Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
Today’s analytical laboratory is faced with tight deadlines to produce results from testing environmental samples. Too often, solid-phase extraction (SPE) presents a bottleneck in the analytical testing process and may cause poor analyte recoveries and highly variable. Despite advances in analytical instrumentation, sample prep often relies on tedious, manual, and expensive techniques such as liquid-liquid extraction.
Sample preparation of environmental water samples can be automated, however.. Use of automated sample preparation addresses the many challenges that laboratories face when preparing samples and can help improve sample processing turnaround times.
Chromatography presentation goes with this free on-demand webinar. Link to webinar: https://event.on24.com/eventRegistration/EventLobbyServlet?target=registration.jsp&eventid=832348&sessionid=1&key=7401504685427A0804ABBD1F956E617C&partnerrefthermo=undefined&sourcepage=register
Fat determination by Büchi. Comparison between soxhlet and hot extractionLoïc Ehanno
Fat determination is one of the key analyses used for food labelling and quality control. Two extraction methods were compared with respect to practicality and reliability of the results.
Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
Today’s analytical laboratory is faced with tight deadlines to produce results from testing environmental samples. Too often, solid-phase extraction (SPE) presents a bottleneck in the analytical testing process and may cause poor analyte recoveries and highly variable. Despite advances in analytical instrumentation, sample prep often relies on tedious, manual, and expensive techniques such as liquid-liquid extraction.
Sample preparation of environmental water samples can be automated, however.. Use of automated sample preparation addresses the many challenges that laboratories face when preparing samples and can help improve sample processing turnaround times.
Chromatography presentation goes with this free on-demand webinar. Link to webinar: https://event.on24.com/eventRegistration/EventLobbyServlet?target=registration.jsp&eventid=832348&sessionid=1&key=7401504685427A0804ABBD1F956E617C&partnerrefthermo=undefined&sourcepage=register
Fat determination by Büchi. Comparison between soxhlet and hot extractionLoïc Ehanno
Fat determination is one of the key analyses used for food labelling and quality control. Two extraction methods were compared with respect to practicality and reliability of the results.
Equilibrium data and related information gathered from a liquid-liquid extraction laboratory “shake test” can provide information for process feasibility and column-type selection in the scaleup of liquid-liquid extraction processes
Most chemical engineers have had the experience of dealing with problematic separations, and most have a general understanding of distillation processes. When it comes to liquid-liquid extraction (LLE) processes (Figure 1), however, the details of how these processes work are often less clear. Most academic chemical engineering degree programs do not heavily emphasize liquid-liquid extraction, and most chemical engineering graduates did not receive more than a few days of instruction on generating equilibrium data for LLE in their degree programs.
A Soxhlet extractor is a piece of laboratory apparatus invented in 1879 by Franz von Soxhlet. It was originally designed for the extraction of a lipid from a solid material. However, a Soxhlet extractor is not limited to the extraction of lipids. Typically, a Soxhlet extraction is only required where the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent. If the desired compound has a high solubility in a solvent then a simple filtration can be used to separate the compound from the insoluble substance.
The presentation is based on Mass Transfer the major subject of Chemical Engineering. It includes
Separation
Technologies
Typical Applications
Industries
Distillation vs. Extraction
LLE Extraction
LLE extraction Principle
Solvent
Operation Condition
Mode of Operation
Extractor Type
Design Criteria
Equipment for extraction
The use of a machine designed to follow repeatedly and automatically a predetermined sequence of individual operations.
AUTOMATED WASHING
AUTOMATED MEDIA PREPARATORS
AUTOMATED COLLECTION AND
PROCESSING OF SAMPLES
CYTOSPIN
AUTOMATED GRAM STAINING
AUTOMATED STREAKING
SPIRAL PLATER
AUTOMATED ANTIBIOTIC -
SENSITIVITY SYSTEM
AUTOMATIC COLONY COUNTER
AUTOMATED URINE MICROSCOPY -
ANALYSER
Equilibrium data and related information gathered from a liquid-liquid extraction laboratory “shake test” can provide information for process feasibility and column-type selection in the scaleup of liquid-liquid extraction processes
Most chemical engineers have had the experience of dealing with problematic separations, and most have a general understanding of distillation processes. When it comes to liquid-liquid extraction (LLE) processes (Figure 1), however, the details of how these processes work are often less clear. Most academic chemical engineering degree programs do not heavily emphasize liquid-liquid extraction, and most chemical engineering graduates did not receive more than a few days of instruction on generating equilibrium data for LLE in their degree programs.
