...
Lab Meeting 2nd Semester- Investigating genes involved in root growth respons...
PCUBE--Protein Production Platform for mAb generation. Part I
PCUBE--Protein Production Platform for mAb generation. Part I
Comparative Genomics for Marker Development in Cassava
Comparative Genomics for Marker Development in Cassava
Means of Treating Botulinum Neurotoxin Persistence
Means of Treating Botulinum Neurotoxin Persistence
Viewers also liked
Cara instalasi moodle 2
Cara instalasi Moodle 2.1 pada sistem operasi Windows Vista dan 7 meliputi langkah-langkah sebagai berikut: (1) download file Moodle dan XAMPP, (2) install XAMPP dan ekstrak file Moodle, (3) konfigurasi direktori dan izin akses file, (4) aktifkan Apache dan MySQL di XAMPP, (5) lanjutkan proses instalasi Moodle.
MARKA FİNANSMANI
Marka finansmanı, markaların anlaşılması, ölçülmesi, yönetilmesi ve kullanılması konusunda uzmanlaşıldan söz etmektedir.
Just what is a TeachMeet and why should I care?
A seven minute presentation, titled Just what is a TeachMeet and why should I care? prepared for the QUT 2015 National Summit:
G E P2010 Full Report
This document is a pre-publication draft of the World Bank's Global Economic Prospects report for 2010. It contains an overview that discusses how the acute phase of the global financial crisis is over but developing countries still face medium-term challenges from the effects of the boom and bust cycle. These include higher borrowing costs, potential retreat from financial integration, and impacts on supply potential. The draft is embargoed and not to be shared until its official publication date of January 21, 2010.
Marka fi̇nansmanı
Marka finansmanı, markaların anlaşılması, ölçülmesi, yönetilmesi ve kullanılması konusunda uzmanlaşılmasından bahsetmektedir.
PROJE HIZLANDIRMA
Bu yaklaşım, proje yöneticisine, bazı faaliyetlere kaynak ilave ederek, faaliyet süresini, dolayısıyla proje tamamlama süresini azaltma(hızlandırma) olanağı verir.
The Lottery
Shirley Jackson's short story "The Lottery" shocked many readers when it was published in 1948 for its portrayal of a brutal stoning ritual in a small town. Jackson had hoped the story would dramatize the pointless violence and inhumanity in ordinary lives. The story highlights the hypocrisy of a society where violence is accepted until it becomes personal, as the townspeople participate in stoning one of their own members to death in a ritual sacrifice.
Etkinlik Pazarlaması
Etkinlik pazarlaması, bir marka veya firmanın "ana/anahtar mesajları"nı, farklı metodlar kullanarak, oluşturulacak olan bir veya birkaç "Etkinlik" aracılığı ile hedef kitleye ulaştırmasıdır.
Directors Remuneration And Golden Parachutes.Ppt
A summary of recent developments in Belgium with regard to the remuneration of directors in listed companies and government owned companies, and a summary of the CBFA\'s circular on remuneration of important persons within financial institutions.
Client seminar presentation of 29 March 2010
Marka bilinirliği
Marka bilinirliği ise; Marka farkındalığına bağlı olarak gelişen ve tüketiciye markayla ilgili ipucu verildiği durumlarda, geçmiş bilgi ve deneyimlerini kontrol eden tüketicinin zihninde markaya ilişkin bilginin belirlenmesi süreci olarak tanımlanmaktadır.
Pazarlama bilgi sistemleri
proaktif pazarlama bir halkla ilişkiler ve pazarlama
faaliyetlerinin birleşimi olan Pazarlama Yönlü Halkla İlişkilerin (MPR) bir parçasıdır.
Viewers also liked (17)
Similar to Project Overview
Seminario biologia molecular
This document summarizes a medical student's research on the differential effects of the protein kinase C-eta (PKCη) on apoptosis versus senescence. The student conducted experiments using cell culture, transfection, and Western blot analysis to determine that depletion of PKCη induces senescence and upregulates the protein p27, leading to resistance to chemotherapy, while depletion of p27 can restore apoptosis. The research contributes to understanding PKCη's controversial roles in tumor promotion and suppression and could help develop more targeted chemotherapy treatments.
