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DEL MAR COLLEGE
Department of Natural Sciences



LABORATORY SAFETY PROCEDURES & GUIDELINES
             A Pre-Lab Course



           24-Hour Emergency Number:
        DMC Security Office 361 / 698-1199
INSTRUCTIONS

 • Study the information in this module.

 • Take the quiz following this module.

 • You must score 100% on this quiz to stay
   registered in the class.
TABLE OF CONTENTS

 Topic                              Module
 General Rules and Regulations      1
 Chemical Safety                    2
 Biohazard Safety                   3
 Emergency Equipment & Procedures   4
 Field Trip Safety                  5
MODULE 3

BIOHAZARD SAFETY
BIOHAZARDS
          BIOHAZARDS INCLUDE:
                  Bacteria
                   Fungi
                  Viruses
                 Parasites
              any bodily fluids
and anything that comes in contact with them.
BLOODBORNE PATHOGENS (BBPs)
 PATHOGENS are agents that cause disease.

 BLOODBORNE PATHOGENS are microorganisms
  (bacteria, viruses) that may be transmitted through
  blood or other potentially infectious materials such as
  bodily fluids (blood, saliva, urine, etc.) or tissues.

 Diseases of concern include Hepatitis viruses, and HIV
  (AIDS virus). The possibility of contracting one of
  these diseases should be a serious concern for
  everyone. Care should be taken to prevent
  contamination.
BLOODBORNE PATHOGEN
TRANSMISSION
You can be contaminated with a bloodborne pathogen:
  • through a cut or puncture from a contaminated
    sharp object
  • when you touch a contaminated surface and then
    touch your eyes, nose, mouth, open wounds, or
    inflamed skin
CONTROLLING EXPOSURE TO BBPs
Exercise Universal Precautions!
● Treat all blood and bodily fluids as if they were known to be
  infectious (Universal Precautions).
● Avoid touching your face, eyes and nose with your gloves
● Wash hands before leaving the laboratory.

● Follow proper work practices

● Use safety equipment such as Fume Hoods

● Wear PPE as required by Laboratory Supervisor

● Follow proper waste disposal practices
BIOHAZARD DISPOSAL
• Specific waste disposal procedures depend on the type of
  waste involved.

• Follow the disposal instructions as provided by the
  Laboratory Supervisor.

• These photos are examples of containers
  that you may see in the laboratory.
BIOHAZARD DISPOSAL
Biohazard Contaminated Soft Items

Items contaminated with biohazards must
be placed in a “Biohazardous Waste”
container. These items include:

   • Disposable pipettes
   • Gloves
   • Plastic petri dishes
   • Microcentrifuge tubes, etc.




 Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSAL
Sharps Disposal

● Items identified as “Sharps” will be deposited in a rigid
  “Sharps” container. These items include:
     ● Used scalpel blades
     ● Hypodermic Needles
     ● Razor blades
    ● Contaminated glass
       slides
● Do not recap hypodermic
  needles prior to disposing
  in a “Sharps” container.


 Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSAL
Non-biohazard Items

Items NOT contaminated with biohazards and can NOT
be placed in regular trash container must be placed in
designated waste containers. These items include:
   ● Broken glass and microscope slides
   ● Autoclavable items such as:
      o Disposable pipettes
                                                             Broken
      o Plastic petri dishes                                  Glass

      o Microcentrifuge tubes, etc.


 Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSAL
Non-biohazard Dissection Items

Solid and liquid waste items generated during a
dissection must be disposed of in separate designated
waste containers.




        Liquids                                               Solids
         Only                                                  Only




 Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSAL
Laboratory Trash Items

Items NOT contaminated with biohazards can be placed
in the regular trash container. These items include:

   ● Used gloves
   ● Paper towels, etc.




 Follow the disposal instructions as provided by the Laboratory Supervisor.
REMINDER



   Please complete the quiz for
   Module 3 before continuing.

