This document provides information about horizontal gene transfer through various mechanisms like transformation, transduction, and conjugation. It discusses that horizontal gene transfer is the transmission of genes between organisms independently of vertical descent or reproduction. The key mechanisms of horizontal gene transfer in bacteria are transformation (uptake of naked DNA from environment), transduction (transfer via bacteriophages), and conjugation (direct cell-to-cell contact via plasmids or pili). Horizontal gene transfer plays an important role in the evolution and adaptation of bacteria by allowing them to acquire new traits from other species.
Autonomously replicating circular fragment present in DNA is called plasmids.
The term plasmid was first introduced by American molecular biologist Joshua Lederberg in1952.
An episome is a plasmid capable of inserting DNA into the host chromosome.
Because of their ability to transfer DNA from one bacterium to another, plasmids are extensively used in recombinant DNA technology or genetic engineering.
Autonomously replicating circular fragment present in DNA is called plasmids.
The term plasmid was first introduced by American molecular biologist Joshua Lederberg in1952.
An episome is a plasmid capable of inserting DNA into the host chromosome.
Because of their ability to transfer DNA from one bacterium to another, plasmids are extensively used in recombinant DNA technology or genetic engineering.
It is the basics of vector cloning which necessary for every and each student who is intrested in biotechnology. It is only starting, if you want to more than this then please comment on it.
BACTERIAL RECOMBINATION,PLASMIDS AND EPISOMESsushma93
Genetic recombination - transfer of DNA from one organism (donor) to another organism (recipient). The transferred donor DNA may then be integrated into the recipient's genetic material by various mechanisms
Bacterial recombination occurs in three ways
Transformation
Transduction
Conjugation
transformation in bacteria is a classical example of horizontal gene transfer which leads to enhanced survivability and also introduction of variations that may lead to evolution
the horizontal gene transfer in bacteria is not only important for survival but has its evolutionary significance too. this presentation is a prelude to the three classical types of HGT in bacteria
Bacterial cells rise their level of genetic diversity and overcome their lack of sexuality by horizontal DNA transfer.
Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells.
The main difference between plasmid and vectors is that plasmid is an extra-chromosomal element of mainly bacterial cells whereas vector is a vehicle that carries foreign DNA molecules into another cell. Vectors are mainly used in the recombinant DNA technology to introduce foreign DNA molecules into cells.
It is the basics of vector cloning which necessary for every and each student who is intrested in biotechnology. It is only starting, if you want to more than this then please comment on it.
BACTERIAL RECOMBINATION,PLASMIDS AND EPISOMESsushma93
Genetic recombination - transfer of DNA from one organism (donor) to another organism (recipient). The transferred donor DNA may then be integrated into the recipient's genetic material by various mechanisms
Bacterial recombination occurs in three ways
Transformation
Transduction
Conjugation
transformation in bacteria is a classical example of horizontal gene transfer which leads to enhanced survivability and also introduction of variations that may lead to evolution
the horizontal gene transfer in bacteria is not only important for survival but has its evolutionary significance too. this presentation is a prelude to the three classical types of HGT in bacteria
Bacterial cells rise their level of genetic diversity and overcome their lack of sexuality by horizontal DNA transfer.
Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells.
The main difference between plasmid and vectors is that plasmid is an extra-chromosomal element of mainly bacterial cells whereas vector is a vehicle that carries foreign DNA molecules into another cell. Vectors are mainly used in the recombinant DNA technology to introduce foreign DNA molecules into cells.
1a- What are three ways that bacteria can exchange genetic informati.pdfeyelineoptics
1a- What are three ways that bacteria can exchange genetic information? How are these similar
and how are they different? How can they be used to map bacterial genomes?
b) Explain how genes in phages are mapped. (This is different from the above question)
Solution
Ques-1: What are three ways that bacteria can exchange genetic information? How are these
similar and how are they different? How can they be used to map bacterial genomes?