Avacta Life Sciences Affimers Presentation Global Protein Engineering Summit ...
Avacta Life Sciences Exhibits Affimers at Global Protein Engineering Summit
Avacta Life Sciences exhibited recently at the Global Protein Engineering Summit ("PEGS") where it presented its Affimer technology.
You can read more about Affimer technology here http://www.avactalifesciences.com
PEGS is considered to be the essential protein engineering meeting where commercial and academic progress in protein engineering is showcased and this year it attracted over 1800 delegates from across the globe to Boston. Avacta Life Sciences presented its Affimer technology for the first time at a PEGS meeting with technical exhibits and a presentation by the CSO, Paul Ko Ferrigno, entitled "Biological Recognition: Beyond the Antibody."*
The exhibition booth was busy with over 80 delegates talking to the Avacta Life Science management team over the four days of the summit. The feedback on the Affimer technology was very positive, in particular, the short development times and excellent stability were highlighted by delegates as key advantages of Affimers over antibodies. There was also a strong interest in Affimers from the management of companies developing biological therapeutics who were keen to learn more about the potential of Affimers as novel therapeutics.
In addition, several companies were interested in the use of Affimers as an alternative to antibodies in diagnostic devices, mainly because they could generate binders against new biomarkers much more quickly and evaluate them in higher numbers.
The benefits of Affimer microarrays for biomarker discovery also resonated with diagnostic developers who appreciated the advantage of being able to evaluate significantly larger numbers of potential biomarkers more cost and time effectively than by mass spectrometry. The potential of the arrays for multiplexed solutions for clinical diagnosis and monitoring during drug trials was also something that generated interest amongst those delegates.
Matt Johnson, Chief Technical Officer of Avacta Life Sciences commented: "It was great to experience face to face the level of interest in Affimers. The majority of people I spoke to were either having problems raising antibodies to their target of interest or just couldn't use antibodies because of the type of assays they wanted to perform. Many of the presentations focused around the use of antibody fragments for intra-cellular studies which is a rapidly growing area that holds great interest for drug and diagnostics developers. It is an area where there are clear advantages for Affimers over antibody fragments which don't behave well in the cytoplasm.
"The general enthusiasm around Affimers was very encouraging and the amount of interest generated by the potential of Affimers as therapeutics and by the Affimer arrays for biomarker discovery only reinforces my excitement around this new technology."
Regulation of cell cycle
Regulation of cell cycle - presented by Dr. Karthikeyan at Department of biochemistry, Maulana Azad Medical College, NewDelhi
Hepatitis C
This document discusses the analysis of protein BAB71591 which is deregulated during Hepatitis C infection. The analysis involved:
1) Retrieving the protein sequence from a database and predicting its unknown function using bioinformatics tools. It was predicted to localize to the cytoplasm and function as a methyltransferase.
2) Building a homology model based on a phospholipase template to predict the protein structure. The model showed alpha helices and beta sheets.
3) Correlating the putative functions to Hepatitis C, finding they may be downregulated during infection through methylation or inflammatory responses.
132 gene expression in atherosclerotic plaques
The document describes a microarray study to analyze gene expression in atherosclerotic plaques and correlate it with factors related to plaque vulnerability. Specimens will be obtained from human carotid/coronary arteries and atherosclerotic plaques in mouse models. Gene expression will be profiled using microarrays and correlated with histopathology, pH, temperature, spectroscopy and other variables. The goal is to identify genes associated with vulnerable plaques and rupture. Plaques from influenza-infected and drug-treated mice will also be analyzed to study effects on gene expression and plaque structure.
