Lipid-mediated lipofection is a technique for injecting genetic material into cells using liposomes, which fuse with cell membranes. It offers high transfection efficiency, works across various eukaryotic cells, and is simple to use, although it has lower efficiency in suspension cells and is dependent on cell division and endocytosis rates. Traditional methods of DNA delivery face issues like low efficiency, poor reproducibility, cell toxicity, and inconvenience.

1. Muhammad Bilal Yusuf
Student of Bs-Biotechnology at International Islamic
University Islamabad, Pakistan.
Email: bilalyusuf767@gmail.com

2. Lipid Mediated Lipofection

3. Objectives
 Lipid Mediated Lipofection ????
 Principle
 Advantages
 Disadvantages

4. Lipid Mediated Lipofection
 Lipofection (or liposome transfection) is
a technique used to inject genetic
material into a cell by means
of liposomes.
 which are vesicles that can easily merge
with the cell membrane since they are
both made of a phospholipid bilayer.

5.  Lipofection generally uses a positively charged
(cationic) lipid to form a structure with the
negatively charged (anionic) genetic material.
 Fusion of the liposome/nucleic acid transfection
complex with the negatively charged cell
membrane takes place.
 The transfection complex is thought to enter the
cell through endocytosis.
 Once inside the cell, the complex must escape the
endosomal pathway, diffuse through the
cytoplasm, and enter the nucleus for gene
expression.
Principle

7. Advantages
 Yields high transfection efficiencies.
 Works in a wide variety of eukaryotic cells.
 Is simple to perform.
 Ensures consistently reproducible results.
 Moreover, a number of cell lines normally
resistant to transfection by other methods
transfect successfully with cationic lipid
reagents.

8. Disadvantages
 Quite low transfection efficiency in
suspension cells.
 Dependence on cell division.
 As well as on high rate of endocytosis.
 Not Applicable to all cell types.

9. Problems with traditional
methods
 Methods like calcium phosphate co-
precipitation, DEAE-dextran,
polybrene, and electroporation include
problems such as:
 low efficiency of DNA delivery
 poor reproducibility
 cell toxicity
 inconvenience