Mise-en-scene refers to everything that appears within the frame of a film, including setting, costume, performance, positioning of characters, props, lighting, and color. These elements are used to convey meaning about characters, their relationships, and the overall story or tone. For example, an isolated setting in a horror film creates tension by limiting opportunities for help, costumes can communicate a character's wealth or state of mind, and positioning draws attention to important characters or props in a scene. Together, mise-en-scene provides crucial context and insight for the audience.