A Soxhlet extractor is a piece of laboratory apparatus invented in 1879 by Franz von Soxhlet. It was originally designed for the extraction of a lipid from a solid material. However, a Soxhlet extractor is not limited to the extraction of lipids. Typically, a Soxhlet extraction is only required where the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent. If the desired compound has a high solubility in a solvent then a simple filtration can be used to separate the compound from the insoluble substance.
The presentation is based on Mass Transfer the major subject of Chemical Engineering. It includes
Separation
Technologies
Typical Applications
Industries
Distillation vs. Extraction
LLE Extraction
LLE extraction Principle
Solvent
Operation Condition
Mode of Operation
Extractor Type
Design Criteria
Equipment for extraction
The use of a machine designed to follow repeatedly and automatically a predetermined sequence of individual operations.
AUTOMATED WASHING
AUTOMATED MEDIA PREPARATORS
AUTOMATED COLLECTION AND
PROCESSING OF SAMPLES
CYTOSPIN
AUTOMATED GRAM STAINING
AUTOMATED STREAKING
SPIRAL PLATER
AUTOMATED ANTIBIOTIC -
SENSITIVITY SYSTEM
AUTOMATIC COLONY COUNTER
AUTOMATED URINE MICROSCOPY -
ANALYSER
INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip.
This technology enables INTip solid phase extraction for easy sample preparation. The disperser helps to perturb the sample solution and loose sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries.
Basic Approach to Dissolution Method Development – Challenges and Regulatory ...Dr. Harshal Pawar
This presentation explains the theoretical as well as practical aspects of dissolution. It provides a systematic and scientific path for development of dissolution method for a new pharmaceutical product.
Kuecept Ltd was founded in 2007 by a group of experienced industrial scientists to provide customised R&D solutions and consultancy services to the pharmaceutical, biotech and health-care industries.
Today, we are one of a few contract research organisations dedicated solely to providing preformulation, formulation development and enabling drug delivery services to companies in the discovery / preclinical stages. By working exclusively in this field, we have developed a wealth of knowledge and expertise of enabling drug delivery technologies and formulation know-how in drug solubility and bioavailability enhancement and with over 600 projects completed to date on over 250 NCEs, are well placed to help resolve some of the most complex drug development issues.
Our experience covers a broad range of discovery, development & related activities supporting oral, parenteral and orally / nasally inhaled drug products.
Single-Use Tangential Flow Filtration for Closed ProcessingMerck Life Sciences
Watch the presentation of this webinar here: https://bit.ly/3b7vD60
Closed processing involves use of physical barriers to separate processing fluid from the external environment. This approach reduces capital expenditures and clean room classification while accelerating time to market. This webinar will present a TFF process run in a closed mode.
Closed processing with single-use technologies is a critical enabler for efficient and robust manufacturing for novel modalities as well as continuous biomanufacturing processing. It can also reduce the dependence on classified clean rooms for traditional modalities. This approach helps to mitigate the risk of contamination by adventitious agents while enhancing operator safety.
In this presentation, we discuss the implementation of closed processing for downstream applications and present the design and performance testing of a single use manufacturing-scale tangential flow filtration system to be able to operate in both functionally and fully closed mode.
In this webinar, you will learn:
• The context of closed processing
• Differences between closed and functionally closed processing
• The drivers for adoption
• Its practical implementation to a TFF step
Single-Use Tangential Flow Filtration for Closed ProcessingMilliporeSigma
Watch the presentation of this webinar here: https://bit.ly/3b7vD60
Closed processing involves use of physical barriers to separate processing fluid from the external environment. This approach reduces capital expenditures and clean room classification while accelerating time to market. This webinar will present a TFF process run in a closed mode.
Closed processing with single-use technologies is a critical enabler for efficient and robust manufacturing for novel modalities as well as continuous biomanufacturing processing. It can also reduce the dependence on classified clean rooms for traditional modalities. This approach helps to mitigate the risk of contamination by adventitious agents while enhancing operator safety.
