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Photosynthesis
2
Photosynthesis Overview
Energy for all life on Earth ultimately comes
from photosynthesis.
6CO2 + 12H2O C6H12O6 + 6H2O + 6O2
Oxygenic photosynthesis is carried out by:
cyanobacteria, 7 groups of algae,
all land plants
3
Photosynthesis Overview
Photosynthesis is divided into:
light-dependent reactions
-capture energy from sunlight
-make ATP and reduce NADP+ to NADPH
carbon fixation reactions
-use ATP and NADPH to synthesize
organic molecules from CO2
4
5
Photosynthesis Overview
Photosynthesis takes place in chloroplasts.
thylakoid membrane – internal membrane
arranged in flattened sacs
-contain chlorophyll and other pigments
grana – stacks of thylakoid membranes
stroma – semiliquid substance surrounding
thylakoid membranes
6
7
Discovery of Photosynthesis
The work of many scientists led to the
discovery of how photosynthesis works.
Jan Baptista van Helmont (1580-1644)
Joseph Priestly (1733-1804)
Jan Ingen-Housz (1730-1799)
F. F. Blackman (1866-1947)
8
Discovery of Photosynthesis
C. B. van Niel, 1930’s
-proposed a general formula:
CO2+H2A + light energy CH2O + H2O + 2A
where H2A is the electron donor
-van Niel identified water as the source of the
O2 released from photosynthesis
-Robin Hill confirmed van Niel’s proposal that
energy from the light reactions fuels carbon
fixation
9
Pigments
photon: a particle of light
-acts as a discrete bundle of energy
-energy content of a photon is inversely
proportional to the wavelength of the light
photoelectric effect: removal of an electron
from a molecule by light
-occurs when photons transfer energy to
electrons
10
11
Pigments
Pigments: molecules that absorb visible
light
Each pigment has a characteristic
absorption spectrum, the range and
efficiency of photons it is capable of
absorbing.
12
13
Pigments
chlorophyll a – primary pigment in plants
and cyanobacteria
-absorbs violet-blue and red light
chlorophyll b – secondary pigment
absorbing light wavelengths that
chlorophyll a does not absorb
14
Pigments
Structure of pigments:
porphyrin ring: complex ring structure with
alternating double and single bonds
-magnesium ion at the center of the ring
-photons excite electrons in the ring
-electrons are shuttled away from the ring
15
16
Pigments
accessory pigments: secondary pigments
absorbing light wavelengths other than
those absorbed by chlorophyll a
-increase the range of light wavelengths that
can be used in photosynthesis
-include: chlorophyll b, carotenoids,
phycobiloproteins
-carotenoids also act as antioxidants
• Carotenoids
– Carbon rings linked to
chains with alternating
single and double
bonds
– Can absorb photons
with a wide range of
energies
– Also scavenge free
radicals – antioxidant
• Protective role
• Phycobiloproteins
– Important in low-light
ocean areas
17
18
Photosystem Organization
A photosystem consists of
1. an antenna complex of hundreds of
accessory pigment molecules
2. a reaction center of one or more
chlorophyll a molecules
Energy of electrons is transferred through
the antenna complex to the reaction
center.
19
20
Photosystem Organization
At the reaction center, the energy from the
antenna complex is transferred to
chlorophyll a.
This energy causes an electron from
chlorophyll to become excited.
The excited electron is transferred from
chlorophyll a to an electron acceptor.
Water donates an electron to chlorophyll a
to replace the excited electron.
21
22
Light-Dependent Reactions
Light-dependent reactions occur in 4 stages:
1. primary photoevent – a photon of light is
captured by a pigment molecule
2. charge separation – energy is transferred
to the reaction center; an excited electron
is transferred to an acceptor molecule
3. electron transport – electrons move
through carriers to reduce NADP+
4. chemiosmosis – produces ATP
23
24
Light-Dependent Reactions
In sulfur bacteria, only one photosystem is
used for cyclic photophosphorylation
1. an electron joins a proton to produce
hydrogen
2. an electron is recycled to chlorophyll
-this process drives the chemiosmotic
synthesis of ATP
25
Light-Dependent Reactions
In chloroplasts, two linked photosystems are
used in noncyclic photophosphorylation
1. photosystem I
-reaction center pigment (P700) with a peak
absorption at 700nm
2. photosystem II
-reaction center pigment (P680) has a peak
absorption at 680nm
26
Photosystem II
• Resembles the reaction center of purple bacteria
• Core of 10 transmembrane protein subunits with
electron transfer components and two P680
chlorophyll molecules
• Reaction center differs from purple bacteria in
that it also contains four manganese atoms
– Essential for the oxidation of water
• b6-f complex
– Proton pump embedded in thylakoid membrane
27
Photosystem I
• Reaction center consists of a core
transmembrane complex consisting of 12
to 14 protein subunits with two bound P700
chlorophyll molecules
• Photosystem I accepts an electron from
plastocyanin into the “hole” created by the
exit of a light-energized electron
• Passes electrons to NADP+ to form
NADPH
28
29
Light-Dependent Reactions
Photosystem II acts first:
-accessory pigments shuttle energy to the
P680 reaction center
-excited electrons from P680 are transferred
to b6-f complex
-electron lost from P680 is replaced by an
electron released from the splitting of
water
30
Light-Dependent Reactions
The b6-f complex is a series of electron
carriers.
