The 5 elements of mise-en-scene that influence audience expectations are: 1) Settings and props which establish time, place, and social status of characters. 2) Costumes, hair, and makeup which reveal character traits and time period. 3) Facial expressions and body language which clearly convey a character's emotions and thoughts. 4) Lighting and color which set mood and draw attention through techniques like shadows and high/low key lighting. 5) Camera shots and angles which manipulate space, proximity, and viewer perspective to determine importance and relationships between elements through techniques like close-ups, long shots, and camera placement.