Answer:
Horizontal gene transfer (HGT) or lateral gene transfer is the transfer of genes between two
organisms that are phylogenetically unrelated but can readily exchange genes by conjugation or
transduction or transformation. HGT commonly occurs in prokaryotes and it plays a key role in
their evolution. HGT accelerates evolution.
Horizontal gene transfer: 1. conjugation, 2. transformation; 3. transduction
Similarities & differences:
Horizontal gene transfer is the transfer of genomes by between bacteria by the routes that does
not involve parent-offspring transmission. Conjugation, transduction and transformation are the
three mechanism of horizontal gene transfer.
In the above case, it has clearly illustrated that the conditions, which can successfully prevents
transduction and abolish transfer of chromosomal DNA from one bacterial cell to another by
generalized transduction with P22 bacteriophage. Initially recipient cell- surface receptors are
important for the attachment of the bacteriophage (P22) and finally spikes of phage enable
bacterial membrane to lyse finally incorporate phage genome into the bacterial cell for
integration. Therefore, for efficient transduction, presence of recipient cell- surface receptors is
essential in the culture otherwise, bacteria exert resistant effects to the phages.
Transformation type of gene transfer occurs between bacteria. The genes released by dead
bacteria can be transferred to the live bacteria, and get integrated into its genome, which results
in genetic recombination of the host.
Transduction is also involving the genetic material transfer, but this needs bacteriophage as
media. The bacteriophage infects the bacteria in lytic cycle and releases the new virions, the
capsids of some of these virions carry the bacterial DNA fragments into the new host bacteria,
followed by genetic recombination of the host bacteria.
Conjugation: It is the transfer of genetic material from cell to cell by means of direct contact or a
bridge like structure. Bacterial conjugation needs the conjugation tube for genome transfer, both
transduction and transformation does not need this.
Mapping the genome -mechanisms
F factor is mainly pertaining to a class of conjugate plasmids, which meticulously originated to
control sexual functions in prokaryotes via fertility inhibition. The origin of F-factor is mainly
with most common functional segments such as gene traJ, Ori, OriC. The structure of this F
factor contains OriT for origin of transfer and acts as a starting point for gene transmission.
Prokaryotes can exchange DNA with eukaryotes, although the mechanisms behind this process are not well understood. Suspected mechanisms include conjugation and endocytosis, such as when a eukaryotic cell engulfs a prokaryotic cell and gathers it into a special membrane-bound vesicle for degradation.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
DERIVATION OF MODIFIED BERNOULLI EQUATION WITH VISCOUS EFFECTS AND TERMINAL V...Wasswaderrick3
In this book, we use conservation of energy techniques on a fluid element to derive the Modified Bernoulli equation of flow with viscous or friction effects. We derive the general equation of flow/ velocity and then from this we derive the Pouiselle flow equation, the transition flow equation and the turbulent flow equation. In the situations where there are no viscous effects , the equation reduces to the Bernoulli equation. From experimental results, we are able to include other terms in the Bernoulli equation. We also look at cases where pressure gradients exist. We use the Modified Bernoulli equation to derive equations of flow rate for pipes of different cross sectional areas connected together. We also extend our techniques of energy conservation to a sphere falling in a viscous medium under the effect of gravity. We demonstrate Stokes equation of terminal velocity and turbulent flow equation. We look at a way of calculating the time taken for a body to fall in a viscous medium. We also look at the general equation of terminal velocity.
Salas, V. (2024) "John of St. Thomas (Poinsot) on the Science of Sacred Theol...Studia Poinsotiana
I Introduction
II Subalternation and Theology
III Theology and Dogmatic Declarations
IV The Mixed Principles of Theology
V Virtual Revelation: The Unity of Theology
VI Theology as a Natural Science
VII Theology’s Certitude
VIII Conclusion
Notes
Bibliography
All the contents are fully attributable to the author, Doctor Victor Salas. Should you wish to get this text republished, get in touch with the author or the editorial committee of the Studia Poinsotiana. Insofar as possible, we will be happy to broker your contact.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
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The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
2. A gene determines a particular trait by encoding
for a specific polypeptide in a given organism.