In this presentation, we discuss the implementation of closed processing for downstream applications and present the design and performance testing of a single use manufacturing-scale tangential flow filtration system to be able to operate in both functionally and fully closed mode.
In this webinar, you will learn:
• The context of closed processing
• Differences between closed and functionally closed processing
• The drivers for adoption
• Its practical implementation to a TFF step
Kjeltec ™ 8400 là máy phân tích Kjeldahl hoàn toàn tự động với máy đo màu tích hợp chuẩn độ và bộ lấy mẫu tự động tùy chọn hoàn hảo cho phòng thí nghiệm với lượng mẫu lớn.
NIRS ™ DS2500 cung cấp phân tích NIR nhanh chóng với độ chính xác đặc biệt nhiều loại mẫu. Kết nối với FossManager ™ mang lại lợi ích đã được chứng minh
Acusera Verify Linearity Verification - June 17 LT674Randox
Our Acusera Linearity Sets are perfect for laboratories wishing to challenge the full reportable range of their instruments. With sets available for Roche & Beckman analysers, our sets are sure to meet all your laboratory and regulatory requirements.
Pilot plant Techniques and Product consideration for liquid dosage forms.D.R. Chandravanshi
CONTENTS:-
DEFINITION
INTRODUCTION
OBJECTIVES
LIQUID DOSAGE FORM
STEPS INVOLVED IN PILOT PLANT FOR ORAL LIQUID
GENERAL CONSIDERATION
Reporting responsibility
Personal requirements
Space requirements
Review of formula
Raw materials
Relevant processing equipments
Process evaluation
GMP consideration
Assurance
PILOT PLANT SCALE UP FOR SUSPENSION
PILOT PLANT SCALE UP FOR EMULSION
REFERENCES
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Maxilla, Mandible & Hyoid Bone & Clinical Correlations by Dr. RIG.pptx
Zinsser Analytic Info & News 2018
1. Info & News
2018
CONTENT
Extraction of Micro-Organisms 1
Milk Sample Preparation 2
Viscous Liquid Blending 2
Powder Dispensing 3
Polyvials® DS 3
Automated SPE and Filtration 4
Measurements on LOGP 4
Liquid-Liquid Extraction 5
Pre-Formulation Studies 5
SOPHAS – A Genius Synthesis
Concept
6
Soil Contamination Analysis 6
Solutions for Substance
Libraries
7
Pipetting through Thick Seals 7
Minilab® 8
Liquid Scintillation Counter
LSC-8000
8
Polyvials® & Qualydrops® 9
Scintillation Cocktails 10
Low-Level Tritium Counting 10
EXTRACTION OF MICRO-ORGANISMS
Freeze-dried micro-organisms are
presented to the system in 24-position
deep-well plates and 4 ml of methanol
are pipetted to each well. To optimise
the extraction process, the sample
material is disrupted and mixed
with an Ultra-Turrax® mixer for three
seconds. The plate is then tilted and
the total volume of sample “slurry”
present is transferred with special
probes to 24-position filter plates.
Filtration occurs under vacuum or
positive pressure into a 24-position
deep-well receiving plate positioned
below. The workbench is configured
with stacker assemblies for 21 sample
plates, 21 filter plates and 21 receiving
plates therefore 21 sample plates (504
extractions in total) can be processed
in one run without user intervention.
2. LISSY® – MILK SAMPLE PREPARATION
LISSY® is a versatile system as typified by the wide range
of applications it can process. A further example is the pre-
paration of milk samples for QC testing, which involves a
variety of different experimental methods such as inhibitor
tests, or the detection of pathogens like feline leukaemia
and brucellosis. Boxes of raw milk samples from the dairies
containing samples in cartons or bottles (80 – 100 per box)
are delivered directly to the analysis laboratory for the
detection of residues or pathogens by ELISA. Through-
put demand can be as high as 1,000 samples per day from
each dairy. The milk samples are aspirated directly from
the sample bottles or milk cartons and pipetted into a
microtitre plate, including standards and controls. After
reagent addition and incubation, the microtitre plates are
read using a photometer. All samples are barcoded and
the work list for the run, either as an Excel® or a CSV file is
uploaded from the network at point of run. Each dairy can
supply a varying number of samples for analysis on a day-
to-day basis and priorities for preparation within the batch
can also vary. The software of LISSY® ensures that samples
are pipetted consecutively, that batches can be added on
the fly based on priority and that the start position within
a batch can be also determined based on priority with
transfer occurring to the corresponding position in the
microtitre plate. Our in-house team of designers, engineers
and software specialists enabled this complete solution for
the QC testing of milk samples.
