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CEFAPARQUES Project
Identification and characterization of preferential
habitats to cephalopods of commercial interest
New insights for a more suitable resource management
Participants
IIM (CSIC, Vigo) & IMEDEA (CSIC, Palma de Mallorca)
Ángel Guerra. Research coordinator
Ángel F. González Beatriz Morales - Nín
Manuel E. Garci Miguel Cabanellas - Reboredo
Jorge Hernández - Urcera Matias Calvo-Manazza
Alex Chamorro Miquel Palmer
CEFAPARQUES
Parque Nacional de las
Islas Atlánticas de Galicia
(PNIAG)
Parque Nacional de
Cabrera (PNC)
Project duration: April 2012- November 2014
Results: April 2012 – April 2014
PNIAG
 Octopus vulgaris spawning
habitat
 O. vulgaris juvenile /
subadult habitat
 O. vulgaris den ecology
 Cannibalism in the wild
PNIAG: Spawning habitat and zone
PNIAG: spawning habitat and area
April 2012 – April 2014
• 73 grids of 33.2 Ha each
• 24 grids sampled
• 5-25 m depth
• 4 visual censuses (surveys)
per month
• A total of 97 surveys
EST= Time of effective sampling
Dt= total immersion time
A= Time to get surface
Rt= recording time
EST = Dt - (A + Rt)
NSD = EST x ADS
PNIAG: visual censuses
NSD = net distance of sampling
ADS = diving estimated velocity
SAC = NSD x [HV x (Nd + 1 )]
SAC= swept area
HV= horizontal visibility
Nd= nº divers
A new sampling method to
estimate the swept area
PNIAG: spawning habitat and zone
Two-three divers
Images recorded (14 hours).
Substrate composition
GPS
CTD: Oceanographic parameters
GAMs: habitat characterization (season, depth, temperature, chlorophyll, substrate, predators,
fishing impact … )  presence/absence of egg masses and spawning nests
PNIAG: spawning habitat and zone
The highest probability of occurrence of spawns and dens for females O.
vulgaris nesting (spawning nests) were obtained in the north zone of the
Cíes islands and during spring-summer season
Egg clusters: 24
Female spawning nests : 54
Cíes Islands
Preferential spawning
area and season
North
Spring-Summer
PNIAG: spawning area
Recommendation: Total Protection
inside the MPA (58 ha 5.8 % of total
marine Cíes realm) in that season
First step for O. vulgaris ecosystem
approach to fishery management
PNIAG: spawning habitat and zone
Females behavior during reproduction: recorded and described
Aquí va el video de la hembra
cuidando la puesta, no puedo enviarlo
por email porque ocupa demasiado
espacio
PNIAG: juveline-subadult habitat
Rodas inlet = Sand seabed
30 ha, 5-21 m depth
4 divers
3 visual surveys in June 2013
(O. vulgaris fishery season
closed)
1 survey in October 2013
(O. vulgaris fishery season open)
Recorded data:
• Depth
• Bottom temperature
• Presence/absence in den
• Octopus size
• Bivalves in the midden
• Den diameter
PNIAG: juvenile-subadult habitat
PNIAG: juvenile-subadult habitat
No statistical differences in occupation rate between both (closed or open)
fishing seasons (VC1-VC2-VC3 and VC4)
VC: visual census number
SA: swept area (m2)
D: average depth (m)
APD: area per den (m2)
OD: occupied dens
ARDO: average rate of
