This document discusses nitrogen fixing genes in bacteria and cyanobacteria. It describes the nif gene which codes for proteins involved in fixing atmospheric nitrogen into a form available to plants. These genes are found in nitrogen fixing bacteria and cyanobacteria. It also discusses nodulin genes which encode proteins that establish root nodules and allow for symbiotic nitrogen fixation. These nitrogen fixing genes are classified into those involved in bacterial cell surfaces and those involved in nodulation. Location of these genes varies between fast and slow growing rhizobia.
This presentation focus on how can be develop of herbicides resistant plants, Role of herbicides resistant plant, action of herbicides in unusual plants and agronomic importance of herbicides resistant plants.
Don"t forget to like, share and download
Presented by- MD JAKIR HOSSAIN
Doctoral Research Scholar
Department of Agricultural Genetic Engineering ,
Faculty of Agricultural Sciences and Technologies,
Nigde Omer Halisdemir University, Turkey
E. Mail- mjakirbotru@gmail.com
An overview of the Agrobacterium-mediated gene transfer process. Moreover, studied different kinds of Agrobacterium species are involved in this mechanism.
Agrobacterium is a rod-shaped, Gram-negative bacteria found mostly in the soil. It is a plant pathogen that is responsible for causing crown gall disease in them. This bacteria is also known as the natural genetic engineer because of it's the ability to integrate its plasmid Gene into the plant genome.
Agrobacterium tumefaciens transfer of their genetic material T-DNA of Ti-plasmid into the plant cell: A: Agrobacterium tumefaciens; B: Agrobacterium genome; C: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genes; D: Plant cell
A Ti-Plasmid (tumor-inducing plasmid) is a ds, circular DNA that often, but not always. It's a piece of genetic equipment that transfers genetic material from bacterial cells means Agrobacterium tumefaciens into plant cells used to induce tumors in the plant. The Ti-plasmid is damage when Agrobacterium is grown above 28 °C. Such cured bacteria don't induce crown gall disease in the plant due to they are avirulent. The Ti-Plasmid are classified into two types on the basis of opine genes are present in T-DNA.
The Plasmid has 196 genes that code for 195 proteins. There is no one structural RNA. The plasmid is 206.479 nucleotides long. the GC content is 56% and 81% of the genetic material is coding genes.
The modification of this plasmid is a very important source in the production of transgenic plants.
The T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS).
In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognized by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. So that the T-DNA can integrate into the host genome.
We inoculate Agrobacterium containing our genes of interest, onto wounded plant tissue explants. The Agrobacterium then transfers the gene of interest into the DNA of the plant tissue.
This presentation focus on how can be develop of herbicides resistant plants, Role of herbicides resistant plant, action of herbicides in unusual plants and agronomic importance of herbicides resistant plants.
Don"t forget to like, share and download
Presented by- MD JAKIR HOSSAIN
Doctoral Research Scholar
Department of Agricultural Genetic Engineering ,
Faculty of Agricultural Sciences and Technologies,
Nigde Omer Halisdemir University, Turkey
E. Mail- mjakirbotru@gmail.com
An overview of the Agrobacterium-mediated gene transfer process. Moreover, studied different kinds of Agrobacterium species are involved in this mechanism.
Agrobacterium is a rod-shaped, Gram-negative bacteria found mostly in the soil. It is a plant pathogen that is responsible for causing crown gall disease in them. This bacteria is also known as the natural genetic engineer because of it's the ability to integrate its plasmid Gene into the plant genome.
Agrobacterium tumefaciens transfer of their genetic material T-DNA of Ti-plasmid into the plant cell: A: Agrobacterium tumefaciens; B: Agrobacterium genome; C: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genes; D: Plant cell
A Ti-Plasmid (tumor-inducing plasmid) is a ds, circular DNA that often, but not always. It's a piece of genetic equipment that transfers genetic material from bacterial cells means Agrobacterium tumefaciens into plant cells used to induce tumors in the plant. The Ti-plasmid is damage when Agrobacterium is grown above 28 °C. Such cured bacteria don't induce crown gall disease in the plant due to they are avirulent. The Ti-Plasmid are classified into two types on the basis of opine genes are present in T-DNA.
The Plasmid has 196 genes that code for 195 proteins. There is no one structural RNA. The plasmid is 206.479 nucleotides long. the GC content is 56% and 81% of the genetic material is coding genes.
The modification of this plasmid is a very important source in the production of transgenic plants.
The T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS).
In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognized by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. So that the T-DNA can integrate into the host genome.
We inoculate Agrobacterium containing our genes of interest, onto wounded plant tissue explants. The Agrobacterium then transfers the gene of interest into the DNA of the plant tissue.
1.What is plant tissue culture?
2.Production of virus free plants.
3.History.
4.Virus elimination by heat treatment.
5.Virus elimination by Meristem Tip culture.
6.Factor affecting virus eradication by Meristem Tip culture.
7.Chemotherapy.
8.Virus elimination through in vitro shoot-tip Grafting.
9.Virus Indexing.
10.Conclusion .
11.References .
The advances of modern plant technologies, especially genetically modified crops, are considered to be a substantial benefit to agriculture and society. However, so-called transgene escape remains and is of environmental and regulatory concern. Genetic use restriction technologies (GURTs), developed to secure return on investments through protection of plant varieties, are among the most controversial and opposed genetic engineering biotechnologies as they are perceived as a tool to force farmers to depend on multinational corporations’ seed monopolies. In this work, the currently proposed strategies are described and compared with some of the principal techniques implemented for preventing transgene flow and/or seed saving, with a simultaneous analysis of the future perspectives of GURTs taking into account potential benefits, possible impacts on farmers and local plant genetic resources (PGR), hypothetical negative environmental issues and ethical concerns related to intellectual property that have led to the ban of this technology
A comprehensive study of shuttle vector & binary vector and its rules of in ...PRABAL SINGH
Vector: A vector is a DNA molecule that has the ability to replicate autonomously in an appropriate host cell and into which the DNA fragment to be cloned is integrated for cloning
MBB 501 PLANT BIOTECHNOLOGY
INFORMATION ABOUT DIFFERENT DNA MODIFYING ENZYMES
WHAT IS AN ENZYME?
Alkaline Phosphatase
Polynucleotide kinase
Terminal deoxyneucleotidyl transferase
Nucleases
Exonuclease
Bal31 Exonuclease III
Endonuclease
S1 endonulease
Deoxyribonuclease 1 (Dnase 1)
RNase A
RNase H
Restriction Endonuclease
PvuI
PvuII
Different types of endonuclease enzymes
The recognition sequences for some of the most frequently used restriction endonucleases.
Categorization of enzymes
Isoschizomers
Neoschizomers
Isocaudomers
Organogenesis, in plant tissue cultureKAUSHAL SAHU
Introduction
Definition
Types of organogenesis
Organogenesis through callus formation (indirect organogenesis)
Growth regulators for indirect organogenesis
Organogenesis through adventitious organ (direct organogenesis)
Growth regulators for direct organogenesis
Factor affecting the soot bud differentiation
Organogenic differentiation
Application of organogenesis
Conclusion
References
Nitrogen is one of the most important major limiting nutrients for most crops and other plant species. Biological Nitrogen Fixation (BNF) is an ecologically important phenomenon that can support an amount of nitrogen to compensate the difficiencies of this element. In this biologically-mediated process, a specific group of bacteria, collectivelly called rhizobia, fixed atomospheric dinitrogen (N2) via symbioses with legumes.Other free living bacteria fix nitrogen in the soil or in non specific association with plants. This biological process between rhizobium strains and their legume partners can be happened under low level of available nitrogen with help of many different genes such as nod, nif, fix, production of polysaccharides, competition, infection process, host specificity, Type I to Type VI secretion, signals of host and many other different genes that recently have been reported by scientists. The establishment of the symbiosis requires close coordination between the partners and is mediated by the exchange of diffusible signal molecules. Most recently, bacterial and plant genome-sequencing projects have added immensely to the resources available to study the symbiosis. A major event was the adoption of two genetic model legumes, Lotus japonicus and Medicago truncatula, and the genomes of both plants are currently being sequenced.Research with these model plants has now revealed the basic outlines of the plant-signaling pathways that lead to nodule formation.
1.What is plant tissue culture?
2.Production of virus free plants.
3.History.
4.Virus elimination by heat treatment.
5.Virus elimination by Meristem Tip culture.
6.Factor affecting virus eradication by Meristem Tip culture.
7.Chemotherapy.
8.Virus elimination through in vitro shoot-tip Grafting.
9.Virus Indexing.
10.Conclusion .
11.References .
The advances of modern plant technologies, especially genetically modified crops, are considered to be a substantial benefit to agriculture and society. However, so-called transgene escape remains and is of environmental and regulatory concern. Genetic use restriction technologies (GURTs), developed to secure return on investments through protection of plant varieties, are among the most controversial and opposed genetic engineering biotechnologies as they are perceived as a tool to force farmers to depend on multinational corporations’ seed monopolies. In this work, the currently proposed strategies are described and compared with some of the principal techniques implemented for preventing transgene flow and/or seed saving, with a simultaneous analysis of the future perspectives of GURTs taking into account potential benefits, possible impacts on farmers and local plant genetic resources (PGR), hypothetical negative environmental issues and ethical concerns related to intellectual property that have led to the ban of this technology
A comprehensive study of shuttle vector & binary vector and its rules of in ...PRABAL SINGH
Vector: A vector is a DNA molecule that has the ability to replicate autonomously in an appropriate host cell and into which the DNA fragment to be cloned is integrated for cloning
MBB 501 PLANT BIOTECHNOLOGY
INFORMATION ABOUT DIFFERENT DNA MODIFYING ENZYMES
WHAT IS AN ENZYME?
Alkaline Phosphatase
Polynucleotide kinase
Terminal deoxyneucleotidyl transferase
Nucleases
Exonuclease
Bal31 Exonuclease III
Endonuclease
S1 endonulease
Deoxyribonuclease 1 (Dnase 1)
RNase A
RNase H
Restriction Endonuclease
PvuI
PvuII
Different types of endonuclease enzymes
The recognition sequences for some of the most frequently used restriction endonucleases.
Categorization of enzymes
Isoschizomers
Neoschizomers
Isocaudomers
Organogenesis, in plant tissue cultureKAUSHAL SAHU
Introduction
Definition
Types of organogenesis
Organogenesis through callus formation (indirect organogenesis)
Growth regulators for indirect organogenesis
Organogenesis through adventitious organ (direct organogenesis)
Growth regulators for direct organogenesis
Factor affecting the soot bud differentiation
Organogenic differentiation
Application of organogenesis
Conclusion
References
Nitrogen is one of the most important major limiting nutrients for most crops and other plant species. Biological Nitrogen Fixation (BNF) is an ecologically important phenomenon that can support an amount of nitrogen to compensate the difficiencies of this element. In this biologically-mediated process, a specific group of bacteria, collectivelly called rhizobia, fixed atomospheric dinitrogen (N2) via symbioses with legumes.Other free living bacteria fix nitrogen in the soil or in non specific association with plants. This biological process between rhizobium strains and their legume partners can be happened under low level of available nitrogen with help of many different genes such as nod, nif, fix, production of polysaccharides, competition, infection process, host specificity, Type I to Type VI secretion, signals of host and many other different genes that recently have been reported by scientists. The establishment of the symbiosis requires close coordination between the partners and is mediated by the exchange of diffusible signal molecules. Most recently, bacterial and plant genome-sequencing projects have added immensely to the resources available to study the symbiosis. A major event was the adoption of two genetic model legumes, Lotus japonicus and Medicago truncatula, and the genomes of both plants are currently being sequenced.Research with these model plants has now revealed the basic outlines of the plant-signaling pathways that lead to nodule formation.