Because the genetic code is (almost) universal,
an organism can potentially express a new trait
if the appropriate gene is introduced into its
genome. A gene determines the phenotype of
organism. A gene is the functional unit of
heredity. Each chromosome carry a linear array
of multiple genes. Each gene represents segment
of DNA responsible for synthesis of RNA or
protein product. A gene is considered to be unit
of genetic information that controls specific
aspect of phenotype.
GENE
3. GENE TRANSFER (GT):-
The introduction of new DNA into an existing organism’s cell, usually by vectors such as
plasmids and modified viruses. The transfer of genes between species is called GENE
TRANSFER. The new organism created is called a transgenic. The insertion of unrelated
therapeutic genetic information in the form of DNA into target cells.
The directed desirable gene transfer from one Organism to another and the subsequent
stable integration and expression of foreign gene into the genome is referred to as genetic
transformation. Transient transformation occur when DNA is not integrated into host
genome.
Two Types of gene transfer:-
1. HORIZONTAL GT- Horizontal gene transfer can be described as the transfer of genetic
information between two independent organisms.
2. VERTICAL GT- Vertical gene transfer is the transfer of genetic information from parent
to progeny. Germline, Live birth, Patrilineal, Aposymbiotic.
4. DNA transfer by natural methods:-
1. Conjugation
2. Bacterial transformation
3. Retroviral transduction
4. Agrobacterium mediated transfer
DNA TRANSFER BY ARTIFICIAL
METHODS:-
Physical methods-
1. Microinjection
2. Biolistics transformation
Chemical methods-
1. DNA transfer by calcium phosphate
method
2. Liposome mediated transfer
Electrical methods-
1. Electroporation
5. Horizontal gene transfer (Lateral gene transfer):-
The transmission of DNA (deoxyribonucleic acid) between different genomes is referred to
as horizontal gene transfer, sometimes known as lateral gene transfer. Between distinct
species, such as prokaryotes and eukaryotes , and between the three DNA-containing
organelles of eukaryotes—the nucleus, the mitochondrion, and the chloroplast—
horizontal gene transfer is known to happen. Vertical gene transfer, or the passing of genetic
material from parents to children during reproduction, is distinct from the acquisition of
DNA by horizontal gene transfer. Gene transfer in bacteria was studied by Griffith in 1928,
there are the following types of gene transfer mechanism.
Mechanism-
1. Transformation
2. Transduction
3. Conjugation
4. Transposon transfer
5. Fusion of cells
6. Gene transfer in prokaryotes-
Transformation
Transduction
Conjugation
Archaeal DNA transfer
Gene transfer in eukaryotes-
Organelle to nuclear genome
Organelle to organelle
Virus to plants
Bacteria to fungi
Bacteria to plants
Bacteria to animals
Endosymbiont to insects and nematodes
Plant to plant
Plant to animals
Plant to fungus
Fungi to insects
Fungi to fungi
Animal to animal
Animal to bacteria
Human to protozoa
7. TRANSFORMATION:-
The genetic alteration of a cell resulting from the introduction, uptake and expression of
foreign genetic material (DNA) in molecular biology. This can be done to Bacteria, Fungi,
Plants, and Animal cells.
Transformation principle was demonstrated
in 1944 by Oswald Avery, Colin MacLeod,
and Maclyn McCarty, who showed gene
transfer in Streptococcus pneumoniae was
pure DNA. Avery, Macleod and McCarty
call the uptake and incorporation of DNA by
bacteria transformation. A few bacteria, such
as Neisseria gonorrhoeae, Neisseria
meningitidis, Hemophilus influenzae,
Legionella pneomophila, Streptococcus
pneumoniae, and Helicobacter pylori tend to
be naturally competent and transformable.
8. Bacteria – transformation refers to a genetic
change brought about by picking up naked
strands of DNA and expressing it. –
Competence refers to the state of being able
to take up DNA. – Two different forms of
competence should be distinguished, natural
and artificial. The process of gene transfer
by transformation doesn’t require a living
donor cell but only requires the presence of
persistent DNA in the environment.