occupancy (%)
D/1000: mean number
VC DR DF ARDO DDA ± SD
1,2,3
5.0-10.0 27 92,6 19,4 ± 8,61
10.0-15.0 6 50 14,83 ± 6,24
5.0-10.0 9 100 17,44 ± 5,08
4 10.0-15.0 6 50 16,83 ± 5,81
15.0-21.0 5 40 17,6 ± 3,51
VC S A D APD OD ARDO D/1000
1 3330 11 302,73 10 90,91 3,30
2 2910 14 207,86 11 78,57 4,81
3 2350 8 293,75 7 87,50 3,40
4 5145 20 257,25 14 70 3,89
DR: depth range (m)
DF: number of dens found
ARDO: average rate of occupancy
(%)
DDA: den diameter average (cm)
SD: standard deviation
Visuals surveys in Rodas inlet
Dens data in Rodas inlet
PNIAG: juvenile-subadult habitat
5 10 15 20
−4−202
Depth
PartialforDepth
5 10 15 20
0.00.20.40.60.81.0
Depth
response
Prediction of a den occupied
by an octopus: Decreased with z
Partial effect of depth in
presence/absence of octopus in
a den: Decreased with z
16 Kg octopus in the Park
PNIAG: juvenile-subadult habitat
• Preferred habitat: SBT 15 – 16 ºC and
SBS ≈ 36.0
• Depth 5-21 m, maybe related with:
⁻ prey presence, specially
bivalves
⁻ Soft bottoms with bioclast
• Presence of double and triple dens in
Rodas  asocial?  Further studies
needed
This area should
be also protected
1170 juveniles O. vulgaris
in Rodas inlet between 5-
21 m depth. (1.12% of the
Cíes marine area)
• Den typology:
A. Dug den in soft
sediment and
reinforced with
shells
B. Dug den under a
rock
C. Empty shell or
carapace
D. Human wastes
PNIAG: Dwellers in dens on sandy
bottoms: Ecological and behavioral traits
of Octopus vulgaris
PNIAG: den ecology
Shell collection: bivalves in the
middens: old and none of then drilled.
Main food : razors (Ensis arcuactus)
PNIAG: den ecology
- Deep holes reinforced with Pecten
maximus
- Shells collected at the mouth of the hole
were old with algae, polichaetes and
bryozoan incrustation
- Large and well build holes were occupied
by small octopuses
- The middens were 3 times larger the hole
size
Permanent dens
PNIAG: Cannibalistic behavior
Cannibalism has been
observed and registered
three times within PNIAG
• Inter-cohort cannibalism
• Both males and females are cannibal
• Arms were the first part of the body
eaten
• Other preys were abundant in the
surrenders
• Predator was 20-25 % larger than prey
PNIAG: General conclusions for
O. vulgaris
• The inner submarine area of the Cíes
Islands is an Essential Fish Habitat for
O. vulgaris.
• Two habitat and zones: one of
breeding and spawning and other of
growth will be proposed to the
National Park authorities to be
protected.
• Useful tool for resources management
of commercial interest (ecosystem
approach).
• Informative and educational
documentary: Goods and services of a
marine protected area.
http://www.cefaparques.tk/
PNIAG: Sepia officinalis
MES T D Tf
E F M A M J J A S O N D
P 4 9 5 7 1 5 31 10-
15
11-
14
E 4(2) 3(2) 4(2) 6(2) 8(2) 1(1) 12(2) 2(2) 3(2) 21(2) 4(2) 2(2)
1(3)
71
P= egg-laying
E= individuals registered and size (1:<90 mm LM, 2: 91-180, 3: >180)
D= deep (m)
Tf = Bottom temperature (ºC)
PNIAG: Sepia officinalis
Surveys of S. officialis in the inner side of the Cies Islands .