VISCOUS LIQUID BLENDING & LUBRICANT DEVELOPMENT
BLENDA systems are used in industry to
speed up the development of new lubricants
for any application. Blenda 1 was designed for
medium size blends of oils and additives up to
500 ml, Blenda 2 for complex formulations
with inline viscosity adjustment. Both systems
can produce up to 40 blends per day.
BLENDA has been designed for the preparation
of complex blends of lubricants, oils and
other media (solids, liquids or compounds of
high viscosity).
Special pumps, liquid dispensing tools on a
2.5 m liquid handling workbench with two
independently operating arms and powerful
software allow high throughput blending
without compromise in precision or accuracy.
2
3. POWDER HANDLING WITH REDI-VARIX®
Our REDI® powder handling system can be used for any
workflow requiring powder handling in the range of 0.5 mg
to 2 g, such as the distribution of small quantities of solid
samples from compound libraries for screening tests, the
distribution of samples of freeze-dried plant material for
extraction or just the general pipetting of powders of all
types. The powder is aspirated from the source container
using a special powder handling probe and tip and is
dispensed gravimetrically to a set weight on an iterative
balance.
In addition, the initial source container can also be weighed
prior to and post aspiration as a pipetting check and the
destination vessel can also be weighed pre and post
dispensing. Depending on the powder properties, a
precision of ≤ 10% can be achieved, even at a low
dispense level of a 1 mg dispense with a throughput of
20 – 60 samples per hour. REDI-Varix® can be supplied in
conjunction with other system tools on a robotic platform –
as pure solid dosing only with no liquid handling or as an
integrated tool with liquid handling for sample preparation
or synthesis workflows. The integrity of the powder
samples is maintained with the use of inert gas shielding
for hygroscopic powders and efficient deionisation
modules for statically charged samples.
POLYVIALS® DS WITH TAMPER-EVIDENT CAPS
Our Polyvials® D are now also available
with tamper-evident caps!
They are manufactured under dust-
free conditions from a special poly-
ethylene for pharma packaging.
A coloured ring secures the screw cap.
When unscrewing the cap for the first
time, the colour ring will separate from
the cap. When the cap is unscrewed,
the ring will stay on the neck and can
be used for identification of the ingre-
dient. The caps are self-sealing and
are certified for airline transportation.
Rings and caps are available in multi-
ple colors. Volumes available are 15,
30, 60, 125, 250, 500 and 1000 ml.
3
4. AUTOMATED SPE AND FILTRATION SAMPLE PREPARATION FOR
PESTICIDE AND RESIDUE ANALYSIS
Speedy® works with nearly any kind of SPE cartridges, filt-
ration plates and extraction plates. With four independently
working pipetting channels (needle, disposable tip, piercing
needle), samples are transferred very fast from the sample
tubes to the extraction cartridges. With the integrated six-
way valve, Speedy® can equilibrate and wash the extraction
matrix with high volume of up to six different solvents very
quickly. The buffers are stored below or beside the working
area to save valuable place.
Speedy® eluates the samples with vacuum or with adjust-
able positive pressure and pressure control to prevent fatal
drying of sensitive matrices like affinity columns which are
used in the analytics of mycotoxines, steroides or anti-
biotics in food, blood and urine.
Speedy® can also handle higher sample volumes, which are
common in food and pesticide analysis like the QuEChERS
method. The integrated robotic arm can transport racks,
vials, extraction plates, filtration plates, microtitre plates or
any kind of tools.
Speedy is controlled via the flexible and easy-touse
software WinLISSY®. WinLISSY® gives you access to all
method steps and to all liquid handling parameters, which
is a very powerful tool for the development, adaption and
optimization of methods.
