Manual RBC, WBC platelet count sop Pathofast CBC Peripheral Smear - Normal Ranges [https://www.pathofast.com/diagnostics/reports/Complete-Blood-Count-with-Peripheral-Smear-0e5c8d] Manual RBC count Take 3.98 ml( 3980ul) of RBC diluting fluid in a test tube Mix EDTA sample well. Take 20 ul of blood into pipette. Wipe off excess blood from the tip of the pipette Add into the test tube. Charging the Improved Neubar chamber. Take 10 ul of fluid into the pipette Place a coverslip over the chamber Discharge the fluid into the chamber Make sure it doesn’t overflow or is underfilled Counting of cells Counting is done under high power The number of cells in the RBC counting chamber is counted Calculations: No of cells/ul=no of cells counted dilution x depthx area RBCs /ul=no.of cellsx10000 Manual WBC count Take 0.38 ml( 380ul) of WBC diluting fluid Add 20 ul of whole blood Allow to stand for 10 min Load on Neubars chamber as described before Wbc count calculate no. of cells in all 4 large squares (depth=1/10 mm dilution=1:20) No. of WBCs= no. of cells counted in 4 chambersx50 Ex: no. of cells counted in 4 wbc chambers is 150 then wbc count is 150x50=7500 Manual Platelet Count Take 1.98 ml of platelet diluting fluid Add 20 ul of blood Allow to stand for 5 min Charge the neubars chamber as described before Count all platelets in 4 large wbc squares Platelet/ul =no. of platelets in 4 WBC squaresx10x2.0x1/ 4x1x0.02 Or count platelets in 25 small RBC squares x1000 Charging the Improved Neubar chamber. Take 10 ul of fluid into the pipette Place a coverslip over the chamber Discharge the fluid into the chamber Make sure it doesn’t overflow or is underfilled depth=1/10 dilution= 1:10 Reference Handbook of medical laboratory technology. Robert Carman. Christian Medical Association of India. Reprint 2013.

1. Manual RBC, WBC platelet count
sop
Pathofast CBC Peripheral Smear -
Normal Ranges

2. Manual RBC count
• Take 3.98 ml( 3980ul) of RBC diluting fluid in a
test tube
• Mix EDTA sample well. Take 20 ul of blood
into pipette. Wipe off excess blood from the
tip of the pipette
• Add into the test tube.

3. • Charging the Improved Neubar chamber.
• Take 10 ul of fluid into the pipette
• Place a coverslip over the chamber
• Discharge the fluid into the chamber
• Make sure it doesn’t overflow or is underfilled

4. Counting of cells
• Counting is done under high power
• The number of cells in the RBC counting chamber is
counted
• Calculations:
• No of cells/ul=no of cells counted
• dilution x depthx area
• RBCs /ul=no.of cellsx10000

5. Manual WBC count
• Take 0.38 ml( 380ul) of WBC diluting fluid
• Add 20 ul of whole blood
• Allow to stand for 10 min
• Load on Neubars chamber as described before

6. Wbc count
• calculate no. of cells in all 4 large
squares
(depth=1/10 mm
dilution=1:20)
No. of WBCs= no. of cells
counted in 4 chambersx50
Ex: no. of cells counted in 4 wbc
chambers is 150
then wbc count is 150x50=7500

7. Manual Platelet Count
• Take 1.98 ml of platelet diluting fluid
• Add 20 ul of blood
• Allow to stand for 5 min
• Charge the neubars chamber as described
before

8. • Count all platelets in 4 large wbc squares
• Platelet/ul
=no. of platelets in 4 WBC squaresx10x2.0x1/
4x1x0.02
Or count platelets in 25 small RBC squares
x1000

9. • Charging the Improved Neubar chamber.
• Take 10 ul of fluid into the pipette
• Place a coverslip over the chamber
• Discharge the fluid into the chamber
• Make sure it doesn’t overflow or is underfilled

10. depth=1/10
dilution= 1:10

11. • Reference
• Handbook of medical laboratory technology.
Robert Carman. Christian Medical Association
of India. Reprint 2013.