Total of 71, 58 % close Viños islet
Egg-laying Winter-spring time (in gorgonians and spirograph
PNIAG: Loligo vulgaris
Registered once
PNC National Park of Cabrera
Results only for Loligo
vulgaris
No egg laying Sepia or
Octopus
Predator pressure
(Epinephelus marginatus)
PNC Spawning areas
30 DACs Dispositives for egg laying
Recovered one a month (june 2012 june
2013)
141 egg laying associated collected and
counted
PNC Spawning areas
Zero-Inflated Poisson (ZIP)
(Number of egg laying per DAC per
month)
Variables:
• Habitat
• Depth
• SST
• SSC
• Presence/absence of Lepas
anatifera,
currents…
Spatial distribution of egg
masses Loligo vulgaris for
DAC
Spawn all year, spring peak
(may)
242 egg masses, 72,3 % in DAC
in sand floors
15-50 m deep ( avoid surficial
waters, above all sin summer)
PNC Spawning areas
All year spawning:
• surficial waters in winter
• deep waters in summer
spawning migrations
PNC Spawning areas
PNC Spawning areas
DAC: useful technique to characterize L. vulgaris habitat
Spawning area depends on ocean bottom, deep, SST
Spawning peak maybe related with mesozooplankton availability
Migration for spawning
Management improvement: temporal - spatial closure of
the fish area

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CEFAPARQUES PROJECT: ICES CEPHALOPODS WORKING GROUP

  • 1. CEFAPARQUES Project Identification and characterization of preferential habitats to cephalopods of commercial interest New insights for a more suitable resource management
  • 2. Participants IIM (CSIC, Vigo) & IMEDEA (CSIC, Palma de Mallorca) Ángel Guerra. Research coordinator Ángel F. González Beatriz Morales - Nín Manuel E. Garci Miguel Cabanellas - Reboredo Jorge Hernández - Urcera Matias Calvo-Manazza Alex Chamorro Miquel Palmer
  • 3. CEFAPARQUES Parque Nacional de las Islas Atlánticas de Galicia (PNIAG) Parque Nacional de Cabrera (PNC) Project duration: April 2012- November 2014 Results: April 2012 – April 2014
  • 4. PNIAG  Octopus vulgaris spawning habitat  O. vulgaris juvenile / subadult habitat  O. vulgaris den ecology  Cannibalism in the wild
  • 6. PNIAG: spawning habitat and area April 2012 – April 2014 • 73 grids of 33.2 Ha each • 24 grids sampled • 5-25 m depth • 4 visual censuses (surveys) per month • A total of 97 surveys
  • 7. EST= Time of effective sampling Dt= total immersion time A= Time to get surface Rt= recording time EST = Dt - (A + Rt) NSD = EST x ADS PNIAG: visual censuses NSD = net distance of sampling ADS = diving estimated velocity SAC = NSD x [HV x (Nd + 1 )] SAC= swept area HV= horizontal visibility Nd= nº divers A new sampling method to estimate the swept area
  • 8. PNIAG: spawning habitat and zone Two-three divers Images recorded (14 hours). Substrate composition GPS CTD: Oceanographic parameters GAMs: habitat characterization (season, depth, temperature, chlorophyll, substrate, predators, fishing impact … )  presence/absence of egg masses and spawning nests
  • 9. PNIAG: spawning habitat and zone The highest probability of occurrence of spawns and dens for females O. vulgaris nesting (spawning nests) were obtained in the north zone of the Cíes islands and during spring-summer season Egg clusters: 24 Female spawning nests : 54 Cíes Islands
  • 10. Preferential spawning area and season North Spring-Summer PNIAG: spawning area Recommendation: Total Protection inside the MPA (58 ha 5.8 % of total marine Cíes realm) in that season First step for O. vulgaris ecosystem approach to fishery management
  • 11. PNIAG: spawning habitat and zone Females behavior during reproduction: recorded and described
  • 12. Aquí va el video de la hembra cuidando la puesta, no puedo enviarlo por email porque ocupa demasiado espacio
  • 13. PNIAG: juveline-subadult habitat Rodas inlet = Sand seabed 30 ha, 5-21 m depth 4 divers 3 visual surveys in June 2013 (O. vulgaris fishery season closed) 1 survey in October 2013 (O. vulgaris fishery season open)
  • 14. Recorded data: • Depth • Bottom temperature • Presence/absence in den • Octopus size • Bivalves in the midden • Den diameter PNIAG: juvenile-subadult habitat