Presentation on genetics of nitrogen fixation by Tahura MariyamTahura Mariyam Ansari
this presentation is about what is the genetics involvement in nitrogen fixation i.e which gene is responsible etc....
the contents include Genetics of N2 fixing microorganisms, Bacterial Nodulation Genes and Regulation of nod Gene Expression, Nif Genes and their Regulation in K. Pneumoniae & Cyanobacteria, Nitrogen fixation mechanism
Nitrogenase Types, Structure and Function, Alternative nitrogenase, Substrate for Nitrogenase, Electron proteins and Hydrogen evolution
COMPUTATIONAL ANALYSIS OF CIS-REGULATORY ELEMENTS AND ASSOCIATED TRANSCRIPTIO...VartikaRai17
The plant-specific DOF transcription factors have important biological role in plant morphogenesis growth and development. In this study sequences of ten Ocimum bacilicum Dof gene promoters were analyzed. Identification of biologically significant CREs (Cis-acting regulatory elements) was performed and CREs corresponding to light response, abiotic and biotic stress response, phytohormone response, and tissue-specific elements were found. Genes promoter analysis also revealed the presence of AP2, C2H2, bZIP, bHLH, GATA, Dof, GATA, HSF, NF-Y, and Homedomain. Transcription factor binding site in the promoter region of ObDof genes. These findings not only contribute to the understanding of the gene function of candidate ObDof genes for further analysis, but the information also contributes to the understanding of the gene regulatory network.
1. NITROGEN FIXING GENES
BY
MUHAMMAD NADEEM ASHRAF
SOIL & ENVIRONMENTAL SCIENCES
nadeemawan17@gmail.com
2. NITROGEN FIXING GENES
The nif gene is the gene responsible for the coding
of proteins related and associated with the fixation of
atmospheric nitrogen into a form of nitrogen available
to plants. These genes are found in nitrogen fixing
bacteria and cyanobacteria.
3. • Specific expression of plant and bacterial genes
accompanies the development of rhizobial plant
symbiosis.
• The genes involved in the root nodule formation
collectively called Nodulin genes, encodes a series of
Nod proteins that serves specific functions in the
establishment of nodules that permit symbiotic
nitrogen fixation.
4. Classification of Genes
These genes are classified into 2 classes
The first class include the genes that specify the
biochemical composition of bacterial cell surface such
as genes determining the synthesis of
exoploysaccharides (exo genes), lipopolysaccharides
(lps genes), capsular polysaccharides of K-antigen and
β-1,2 glucans (ndv genes)
5. The exo and lps genes may play a role in determining
the host specificity but this has yet to be firmly
established.
The second class of the geses consists of the nodulation
genes (nod or nol genes).
Inactivation of the nodulation genes can result in
various plant phenotypes, such as the absence of
nodulation, or delayed but effective nodulation, or
changes in the host range.
6. Fast growing rhizobia : sym plasmid
Slow growing Brady rhizobia : bacterial chromosomes.
With in the gene cluster essential for process of
nitrogen fixation are nif and fix genes, which includes
the structural genes for the nitrogenase enzyme.
The host specific nod genes determines the specificity
of nodulation on a particular host.
7. Location of genes
Nod Genes : sym plasmid
Specificity genes : sym plasmid
Nif genes : may or may not be plasmid associated in
various rhizobial strains.
8. nif and fix genes
Beside nod genes the symplasids carry the nif and fix
clusters including the structural genes for nitrogenase.
Most rhizobium nod genes are not expressed in cultured
cells but are induced in the presence of plant.
This induction required falvonoids secreted by the plant
and also the transcriptional activator protein NodD.
The NodD gene is the only nod gene that is constitutively
expressed in both free living and symbiotic state of
Rhizobium