Cryo Tem Poster
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The document discusses using cryo-transmission electron microscopy (cryo-TEM) to image Paclitaxel-loaded Poly (Glutamyl – Glutamate) (PGGP) molecules to validate multi-scale computer models. PGGP samples are mounted on specialized grids, frozen to preserve their native state, and imaged using cryo-TEM to compare the real structures to predicted models. Cryo-TEM allows imaging particles like PGGP in a native environment at cryogenic temperatures to minimize structural changes and obtain high resolution images for validation.
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Cryo electron microscopy
Cryo-electron microscopy is a technique that uses electron microscopy to image samples at cryogenic temperatures, typically around -180°C. This allows the sample to be imaged in its native, frozen-hydrated state without fixatives or stains. The process involves rapidly freezing the sample so that water forms an amorphous, vitrified ice rather than crystalline ice. Images are then taken of the frozen sample and computational techniques are used to combine multiple 2D images into a 3D reconstruction of the sample. Cryo-EM is useful for determining the 3D structures of large biomolecules like proteins, viruses, and macromolecular complexes. The technique continues to advance with improvements to electron microscopes, detectors, and computational
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Cryo-electron microscopy is a technique that images biological samples at cryogenic temperatures in their native frozen hydrated state. It involves rapidly freezing samples to vitrify water and prevent ice crystal formation, then imaging them using transmission electron microscopy. This allows for 3D reconstruction of cellular structures at high resolution without chemical fixation or staining. Key advantages are maintaining the native state of samples and enabling automated 3D structure determination from 2D images. Limitations include the need for specialized equipment and sample preparation.
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1. pdf file on Termmate: https://www.termmate.com/rabia.aziz
2. YouTube: https://www.youtube.com/channel/UCKxWnNdskGHnZFS0h1QRTEA
3. Facebook: https://web.facebook.com/Chemist.Rabia.Aziz/
4. Blogger: https://chemistry-academy.blogspot.com/
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wo-photon polymerization has recently emerged as a promising technique to fabricate scaffolds for three-dimensional (3D) cell culture and tissue engineering. Here, we combined 3D-printed microscale scaffolds fabricated using two-photon polymerization with a bioactive layer-by-layer film coating. This bioactive coating consists of hyaluronic acid and poly(l-lysine) of controlled stiffness, loaded with fibronectin and bone morphogenic proteins 2 and 4 (BMP2 and BMP4) as matrix-bound proteins. Planar films were prepared using a liquid handling robot directly in 96-well plates to perform high-content studies of cellular processes, especially cell adhesion, proliferation, and BMP-induced signaling. The behaviors of two human pancreatic cell lines PANC1 (immortalized) and PAN092 (patient-derived cell line) were systematically compared and revealed important context-specific cell responses, notably in response to film stiffness and matrix-bound BMPs (bBMPs). Fibronectin significantly increased cell adhesion, spreading, and proliferation for both cell types on soft and stiff films; BMP2 increased cell adhesion and inhibited proliferation of PANC1 cells and PAN092 on soft films. BMP4 enhanced cell adhesion and proliferation of PANC1 and showed a bipolar effect on PAN092. Importantly, PANC1 exhibited a strong dose-dependent BMP response, notably for bBMP2, while PAN092 was insensitive to BMPs. Finally, we proved that it is possible to combine a microscale 3D Ormocomp scaffold fabricated using the two-photon polymerization technique with the bioactive film coating to form a microscale tumor tissue and mimic the early stages of metastatic cancer.
2014 Acta Biomaterialia
This document describes a method for fabricating porous poly(DL-lactic-co-glycolic acid) (PLGA) microspheres that fuse together at body temperature to form solid porous scaffolds, creating an injectable scaffold system. The microspheres were treated with ethanolic sodium hydroxide to increase surface porosity without disintegrating. When mixed with media and incubated at 37°C, the microspheres fused to form scaffolds with compressive strength of 0.9 MPa, porosity of 81.6%, and pore diameter of 54 μm, supporting NIH-3T3 cell attachment and proliferation in vitro. This study demonstrates a technique for producing an injectable and porous PLGA scaffold that solid
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1. The research is on a stable solution-processed polymer semiconductor with record high-mobility for printed transistors.
2. Various characterization techniques are discussed for analyzing the active layer in organic solar cells including X-ray, neutron, and scanning probe microscopy methods.
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Opv radiation
The document discusses the origin of radiation-induced degradation in polymer solar cells. It finds that charge accumulation at the interface is the primary reason for degradation, affected by the donor-acceptor mixing ratio in the bulk heterojunction. In situ measurements of polymer solar cell performance and recombination lifetimes under X-ray radiation show that devices with high acceptor concentrations experience a significant decrease in open-circuit voltage and fill factor due to radiation, while devices with low acceptor concentrations are more resistant to these changes. The findings provide a quantitative understanding and physical model of the degradation mechanism.