  • 15. PNIAG: juvenile-subadult habitat No statistical differences in occupation rate between both (closed or open) fishing seasons (VC1-VC2-VC3 and VC4) VC: visual census number SA: swept area (m2) D: average depth (m) APD: area per den (m2) OD: occupied dens ARDO: average rate of occupancy (%) D/1000: mean number VC DR DF ARDO DDA ± SD 1,2,3 5.0-10.0 27 92,6 19,4 ± 8,61 10.0-15.0 6 50 14,83 ± 6,24 5.0-10.0 9 100 17,44 ± 5,08 4 10.0-15.0 6 50 16,83 ± 5,81 15.0-21.0 5 40 17,6 ± 3,51 VC S A D APD OD ARDO D/1000 1 3330 11 302,73 10 90,91 3,30 2 2910 14 207,86 11 78,57 4,81 3 2350 8 293,75 7 87,50 3,40 4 5145 20 257,25 14 70 3,89 DR: depth range (m) DF: number of dens found ARDO: average rate of occupancy (%) DDA: den diameter average (cm) SD: standard deviation Visuals surveys in Rodas inlet Dens data in Rodas inlet
  • 16. PNIAG: juvenile-subadult habitat 5 10 15 20 −4−202 Depth PartialforDepth 5 10 15 20 0.00.20.40.60.81.0 Depth response Prediction of a den occupied by an octopus: Decreased with z Partial effect of depth in presence/absence of octopus in a den: Decreased with z 16 Kg octopus in the Park
  • 17. PNIAG: juvenile-subadult habitat • Preferred habitat: SBT 15 – 16 ºC and SBS ≈ 36.0 • Depth 5-21 m, maybe related with: ⁻ prey presence, specially bivalves ⁻ Soft bottoms with bioclast • Presence of double and triple dens in Rodas  asocial?  Further studies needed This area should be also protected 1170 juveniles O. vulgaris in Rodas inlet between 5- 21 m depth. (1.12% of the Cíes marine area)
  • 18. • Den typology: A. Dug den in soft sediment and reinforced with shells B. Dug den under a rock C. Empty shell or carapace D. Human wastes PNIAG: Dwellers in dens on sandy bottoms: Ecological and behavioral traits of Octopus vulgaris
  • 19. PNIAG: den ecology Shell collection: bivalves in the middens: old and none of then drilled. Main food : razors (Ensis arcuactus)
  • 20. PNIAG: den ecology - Deep holes reinforced with Pecten maximus - Shells collected at the mouth of the hole were old with algae, polichaetes and bryozoan incrustation - Large and well build holes were occupied by small octopuses - The middens were 3 times larger the hole size Permanent dens
  • 21. PNIAG: Cannibalistic behavior Cannibalism has been observed and registered three times within PNIAG • Inter-cohort cannibalism • Both males and females are cannibal • Arms were the first part of the body eaten • Other preys were abundant in the surrenders • Predator was 20-25 % larger than prey
  • 22. PNIAG: General conclusions for O. vulgaris • The inner submarine area of the Cíes Islands is an Essential Fish Habitat for O. vulgaris. • Two habitat and zones: one of breeding and spawning and other of growth will be proposed to the National Park authorities to be protected. • Useful tool for resources management of commercial interest (ecosystem approach). • Informative and educational documentary: Goods and services of a marine protected area.
  • 24.
  • 25. PNIAG: Sepia officinalis MES T D Tf E F M A M J J A S O N D P 4 9 5 7 1 5 31 10- 15 11- 14 E 4(2) 3(2) 4(2) 6(2) 8(2) 1(1) 12(2) 2(2) 3(2) 21(2) 4(2) 2(2) 1(3) 71 P= egg-laying E= individuals registered and size (1:<90 mm LM, 2: 91-180, 3: >180) D= deep (m) Tf = Bottom temperature (ºC)
  • 26. PNIAG: Sepia officinalis Surveys of S. officialis in the inner side of the Cies Islands . Total of 71, 58 % close Viños islet Egg-laying Winter-spring time (in gorgonians and spirograph
  • 28. PNC National Park of Cabrera Results only for Loligo vulgaris No egg laying Sepia or Octopus Predator pressure (Epinephelus marginatus)
  • 29. PNC Spawning areas 30 DACs Dispositives for egg laying Recovered one a month (june 2012 june 2013) 141 egg laying associated collected and counted
  • 30. PNC Spawning areas Zero-Inflated Poisson (ZIP) (Number of egg laying per DAC per month) Variables: • Habitat • Depth • SST • SSC • Presence/absence of Lepas anatifera, currents…
  • 31. Spatial distribution of egg masses Loligo vulgaris for DAC Spawn all year, spring peak (may) 242 egg masses, 72,3 % in DAC in sand floors 15-50 m deep ( avoid surficial waters, above all sin summer) PNC Spawning areas
  • 32. All year spawning: • surficial waters in winter • deep waters in summer spawning migrations PNC Spawning areas
  • 33. PNC Spawning areas DAC: useful technique to characterize L. vulgaris habitat Spawning area depends on ocean bottom, deep, SST Spawning peak maybe related with mesozooplankton availability Migration for spawning Management improvement: temporal - spatial closure of the fish area