MEASUREMENTS ON LOGP, LOGD AND KINETIC SOLUBILITY
ACCELERATES EARLY DRUG DISCOVERY RESEARCH
Current medicinal chemistry is under pressure to accelerate
the outcome of research results. Early determination of as
much data as possible from new potential drugs is crucial
to fulfill this difficult target. A broad knowledge of the
properties of all compounds involved in a research project
helps the scientists to make the right decision which lead
structures they should follow. Zinsser Analytic has
developed a sample preparation system that can prepare a
complete set of HPLC samples for an exact measurement
for kinetic solubility, logP and logD just using a tiny amount
of 0.3 μmol. The system, which was developed for a major
pharmaceutical company, just needs 30 μl of 10 mM DMSO
solution per compound provided in Remp® tubes in a 384-
well format. High precision liquid handling allows the fully
automated preparation of two HPLC samples for all three
measurements (double determination). In the case of logD,
the partition coefficient is determined from the equilibra-
tion between buffer (pH 7.4) and n-octanol by separating
the two phases in a centrifuge and taking aliquots from
both phases. HPLC samples ready for direct analysis can be
prepared for 96 different compounds in 16 h. The high
throughput makes it possible to automatically measure
logP, logD and kinetic solubility for all compounds involved
in a project and to screen entire compound collections.
4
5. LIQUID-LIQUID EXTRACTION OF SYNTHETIC SUBSTANCES
Our LISSY® system allows the full
automation of the isolation of a sub-
stance of interest through liquid liquid
extraction. In this case, the substance
to be isolated is present on the walls of
30 ml sample tubes that have previous-
ly been evaporated to dryness. These
tubes are accommodated on the work-
bench in racks with an 8x12 format. To
solubilise the dried components in an
organic phase, a limited volume only
(approx. 1 ml) can be used. The organic
phase is therefore added to these tubes
using a pipetting probe that sprays
the walls of the tube with the solvent
as it is dispensed. The pipetting probe
also adds the aqueous phase via a
second channel and, depending on the
workflow, further organic solvent can
also be added with the use of a six-way
valve assembly. The total amount of
aqueous and organic phase is 10 ml.
Full mixing of the phases and hence ex-
traction of the component of interest is
achieved using an adjustable eight-fold
overhead stirrer (Ultra-Turrax® stirrer
with eight impellers) with the extraction
step and the formation of the interface
between the two phases taking only a
few seconds. The final step is the trans-
fer of the phase of choice (organic) to
6 ml filter cartridges for sample filtra-
tion under positive pressure from
above with the substance of interest
collected in receiving vials below.
CRISSY® AND SUSY FOR PRE-FORMULATION STUDIES
Good solubility and appropriate logD
values are not the only important prop-
erties of potential drug compounds.
Crystal structures and polymorphism of
these compounds alone or in combina-
tion with the excipients in pharmaceuti-
cal formulations have a huge impact on
their stability and durability. Together
with our worldwide customers in
pharmaceutical R&D and institutes we
have developed a variety of bench-top
automation systems for the preparation
of formulations and subsequent crystal-
lization experiments. These automated
workflows allow a systematic formula-
tion screening and polymorphism
studies under reproducible conditions.
Based on the requirements of our
customers, our experience and the use
of proven tools from our toolbox, we
design and build an optimized system
to fulfill your needs from the first day in
use.
CRISSY® for polymorph screening using
special reactor blocks for direct analysis
of the crystals in an XRPD X-ray spectro-
meter.
SuSy for solubility testing with specially
developed stirrer units, pH monitoring
and much more.
Formula X® for formulation studies, with
the capability to optimize existing for-
mulations or to create new ones.
5
6. SOPHAS® – A GENIUS SYNTHESIS CONCEPT FOR CATALYSTS
In today’s rapidly changing market
place, catalyst researchers are forced
to produce results to an ever-higher
standard within a limited time scale.
The key to achieving this goal is to
utilise high-throughput techniques
to efficiently generate the necessary
experimental data quickly, thus con-
trolling expenditure. Zinsser Analytic
offers a wide range of tools for the
high throughput development of cata-
lysts. These range from manual parallel
systems through to a real robotic
“research catalyst factory”. Available
features include the complete work-
flow of precursor preparation, synthe-
sis, impregnation, co-precipitation,
calcinations, sieving, tablet forming
etc. Smaller, less expensive systems
are available as dedicated work-
stations for a portion of the workflow.
