Glycos Biotechnologies is developing technologies to produce biochemicals through microbial fermentation of renewable feedstocks. The company is focusing on producing isoprene through genetically modified E. coli that can convert glycerol and fatty acids into isoprene. The document discusses an ongoing project to control cell growth by deleting the gltA gene in the TCA cycle. It provides details on the deletion methods, experimental results showing growth on different supplements, and reactor experiments testing isoprene production.
This document describes the development of composite polymer scaffolds made of PLGA/PEG particles combined with hydrogel components like Pluronic F127, fibrin, or hyaluronic acid. The scaffolds are formed by mixing the PLGA/PEG particles with a hydrogel at room temperature, then allowing them to solidify at 37°C as the particles sinter together over time. Testing showed the compressive strength of the scaffolds increased between 15 minutes and 2 hours at 37°C. The maximum strengths were 1.2 MPa for PLGA/PEG-Pluronic F127 scaffolds, 2.4 MPa for PLGA/PEG-hyaluronic acid scaffolds, and 0.6 MPa for PLGA
Abstracts from sps laboratory for the national conference in biosciences, sau...Saurashtra University
One-Day National Conference on Innovations in Biological Sciences
On 10 January 2020 in the Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India
Abstracts of the papers presented in this conference by the members of Prof. Satya P. Singh Lab
Solubility Enhancement, Stability and Scalability of Mesoporous Silica Formul...MilliporeSigma
In these slides, you will be introduced to the science and scale-up behind mesoporous silica technology, an emerging formulation option for poorly soluble drug delivery.
Included in the slides:
- A broad overview of mesoporous silica technology
- An introduction to the unique stability advantages of mesoporous silica
- Case studies of in vitro and in vivo performance of mesoporous silica formulations
- How to scale-up from lab to production scale
Watch the webinar here: https://bit.ly/2IoV8k7
This document discusses research into synthesizing and characterizing isoprene hydroperoxides, which are atmospheric oxidation products of isoprene that can influence air quality and climate. The researchers developed a synthetic route to produce hydroperoxides via reactions of epoxides with peroxy nucleophiles. Preliminary results identified four potential hydroperoxide compounds via chromatography. However, only one, 3,4-isoprene hydroperoxide, was conclusively characterized with NMR. Future work will optimize purification methods and synthesize additional isoprene hydroperoxides to enable kinetic and gas phase studies of these compounds.
This document outlines the metabolic pathway of isoprene, which is a precursor of cholesterol and other steroids. It shows the enzymatic conversions from acetyl-CoA and acetoacetyl-CoA to mevalonate, and then to geranylgeranyl pyrophosphate. This compound is further metabolized through squalene and lanosterol to cholesterol, ergosterol, calciferol, and other steroids. The pathway also branches to produce carotenoids like beta-carotene and lycopene.
This document describes the development of composite polymer scaffolds made of PLGA/PEG particles combined with hydrogel components like Pluronic F127, fibrin, or hyaluronic acid. The scaffolds are formed by mixing the PLGA/PEG particles with a hydrogel at room temperature, then allowing them to solidify at 37°C as the particles sinter together over time. Testing showed the compressive strength of the scaffolds increased between 15 minutes and 2 hours at 37°C. The maximum strengths were 1.2 MPa for PLGA/PEG-Pluronic F127 scaffolds, 2.4 MPa for PLGA/PEG-hyaluronic acid scaffolds, and 0.6 MPa for PLGA
Abstracts from sps laboratory for the national conference in biosciences, sau...Saurashtra University
One-Day National Conference on Innovations in Biological Sciences
On 10 January 2020 in the Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India
Abstracts of the papers presented in this conference by the members of Prof. Satya P. Singh Lab
Solubility Enhancement, Stability and Scalability of Mesoporous Silica Formul...MilliporeSigma
In these slides, you will be introduced to the science and scale-up behind mesoporous silica technology, an emerging formulation option for poorly soluble drug delivery.
Included in the slides:
- A broad overview of mesoporous silica technology
- An introduction to the unique stability advantages of mesoporous silica
- Case studies of in vitro and in vivo performance of mesoporous silica formulations
- How to scale-up from lab to production scale
Watch the webinar here: https://bit.ly/2IoV8k7
This document discusses research into synthesizing and characterizing isoprene hydroperoxides, which are atmospheric oxidation products of isoprene that can influence air quality and climate. The researchers developed a synthetic route to produce hydroperoxides via reactions of epoxides with peroxy nucleophiles. Preliminary results identified four potential hydroperoxide compounds via chromatography. However, only one, 3,4-isoprene hydroperoxide, was conclusively characterized with NMR. Future work will optimize purification methods and synthesize additional isoprene hydroperoxides to enable kinetic and gas phase studies of these compounds.
