This document describes various instruments used in biochemistry laboratories, including pipettes, water baths, incubators, centrifuges, colorimeters, flame photometers, pH meters, electrophoresis equipment, and chromatography. It explains the basic principles and applications of each instrument for tasks like measuring volumes, providing constant temperatures, separating particles, quantifying analyte concentrations, determining ion levels, measuring pH, separating proteins and other charged molecules, and separating mixtures.
A spectrophotometer is an instrument containing a monochromator, a device which produces a light beam containing wavelengths in a narrow band around a selected wavelength, and a means of measuring the ratio of that beam's intensity as it enters and leaves a cuvette 99 This describes a single-beam photometer.
The all the content in this profile is completed by the teachers, students as well as other health care peoples.
thank you, all the respected peoples, for giving the information to complete this presentation.
this information is free to use by anyone.
A spectrophotometer is an instrument containing a monochromator, a device which produces a light beam containing wavelengths in a narrow band around a selected wavelength, and a means of measuring the ratio of that beam's intensity as it enters and leaves a cuvette 99 This describes a single-beam photometer.
The all the content in this profile is completed by the teachers, students as well as other health care peoples.
thank you, all the respected peoples, for giving the information to complete this presentation.
this information is free to use by anyone.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
This content is suitable for medical technologists/technicians/lab assistants/scientists writing the SMLTSA board exam. The content is also suitable for biomedical technology students and people also interested in learning about test methodologies used in medical technology. This chapter describes test methodologies and their uses. Please note that these notes are a collection I used to study for my board exam and train others who got distinctions using these.
Disclaimer: Credit goes to those who wrote the notes and the examiners of each exam question. Please use only as a reference guide and use your prescribed textbook for the latest and most accurate notes and ranges. The material here is not referenced as it is a collection of pieces of study notes from multiple people, and thus will not be held viable for any misinterpretations. Please use at your own discretion.
Complete detail about the Radiopharmaceutical, General Introduction, Radioactive substance, Radioactive rays like alpha, beta and gamma rays. All the Measurement method to determine the radioactivity of any element and widely used instrument Geiger Muller Counter. And some Radiopharmaceutical product used in many diagnosis , treatment such like sodium iodide solution & capsule, Rose Bengal I 131 and Application of Radiopharmaceuticals.
Gas chromatography- “It is a process of separating component(s) from the given crude drug by using a gaseous mobile phase.”
Principle- The principle of separation in GC is “partition.”
The mixture of components to be separated is converted to vapor and mixed with the gaseous mobile phase.
The component which is more soluble in the stationary phase travels slower and eluted later.
The component which is less soluble in the stationary phase travels faster and eluted out first.
No two components have the same partition coefficient conditions.
So the components are separated according to their partition coefficient.
The partition coefficient is “the ratio of solubility of a substance distributed between two immiscible liquids at a constant temperature.’
It involves a sample being vaporized and injected onto the head of the chromatographic column.
The sample is transported through the column by the flow of inert, gaseous mobile phase.
The column itself contains a liquid stationary phase which is adsorbed onto the surface of an inert solid.
Two major types:
1. gas-solid chromatography: Here, the mobile phase is a gas while the stationary phase is a solid.
Used for separation of low molecular gases,
e.g., air components, H2S, CS2, CO2, rare gases, CO, and oxides of nitrogen.
2.Gas-liquid chromatography: The mobile phase is a gas while the stationary phase is a liquid retained on the surface as an inert solid by adsorption or chemical bonding.
Advantages-
Both qualitative and quantitative analyses are possible.
The instrument is simple, time of analysis is short.
High sensitivity.
The method is applicable to about 60% of organic compounds.
Very small sample sizes can be used.
Analysis can be highly accurate and precise.
Applications-
Quality control and analysis of drug products like antibiotics (penicillin), antivirals (amantadine), general anesthetics (chloroform, ether), sedatives/hypnotics (barbiturates), etc.