A04 06 0108
The document summarizes recent research on new electro-optic properties of polysiloxane liquid crystal polymers. A series of polysiloxane-based side-chain liquid crystal polymers were prepared with systematic variation in spacer length between the mesogenic units and the polymer backbone. Electro-optic measurements showed that threshold voltage decreased with increasing spacer length. This was attributed to changes in intrinsic curvature elasticity rather than orientational order. Threshold voltage also decreased with increasing temperature and orientational order parameter. The results were consistent with existing models of curvature deformation in liquid crystals and provided insight into the effects of constraints from coupling mesogenic units to the polymer backbone.
A04 06 0108
This document summarizes recent developments in side chain liquid crystal polymers, focusing on polysiloxane based polymers. It discusses how a series of these polymers were prepared with systematic variation in the spacer length between the mesogenic units and the polymer backbone. Electro-optic measurements found that increasing the spacer length lowered the threshold voltage for molecular reorientation. A simple model indicates this is due to changes in intrinsic curvature elasticity rather than orientational order as the constraints from the polymer backbone are reduced with longer spacers.
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Group4.plasma surface modification of polylactic acid to promote
Plasma surface modification of polylactic acid (PLA) films was investigated using a dielectric barrier discharge (DBD) operating at medium pressure in different atmospheres. After plasma treatment, water contact angle measurements showed increased hydrophilicity of the PLA surface. X-ray photoelectron spectroscopy revealed an increased oxygen content on treated surfaces. Cell culture tests found that plasma treatment improved initial fibroblast cell attachment and morphology compared to untreated PLA, though no difference in proliferation was observed after 7 days. Plasma treatment in air was determined to be the most economical option for modifying PLA surfaces to enhance cell interactions.
Progress in all inorganic perovskite solar cell
Since their first introduction in the research arena, the hybrid organic-inorganic perovskite photovoltaic cells have been showing frequent record breaking power conversion efficiencies (PCEs). Despite the rapid increase in PCE by engaging new perovskite materials as active layers as well as new fabrication techniques, their stability remains too poor to go for a mass production. Mainly the organic materials in the hybrid PSCs are responsible for this instability. Consequently, very recently, different approaches are taken to replace these organic components by inorganic ones to fabricate all-inorganic PSCs. Though these first-generation all-inorganic PSCs are yet to produce competitive PCEs like their counterparts, they have already demonstrated superb stability to be a propitious bidder for solar cell energy yielding. The state-of-the-art quantum dots based cells shown efficiency as high as 10.77% and intact stability for months.
EFFECT OF NANO SiO2 ON SOME MECHANICAL PROPERTIES OF BIODEGRADABLE POLYLACTIC...
Effect of nano SiO2(13.69nm)with different weight percentage (1, 3, 5wt %)on some mechanical properties of polylactic acid (PLA) is investigated .PLA film with thickness 100µm was
prepared by solution casting method .Chemical and crystal structure of PLA and its composites with 5% nano SiO2 are characterized by FTIR and X-ray diffraction techniques .
studies of new materials based on hydrazine for solar cell application USING ...
This document provides a template for a quad chart, which is used to summarize projects. It includes sections for introduction/motivation, graphical representation/approach, achievements, and a schedule comparing planned vs achieved milestones. The blank template is accompanied by an example quad chart for a project on smart solar energy harvesting, storage and utilization. The example chart summarizes the project's subtasks and achievements to date, including developing various thin film photovoltaic cells and modules with high efficiencies, establishing equipment and characterization platforms, and synthesizing new materials. It provides the project's 5-year objectives and compares progress against planned milestones.
F110146148 Laila- Ultra-thin and high transparent Cu2ZnSnSe4 - NiOx double-la...
This document summarizes a study on using a double-layered inorganic hole-transporting layer (HTL) of ultra-thin Cu2ZnSnSe4 (CZTSe) and NiOx for inverted perovskite solar cells. The CZTSe/NiOx HTL was found to enhance hole extraction, suppress electron transport, and improve stability compared to using NiOx alone. Specifically, a device with a 600°C annealed CZTSe/NiOx HTL achieved a power conversion efficiency of 13.46% and retained 73% of its initial efficiency after 350 hours of testing. The double-layer HTL was shown to reduce interface hole recombination and prevent corrosion of the
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- 1. - Akshay Chaudhari April 15 th 2009
- 11. ( Gatan 70 Cryo transfer holder, workstation and cover, cryo gloves. Pre cool the workstation along with any tools that might be used in the process. The frozen grids are transferred to the workstation using pre cooled tweezers. Attach the cryo holder dewar to the workstation. This is will constantly keeping the grids at liquid nitrogen temperature.
- 12. Fill the dewar with liquid nitrogen while being careful not to spill it or bring it around any inflammable materials Transfer the workstation and cryo holder to the EM. Top off the anti contaminator to minimize contamination of the vicinity of the sample
Editor's Notes
- There’s not much contrast between the pink font and grey background - perhaps make the background a bit darker? Get rid of the period after the ‘2009’ - this is not a sentence