-electron carrier molecules are embedded in
the thylakoid membrane
-protons are pumped into the thylakoid
space to form a proton gradient
31
Light-Dependent Reactions
Photosystem I
-receives energy from an antenna complex
-energy is shuttled to P700 reaction center
-excited electron is transferred to a
membrane-bound electron carrier
-electrons are used to reduce NADP+ to
NADPH
-electrons lost from P700 are replaced from
the b6-f complex
32
Light-Dependent Reactions
ATP is produced via chemiosmosis.
- ATP synthase is embedded in the
thylakoid membrane
-protons have accumulated in the thylakoid
space
-protons move into the stroma only through
ATP synthase
-ATP is produced from ADP + Pi
33
34
35
Carbon Fixation Reactions
To build carbohydrates, cells need:
1. energy
-ATP from light-dependent reactions
2. reduction potential
-NADPH from photosystem I
36
Carbon Fixation Reactions
Calvin cycle
-biochemical pathway that allows for carbon
fixation
-occurs in the stroma
-uses ATP and NADPH as energy sources
-incorporates CO2 into organic molecules
37
Carbon Fixation Reactions
carbon fixation – the incorporation of CO2
into organic molecules
-occurs in the first step of the Calvin cycle
ribulose-bis-phosphate + CO2 2(PGA)
5 carbons 1 carbon 3 carbons
The reaction is catalyzed by rubisco.
38
39
Carbon Fixation Reactions
The Calvin cycle has 3 phases:
1. carbon fixation
RuBP + CO2 2 molecules PGA
2. reduction
PGA is reduced to G3P
3. regeneration of RuBP
G3P is used to regenerate RuBP
40
Carbon Fixation Reactions
Glucose is not a direct product of the Calvin
cycle.
-2 molecules of G3P leave the cycle
-each G3P contains 3 carbons
-2 G3P are used to produce 1 glucose in
reactions in the cytoplasm
41
Carbon Fixation Reactions
During the Calvin cycle, energy is needed.
The energy is supplied from:
- 18 ATP molecules
- 12 NADPH molecules
42
Carbon Fixation Reactions
The energy cycle:
-photosynthesis uses the products of
respiration as starting substrates
-respiration uses the products of
photosynthesis as starting substrates
43
44
45
Photorespiration
Rubisco has 2 enzymatic activities:
1. carboxylation – the addition of CO2 to
RuBP
-favored under normal conditions
2. photorespiration – the oxidation of
RuBP by the addition of O2
-favored in hot conditions
CO2 and O2 compete for the active site on
RuBP.
46
47
Photorespiration
Some plants can avoid photorespiration by
using an enzyme other than rubisco.