This document outlines the metabolic pathway of isoprene, which is a precursor of cholesterol and other steroids. It shows the enzymatic conversions from acetyl-CoA and acetoacetyl-CoA to mevalonate, and then to geranylgeranyl pyrophosphate. This compound is further metabolized through squalene and lanosterol to cholesterol, ergosterol, calciferol, and other steroids. The pathway also branches to produce carotenoids like beta-carotene and lycopene.
The document discusses the isoprene biogenesis process and poses several open questions about its evolutionary origins and applications. It notes clues about bio-isoprene production and concludes by questioning how far interactions can be understood, whether information is ever-ending, the nature of evolutionary interactions, and how to extract and apply the enormous amount of available information.
The presentation discusses about oxidation of Isoprene, the most reactive organic compound. It is also the highest present non-methane organic compound in the atmosphere. Isoprene is produced by plants and is highly reactive in the atmosphere. The major destruction of this compound is by oxidation. The major oxidants, the products formed and their yields in the oxidation process is discussed in the presentation.
The presentation is done as part of graduate coursework at University of Florida. The author studied master's in environmental engineering sciences during the making of the presentation.
Industrial Biotechnology-Sustainable Biorefineries - Richard LaDuca - Genenco...Burton Lee
Industrial biotechnology uses enzymes and engineered microorganisms to convert renewable biomass into fuels, power, and chemicals. This process is analogous to petroleum refineries and enables the development of biorefineries. Genencor is a leader in industrial biotechnology and has developed enzymes that enable the conversion of starch and cellulosic feedstocks into biofuels and biochemicals. Genencor's enzymes have helped advance biorefineries from first generation starch-based ethanol to future generations using lignocellulosic biomass as a sustainable feedstock.
- In 1887, Wallach enunciated the isoprene rule, which states that the skeleton structures of all naturally occurring terpenoids are built up of isoprene units.
- Isoprene, with the empirical formula C5H8, is the monomeric structural unit that all terpenoids are derived from and thus called isoprenoid compounds.
- Ingold formulated the special isoprene rule in 1925 to describe how the isoprene units in terpenoids are linked together in a head-to-tail fashion, with the branched end considered the head and the other end the tail.
This document discusses isoprenoids, which are compounds derived from isopentenyl pyrophosphate (IPP). It summarizes that isoprenoids play important roles in animals, plants, and modern life. It also describes a method developed by Keller and Thompson to efficiently synthesize isoprenoid pyrophosphate intermediates using bis(triethylammonium) phosphate and trichloroacetonitrile, followed by silica chromatography. This procedure allows rapid preparation of isoprenoid pyrophosphates that are widely used in studies of isoprenoid metabolism.
BioIsoprene-BioChemicals and BioFuels Applications - Gregory Bohlmann - Genen...Burton Lee
This document discusses Genencor's BioIsopreneTM platform for producing branched chain hydrocarbons from biomass for use in biofuels and biochemical applications. Genencor has developed a process using recombinant DNA and gas phase fermentation to produce the C5 hydrocarbon isoprene from biomass. Isoprene and its derivatives C10 and C15 can be used as drop-in fuels with energy content comparable to gasoline and diesel and an 80% reduction in greenhouse gas emissions over petroleum-based fuels. The BioIsopreneTM platform enables a biorefinery approach to producing both fuels and biochemicals from renewable feedstocks.
Natural rubber is a natural polymer obtained from the latex of the rubber tree Hevea brasiliensis. It is composed of polyisoprene, which is a long chain of the monomer isoprene. Latex is a white fluid that is coagulated to form solid natural rubber through the addition of bacteria or acids that lower the pH. Vulcanization improves the properties of natural rubber by adding sulfur to form cross-links between polymer chains, making the rubber more durable and elastic.
This presentation provides an overview of different types of biofuels. First generation biofuels are made from sugars and vegetable oils, while second generation biofuels can be made from various biomass sources like cellulosic ethanol from algae or wood. Specific biofuels discussed include bioethanol, biomethanol, biobutanol, biodiesel, green diesel, biofuel gasoline, vegetable oils, bioethers, biogas, and solid biofuels. Advantages are reduced reliance on foreign oil and reduced pollution, while disadvantages include potential rises in food prices, vehicle safety concerns, and issues with energy balance. Biofuels can be used as alternatives to fossil fuels for transportation, heating homes, and
Terpenes are natural products made of isoprene units. This document discusses different classes of terpenes including monoterpenes, sesquiterpenes, and triterpenes which are classified based on the number of isoprene units. It also describes the biosynthesis pathways and methods for identification of triterpenes including NMR spectroscopy, thin layer chromatography with various spray reagents, and isolation from plant sources. Four new triterpene compounds isolated from green tea and two new compounds from other plants are characterized based on spectral data and chemical properties.
1) Terpenes are derived from head-to-tail polymerization of isoprene units (C5). Monoterpenes contain 2 units, sesquiterpenes contain 3 units, and so on.