The factors that regulate natural competence
vary between various genera. Transformed
bacteria used as host cell in cloning
procedures, in DNA linkage studies,
generation of Cdna libraries, express large
amounts of proteins and enzyme.
9. CONJUGATION:-
Bacterial conjugation was discovered by Lederberg And Tatum in1946 in Esch.coliK12
strains. During conjugation , DNA is transferred from one bacterium to another. After the
donor cell pulls itself close to the recipient using a structure called a pilus, DNA is
transferred between cells. In most cases, this DNA is in the form of a plasmid. Donor cells
typically act as donors because they have a chunk of DNA called the fertility factor (or F
factor). This chunk of DNA codes for the proteins that make up the sex pilus. It also
contains a special site where DNA transfer during conjugation begins. Conjugation is
encoded by plasmids or transposons.
Conjugation are of different types:-
1. F+ conjugation
2. Hfr conjugation
3. Resistant plasmid conjugation
4. Sexduction
10. Conjugation steps-
Step 1:The pilus enables direct contact
between the donor and the recipient cells.
Step 2: Because the F-plasmid consists
of a double stranded DNA molecule
forming a circular structure, i.e., it is
attached on both ends, an enzyme
(relaxase, or relaxosome when it forms a
complex with other proteins) nicks one
of the two DNA strands of the F plasmid
and this strand (also called T-strand) is
transferred to the recipient cell.
11. Step 3: Donor cell and the
recipient cell, both containing
single-stranded DNA, replicate it
and thus end up forming a double-
stranded F-plasmid identical to the
original F-plasmid. (see below),
the old recipient cell is now a
donor cell with the F-plasmid and
the ability to form pili, just as the
original donor cell was. Now both
cells are donors or F+.
12. TRANSDUCTION:-
Transduction involves the transfer of a DNA fragment from one bacterium to another by a
bacteriophage. Bacteriophages, also known as phages, are viruses that infect and replicate
only in bacterial cells. They are ubiquitous in the environment and are recognized as the
most abundant biological agent on earth. They are extremely diverse in size, morphology,
and genomic organization.
13. There are two forms of
transduction: generalized
transduction(LYTIC PHAGE)
and specialized transduction
(LYSOGENIC PHAGE)
If the lysogenic cycle is
adopted the phage chromosome
is integrated (by covalent
bonds) into the bacterial
chromosome where it can
remain dormant for thousands
of generation. The lytic cycle
leads to production of new
phage which is released by lysis
of host.
14. AGROBACTERIUM
MEDIATED TRANSFERS:-
Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses
horizontal gene transfer to cause tumors in plants
Agrobacterium is a phytopathogen that infects plants through wound sites, causing
crown gall disease, and is one of the most popular plant transformation tools used in
agriculture to date.
Agrobacterium tumefaciens harboring the tumor-inducing (Ti) plasmid induces Crown
galls on stems, roots and crowns of numerous dicot angiosperm species and some
gymnosperms. The neoplastic tumor-like cell growth is induced by expression of the
oncogenes residing in the transferred-DNA (T-DNA) transported from these bacteria
into the plant nucleus and integrated into the plant genome.
15. Essential component of T-dna-
1. The first essential component is the T-DNA, defined by conserved 25-base pair
imperfect repeats at the ends of the T-region called border sequences.
2. The second is the virulence (vir) region, which is composed of at least seven major
loci (virA, virB, virC, virD, virE, virF, and virG) encoding components of the bacterial
protein machinery mediating T-DNA processing and transfer.
Major steps of the Agrobacterium tumefaciens-mediated gene transfer process:-
(1) Attachment of A. tumefaciens to the plant cells.
(2) Sensing plant signals by A. tumefaciens and regulation of virulence genes in
bacteria following transduction of the sensed signals.
(3) Generation and transport of T-DNA and virulence proteins from the bacterial cells
into plant cells.
(4) Nuclear import of T-DNA and effector proteins in the plant cells.
(5) T-DNA integration and expression in the plant genome.