Mise-en-scene refers to everything within the frame of a shot that can be used to tell the story, including the five main elements: settings and props, costumes/makeup, facial expressions/body language, lighting/color, and character/object positioning. These elements help establish expectations, provide context about time period and culture, convey characters' feelings and relationships, and highlight important aspects through techniques like lighting, camera angles, and character placement within the frame. Mise-en-scene is a key filmmaking tool used to manipulate audiences and advance the narrative.