2) Mevalonic acid is converted to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), the basic C5 units. Repeated condensation of these units leads to prenyl diphosphates of different sizes.
3) These prenyl diphosphates are converted by terpene synthases into the skeletons of various terpenes, which may then undergo further modifications. This
Shikimic acid is an important biochemical metabolite found in plants and microorganisms. It was first isolated from the Japanese star anise plant in 1885. The shikimate pathway is a seven step metabolic route used by plants and microbes to synthesize the aromatic amino acids phenylalanine, tyrosine, and tryptophan. This pathway is not present in animals. Shikimic acid is the starting point for the biosynthesis of many important phenolic compounds like flavonoids, tannins, lignin, and gallic acid. It is also a precursor for indole derivatives and the psychedelic compound DMT. Glyphosate herbicide works by inhibiting the shikimate pathway in plants
Plants produce a vast and diverse organic compounds, which do not appear to participate directly in growth and development.These substances traditionally referred to as secondary metabolites which terpenes are one of them.
1. Secondary metabolites are molecules produced by organisms that are not essential for growth but provide other important functions.
2. Alkaloids are an important class of secondary metabolites derived from amino acids. They have diverse pharmacological effects used in medicine.
3. Terpenoids are another major class of secondary metabolites derived from chains of isoprene units. They contribute flavors, scents, pigments and hormones in plants.
Enron Scandal from Auditor's Perspective (F-310) Pantho Sarker
This presentation is basically on the Enron's scandal. Here the maximum focus is given to auditor's role in this fraud. Moreover, the management's role is also highlighted.
Organization Structure & Corporate Social ResponsibilityPantho Sarker
In this presentation the organizational structure and corporate social responsibilities (CSR) of Central Insurance Company Limited is is shown and analysed.
This document is a presentation on monetary policy in Bangladesh by Group 16. It begins with introductions of the group members. The presentation covers topics such as the definition of monetary policy, the tools and transmission mechanisms of monetary policy, impacts of monetary policy on inflation and capital markets, Bangladesh Bank's monetary policy stances and challenges to monetary policy in Bangladesh. The presentation provides an overview of key concepts in monetary policy as well as analysis of monetary policies implemented in Bangladesh.
Hydrolysate Characterization Technical Presentation Webinar11 2009Mason Williams
1) The document discusses developing a chemically defined hydrolysate alternative to mitigate risks associated with undefined natural hydrolysates.
2) The approach involves fractionating multiple hydrolysates using RP-HPLC and identifying bioactive fractions that stimulate cell growth and protein production in CHO cells.
3) A new product called EX-CELL CD Hydrolysate Fusion was developed that contains synthetic components identified from bioactive fractions and provides 80-100% of the performance of natural hydrolysates.
The document discusses several current projects involving collagen and its applications. It describes developing a collagen-hydroxyapatite scaffold for bone defects, analyzing its pore size and efficacy. It also mentions exploring collagen-silver nanoparticle composites for wound care and using collagen coatings for tissue culture. Developing a hemostatic material using collagen and kaolin for heavy bleeding is also summarized.
- The document summarizes the MSc thesis of Mauro José Castanho Claudino which aimed to screen and characterize different immobilization methods for whole-cell steroid bioconversion using Mycobacterium sp. NRRL B-3805.
- Silicone was found to be the most suitable immobilization support, providing efficient cell adsorption, good thermal stability, and ability to catalyze the biotransformation of β-sitosterol to 4-androstene-3,17-dione over multiple batches. Kinetic studies showed Michaelis-Menten behavior and storage stability could be modeled using bi-exponential equations.
- The work demonstrated the feasibility of using small-scale bioreactors to
The document provides information about biomanufacturing education and training programs. It discusses the Northeast Biomanufacturing Collaborative and Center's work since 2003 to define the skills, knowledge, and abilities needed by biomanufacturing technicians. It highlights the development of curriculum and instructional materials to educate technicians for careers supporting the bioeconomy. Examples are given of various biomanufacturing processes and the equipment involved in areas like biopharmaceuticals, biofuels, and industrial biotechnology.
The document discusses the isoprene biogenesis process and poses several open questions about its evolutionary origins and applications. It notes clues about bio-isoprene production and concludes by questioning how far interactions can be understood, whether information is ever-ending, the nature of evolutionary interactions, and how to extract and apply the enormous amount of available information.
The presentation discusses about oxidation of Isoprene, the most reactive organic compound. It is also the highest present non-methane organic compound in the atmosphere. Isoprene is produced by plants and is highly reactive in the atmosphere. The major destruction of this compound is by oxidation. The major oxidants, the products formed and their yields in the oxidation process is discussed in the presentation.
The presentation is done as part of graduate coursework at University of Florida. The author studied master's in environmental engineering sciences during the making of the presentation.