132 gene expression in atherosclerotic plaques
This document discusses microarray studies to analyze gene expression in atherosclerotic plaques and correlate it with factors related to plaque vulnerability. It begins with background on the history and applications of DNA microarrays. Key steps discussed include probe design, sample preparation including tissue collection, labeling RNA samples, hybridizing samples to a microarray chip, scanning and analyzing image data. The document outlines creating a custom microarray based on selected genes and correlating gene expression with temperature, pH, spectroscopy and histopathology of plaques. It will also analyze gene expression in influenza-infected mice and mice where plaques are induced to rupture with drugs. Human carotid artery specimens from surgery will be analyzed from symptomatic and asymptomatic patients.
Micro array study for gene expression in vp
The document describes a microarray study to analyze gene expression in atherosclerotic plaques and correlate it with factors related to plaque vulnerability. Specimens will be obtained from human carotid/coronary arteries and atherosclerotic plaques in mouse models. Gene expression will be profiled using microarrays and correlated with histopathology, pH, temperature, spectroscopy and other variables. Plaques from influenza-infected and drug-treated mice will also be analyzed to identify genes associated with plaque rupture. The goal is to better understand plaque vulnerability and identify potential drug targets.
Current Chemotherapy and the Future of Targeted Therapies | Mesothelioma Appl...
Current Chemotherapy and the Future of Targeted Therapies | Mesothelioma Appl...Mesothelioma Applied Research Foundation
Current Chemotherapy and the
Future of Targeted Therapies presented by Lee Krug, MD, of Memorial Sloan-Kettering Cancer Center at the Mesothelioma Applied Research Foundation's conference in New York, NY on September 28, 2012. www.curemeso.org2008 Zing Small Molecule Drug Discovery Talk
This document discusses kinase inhibitors and p38 MAP kinase as a drug target. It provides background on kinases and their role in disease. Many kinase inhibitors have been developed and some have been approved to treat cancers. The document discusses challenges with developing kinase inhibitors and strategies to increase selectivity, such as targeting inactive kinase conformations. It describes a compound called KC706 that was developed by Kemia to selectively inhibit p38α kinase in a time-dependent manner. KC706 shows potent and selective inhibition of p38α kinase activity and cytokine production in vitro.
Biochain PCR Products
The document discusses BioChain's products for PCR and sample preparation, including PCR enzymes, reverse transcriptases, master mixes, dNTPs, and supporting reagents. It provides details on BioChain's Taq DNA polymerase and Hot Start Taq DNA polymerase, which are produced under strict quality control. It also describes BioChain's UltraScript reverse transcriptase, which is ideal for cDNA synthesis of templates with secondary structure or high GC content. Furthermore, it mentions BioChain's pre-mixed master mixes for standard and quantitative PCR, which offer convenience and reproducibility.
Beta lactam Antibiotics .ppt
β-lactam antibiotics work by inhibiting bacterial cell wall synthesis. There are several classes of β-lactam antibiotics including penicillins, cephalosporins, monobactams, and carbapenems. Penicillins are derived from Penicillium fungi and contain a thiazolidine ring fused to the β-lactam ring. Cephalosporins are derived from the fungus Cephalosporium and contain a dihydrothiazine ring fused to the β-lactam ring. Both penicillins and cephalosporins target bacterial transpeptidases to inhibit cell wall crosslinking. Structural modifications to these classes of β-lactams can
Ig genetics08.ppt
The document summarizes immunoglobulin genetics and the generation of antibody diversity. It discusses how the V, D, and J gene segments rearrange through V(D)J recombination to generate a diverse repertoire of B cell receptors. Additional diversity is created through junctional diversity from nucleotide additions by the enzyme terminal deoxynucleotidyl transferase (TdT) and somatic hypermutation. Together, these mechanisms allow for over 10 billion potential antibody specificities.
summerspowerpoint2013 (2)
1) The document discusses research into expressing and purifying the CANC domains of the Gag polyprotein from murine leukemia virus (MLV). CANC consists of the capsid and nucleocapsid domains.
2) The researchers successfully expressed CANC in E. coli cells using a GST fusion system and are working to improve solubility at higher concentrations. They aim to use purified CANC in gel shift assays to study protein-RNA interactions.
