The ASR Zebrafish facility provides several services including fish housing, embryo production, gene manipulation, and phenotypic analysis. Gene expression can be manipulated through RNA, DNA, and morpholino microinjections. Phenotypic analysis includes visualization of morphology, histology, fluorescence, and apoptosis. The facility also supports toxicity and drug screening in zebrafish using 96 or 384 well formats with wildtype or transgenic lines. Shared resources are partially supported by Georgetown and Howard University.

1. Animal Shared Resource (ASR) Zebrafish Eric Glasgow, PhD [email_address] 687-7350

2. ASR Zebrafish Services Fish Housing and Care Embryo Production Genotyping Manipulation of Gene Expression - Morpholino, RNA, and DNA microinjections Toxicity and Drug Screening Phenotypic Analysis - Visualization, Documentation and Interpretation Consulting and Training

3. Manipulation of Gene Expression in Zebrafish RNA Morpholino (MO) (knockdown) cDNA (transient & mosaic) cDNA (stable transgenic)

4. Phenotype analyses in zebrafish Morphology and/or histology whole-mount in situ hybridization histochemistry (ISHH) whole-mount immunohistochemistry (IHC) fluorescent marker (EGFP) expression (living embryos) acridine orange apoptosis assay (living embryos) cell transplantation and lineage tracing (living embryos)

5. Toxicity and drug screening in zebrafish 96 or 384 well format wt Ezrin MO1 wt NCS305787 wt NSC668394 wt NSC668394 Novel compound activity assay Small compound library screening Transgene based assays

6. Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01 In your publications, remember to mention support provided from the GHUCCTS.