Industrial Biotechnology-Sustainable Biorefineries - Richard LaDuca - Genenco...Burton Lee
Industrial biotechnology uses enzymes and engineered microorganisms to convert renewable biomass into fuels, power, and chemicals. This process is analogous to petroleum refineries and enables the development of biorefineries. Genencor is a leader in industrial biotechnology and has developed enzymes that enable the conversion of starch and cellulosic feedstocks into biofuels and biochemicals. Genencor's enzymes have helped advance biorefineries from first generation starch-based ethanol to future generations using lignocellulosic biomass as a sustainable feedstock.
- In 1887, Wallach enunciated the isoprene rule, which states that the skeleton structures of all naturally occurring terpenoids are built up of isoprene units.
- Isoprene, with the empirical formula C5H8, is the monomeric structural unit that all terpenoids are derived from and thus called isoprenoid compounds.
- Ingold formulated the special isoprene rule in 1925 to describe how the isoprene units in terpenoids are linked together in a head-to-tail fashion, with the branched end considered the head and the other end the tail.
This document discusses isoprenoids, which are compounds derived from isopentenyl pyrophosphate (IPP). It summarizes that isoprenoids play important roles in animals, plants, and modern life. It also describes a method developed by Keller and Thompson to efficiently synthesize isoprenoid pyrophosphate intermediates using bis(triethylammonium) phosphate and trichloroacetonitrile, followed by silica chromatography. This procedure allows rapid preparation of isoprenoid pyrophosphates that are widely used in studies of isoprenoid metabolism.
BioIsoprene-BioChemicals and BioFuels Applications - Gregory Bohlmann - Genen...Burton Lee
This document discusses Genencor's BioIsopreneTM platform for producing branched chain hydrocarbons from biomass for use in biofuels and biochemical applications. Genencor has developed a process using recombinant DNA and gas phase fermentation to produce the C5 hydrocarbon isoprene from biomass. Isoprene and its derivatives C10 and C15 can be used as drop-in fuels with energy content comparable to gasoline and diesel and an 80% reduction in greenhouse gas emissions over petroleum-based fuels. The BioIsopreneTM platform enables a biorefinery approach to producing both fuels and biochemicals from renewable feedstocks.
Natural rubber is a natural polymer obtained from the latex of the rubber tree Hevea brasiliensis. It is composed of polyisoprene, which is a long chain of the monomer isoprene. Latex is a white fluid that is coagulated to form solid natural rubber through the addition of bacteria or acids that lower the pH. Vulcanization improves the properties of natural rubber by adding sulfur to form cross-links between polymer chains, making the rubber more durable and elastic.
This presentation provides an overview of different types of biofuels. First generation biofuels are made from sugars and vegetable oils, while second generation biofuels can be made from various biomass sources like cellulosic ethanol from algae or wood. Specific biofuels discussed include bioethanol, biomethanol, biobutanol, biodiesel, green diesel, biofuel gasoline, vegetable oils, bioethers, biogas, and solid biofuels. Advantages are reduced reliance on foreign oil and reduced pollution, while disadvantages include potential rises in food prices, vehicle safety concerns, and issues with energy balance. Biofuels can be used as alternatives to fossil fuels for transportation, heating homes, and
Terpenes are natural products made of isoprene units. This document discusses different classes of terpenes including monoterpenes, sesquiterpenes, and triterpenes which are classified based on the number of isoprene units. It also describes the biosynthesis pathways and methods for identification of triterpenes including NMR spectroscopy, thin layer chromatography with various spray reagents, and isolation from plant sources. Four new triterpene compounds isolated from green tea and two new compounds from other plants are characterized based on spectral data and chemical properties.
1) Terpenes are derived from head-to-tail polymerization of isoprene units (C5). Monoterpenes contain 2 units, sesquiterpenes contain 3 units, and so on.
2) Mevalonic acid is converted to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), the basic C5 units. Repeated condensation of these units leads to prenyl diphosphates of different sizes.
3) These prenyl diphosphates are converted by terpene synthases into the skeletons of various terpenes, which may then undergo further modifications. This
Shikimic acid is an important biochemical metabolite found in plants and microorganisms. It was first isolated from the Japanese star anise plant in 1885. The shikimate pathway is a seven step metabolic route used by plants and microbes to synthesize the aromatic amino acids phenylalanine, tyrosine, and tryptophan. This pathway is not present in animals. Shikimic acid is the starting point for the biosynthesis of many important phenolic compounds like flavonoids, tannins, lignin, and gallic acid. It is also a precursor for indole derivatives and the psychedelic compound DMT. Glyphosate herbicide works by inhibiting the shikimate pathway in plants
Plants produce a vast and diverse organic compounds, which do not appear to participate directly in growth and development.These substances traditionally referred to as secondary metabolites which terpenes are one of them.
