GenBank format and FASTA format as homo sapiens pepsin as an example bioinformatics practical 1st experiment ; sequence retrival from nucleotide sequence from NCBI

1. Homo sapiens cDNA FLJ60007 complete cds, highly
similar to Pepsin A precursor (EC 3.4.23.1)
GenBank: AK301293.1
LOCUS AK301293 980 bp mRNA linear PRI 24-JUL-
2008
DEFINITION Homo sapiens cDNA FLJ60007 complete cds, highly similar to Pepsin
A
precursor (EC 3.4.23.1).
ACCESSION AK301293
VERSION AK301293.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 980)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) Contact:Takao Isogai Reverse Proteomics
Research Institute; 1-9-11 Kaji-cho, Chiyoda-ku, Tokyo 101-0044,
Japan E-mail :flj-cdna@nifty.com
COMMENT Human cDNA sequencing project focused on splicing variants of
mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan
and

2. HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..980
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="STOMA2001970"
/tissue_type="stomach"
/clone_lib="STOMA2"
/note="cloning vector: pME18SFL3"
CDS 53..820
/note="unnamed protein product; highly similar to Pepsin
A
precursor (EC 3.4.23.1)"
/codon_start=1
/protein_id="BAG62851.1"
/translation="MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKK
HNLNPARKYFPQWKAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVLFDTGSSNLWV
PSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGCQAIVDTGTSLLTGPTSPIANI
QSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQGM
NLPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA"
ORIGIN
1 gcaccttcct cccgtcttgc cttctccctc gagttgggac ccgggaagaa ccatgaagtg
61 gctgctgctg ctgggtctgg tggcactctc tgagtgcatc atgtacaagg tccccctcat
121 cagaaagaag tccttgaggc gcaccctgtc cgagcgtggc ctgctgaagg acttcctgaa
181 gaagcacaac ctcaacccag ccagaaagta cttcccccag tggaaggctc ccaccctggt
241 agatgaacag cccctggaga actacctgga tatggagtac ttcggcacta tcggcatcgg
301 aactcctgcc caggatttca ctgtcctctt tgacaccggc tcctccaacc tgtgggtgcc
361 ctcagtctac tgctccagtc ttgcctgcac caaccacaac cgcttcaacc ctgaggattc
421 ttccacctac cagtccacca gcgagacagt ctccatcacc tacggctgcc aggccattgt
481 tgacaccggc acctctctgc tgaccggccc aaccagcccc attgccaaca tccagagcga
541 catcggagcc agcgagaact cagatggcga catggtggtc agctgctcag ccatcagcag
601 cctgcccgac atcgtcttca ccatcaatgg agtccagtac cccgtgccac ccagtgccta
661 catcctgcag agcgagggga gctgcatcag tggcttccag ggcatgaacc tccccaccga
721 atctggagag ctttggatcc tgggtgatgt cttcatccgc cagtacttta ccgtcttcga
781 cagggcaaac aaccaggtcg gcctggcccc cgtggcttaa gcctaagtct cttcagccac

3. 841 ctcccaggaa gatctggcct ctgtcctgtg cccactttag atgtatctaa ttctcctgac
901 tgttcttccc aggggagtgt ggaggtcttg gccctgttcc ctgtcctacc aataacgtag
961 aataaaaaca taacccactg
//
FASTA ;
>AK301293.1 Homo sapiens cDNA FLJ60007 complete cds, highly similar to Pepsin
A precursor (EC 3.4.23.1)
GCACCTTCCTCCCGTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG
CTGGGTCTGGTGGCACTCTCTGAGTGCATCATGTACAAGGTCCCCCTCATCAGAAAGAAGTCCTTGAGGC
GCACCCTGTCCGAGCGTGGCCTGCTGAAGGACTTCCTGAAGAAGCACAACCTCAACCCAGCCAGAAAGTA
CTTCCCCCAGTGGAAGGCTCCCACCCTGGTAGATGAACAGCCCCTGGAGAACTACCTGGATATGGAGTAC
TTCGGCACTATCGGCATCGGAACTCCTGCCCAGGATTTCACTGTCCTCTTTGACACCGGCTCCTCCAACC
TGTGGGTGCCCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAACCCTGAGGATTC
TTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGCTGCCAGGCCATTGTTGACACCGGC
ACCTCTCTGCTGACCGGCCCAACCAGCCCCATTGCCAACATCCAGAGCGACATCGGAGCCAGCGAGAACT
CAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCGACATCGTCTTCACCATCAATGG
AGTCCAGTACCCCGTGCCACCCAGTGCCTACATCCTGCAGAGCGAGGGGAGCTGCATCAGTGGCTTCCAG
GGCATGAACCTCCCCACCGAATCTGGAGAGCTTTGGATCCTGGGTGATGTCTTCATCCGCCAGTACTTTA
CCGTCTTCGACAGGGCAAACAACCAGGTCGGCCTGGCCCCCGTGGCTTAAGCCTAAGTCTCTTCAGCCAC
CTCCCAGGAAGATCTGGCCTCTGTCCTGTGCCCACTTTAGATGTATCTAATTCTCCTGACTGTTCTTCCC
AGGGGAGTGTGGAGGTCTTGGCCCTGTTCCCTGTCCTACCAATAACGTAGAATAAAAACATAACCCACTG