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Eric C.Y., LEE
   2011.Jan.04

eric@nucleoti.de
Why this paper?
Sequencing Technology

      Sampling     Amplification    Sequencing

                     Bacterial       Separation
1st   DNA Sample
                    Colonization    or Synthetic




2nd   DNA Sample       PCR           Synthetic
Labeling
Nanopore by IBM
Oxford Nanopore Technology

 Enzyme
Tunneling Detector and
      Nanopore
Si3N4
•   High strength over a wide temperature range

•   High fracture toughness

•   High hardness

•   Outstanding wear resistance, both impingement
    and frictional modes

•   Good thermal shock resistance

•   Good chemical resistance
STM Picture
Why minutes?
• The tunneling data are recorded at a rate
  of 5kHz.
       300,000,000/5,000=60,000 sec
            = 1000 min ~ 16hr
• In further studies, we intend to modify the
  electronics to perform tunneling
  experiments at the same acquisition rate as
  that of the ionic current (50 kHz).
               100min ~1.6hr
Comparison




         Reference:   (2010)
Conclusion

• The successful depends on the chip
  process.
• In general temperature and environment,
  it’s work.
• More close to personalized medicine.
Thanks for your attention!

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Introduction to 3rd sequencing

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