1. Secondary metabolites are molecules produced by organisms that are not essential for growth but provide other important functions.
2. Alkaloids are an important class of secondary metabolites derived from amino acids. They have diverse pharmacological effects used in medicine.
3. Terpenoids are another major class of secondary metabolites derived from chains of isoprene units. They contribute flavors, scents, pigments and hormones in plants.
Enron Scandal from Auditor's Perspective (F-310) Pantho Sarker
This presentation is basically on the Enron's scandal. Here the maximum focus is given to auditor's role in this fraud. Moreover, the management's role is also highlighted.
Organization Structure & Corporate Social ResponsibilityPantho Sarker
In this presentation the organizational structure and corporate social responsibilities (CSR) of Central Insurance Company Limited is is shown and analysed.
This document is a presentation on monetary policy in Bangladesh by Group 16. It begins with introductions of the group members. The presentation covers topics such as the definition of monetary policy, the tools and transmission mechanisms of monetary policy, impacts of monetary policy on inflation and capital markets, Bangladesh Bank's monetary policy stances and challenges to monetary policy in Bangladesh. The presentation provides an overview of key concepts in monetary policy as well as analysis of monetary policies implemented in Bangladesh.
Hydrolysate Characterization Technical Presentation Webinar11 2009Mason Williams
1) The document discusses developing a chemically defined hydrolysate alternative to mitigate risks associated with undefined natural hydrolysates.
2) The approach involves fractionating multiple hydrolysates using RP-HPLC and identifying bioactive fractions that stimulate cell growth and protein production in CHO cells.
3) A new product called EX-CELL CD Hydrolysate Fusion was developed that contains synthetic components identified from bioactive fractions and provides 80-100% of the performance of natural hydrolysates.
The document discusses several current projects involving collagen and its applications. It describes developing a collagen-hydroxyapatite scaffold for bone defects, analyzing its pore size and efficacy. It also mentions exploring collagen-silver nanoparticle composites for wound care and using collagen coatings for tissue culture. Developing a hemostatic material using collagen and kaolin for heavy bleeding is also summarized.
- The document summarizes the MSc thesis of Mauro José Castanho Claudino which aimed to screen and characterize different immobilization methods for whole-cell steroid bioconversion using Mycobacterium sp. NRRL B-3805.
- Silicone was found to be the most suitable immobilization support, providing efficient cell adsorption, good thermal stability, and ability to catalyze the biotransformation of β-sitosterol to 4-androstene-3,17-dione over multiple batches. Kinetic studies showed Michaelis-Menten behavior and storage stability could be modeled using bi-exponential equations.
- The work demonstrated the feasibility of using small-scale bioreactors to
The document provides information about biomanufacturing education and training programs. It discusses the Northeast Biomanufacturing Collaborative and Center's work since 2003 to define the skills, knowledge, and abilities needed by biomanufacturing technicians. It highlights the development of curriculum and instructional materials to educate technicians for careers supporting the bioeconomy. Examples are given of various biomanufacturing processes and the equipment involved in areas like biopharmaceuticals, biofuels, and industrial biotechnology.
JBEI Research Highlights - November 2017 Irina Silva
This document summarizes the development and testing of a quorum sensing (QS)-mediated gene expression system to control bisabolene production in engineered E. coli. Researchers developed a QS system using the LuxI/R genes from Vibrio fischeri to induce expression of the bisabolene production pathway without the need for external inducers. The best QS strain, with the sensor genes integrated into the genome and an optimized response plasmid, produced 1.1 g/L of bisabolene, a 44% improvement over previous inducible systems. This QS-based system provided defined and homogeneous gene expression and production compared to inducible controls.
An Industrial Method of Biofuel Production from ChitinCharlie Pei
This document summarizes research on developing an industrial process for producing biofuels from chitin, the second most abundant natural biopolymer. The process involves three main steps: 1) pretreating and hydrolyzing chitin to release glucosamine (GlcN), 2) fermenting GlcN using bacteria to produce biofuels, and 3) modeling an industrial plant using the experimental data. Several bacterial species were tested for GlcN fermentation, and Clostridium acetobutylicum ATCC 55025 produced the highest butanol yield. Optimization of pretreatment, hydrolysis and fermentation conditions improved GlcN yields and biofuel production. An economic model of an industrial
Recombinant protein expression and purification Lecturetest
The document discusses recombinant protein expression and engineering. It describes:
1) Cloning or synthesizing the gene of interest, making an expression construct, transfecting cells, purifying the recombinant protein.
2) Factors to consider like the protein's origin (prokaryotic/eukaryotic), required post-translational modifications, and available expression systems.
3) A case study expressing recombinant human alpha-1-acid glycoprotein in E. coli, including vector construction, periplasmic extraction, affinity purification, and yield.
