1) The study examines the anti-HIV activity and cytotoxicity of extracts and isolated compounds from Wrightia tomentosa leaf against HIV-1 and HIV-2. 2) All extracts and compounds showed cytotoxic effects against MT-4 cells with CC50 values ranging from 8-117 μg/ml. Indirubin displayed the highest cytotoxicity with a CC50 of 8.32 μg/ml. 3) The extracts and compounds did not show strong anti-HIV activity, with IC50 values all being greater than 50 μg/ml. However, indirubin provided some protection against viral infection at lower concentrations.