Assay of drugs – purity of a compound can be determined for drugs like :
Atropine sulfate
Clove oil
Stearic acid
In determining the levels of metabolites in body fluids like plasma, serum, urine, etc
Estimation of spoilage components, such as histamine and carbonyls, that cause rancidity.
Colorimeter and spectrophotometer, Mass Spectrometerprachann
It contains a brief knowledge on Introduction, Principle, Laws, Flow representation, Instrumentation, Applications
and Mass spectrometer
- Principle
- Instrumentation
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
1. DEMONSTRATION OF DIFFERENT INSTRUMENTS
USED IN BIOCHEMISTRY LABORATORY
Amit Jha
Lecturer
UCMS, Bhairahawa
2. Pipettes
Blow out Non blow out Volumetric
Measuring pipette Transfer pipette
Ostwald’s
Micropipette
1. Fixed
2. Variable
Pipettes are suction devices that are used to either
suck liquids into or expel liquids out of pipettes
3. Serological (Blow out) Pipette
• Calibrated all the way down to tip.
• Last drop of liquid needs to be blown out of the
tip to deliver full volume of pipette
4.
5.
6. Mohr (Non blow out) pipette
• Calibration marks don’t extend upto tip
but at a point above the tip.
7. Volumetric pipette
• Transfers a specific
volume of a given liquid.
• Shaped like a rolling pin,
with two thinner ends & a
thicker bulge in the middle.
• Have single calibration
mark.
8. Ostwald Follin pipette
• Similar to volumetric pipette, but bulb closer to
delivery tip.
• Used for accurate measurement of viscous fluids
[blood, serum]
9. Water bath [Constant temp. bath]
• Used to provide specific temp. to carry out different
chemical rxns.
Serum enzymes estimation 37◦C
Enzymatic methods 37◦C
Serological tests 56◦C
Saponification 60◦C-70◦C
• Components
• Nickel plated tank
• Strip heater: provide heat [below tank]
• Control knob: regulate temp.
10. Incubator
• Uses:
– Provide constant temp. during:
• Enzyme estimation
• Glucose, urea, creatinine, etc estimation
• Components:
– Doubled walled cabinet of mild steel
– Heating element
– Ventilation system [passes expanded inner air]
– Control knob [regulate temp.]
11.
12. Electronic balance
• Principle:
– When a mass is kept on pan, an electromagnetic
force is generated by magnet & coil.
– This electromagnetic force is converted into an
electric signal & displayed on a digital display.
13.
14. Centrifuge
• Centrifugation: process of using centrifugal force to
separate lighter particle suspended in a solution.
• Principle:
– When a particle suspended in a liquid is subjected to
centrifugal force (rotating the medium at high speed),
particles gets sedimented at bottom of tube.
– F = ω2.r
F: Centrifugal force
ω: Angular velocity
r: Radius
15. • Relative centrifugal force
– Centrifugal force expressed in terms of ‘g’
RCF = (1.19 x 10-5) . (rpm)2 . r
– RCF in terms of no. of ‘g’ is determined using a
nomogram.
– Eg: r = 7cm & rpm = 20,000
• RCF = 32000 x g
16. • Application of centrifuge
1. Separation of plasma or serum from blood.
2. Concentrate particles in urine sample, helpful for
microscopical examination
3. Ultracentrifugation
• To separate lipoproteins in serum sample
• To separate cell organelles
17. Colorimeter
• Principle:
– Beer's law: states that concentration of a subst. is
directly proportional to amount of light absorbed
• A ∞ C
– Lambert’s law: states that absorbnce is directly
proportional to the thickness of Pathlight.
• A ∞ T
– Beer-Lambert Law
• A ∞ C T
• A = E . C . T
A: Absorbance
C: Concentration
T: Thickness of Pathlight
E: Molar extinction coefficient
19. • Standard solution:
– As: Absorbance
– Cs: concentration
As = E . Cs . T ----------- [i]
• Unknown test solution
– At: Absorbance of test solution
– Ct: Concentration of a subst. in test solution [unknown]
At = E . Ct . T ------------- [ii]
As = E . Cs . T
At = E . Ct . T
• Ct = [At/As] . Cs
• Concentration of analyte in test solution is given by: ?????