The study analyzed the effect of common poultry medicines on ethanol fermentation using Saccharomyces cerevisiae. Various concentrations of Avatec, Cygro, Robenz, and Monesine were tested in batch reactions at 33°C and 36°C. Results showed that higher concentrations of Robenz and Avatec decreased sucrose concentration over time, suggesting reduced bacterial contaminants and increased yeast growth. Specifically, Robenz 100 ppm/L and Avatec 1000 ppm/L at 33°C, and Avatec 100 ppm/L at 36°C minimized bacterial growth. However, very high medicine concentrations also reduced yeast. Further analysis is needed to differentiate sucrose consumption by yeast versus bacteria to
A study with enzymatic membrane reactor for conversion of lactose in to galac...Pallavi Kumari
The formation of galacto-oligosaccharides (GOS) from lactose by commercially available Biolacta FN5 (β- galactosidase, EC 3.2.1.23) derived from Bacillus circulans was studied under immobilized enzyme condition. The present work utilizes hydrophobic membrane (0.22 m pore size) for immobilization of enzyme. Experiments were conducted in a three compartment cell. The middle compartment (~25 mL) being separated by immobilized membranes was utilized for feed lactose solution; whereas, adjacent compartments were filled with distilled water. The reacted mixture solution was analyzed for tri-, tetra- and penta- forms of GOS which depended on varying amounts of initial lactose (ILC) and enzyme concentrations. Total GOS formation increased from 7 to 28% for ILC from 50 to 200 g/L. However, tri-saccharide was the major (67%) in comparison to tetra (27%) and penta (6%) forms of GOS. There was marginal difference of GOS formations while comparing the result (GOS yield) under both free (~30%) and immobilized (~28%) conditions.
Structural Studies of Human GBE1 and Relevance to APBDBen Decker
The document summarizes Wyatt Yue's work studying the structure of human GBE1 and its relevance to Adult Polyglucosan Body Disease (APBD). Key points:
1. Wyatt determined the crystal structures of human GBE1 in its apo form and bound to acarbose and Glc7, to understand its shape and function.
2. Mutations in GBE1 cause APBD. Wyatt purified several GBE1 mutants to characterize, including Y329S, and determined the Y329 site is disrupted in the mutant structure.
3. Wyatt collaborates with APBD patient groups like APBDRF to translate structural findings into potential pharmacological chaperone treatments for
This document discusses several papers that apply machine learning and multi-omics data to predict metabolic pathway dynamics.
- One paper develops a machine learning approach using proteomics data instead of kinetic modeling to accurately predict metabolite concentrations. This approach provides faster development of predictive models since it infers knowledge from data rather than requiring domain expertise.
- Another paper applies machine learning and multi-omics data to quantitatively predict production of the biofuel isopentenol from limited training data.
- A third paper reviews how knowledge engineering and data-driven frameworks using machine learning can offer new constraints for mechanistic models to better describe cellular regulation and design metabolic pathways. This facilitates "learn and design" for strain development.
JBEI Research Highlights - October 2021SaraHarmon4
This document summarizes a study that evaluated the potential for nutrient recovery from wet organic waste processing facilities in California to offset synthetic fertilizer demand. The study found that recovering nitrogen and phosphorus from organic waste streams through anaerobic digestion and separation techniques could meet 11% of the state's nitrogen and 29% of phosphorus fertilizer needs. Recovered nutrients would be in the form of liquid fertilizer, struvite, and compost. The approach provides a foundation for analyzing national-level nutrient flows and recovery potentials from bioenergy production.
This document summarizes research optimizing zeaxanthin production using immobilized Flavobacterium sp. cells in a fluidized bed bioreactor. The researchers conducted experiments to determine the best conditions for zeaxanthin production, including culture medium composition and bioreactor parameters. An orthogonal experimental design was used to evaluate factors like aeration, pH, pellet size, and carbon-nitrogen ratio. Maximum zeaxanthin production of 3.16 g/L was achieved under optimal conditions, approximately 10 times greater than previous reports. Dimensionless numbers were also correlated to describe process behavior and enable scale-up.
Solubility Enhancement, Stability and Scalability of Mesoporous Silica Formul...Merck Life Sciences
In these slides, you will be introduced to the science and scale-up behind mesoporous silica technology, an emerging formulation option for poorly soluble drug delivery.
Included in the slides:
- A broad overview of mesoporous silica technology
- An introduction to the unique stability advantages of mesoporous silica
- Case studies of in vitro and in vivo performance of mesoporous silica formulations
- How to scale-up from lab to production scale
Watch the webinar here: https://bit.ly/2IoV8k7
This document summarizes an experiment to improve the production of glycolic acid (GA) through the bioconversion of ethylene glycol (EG) using Gluconobacter oxydans. The researchers tested three techniques: fed-batch catalysis (FBC), continuous feeding catalysis (CFC), and successive recycled-cell catalysis (SRC). SRC involved recycling the bacterial cells every 48 hours over five cycles, achieving a total GA production of 490.7 g with over 90% yield and average productivity of 2.04 g/L/h, significantly improving upon FBC and CFC. The twin strategies of end-product control and cell recycling successfully demonstrated large-scale bioconversion of EG to GA.