3) They are also optimizing conditions for in vitro transcription of the 101 nucleotide core encapsidation signal from within the MLV packaging signal, which is important for viral genome packaging.
Purification of regucalcin from the seminal vesicular fluid
This document discusses the purification of regucalcin (RGN), a calcium-binding protein, from seminal vesicular fluid. It introduces RGN and its functions in maintaining calcium homeostasis and roles in apoptosis and antioxidant activity. The authors describe using PCR, Western blot, and SDS-PAGE techniques to demonstrate that RGN is present in seminal fluid and confirm its identity using mass spectrometry. Their results show RGN can be purified from seminal fluid using an immunoaffinity method and identify its molecular weight. The conclusion states RGN's important roles and potential applications for developing cancer treatments.
Delivering More Efficient Therapeutic Protein Expression Systems Through Cell...
Historically cell line performance has been enhanced through media, feed and process optimization, primarily through trying to meet the basic nutritional requirements of the cells so that they can sustain high growth and productivity throughout the production runs.
However, the omics (genomics, transciptomics and metabolomics) era, sequencing of the CHO genome and enhancements in genome editing technologies over the past several years have enabled scientists to take a more direct route in cell line optimization through the modification of specific genes that have direct implications on cell culture performance, protein quality attributes and upstream and downstream manufacturing processes. These targets include but are not limited to genes that may be involved in cell cycle regulation, cellular metabolism, cellular transcription and translation, the secretory pathway and protein glycosylation or other post-translational modifications.
In this webinar we will discuss specific genetic modifications that have been made to CHO cell lines and how these modifications can lead to more efficient expression systems.
CLONING VECTOR
1. The document discusses different types of cloning vectors including plasmids, bacteriophages, cosmids, and phagemids that can be used to clone foreign DNA.
2. It provides details on the characteristics and components of common vectors like the pBR322 plasmid and lambda phage. Screening methods for identifying recombinant clones like blue/white selection and replica plating are also described.
3. Applications of genetic engineering using these vectors include producing recombinant proteins like insulin and hepatitis vaccines as well as studying gene function and identifying mutations associated with diseases.
Protein protein interactions-ppt
This document discusses methods for identifying protein-protein interactions, including the yeast two-hybrid system and co-immunoprecipitation. It provides details on how each technique works and describes an example application investigating interactions in B cell receptor signaling pathways. Key proteins in the B cell receptor pathway are identified that could be used as baits in future experiments.
Delivering More Efficient Therapeutic Protein Expression Systems Through Cell...
Historically cell line performance has been enhanced through media, feed and process optimization, primarily through trying to meet the basic nutritional requirements of the cells so that they can sustain high growth and productivity throughout the production runs.
However, the omics (genomics, transciptomics and metabolomics) era, sequencing of the CHO genome and enhancements in genome editing technologies over the past several years have enabled scientists to take a more direct route in cell line optimization through the modification of specific genes that have direct implications on cell culture performance, protein quality attributes and upstream and downstream manufacturing processes. These targets include but are not limited to genes that may be involved in cell cycle regulation, cellular metabolism, cellular transcription and translation, the secretory pathway and protein glycosylation or other post-translational modifications.
In this webinar we will discuss specific genetic modifications that have been made to CHO cell lines and how these modifications can lead to more efficient expression systems.
Adipose derived osteoblasts cells from fat tissues
This study characterized and compared surface proteins of primary osteoblasts isolated from iliac crest bone and osteoblast-like cells differentiated from adipose-derived mesenchymal stem cells. Gene and protein expression analysis using RT-PCR and western blotting showed that both cell types expressed osteoblast markers like osteocalcin, alkaline phosphatase, and collagen type 1, though osteocalcin expression was lower in adipose-derived cells. The study also found expression of stem cell markers and nucleostemin in both osteoblasts and adipose-derived osteoblast-like cells.
