Surviving animals produced by nuclear transfer are healthy. There, is a substantial loss of individual before and after birth some of the cloned animals display abnormalities. Abnormlities such as increased birth weight. Dna methylation and histone modification of the original donor cell is inappropriate maintained in the cells of the recipient animals. INVIVO: The direct delivery of the therapeutic gene into the target cells of a particular tissue. Genes are changed in cells when the cells are still in the body. These include liver, muscle, skin, spleen, lung, brain and blood cells. Carried out by viral and non viral vector system EXVIVO: Cells are modified outside the body and then transplanted back in again. SCID is a inherited immune disorder associated with T-lymphocyte and B-lymphocytes dysfunction. Gene encodes for adenosine deaminase. The patients of SCID(lacking ADA) suffer from infectious diseases and die at an young age.

1. GENE THERAPY
BY,
SATHYA THIRUPATHI,
MSC MICROBIOLOGY,
TIRUPUR KUMARAN COLLEGE FOR WOMEN,
TIRUPUR

2. INTRODUCTION:
• Gene therapy is the process of
inserting genes into cells to treat
diseases
• Gene therapy involves genetic
manipulations in animals or humans
to correct disease and keep them
healthy.

3. TYPESSomatic
Gene
Therapy
Germ-line
Gene
Therapy

4. INVIVO GENE THERAPY:
The direct delivery of the
therapeutic gene into the target
cells of a particular tissue.
Genes are changed in cells when
the cells are still in the body.
These include liver, muscle, skin,
spleen, lung, brain and blood cells.
Carried out by viral and non viral
vector system

5. • Parametres:
I. The efficiency of the uptake of remedial gene by
the target cells.
II. Intracellular degradation of the gene and its uptake
by nucleus.
III. The expression capability of the gene.

7. EXVIVO GENE THERAPY:
Cells are modified outside the body
and then transplanted back in again.
SCID is a inherited immune disorder
associated with T-lymphocyte and B-
lymphocytes dysfunction.
Gene encodes for adenosine
deaminase.
The patients of SCID(lacking ADA)
suffer from infectious diseases and die
at an young age.