For parallel scale up in the range of
100 - 300 ml systems have been
engineered. Maximum flexibility is
achieved on all our systems, which are
controlled by our proprietary Zinsser
Analytic software. Methods can be
simply generated and communication
with existing databases and informa-
tion systems is made easy.
Sophas with Reflux reactors Sophas CAT HT for high throughput synthesis
Desyre-reactor block
SAMPLE PREPARATION FOR SOIL CONTAMINATION ANALYSIS
Sample preparation for environmental
monitoring of soils (MOSH, PAH, PAK,
PCB, AOX, EOX) results in many tests
per original sample and it is time-
consuming, tedious manual work and
the technicians are exposed to hazard-
ous solvent vapors like n-hexane or
cyclohexane. The samples have to be
prepared and analyzed reliably and
without delay. MOPS works up more
than 70 soil samples per day for the
automatic analysis of contamination
by mineral oils, hydrocarbons and
PAK/PCB/EOX. The system is loaded
with the soil samples and, after the
fully automatic preparation and ex-
traction procedure, produces ready
to use crimped sample GC/LC vials.
The methods are according to the
legal regulations of the original
manual procedures but without the
human errors. The procedures include
precise liquid handling for ISTD addi-
tion, shaking and ultrasonic agitation,
centrifugation, SPE, evaporation, vial
capping/crimping and more. For a
higher throughput, the system is
equipped with two independently
working robotic arms on one platform.
Both arms are assigned to certain
tasks but they can also work together,
e.g. one arm moves the vials to the
capping station while the other adds
internal standard. MOPS will be the
new standard in environmental
analysis laboratories.
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7. SOLUTIONS FOR SUBSTANCE LIBRARIES
Huge numbers of valuable compounds are taken from
compound collections every day to prepare solutions which
are transferred to test plates and vials for biological assays.
Zinsser Analytic has delivered many systems to almost all
major pharmaceutical companies and institutes, which en-
sure fast, efficient and compound saving handling.
proForma® systems automate tare weighing, weighing,
capping, barcode reading, sorting and formatting of
sample vials. Samples are ready to store in compound
collections and proForma’s software can be integrated
into any compound logistic database. Smaller systems can
handle hundreds of bottles while production type systems
for a throughput of thousands of bottles per day are
usually equipped with multiple scales, cap feeders and
parallel capping stations.
Librarian a newly developed powder handling system
which can withdraw very small volumes (0,5 mg - 50 mg)
of sample material from storage vials for screening tasks –
even from the corners of the vial bottoms – with highest
precision, and automatically weigh in into the dissolution
vials.
MOSS systems are specialized in the preparation of
compound solutions with exact concentrations. In most
cases compounds are dissolved in DMSO which is being
accelerated by stirring, shaking and ultrasonic agitation.
Inert atmosphere (Ar/N2) can be provided. Optionally,
a camera and image interpretation software can be
implemented to insure complete dissolution and to take
logical decisions for an automated subsequent processing
(i.e. prolonged agitating or adding more solvent).
PIPETTING THROUGH THICK SEALS ...
Is it possible to access containers which are sealed with
thick rubber stoppers with a pipetting needle? Yes! We
have solved this challenge by redesigning our robot to use
commercially available injection needles instead of normal
disposable tips for pipetting. Using this new design along
with our clever software, we have produced systems for
the quality control of injection medium, where samples are
removed from the finished, stoppered injection vials and
cartridges prior to QC analysis and radioactive tracer is
then subsequently added to closed vials. The new design
ensures contamination free sampling and precise and
reproducible pipetting.
7
8. MINILAB®
For more than 20 years, the Minilab® – our semi-micro
glassware kit – has been used with great success by many
schools and research centres to conduct chemistry experi-
ments and specialized chemistry training. A new textbook
by Prof. Michael Schallies with information ranging from
basic chemistry functions to organic syntheses is now
available. The detailed experiment instructions contain
the corresponding references and safety regulations. The
Minilab® is characterized by its unique design: All parts are
easily combinable with the PTFE threaded couplings and
can be attached to a stainless steel plate using magnetic
clamps or clipped onto a tripod.
