The document describes the four mitotic stages: 1. Prophase, where DNA coils into chromosomes and the nucleus and nucleolus disappear. 2. Metaphase, where chromosomes align in the middle of the cell. 3. Anaphase, where sister chromatids are separated and pulled to opposite ends. 4. Telophase, where the nucleus and chromosomes reform and the cell divides into two daughter cells through cytokinesis.

2. Four Mitotic Stages Pro phase Meta phase Ana phase Telo phase

3. Prophase

4. Steps in Prophase DNA coils tightly & becomes visible as chromosomes

5. Prophase continued 2. Membrane around nucleus disappears 3. Nuceolus disappears 4. Centrioles migrate to poles 5. Spindle begins to form * the job of the spindle is to attach to the middle of each chromosome and pull the chromatids apart * (see next slide for image of spindle)

6. Mitotic Spindle

7. Eukaryotic Chromosome At the point in the center of the chromosome, labeled kinetochore, fibers attach in order to pull the two halves apart.

9. Human Chromosomes

10. Steps in Metaphase Spindle fibers from centrioles attach to each chromosome Cell preparing to separate its chromosomes Cell aligns its chromosomes in the middle of the cell Remember: meta = middle

11. Metaphase Chromosomes lined up at the equator Asters at the poles Spindle Fibers

12. Metaphase

13. Steps in Anaphase Cell chromosomes are separated Spindle fibers shorten so chromosomes pulled to ends of cell

14. Anaphase

15. Anaphase Sister chromatids being separated

16. Steps in Telophase Separation of chromosomes completed Cell Plate forms (plants) Cleavage furrow forms (animals) Nucleus & nucleolus reform Chromosomes uncoil

17. Telophase Plant Animal

18. Cytokinesis Means division of cytoplasm and all organelles within Occurs after chromosomes separate Forms two, identical daughter cells

19. Cytokinesis Cell Plate Forming in Plant Cells

20. Cytokinesis Cleavage furrow in animal cell Cell plate in animal cell

21. Stages of M phase of cell cycle

22. Put the following in the correct order: