Dolly the sheep was the first mammal to be cloned from an adult somatic cell, demonstrating that differentiated cells could be reprogrammed to pluripotency. Prior to Dolly, all cloning had involved embryonic cells or cells that had not yet differentiated. Dolly showed that the cell specialization process could be reversed, proving the possibility of therapeutic cloning and stem cell research using somatic cell nuclear transfer techniques. Her successful cloning from an adult cell was a major scientific breakthrough.
Cot curve dispersed repeated DNA or interspersed repeated DNA tandem repeated DNA Long interspersed repeat sequences (LINEs) Short interspersed nuclear elements (SINEs) satellite, minisatellite and microsatellite DNA Variable Number Tandem Repeat (or VNTR)
Cot curve dispersed repeated DNA or interspersed repeated DNA tandem repeated DNA Long interspersed repeat sequences (LINEs) Short interspersed nuclear elements (SINEs) satellite, minisatellite and microsatellite DNA Variable Number Tandem Repeat (or VNTR)
Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes
DNA barcoding is a technique in which species identification is performed by using DNA sequences from a small fragment of the genome, with the aim of contributing to a wide range of ecological and conservation studies in which traditional taxonomic identification is not practical.
RNA- A polymer of ribonucleotides, is a single stranded structure. There are three major types of RNA- m RNA,t RNA and r RNA. Besides that there are small nuclear,micro RNAs, small interfering and heterogeneous RNAs. Each of them has a specific structure and performs a specific function.
Molecular biology is a branch of science concerning biological activity at the molecular level.
The field of molecular biology overlaps with biology and chemistry and in particular, genetics and biochemistry.
A key area of molecular biology concerns understanding how various cellular systems interact in terms of the way DNA, RNA and protein synthesis function.
Molecular biology is the study of molecular underpinnings of the process of replication, transcription and translation of the genetic material.
Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes Eukaryotic and Prokaryotic Chromosomes
DNA barcoding is a technique in which species identification is performed by using DNA sequences from a small fragment of the genome, with the aim of contributing to a wide range of ecological and conservation studies in which traditional taxonomic identification is not practical.
RNA- A polymer of ribonucleotides, is a single stranded structure. There are three major types of RNA- m RNA,t RNA and r RNA. Besides that there are small nuclear,micro RNAs, small interfering and heterogeneous RNAs. Each of them has a specific structure and performs a specific function.
Molecular biology is a branch of science concerning biological activity at the molecular level.
The field of molecular biology overlaps with biology and chemistry and in particular, genetics and biochemistry.
A key area of molecular biology concerns understanding how various cellular systems interact in terms of the way DNA, RNA and protein synthesis function.
Molecular biology is the study of molecular underpinnings of the process of replication, transcription and translation of the genetic material.
A short introductory presentation on Yeast Bioinformatics, focussing on the Yeast Genome and its future applications. Intended as a starting material to learn more about Saccharomyces Genomics.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
2. Cells Can Be Powered by a Variety of Free Energy Sources
Phototrophic – organisms that obtain their energy from harvesting the energy
from sunlight (e.g., many types of bacteria, plants and algae).
•We and virtually all living things that we ordinarily see around us depend
on this major input of free energy.
3. Cells Can Be Powered by a Variety of Free Energy Sources
Organotrophic – organisms that obtain their energy from feeding on other living
things (e.g., animals, fungi, gut microflora).
4. Some Cells Fix Nitrogen and Carbon Dioxide for Others
Lithotrophic - 'lithos' (rock) and 'troph' (consumer) - an organism that uses
inorganic substrates to obtain reducing equivalents for use in biosynthesis
(e.g., carbon dioxide fixation) or energy conservation via aerobic or anaerobic
respiration.
Diazotrophs are bacteria that fix Methanogens are archaea that
atmospheric nitrogen gas into a more produce methane as a metabolic
usable form such as ammonia. byproduct in anoxic conditions.
5. The Greatest Biochemical Diversity Exists Among Prokaryotic Cells
Thiomargarita namibiensis (0.75 mm)
Epulopiscium
fishelsoni (0.6 mm)
6. In extending their capacity to live in
biochemically diverse
habitats, eukaryotes went down the
path of symbiosis, rather than
reinventing the “metabolic wheel”.
7.
8.
9. In fact, the origin of eukaryotes is thought to arise
from the merging of symbiotic prokaryotic cells!
10.
11. The Tree of Life Has Three Primary Branches:
Bacteria, Archaea, and Eukaryotes
12. Ribosomal Genes
•rRNA is the most conserved (least variable) gene in all cells.
•For this reason, genes that encode the rRNA (rDNA) are
sequenced to identify an organism's taxonomic
group, calculate related groups, and estimate rates of species
divergence.
•Many thousands of rRNA sequences are known and stored
in specialized databases such as RDP-II and the European
SSU database.
13. Prokaryotes have 70S ribosomes, each consisting of a small (30S) and a large
(50S) subunit. Their large subunit is composed of a 5S rRNA subunit (consisting of 120
nucleotides), a 23S rRNA subunit (2900 nucleotides) and 34 proteins. The 30S subunit
has a 1540 nucleotide RNA subunit (16S rRNA) bound to 21 proteins.
Eukaryotes have 80S ribosomes, each consisting of a small (40S) and large (60S)
subunit. Their large subunit is composed of a 5S RNA (120 nucleotides), a 28S RNA
(4700 nucleotides), a 5.8S subunit (160 nucleotides) and ~49 proteins. The 40S
subunit has a 1900 nucleotide (18S rRNA) RNA and ~33 proteins.
14. Both prokaryotic and eukaryotic ribosomes can be broken down into two subunits
Type Size Large subunit Small subunit
prokaryotic 70S 50S (5S, 23S) 30S (16S)
eukaryotic 80S 60S (5S, 5.8S, 28S) 40S (18S)
15. The ribosomes found in mitochondria of eukaryotes also consist
of large and small subunits bound together with proteins into
one 70S particle.
These organelles are believed to be descendants of bacteria
and as such their ribosomes are similar to those of bacteria
(e.g., they have a 12S and 16S rRNA subunit)
16.
17. Chapter 1
Model organisms are those with a wealth of biological data that make them
attractive to study as examples for other species – including humans – that are
more difficult to study directly.
•Often chosen because they are easy to manipulate experimentally. This usually
will include characteristics such as short life-cycle, techniques for genetic
manipulation (inbred strains, stem cell lines, and methods of transformation) and
ease of care. They also consider size, accessibility, conservation of
mechanisms, and potential economic benefit.
•Sometimes, the genome arrangement facilitates the sequencing of the model
organism's genome, for example, by being very small or concise (e.g.
yeast, Arabidopsis, or pufferfish).
18. Genetic models have short generation times, such as the fruit fly (D.
melanogaster) and nematode worm (C. elegans) and thus, we can
study genes, etc., easier in them.
Experimental models (S. cerevisiae and E.coli) are easily manipulated
and observable traits have been documented.
Genomic models, with a pivotal position in the evolutionary tree.
Historically, model organisms include a handful of species with
extensive genomic research data, such as the NIH model organisms.
Yet, as comparative molecular biology has become more
common, some researchers have sought model organisms from a
wider assortment of lineages on the tree of life.
19. Organism Genome Sequenced Homologous Recombination Biochemistry
Prokaryote
Escherichia coli Yes Yes Excellent
Eukaryote, unicellular
Dictyostelium discoideum Yes Yes Excellent
Saccharomyces cerevisiae Yes Yes Good
Schizosaccharomyces pombe Yes Yes Good
Chlamydomonas reinhardtii Yes No Good
Tetrahymena thermophila Yes Yes Good
Eukaryote, multicellular
Caenorhabditis elegans Yes Difficult Not so good
Drosophila melanogaster Yes Difficult Good
Arabidopsis thaliana Yes No Poor
Vertebrate
Danio rerio Yes Difficult? Good
Mus musculus Yes Yes Good
Homo sapiens Yes Yes Good
21. E. coli is by far the most frequently used model organism because of its small
size, short generation time, ease of culture, and amenity to genetic manipulation.
Cultivated strains (e.g. E. coli K12) are well-adapted to the laboratory
environment, and, unlike wild type strains, have lost their ability to thrive in the
intestine. They have also been “removed” of their toxins.
Genetic information is easily transferred to E. coli, and thus we use it to amplify
DNA and express proteins (insulin). But it lacks post-translational modifications and
has codon issues, etc., most of which can be overcome.
23. Civilization owes yeast for its use since ancient times in
baking and brewing.
It is one of the most intensively studied eukaryotic model
organisms in molecular and cell biology.
Many proteins important in human biology were first
discovered by studying their homologs in yeast.
•cell cycle proteins, signaling proteins, and protein-
processing enzymes.
As a eukaryote, S. cerevisiae shares the complex internal
cell structure of plants and animals without the high
percentage of non-coding DNA that can confound research
in higher eukaryotes.
25. One of the most commonly used model organisms in
biology, including studies in genetics, physiology and life
history evolution.
•They are small and easily raised.
•Morphology is easy to identify.
•Has a short generation time (~10 days at RT)
•Has a high fecundity
•Males and females are readily distinguished (virgins)
•It has only four pairs of chromosomes.
•Genetic transformation techniques have been available
since 1987.
•About 75% of known human disease genes have a
recognizable match in the genetic code of fruit flies
27. Because a zebrafish embryo is completely transparent…
…it is widely used to study morphological development of vertebrates.
28. The mouse is one of the major model organisms for medicine
29. Mice are by far the most genetically
altered laboratory mammal.
They are the primary model organism
for most human diseases, including
cancer, because almost every human
gene has a mouse homolog.
Knock-out mice make this even more
important.
32. The Nobel Prize in Physiology or Medicine, 2007
Mario R. Capecchi, Martin J. Evans and Oliver Smithies
for their discoveries of "principles for introducing specific gene
modifications in mice by the use of embryonic stem cells"
M. Capecchi Sir M. Evans O. Smithies
Univ. of Utah Cardiff Univ., UK UNC Chapel Hill
33. Go to this website to perform your gel electrophoresis
http://learn.genetics.utah.edu/content/labs/gel/
Once you understand the process, use your DNA detective
skills to help solve a mystery.
http://www.pbs.org/wgbh/nova/sheppard/analyze.html
Or google NOVA DNA Fingerprint
NOVA Online | Killer's Trail | Create a DNA Fingerprint
34. Plasmid - circular DNA molecule found in
bacteria
genetic marker - gene that makes it possible
to distinguish organisms that carry a
plasmid with foreign DNA from those that
don’t
Recombinant DNA – DNA that has been
created artificially. DNA from two or more
sources is incorporated into a single
recombinant molecule.
37. Transforming Animal Cells
• Can be
transformed
similar to plants.
• Some eggs are
large enough to
physically inject
new DNA by
hand. Which
can “Knock Out”
a gene
38. Why?
1. Study gene function and regulation
2. Generate new organismic tools for other
fields of research.
3. Cure genetic diseases.
4. Improve agriculture and related raw materials.
5. Generate new systems or sources for
bioengineered drugs (e.g., use plants
instead of animals or bacteria).
39. term used to refer to an organism that contains genes
from other organisms
40. Transgenic Transgenic Transgenic
Bacteria Plants animals
Produce Stronger plants More
clotting factors More production
insulin production
HGH Pest resistance
41. The organism of choice for mammalian
genetic engineers.
- small
- hardy
- short life cycle
- genetics possible
- many useful strains and tools
42.
43. Vector with a transgene
tk1 & tk2 - Herpes Simplex Virus thymidine kinase genes
(make cells susceptible to gancyclovir)
Neo - neomycin resistance gene
Homologous regions - homologous to the chromosomal target
Transgene - foreign gene
44. Example of what happens with N-H recombination
Nonhomologous r e combination
homolo gous homolo gous
tk 1 se quence neo tr ansge ne se quence tk 2
chr omo some
homolo gous homolo gous
tk 1 se quence tr ansge ne se quence tk 2
neo
chr omo some
Transformed cells are neo-resistant, but gancyclovir sensitive.
homol-->
45. What happens with HR
Homologous re combinants
homolo gous homolo gous
tk 1 se quence neo tr ansge ne se quence tk 2
chr omo some
homolo gous homolo gous
se quence neo tr ansge ne se quence
chr omo some
If DNA goes in by HR, transformed cells are both neo-resistant and
gancyclovir-resistant!
Use double-selection to get only those cells with a homologous
integration event.
46. To knock-out a
gene: 1.
1. Insert neo gene
into the target
KO
gene.
2. Transform KO
plasmid into
embryonic stem
cells.
3. Perform double-
selection to get
cells with the
homologous KO 2,3.
integration (neo &
gangcyclovir
resistant).
4. Inject cells with
the knocked-out
gene into a
blastocyst.
47. How to make a transgenic mouse.
Transfe ction
With DNA
Embryonic
Stem
ce lls
Blasto cyst (ES ce lls)
(mouse)
Grow in culture .
Select for those that carry the transgene.
Inject into a blastocyst
48. Inject into a blastocyst
Implant int o
pseudo pregn an t
mou se
Chimeric mouse
Ident if y of f spring w hich
carry t he t ransgene in
t heir germline.
49. (a) If the recipient stem cells are from a brown mouse, and the
transgenic cells are injected into a black (female) mouse, chimeras are
easily identified by their Brown/Black phenotype.
(b) To get a completely transgenic KO mouse (where all cells have KO
gene), mate the chimera with a black mouse. Some of the progeny will
be brown (its dominant), because some of the germ line cells will be
from the KO cells. ½ the brown mice will have the transgene
KO, because the paternal germ-line cell was probably heterozygous.
(c) To get a homozygous KO mouse (both chromosomes have the KO
transgene), cross two brown transgenic heterozygotes. ~1/4 will be
homozygous at the transgene locus.
51. member of a population of genetically
identical organisms produced from a single
cell
52. “Dolly” was an important
break through not just
because she was a
mammal.
Frogs were cloned back in
1950’s
Why was dolly so special?
Research and answer this
question for me.
Editor's Notes
First KO mice using their ES cell technology were produced in 1989 – now its like an industry