-PEP carboxylase
-CO2 is added to phosphoenolpyruvate
(PEP)
-a 4 carbon compound is produced
-CO2 is later released from this 4-carbon
compound and used by rubisco in the
Calvin cycle
48
Photorespiration
C4 plants
-use PEP carboxylase to capture CO2
-CO2 is added to PEP in one cell type
(mesophyll cell)
-the resulting 4-carbon compound is moved
into a bundle sheath cell where the CO2 is
released and used in the Calvin cycle
49
50
51
Photorespiration
CAM plants
-CO2 is captured at night when stomata are
open
-PEP carboxylase adds CO2 to PEP to
produce a 4 carbon compound
-this compound releases CO2 during the day
-CO2 is then used by rubisco in the Calvin
cycle
52

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GENERAL BIOLOGY - PHOTOSYNTHESIS

  • 2. 2 Photosynthesis Overview Energy for all life on Earth ultimately comes from photosynthesis. 6CO2 + 12H2O C6H12O6 + 6H2O + 6O2 Oxygenic photosynthesis is carried out by: cyanobacteria, 7 groups of algae, all land plants
  • 3. 3 Photosynthesis Overview Photosynthesis is divided into: light-dependent reactions -capture energy from sunlight -make ATP and reduce NADP+ to NADPH carbon fixation reactions -use ATP and NADPH to synthesize organic molecules from CO2
  • 4. 4
  • 5. 5 Photosynthesis Overview Photosynthesis takes place in chloroplasts. thylakoid membrane – internal membrane arranged in flattened sacs -contain chlorophyll and other pigments grana – stacks of thylakoid membranes stroma – semiliquid substance surrounding thylakoid membranes
  • 6. 6
  • 7. 7 Discovery of Photosynthesis The work of many scientists led to the discovery of how photosynthesis works. Jan Baptista van Helmont (1580-1644) Joseph Priestly (1733-1804) Jan Ingen-Housz (1730-1799) F. F. Blackman (1866-1947)
  • 8. 8 Discovery of Photosynthesis C. B. van Niel, 1930’s -proposed a general formula: CO2+H2A + light energy CH2O + H2O + 2A where H2A is the electron donor -van Niel identified water as the source of the O2 released from photosynthesis -Robin Hill confirmed van Niel’s proposal that energy from the light reactions fuels carbon fixation
  • 9. 9 Pigments photon: a particle of light -acts as a discrete bundle of energy -energy content of a photon is inversely proportional to the wavelength of the light photoelectric effect: removal of an electron from a molecule by light -occurs when photons transfer energy to electrons
  • 10. 10
  • 11. 11 Pigments Pigments: molecules that absorb visible light Each pigment has a characteristic absorption spectrum, the range and efficiency of photons it is capable of absorbing.
  • 12. 12
  • 13. 13 Pigments chlorophyll a – primary pigment in plants and cyanobacteria -absorbs violet-blue and red light chlorophyll b – secondary pigment absorbing light wavelengths that chlorophyll a does not absorb
  • 14. 14 Pigments Structure of pigments: porphyrin ring: complex ring structure with alternating double and single bonds -magnesium ion at the center of the ring -photons excite electrons in the ring -electrons are shuttled away from the ring
  • 15. 15
  • 16. 16 Pigments accessory pigments: secondary pigments absorbing light wavelengths other than those absorbed by chlorophyll a -increase the range of light wavelengths that can be used in photosynthesis -include: chlorophyll b, carotenoids, phycobiloproteins -carotenoids also act as antioxidants
  • 17. • Carotenoids – Carbon rings linked to chains with alternating single and double bonds – Can absorb photons with a wide range of energies – Also scavenge free radicals – antioxidant • Protective role • Phycobiloproteins – Important in low-light ocean areas 17
  • 18. 18 Photosystem Organization A photosystem consists of 1. an antenna complex of hundreds of accessory pigment molecules 2. a reaction center of one or more chlorophyll a molecules Energy of electrons is transferred through the antenna complex to the reaction center.
  • 19. 19
  • 20. 20 Photosystem Organization At the reaction center, the energy from the antenna complex is transferred to chlorophyll a. This energy causes an electron from chlorophyll to become excited. The excited electron is transferred from chlorophyll a to an electron acceptor. Water donates an electron to chlorophyll a to replace the excited electron.
  • 21. 21
  • 22. 22 Light-Dependent Reactions Light-dependent reactions occur in 4 stages: 1. primary photoevent – a photon of light is captured by a pigment molecule 2. charge separation – energy is transferred to the reaction center; an excited electron is transferred to an acceptor molecule 3. electron transport – electrons move through carriers to reduce NADP+ 4. chemiosmosis – produces ATP
  • 23. 23
  • 24. 24 Light-Dependent Reactions In sulfur bacteria, only one photosystem is used for cyclic photophosphorylation 1. an electron joins a proton to produce hydrogen 2. an electron is recycled to chlorophyll -this process drives the chemiosmotic synthesis of ATP
  • 25. 25 Light-Dependent Reactions In chloroplasts, two linked photosystems are used in noncyclic photophosphorylation 1. photosystem I -reaction center pigment (P700) with a peak absorption at 700nm 2. photosystem II -reaction center pigment (P680) has a peak absorption at 680nm
  • 26. 26 Photosystem II • Resembles the reaction center of purple bacteria • Core of 10 transmembrane protein subunits with electron transfer components and two P680 chlorophyll molecules • Reaction center differs from purple bacteria in that it also contains four manganese atoms – Essential for the oxidation of water • b6-f complex – Proton pump embedded in thylakoid membrane
  • 27. 27 Photosystem I • Reaction center consists of a core transmembrane complex consisting of 12 to 14 protein subunits with two bound P700 chlorophyll molecules • Photosystem I accepts an electron from plastocyanin into the “hole” created by the exit of a light-energized electron • Passes electrons to NADP+ to form NADPH
  • 28. 28
  • 29. 29 Light-Dependent Reactions Photosystem II acts first: -accessory pigments shuttle energy to the P680 reaction center -excited electrons from P680 are transferred to b6-f complex -electron lost from P680 is replaced by an electron released from the splitting of water
  • 30. 30 Light-Dependent Reactions The b6-f complex is a series of electron carriers. -electron carrier molecules are embedded in the thylakoid membrane -protons are pumped into the thylakoid space to form a proton gradient
  • 31. 31 Light-Dependent Reactions Photosystem I -receives energy from an antenna complex -energy is shuttled to P700 reaction center -excited electron is transferred to a membrane-bound electron carrier -electrons are used to reduce NADP+ to NADPH -electrons lost from P700 are replaced from the b6-f complex
  • 32. 32 Light-Dependent Reactions ATP is produced via chemiosmosis. - ATP synthase is embedded in the thylakoid membrane -protons have accumulated in the thylakoid space -protons move into the stroma only through ATP synthase -ATP is produced from ADP + Pi
  • 33. 33
  • 34. 34
  • 35. 35 Carbon Fixation Reactions To build carbohydrates, cells need: 1. energy -ATP from light-dependent reactions 2. reduction potential -NADPH from photosystem I
  • 36. 36 Carbon Fixation Reactions Calvin cycle -biochemical pathway that allows for carbon fixation -occurs in the stroma -uses ATP and NADPH as energy sources -incorporates CO2 into organic molecules
  • 37. 37 Carbon Fixation Reactions carbon fixation – the incorporation of CO2 into organic molecules -occurs in the first step of the Calvin cycle ribulose-bis-phosphate + CO2 2(PGA) 5 carbons 1 carbon 3 carbons The reaction is catalyzed by rubisco.
  • 38. 38
  • 39. 39 Carbon Fixation Reactions The Calvin cycle has 3 phases: 1. carbon fixation RuBP + CO2 2 molecules PGA 2. reduction PGA is reduced to G3P 3. regeneration of RuBP G3P is used to regenerate RuBP
  • 40. 40 Carbon Fixation Reactions Glucose is not a direct product of the Calvin cycle. -2 molecules of G3P leave the cycle -each G3P contains 3 carbons -2 G3P are used to produce 1 glucose in reactions in the cytoplasm
  • 41. 41 Carbon Fixation Reactions During the Calvin cycle, energy is needed. The energy is supplied from: - 18 ATP molecules - 12 NADPH molecules
  • 42. 42 Carbon Fixation Reactions The energy cycle: -photosynthesis uses the products of respiration as starting substrates -respiration uses the products of photosynthesis as starting substrates
  • 43. 43
  • 44. 44
  • 45. 45 Photorespiration Rubisco has 2 enzymatic activities: 1. carboxylation – the addition of CO2 to RuBP -favored under normal conditions 2. photorespiration – the oxidation of RuBP by the addition of O2 -favored in hot conditions CO2 and O2 compete for the active site on RuBP.
  • 46. 46
  • 47. 47 Photorespiration Some plants can avoid photorespiration by using an enzyme other than rubisco. -PEP carboxylase -CO2 is added to phosphoenolpyruvate (PEP) -a 4 carbon compound is produced -CO2 is later released from this 4-carbon compound and used by rubisco in the Calvin cycle
  • 48. 48 Photorespiration C4 plants -use PEP carboxylase to capture CO2 -CO2 is added to PEP in one cell type (mesophyll cell) -the resulting 4-carbon compound is moved into a bundle sheath cell where the CO2 is released and used in the Calvin cycle
  • 49. 49
  • 50. 50
  • 51. 51 Photorespiration CAM plants -CO2 is captured at night when stomata are open -PEP carboxylase adds CO2 to PEP to produce a 4 carbon compound -this compound releases CO2 during the day -CO2 is then used by rubisco in the Calvin cycle
  • 52. 52