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Module3 2012

  • 1. DEL MAR COLLEGE Department of Natural Sciences LABORATORY SAFETY PROCEDURES & GUIDELINES A Pre-Lab Course 24-Hour Emergency Number: DMC Security Office 361 / 698-1199
  • 2. INSTRUCTIONS • Study the information in this module. • Take the quiz following this module. • You must score 100% on this quiz to stay registered in the class.
  • 3. TABLE OF CONTENTS Topic Module General Rules and Regulations 1 Chemical Safety 2 Biohazard Safety 3 Emergency Equipment & Procedures 4 Field Trip Safety 5
  • 5. BIOHAZARDS BIOHAZARDS INCLUDE: Bacteria Fungi Viruses Parasites any bodily fluids and anything that comes in contact with them.
  • 6. BLOODBORNE PATHOGENS (BBPs)  PATHOGENS are agents that cause disease.  BLOODBORNE PATHOGENS are microorganisms (bacteria, viruses) that may be transmitted through blood or other potentially infectious materials such as bodily fluids (blood, saliva, urine, etc.) or tissues.  Diseases of concern include Hepatitis viruses, and HIV (AIDS virus). The possibility of contracting one of these diseases should be a serious concern for everyone. Care should be taken to prevent contamination.
  • 7. BLOODBORNE PATHOGEN TRANSMISSION You can be contaminated with a bloodborne pathogen: • through a cut or puncture from a contaminated sharp object • when you touch a contaminated surface and then touch your eyes, nose, mouth, open wounds, or inflamed skin
  • 8. CONTROLLING EXPOSURE TO BBPs Exercise Universal Precautions! ● Treat all blood and bodily fluids as if they were known to be infectious (Universal Precautions). ● Avoid touching your face, eyes and nose with your gloves ● Wash hands before leaving the laboratory. ● Follow proper work practices ● Use safety equipment such as Fume Hoods ● Wear PPE as required by Laboratory Supervisor ● Follow proper waste disposal practices
  • 9. BIOHAZARD DISPOSAL • Specific waste disposal procedures depend on the type of waste involved. • Follow the disposal instructions as provided by the Laboratory Supervisor. • These photos are examples of containers that you may see in the laboratory.
  • 10. BIOHAZARD DISPOSAL Biohazard Contaminated Soft Items Items contaminated with biohazards must be placed in a “Biohazardous Waste” container. These items include: • Disposable pipettes • Gloves • Plastic petri dishes • Microcentrifuge tubes, etc. Follow the disposal instructions as provided by the Laboratory Supervisor.
  • 11. BIOHAZARD DISPOSAL Sharps Disposal ● Items identified as “Sharps” will be deposited in a rigid “Sharps” container. These items include: ● Used scalpel blades ● Hypodermic Needles ● Razor blades ● Contaminated glass slides ● Do not recap hypodermic needles prior to disposing in a “Sharps” container. Follow the disposal instructions as provided by the Laboratory Supervisor.
  • 12. BIOHAZARD DISPOSAL Non-biohazard Items Items NOT contaminated with biohazards and can NOT be placed in regular trash container must be placed in designated waste containers. These items include: ● Broken glass and microscope slides ● Autoclavable items such as: o Disposable pipettes Broken o Plastic petri dishes Glass o Microcentrifuge tubes, etc. Follow the disposal instructions as provided by the Laboratory Supervisor.
  • 13. BIOHAZARD DISPOSAL Non-biohazard Dissection Items Solid and liquid waste items generated during a dissection must be disposed of in separate designated waste containers. Liquids Solids Only Only Follow the disposal instructions as provided by the Laboratory Supervisor.
  • 14. BIOHAZARD DISPOSAL Laboratory Trash Items Items NOT contaminated with biohazards can be placed in the regular trash container. These items include: ● Used gloves ● Paper towels, etc. Follow the disposal instructions as provided by the Laboratory Supervisor.
  • 15. REMINDER Please complete the quiz for Module 3 before continuing.