Potassium sorbate, sodium benzoate and citric acid together with vacuum packing have extended puree shelf life by 3-6 months in our trials at ambient conditions (T<25C).
However, the gap lies on the efficacy of the preservative combination in retarding or stopping the growth of harmful pathogens in the puree, and also their effects on β-carotene retention is unknown.
Biological Systems: A Special Case
Up till now we have discussed various aspects of the separation and processing of fine solids without too much reference (except in the examples) to the specifics of the properties of the materials concerned. Though the material properties are the dominant influence on efficient process design and operation, it has been postulated that the necessary characteristics for process selection and optimization can be found fairly readily using easily-applicable rheological and other techniques. This underlying assumption also seems to hold good for biological suspensions; however, certain aspects of the behavior of these systems are sufficiently specialized for them to merit a separate discussion viz:
1 TYPES OF BIOLOGICAL SEPARATION
1.1 Whole-Organism Case
1.2 Part-Cell Separations
1.3 Isolation of Individual Molecular Species
2 SETTING ABOUT DEVISING AN EFFECTIVE
PROCESS FOR SEPARATION OF A BIOLOGICAL MATERIAL
2.1 Whole-Organism Case
2.1.1 Characterization of Biopolymers in the Liquor
2.1.2 Release of Internal Water
2.2 Part -Cell Separations
2.2.1 Selectivity
2.2.2 Cost
2.3 Isolation of Individual Molecular Species
3 Examples
3.1 Effective Design and Operation of a Process for Harvesting of Single Cell Protein
3.2 Harvesting of Mycoprotein for Human Consumption
3.3 Thickening of a Filamentous Organism Suspension
3.4 Separation of Poly-3-hydroxybutyrate Polymer (PHB) from Alcaligenes Eutrophus Biomass
3.5 Isolation of Organic Acid Produced by an Enzymatic Process
4 REFERENCES
Table
Figures
This study establishs a basis for growing
plantlets without sucrose and investigating other
factors like carbon dioxide and light regime to
improve the in vitro growth performance.
β-galactosidase (β-D-galactoside galacto hydrolase, E.C.3.2.1.23, trivially lactate) known primarily as a hydrolase enzyme. β-galactosidase generally hydrolysis of lactose into glucose and galactose.
β-galactosidase
Lactose → Galactose + Glucose
Similar to Glycos Biotechnologies.TAMU presentation_PC edits (20)
2. Presentation Outline
Nothing in this presentation is confidential, all has been cleared as public information
• GlycosBio
Purpose of the company
What they produce and why
Company components
• Project – controlling cell growth by modifying theTCA Cycle
Importance of gltA deletion
Methods of gltA deletion
Fermentation testing of deletion
3. Company Mission
Create high value biochemicals through microbial
fermentation using sustainable, renewable, non-food based
feed stocks
9. Richard Cilento
Chief Executive
Officer
Walter Burnap
President, Chief
Financial Officer
Kevin Mitchell
VP Finance
Diane Muniz-
Chong Exec.
Assistant
Janel Chitty
Exec. Assistant
Accounting
Donna Muniz
Accountant
Paul
Campbell
Co-Founder,
Chief Science
Officer
Werner
Bussmann
VP Project
Management
David Gaskin
Project
Manager
Shadab
Mohommed
Process Engineer
Alex Reis
Engineering
Intern
Dan Monticello
VP Research &
Development
Matt
Wong
RS
Munira
Momin
RA
Robert
West
RS
Sailandra
Paude
RA
Kristian
Odfalk
Intern
Mai Li
RS
Kimberly
Marroquin
Intern
Stephanie
Doneske
RS
Cindy
Austin
RA
Josh
Munnerlyn
RA
Huajin
Zhou
RS
Allana
Robertson
Intern
Sebastian
Bedrow
RS
Erin Burke
RS
Katherine
Walton
RA
Ivy
Martinez
RA
Ryan Black
Manager of
Process
Economics
Solutions Support
Team
AnalyticalMolecular
Genetics
FermentationEngineeringHuman
Resources
Business
Development
11. Business Development
▪ CEO-heads effort but is assisted by theVP Finance; CFO; and Chief Science
Officer.