8
LIQUID SCINTILLATION COUNTER ACCUFLEX LSC-8000
AccuFLEX LSC-8000 of Hitachi
ensures accuracy and flexibility for
any basic liquid scintillation measure-
ment in research and environmental
laboratories. Quantification of all the
beta emitters (with optional alpha/
beta separation) is performed using
4000 channels and optimized energy
ranges. Pre-set counting windows or
Auto Isotope Selection offers any re-
sult units in counts, cpm, dpm or Bq.
LSC-8000 can be used with either
20 ml, 8 ml, 6 ml glass or plastic vials
or micro tubes, with a 408 samples
loading capacity in its racks-based
samples changer.
Accurate measurements are provided
with External Standard Channel Ratio,
Self-Constant Channel Ratio methods
and other advanced features such
as Chemical Luminescence, Color
Quenching Corrections and Anti-Static
Function.
Data reliability is fully supported by
a System Performance Evaluation,
including History Review and Security
locks, in a GLP mode, with 21 CFR Part
11 included as a standard feature.
Windows-based user friendly software
and built-in PC with touch panel color
LCD and various output interfaces
gives all the comfort required on a
modern, easy-to-use standard liquid
scintillation counter
9. POLYVIALS® & QUALYDROPS®
9
POLYVIALS® & QUALYDROPS®
Our Polyvials® V range from 4 to 250 ml with self-sealing,
leak-proof screw caps and are widely used for packaging
valuable reagents in the diagnostic industry. They are man-
ufactured from selected polyethylene and polypropylene in
cleanroom facilities (Class 10.000, ISO 7, USTD 209E). On-
line quality control assures consistent high quality.
Polyvials® V
These vials have set a standard in diagnostic packaging.
Their special design allows easy filling and retrieval of re-
agents. The caps allow gastight sealing even under extreme
temperatures and pressure. This makes them safe for air
transportation. The vials are supplied in natural (opaque) or
in lightproof black polyethylene. The caps are made from
polypropylene and are available in various colors.
Polyvials® D & DS
They are thick-wall packaging vials with wide caps for dry
and liquid reagents and media. They are supplied in sizes
ranging from 15 ml to 1.000 ml volume in natural (opaque)
virgin polyethylene. They are suitable for gamma or elec-
tron beam sterilization.
Polyvials® DS
These are Polyvials® D with special tamper-evident caps.
After unscrewing the vial, a color ring stays on the neck of
the vial for identification. Also, coloured caps are available
on request.
Micro-Polyvials® 1.5 ml
They are ideal for shipping and storing small quantities.
The conical bottom allows complete retrieval of the con-
tent. The unique screw caps seal without any additionally
inserted septum or “O”-ring.
Qualydrops® Dropper Bottles
They have a special dropper insert which delivers preci-
se volumes, i.e. for water the drop size is 40 μl ± 5%. The
bottles are precision molded from LDPE. They are available
in the standard sizes of 3 ml, 5 ml, 10 ml, 20 ml and 30 ml
bottles in white. Natural and black vials can be manufac-
tured on request.
9
10. ULTRA-LOW-LEVEL TRITIUM COUNTING
The new LB-7 of Hitachi LSC-LB is the only real low-level
scintillation counter. The detection limit is better than 0,1 Bq
per liter of water. The counter uses 145 ml vials which allow
to count seven times the volume per sample compared to
other low volume systems. Tritium counting efficiency with
145 ml vials is better than 25% for 70 ml of sample and
70 ml of our special low level cocktail „Quicksafe 400“.
1010
SCINTILLATION COCKTAILS
For 42 years Zinsser Analytic has been
supplying scintillators and consum-
ables for liquid scintillation counting.
Zinsser has had a great influence on
the development of scintillation cock-
tails and sample vials, as with our first
cocktail Unisolve 1 and our well proven
Polyvials which have a unique profile
for increased stability and a superior
cap sealing system which guarantees
a gas tight sealing, even at fluctuating
pressure or temperature and which
are used by researchers around the
world. Since May 2012, we have been
offering Supersolve-X, a newly
developed safe scintillator which
stands for high sample capacity –
even for alkaline solutions – and
outstanding efficiency. Contact us for
a data sheet and sample for testing!