20. • Application of Colorimeter
– Used for routine biochemical analysis of different
analytes in serum sample
• [Glucose, Total Protein, Albumin, Uric Acid, Total
Calcium, Phosphorus, Total Cholesterol, Triglyceride,
Urea, Creatinine, Bilirubin, etc]
21. Flame emission photometer
• Fast, simple & sensitive analytical method used to
determine inorganic metal ions [Na, K, Li, Ca etc] in
solution.
• Using hot flame metal is energized to higher energy state,
as isn’t stable, when gets back to normal low energy state
produces specific radiation.
22. Flame emission photometer
• Under const. & controlled condition, light intensity of
characteristics wavelength produced is directly
proportional to no. of atoms (concentration of metal of
interest in solution).
Na Yellow
K Violet
Li Red
Mg Blue
23. Flame emission photometer
• Instrumentation
Nebulizer For spraying of solution
Flame Generates heat for excitation of metal ions
Monochromator select light of single wavelength
Photo detector Quantifies emitted light by converting into electrical
impulse.
• Application
– Estimation of Na, K, Ca, Li, in various body fluids.
– Used in agricultural science to know about fertilizer
requirement of soil.
24. pH meter
• Principle:
– Based on measurement of potential difference
generated between reference (known potential) &
unknown electrode (glass electrode) (sensitive to H+).
– AgCl electrode: most commonly used.
– Thin glass membrane separates two electrodes.
– When electrode dipped in a solution, due to H+
movement, an electric potential is developed.
– Electric potential developed is amplified & converted into
direct pH reading on display unit.
25. • Electrophoresis
– Method used for separation of charged particle
– Principle
• When charged particle is run in an electric field,
charged particle moves towards oppositely charged
electrode.
• Rate of Migration ∞ e/m ratio
» e-: no. of charge
» m: mass of charged particle
– Due to differ in e/m ratio, rate of migration is
different for different charged particle.
26. • Procedure of separation of serum proteins:
– Sample [serum] is apllied to a strip of filter paper or
cellulose acetate.
– Both edges of strip is dipped in alkaline buffer solution.
– Being amphoteric, protein carry –ve charge in alkaline
medium.
– When current passed, proteins will move towards anode.
– Rate of migration of diff. proteins depends on:
• No. of charge on protein
• Mol. wt. of protein molecule.
27. • Procedure of separation of serum proteins:
– Serum proteins are separated into different bands:
» Albumins
» α1 globulins
» α2 globulins
» β globulins
» γ globulins
• Density of each band ∞ its concentration in serum
32. • Application of electrophoresis
– Used to separate charged particles like proteins, nucleic
acids, lipo-proteins.
– Used for diagnosis of:
» Multiple myeloma
» Nephrotic syndrome
» Cirrhosis of liver etc.
33. Chromatography
• Tech. used for separation of mixture of group of similar
substances by their continuous distribution & redistribution
into two phases. [stationary & mobile phase]
• Mobile phase: mixture of substance dissolved in liquid or
gas.
• Stationary Phase: porous solid matrix through which
mobile phase pass. [Solid / liquid / liquid coated on Solid
Surface]
34. Chromatography
• When mobile phase pass through stationary phase,
solutes gets distributed between two phase.
• High affinity to mobile phase: move faster & separated
from solutes having high affinity to stationary phase
• Distribution depends on physical property [Mass, Shape,
Size, Charge, Solubility, Adsorption property]
35. • Partition coefficient
conc. Of substance in Mobile phase
conc. Of substance in Stationary phase
Kd =
• Kd = 1: subst. gets distributed equally between two
phase
• Kd > 1: subst. have more affinity to mobile phase
• Kd <1 : subst. have more affinity to stationary phase
36. • Relative front
Dist. Covered by solvent from origin
Dist. Covered by solute from origin
Rf =
37. • Applications
– Used for identification & quantitative estimation of:
• Amino acids
• Proteins
• FA etc.