▪ Together they make up the face of the company
▪ Three main goals are:
1.CreateValue
– Find your market, find you niche within market
2. Raise Funds/ Inv.
– Raises money through venture capitalism; angel investments
– Investment firms; Individual investors
3. Build Partnerships
– Feedstock Suppliers
– Buyers of Final Product
13. Research & Development
Strain Development
Fermentation Development
Product Capture & Analysis
▪ DevelopsTechnology
– Makes up 80% of current staff
– Headed by Chief Science Officer
andVice President of Research &
Development
15. Engineering
Responsible for:
▪ Scale Up Process
– Run experiments to determine strain constraints i.e. growth rates, flow rates, oxygen
demand, and product per liter of media
– Determine optimum media mixing and feeding process according to calculated demands
(Take feed stock and turn it into a usable form)
▪ Product Capture
– Determine process to turn gas phase isoprene into a liquid (transportable form)
▪ Plant Design
– Build and design production blueprint for collecting isoprene
– Determine optimum equipment sizing according to bacterial and material limitations
– Find businesses equipped to build system components according to specifications. (Process
requires pharmaceutical, fermentation, and chemical industry components)
– Recycle waste water from production process to reduce plant foot print
– Find the most economical way to produce isoprene and maximize returns
16. Attenuated TCA Cycle Project
Problem:We need a method to control cell growth through nutrient
limitation
Hypothesis: Would deleting gltA shut down the movement of carbon into the
TCA cycle, and eliminate cell growth, and if so could carbon source
supplementation restore cell growth?
▪ Molecular
– Why deleted - metabolic diagram
– How deleted- phage/suicide vector
▪ Fermentation
– Purpose-validate deletion
– Verify phenotype
19. Upper/Lower Mevalonate PathwayAcetyl CoA
Acetyl CoA
AcetoAcetyl CoA
3-hydroxyl-3-methylglutaryl-CoA
Acetyl CoA
atoB
HMGS
Mevalonate
2 NADH
2 NAD+
Mevalonate-P
ATP ADP
MEK PMK
Mevalonate-PP
ATP ADP
MPD
Isopentenyl-PP DMAPP
OHLDI
Isoprene
Upper
MEV
Lower
MEV
20. Deletion Methods
Deletion
Method
Time
Range
Chances
of
Success
Special
Construct
Accuracy Scar
Risk of Causing
Contamination
Cause of
Failure
Phage
Method
1-2 weeks
or longer
High No
(donor
strain
required)
Ok
(may cause
other
changes to
chromosome)
Yes Very High Strain gains
resistance to
P1 phage
Suicide
Vector
2 weeks or
longer
Good
(may be
hard to
resolve
backbone)
Yes
(design a
plasmid
that can
not be
replicated)
High No Low Tet resistant
mutation
makesTSS
counter
selection not
effective
21. Fig1
Streips U. N.,Yasbin R. E., Transduction in Gram-Negative Bacteria. 2002. Modern MicrobialGenetics, (2) 561-564.
22. Metcalf W., Jiang W., Daniels L. L., Kim S. K., Haldimann A., and Wanner B. L., Conditionally Replicative and Conjugative
Plasmids Carrying lacZα for Cloning, Mutagenesis, and Allele Replacement in Bacteria. 1995. Academic Press, Inc. 35: 1-13.
Kan
28. gltA Keio Collection-Flask Fermentation
0
5
10
15
20
25
30
0 5 10 15 20 25 30
OD600
Hours
YE
Negative Control
YE 1g/l
YE 2g/l
YE 4g/l
YE 8g/l
YE 16g/l
Cells died/cells couldn’t grow—b/c alpha keto-glutamate couldn’t produce proline & alanine. So carried out yeast/case amino acids/glutamate supplement experiment.
MEK-Mevalonate kinase
PMK-phosphomevalonate kinase
MPD-mevalonate 5-pyrophosphate decarboxylase
IDI-isopentenyl diphosphate delta isomerase (DMAPP- Dimethylallyl pyrophosphate)
MboS-methylbutenol synthase
LDI-Linalool Dehydratase-Isomerase
Through experimental testing we optimized the enzymes to drive the production of DMAPP to Isoprene
Tet resistant mutation makes TSS (media containing Na4P2O7+Tet) counter selection not effective so backbone can’t be resolved
Explain: that 2 types of viral particles are made, one with P1 DNA and one with packaged donor DNA
Cloning plate on LB+Kan for replication of suicide plasmid->Scale up 30ml flask to increase DNA concentration & PCR clean-> transform into GB130.76&77->Grow on Tet6 plates-> select colonies grow on LB for 2 days-> select colonies grow on TSS plates-> select big colonies grow on LB+Kan; LB+Tet6; LB
Mention that both integrations can either produce wild type or deletion genotype
1-Hypothesis of this experiment is: 4 amino acids need to be added to allow a delta gltA strain to grow.
2-Hypothesis is: deleting gltA will stop cell growth
1 Hypothesis of this experiment is: 4 amino acids need to be added to allow a delta gltA strain to grow.
2 Hypothesis is: deleting gltA will stop cell growth
Don’t forget to mention that amino acids supplemented feed in via alpha-ketoglutarate