GKA deel 1 college 11
The document discusses apoptosis, or programmed cell death. It describes how apoptosis controls cell death by removing abnormal cells. The major actors in apoptosis are caspases, which are proteases that cleave substrates once or twice. Caspases activate caspase-activated DNases, which degrade DNA and lead to cell death. There are two major apoptosis pathways, intrinsic and extrinsic, which are initiated by caspase 8 or 9 and involve death receptors or intracellular stresses.
Similar to Project Overview (20)
Avacta Life Sciences Affimers Presentation Global Protein Engineering Summit ...
Avacta Life Sciences Affimers Presentation Global Protein Engineering Summit ...
Current Chemotherapy and the Future of Targeted Therapies | Mesothelioma Appl...
Current Chemotherapy and the Future of Targeted Therapies | Mesothelioma Appl...
Purification of regucalcin from the seminal vesicular fluid
Purification of regucalcin from the seminal vesicular fluid
Delivering More Efficient Therapeutic Protein Expression Systems Through Cell...
Delivering More Efficient Therapeutic Protein Expression Systems Through Cell...
Delivering More Efficient Therapeutic Protein Expression Systems Through Cell...
Delivering More Efficient Therapeutic Protein Expression Systems Through Cell...
Adipose derived osteoblasts cells from fat tissues
Adipose derived osteoblasts cells from fat tissues
Project Overview
- 1. Analysis of the atypical PKC isoforms from sequence to function
- 2. Family of Protein Kinases C features: ~10 members - ser/thr kinases - 80kDa - 3 subfamilies based on second messenger requirements: 1. classical (DAG, Ca2+) 2. novel (DAG)
- 3. Family of Protein Kinases C
- 4. Family of Protein Kinases C • the two isoforms show a 72% homology on the amino acid level
- 5. Family of Protein Kinases C • the two isoforms show a 72% homology on the amino acid level • both share well defined domains with characterized functions • PB1, binding of other PB1 containing proteins (e.g. par-6) • C1, protein-interaction through its zinc-finger motif • kinase, phosphorylation and binding of par-3
- 6. There are known knowns. These are things we know that we know. There are known unknowns. That is to say, there are things that we know we don't know. But there are also unknown unknowns. There are things we don't know we don't know. Donald Rumsfeld
- 7. Known knows: • The aPKCι KO is lethal at embryonic stage 7.5 probably due to defects in cell polarity, while the aPKCζ KO shows a milder phenotype, reflected in impaired NfκB signaling (Leitges et al., 2001) • [...] some functions of aPKC are independent of its In drosophila: kinase activity [...] (Kim et al., 2009) • The aPKCι is required for bronchioval stem cell expansion and tumorigenesis (Regala et al., 2009) • Expression of P-aPKC-iota, E-cadherin, and beta-catenin related to invasion and metastasis in hepatocellular carcinoma. (Du et al., 2009)
- 8. Known unknowns biochemical weapons: Comparison of aPKCι antibodies:
- 9. Known unknowns biochemical weapons: Comparison of aPKCι antibodies: Company Antigen Abcam full length Stressgen full length and partial protein B&D AA 404 - 587 Novus AA 1-101 R&D AA 446 - 587 Santa Cruz AA 168 - 243
- 10. Known unknowns biochemical weapons: Comparison of aPKCι antibodies: Company Antigen Abcam full length Stressgen full length and partial protein B&D AA 404 - 587 Novus AA 1-101 R&D AA 446 - 587 Santa Cruz AA 168 - 243 specificity of antibodies is uncertain
- 11. Unknown unknowns
- 12. Strategy
- 13. Strategy
- 14. Strategy
- 15. Strategy
- 16. Strategy
- 17. Strategy
- 18. Strategy
- 19. Strategy
- 20. Strategy The deletion of the kinase domain drives aPKCι, but NOT aPKCζ, into the nucleus.
- 21. Shuttling of aPKCs • Both isoforms contain Nuclear Localization Sequences (NLS) and a Nuclear Export Sequence (NES), implicating the involvement of an importin related shuttling mechanism.