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Histological Structure of 
Pituitary gland 
Asst. Prof Chetana 
Kanekar 
M.Sc ( SET life sciences)
Introduction 
 The pituitory gland or hypophysis is structurally 
and functionally most complex endocrine gland. 
 Located in th ebrain attached by slender 
hypophysial or infundibular stalk,just behind the 
optic chiasma. 
 The pituitory gland has double origin as it is partly 
derived from oral ectoderm and partly from the 
brain. 
 The pituitory gland has two main parts: 
I. Adenohypophysis 
II. Neurohypophysis
 Adenohypophysis 
I. It is also called 
glandular lobe. 
II. This is anterior two 
third of the gland 
which is formed 
from stomodeal 
portion. 
III. It has following 
parts 
Pars distalis, Pars 
tuberalis, Pars 
intermedia 
 Neurohypophysis 
I. This is formed from 
neural ectoderm. 
II. It has following parts: 
Pars 
nervosa,Infundibulum.
Chromophils 
These are ovoid cells with granular cytoplasm 
nuclei.They take up acid or basic stains and are 
classified into two types 
a. Acidophils ( alpha cells):- 
Acidophils are rounded or ovoid cells 10 to 20 
micron have cytoplasm that stains red or orange 
b. Acidophils are of two types 
• Somatotrophs:- These cells secrete 
somatotropin hormones and growth 
hormaones 
• Mammotrophs( leutotrophs or prolactin cells):- 
These cells secrete prolactin hormone.
Adinohypophysis 
 Pars distalis:-Pars distalis or anterior lobe is the 
largest(75%)part of pituitory gland supported by 
collagenous capsule. 
 In this region ,two major types of cells occur 
namely chromophils & chromophobes .
 Basophils( beta cells):- 
 They are irregular shaped and stain with basic 
dyes. 
Basophils are of two types 
 i. Thyrotrophs :- These cells secrete thyrotrophic 
hormone 
 ii. Gonadotrophs :- These cells secrete 
gonadotrophic hormone .i.e. FSH ( Follicle 
stimulating hormone ) and LH ( leutinizing 
Hormone)
Chromophobes (chief cells) 
 These cells are spherical ,elongated and irregular 
cells and appear in groups near center of cords 
 These cells contain light staining cytoplasm. 
 These cells produce adrenocorticotropic 
hormone(ACTH)
Pars tuberalis 
Pars tuberalis is a layer of cuboidal cells that 
covers the part of stalk and tuberal area of the 
brain. 
It is highly vascular. 
Not known whether these cells produce any 
hormon or not.
Pars Intermedia 
 It is a narrow region between pars distalis and 
pras nervosa. 
 It contains a stratum of epithelial cells which is 
formed by several layers of polygonal cells and 
they are basophilic. 
 The pars intermedia secrete melanonocytye 
stimulating hormone in lower vertebrates like fish 
and amphibians ,but in the mammals the function 
of MSH is obscure.
Fig
Neurohypophysis 
 It is formed by ventral cylindrical outgrowth from 
the floor of the diencephalon,which forms the 
stalk and posterior 1/3 of the pituitory gland. 
 The posterior 1/3 of the gland ,whioch is formed 
by swollen end of ventral outgrwoth, is called pars 
nervosa or infundibular process. 
 The cells of neural lobe are called pituicytes. 
 Two hormones Oxytoxin and Anti-diuretic are 
secreted from neurohypophysis.
Thank you……

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Histological structure of pituitory gland

  • 1. Histological Structure of Pituitary gland Asst. Prof Chetana Kanekar M.Sc ( SET life sciences)
  • 2. Introduction  The pituitory gland or hypophysis is structurally and functionally most complex endocrine gland.  Located in th ebrain attached by slender hypophysial or infundibular stalk,just behind the optic chiasma.  The pituitory gland has double origin as it is partly derived from oral ectoderm and partly from the brain.  The pituitory gland has two main parts: I. Adenohypophysis II. Neurohypophysis
  • 3.
  • 4.  Adenohypophysis I. It is also called glandular lobe. II. This is anterior two third of the gland which is formed from stomodeal portion. III. It has following parts Pars distalis, Pars tuberalis, Pars intermedia  Neurohypophysis I. This is formed from neural ectoderm. II. It has following parts: Pars nervosa,Infundibulum.
  • 5. Chromophils These are ovoid cells with granular cytoplasm nuclei.They take up acid or basic stains and are classified into two types a. Acidophils ( alpha cells):- Acidophils are rounded or ovoid cells 10 to 20 micron have cytoplasm that stains red or orange b. Acidophils are of two types • Somatotrophs:- These cells secrete somatotropin hormones and growth hormaones • Mammotrophs( leutotrophs or prolactin cells):- These cells secrete prolactin hormone.
  • 6. Adinohypophysis  Pars distalis:-Pars distalis or anterior lobe is the largest(75%)part of pituitory gland supported by collagenous capsule.  In this region ,two major types of cells occur namely chromophils & chromophobes .
  • 7.  Basophils( beta cells):-  They are irregular shaped and stain with basic dyes. Basophils are of two types  i. Thyrotrophs :- These cells secrete thyrotrophic hormone  ii. Gonadotrophs :- These cells secrete gonadotrophic hormone .i.e. FSH ( Follicle stimulating hormone ) and LH ( leutinizing Hormone)
  • 8. Chromophobes (chief cells)  These cells are spherical ,elongated and irregular cells and appear in groups near center of cords  These cells contain light staining cytoplasm.  These cells produce adrenocorticotropic hormone(ACTH)
  • 9. Pars tuberalis Pars tuberalis is a layer of cuboidal cells that covers the part of stalk and tuberal area of the brain. It is highly vascular. Not known whether these cells produce any hormon or not.
  • 10. Pars Intermedia  It is a narrow region between pars distalis and pras nervosa.  It contains a stratum of epithelial cells which is formed by several layers of polygonal cells and they are basophilic.  The pars intermedia secrete melanonocytye stimulating hormone in lower vertebrates like fish and amphibians ,but in the mammals the function of MSH is obscure.
  • 11. Fig
  • 12. Neurohypophysis  It is formed by ventral cylindrical outgrowth from the floor of the diencephalon,which forms the stalk and posterior 1/3 of the pituitory gland.  The posterior 1/3 of the gland ,whioch is formed by swollen end of ventral outgrwoth, is called pars nervosa or infundibular process.  The cells of neural lobe are called pituicytes.  Two hormones Oxytoxin and Anti-diuretic are secreted from neurohypophysis.