The document summarizes glycolysis, the metabolic pathway that converts glucose into pyruvate. It defines glycolysis as the sequence of reactions that converts glucose or glycogen to pyruvate or lactate with ATP production. The pathway occurs in the cytosol of cells and produces either lactate under anaerobic conditions or pyruvate under aerobic conditions. Glycolysis is an important pathway for ATP synthesis in tissues lacking mitochondria. It also lists the enzymes involved and divides the pathway into three phases: an energy investment phase, splitting phase, and energy generation phase. The net reaction shows glucose is converted to pyruvate, ATP, NADH, and water.

1. Jai Narain Vyas University
Zoology Department
M.Sc 2nd Semestar
2017
Presented By
Bharat Nogia

2. Glycolysis

3. What is glycolysis ?
• Glycolysis is defined the sequence of
reactions converting glucose (or glycogen) to
pyruvate or lactate , with production of ATP.
• The word is derived from the Greek word—
glycose - sweet or sugar
lysis - dissolution

4. Salient features
• Also know as (Embden-Meyerof pathway) .
• The enzymes of this pathway are present in the
cytosomal fraction cell.
• When glycolysis occur in anaerobic condition its end
product is become Lactate or When glycolysis occur
in aerobic condition its end product is become
pyruvate.
• It is major pathway for ATP synthesis in tissue
lacking mitochondria (erytrocytes , cones).

5. Enzyme
• Gibbs Free Energy Changes
 Rxn# Enzyme DG°'(kJ/mol) DG(kJ/mol)
 1 Hexokinase -16.7 -33.5
 Phosphogluco-isomerase +1.7 -2.5
 3 Phosphofructokinase -14.2 -22.2
 4 Aldolase +23.9 -1.3
 5 Triose phos. Isomerase +7.6 +2.5
 6 G-3-PDH +12.6 -3.4
 7 Phosphoglycerate kinase -37.6 +2.6
 8 Phosphoglycerate mutas +8.8 +1.6
 9 Enolase +3.4 -6.6
 10 Pyruvate kinase -62.8 -33.4

6. Reaction of glycolysis
• The pathway of glycolysis is divided into 3
distinct phages –
1 Energy investment phase
2 Splitting phase
3 Energy generation phase

7. 1 Energy investment phase

12. Net Reaction:
Glucose + 2NAD+ + 2 Pi + 2 ADP
= 2 pyruvate + 2 ATP + 2 NADH + 2
H2O