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At the end of the lesson, students will be able to:
1. Discuss how fungi cause diseases
2. Compare and contrast superficial, cutaneous,
subcutaneous, systemic and opportunistic
mycoses in terms of characteristics/definition,
example of diseases it may cause and its
causative agent and laboratory diagnosis
 Growth on body surfaces.
 Invasion of the body.
 Allergic reactions.
 Toxins released after ingestion
Mycoses: fungal diseases.
Tend to be chronic because fungi grow slowly.
Fungal diseases are classified into 4 groups:
•Superficial mycoses
•Cutaneous mycoses
•Subcutaneous mycoses
•Deep (systemic) mycoses
Superficial Cutaneous
Definition Infection that do not involve
a tissue response
Infections of the skin, hair &
nails
Example &
Causative
Agent
Pityriasis: dermatitis
characterized by redness of
the skin and itching caused
by Malassezia furfur
Onychomycoses (Tinea
ungium) : nail infections
caused by Candida albicans
or a dermatophytes
Ringworm(Tinea capitis)
Athlete’s foot(Tinea pedis)
Jock itch(Tinea cruris)
Favus: destruction of the hair
follicle caused by
Trichophyton schonleini
Lab Diagnosis Direct Microscopy: 10% KOH
is used to digest the skin
debris(bottle shaped
budding yeast cells)
PCR & other molecular
methods
Direct Microscopy: septate
branched hyphae
PCR & other molecular
methods
Culture SDA(Sabourauds dextrose agar
medium is used)
Creamy colonies appear
SDA
Honey-combed like
thallus(cream colored to
Subcutaneous Systemic
Definition Infections beneath the skin Infections deep within the
body
Example &
Causative
Agent
Sporotrichosis: chronic
infection of the
subcutaneous tissues and
adjacent lymphatics
characterized by nodular
lesions(Sporothrix schenkii)
Histoplasmosis (Histoplasma
capsulatum): Initial infection
in lungs. Later spreads
through blood to most
organs.
Coccidiomycosis (Coccidioides
immites): a dimorphic fungus.
Resembles tuberculosis
Lab Diagnosis Direct Microscopy: Tissue
sections should be stained using
Grocott's methenamine
silver (GMS)
Gram stain
Interpretation: Look for small
narrow base budding yeast cells
(2-5um).
Clinical material- Skin scrapings,
sputum and bronchial washings,
cerebrospinal fluid, pleural fluid
and blood, bone marrow, urine
and tissue biopsies from various
visceral organs.
2. Direct Microscopy:
Skin scrapings should be
examined using 10% KOH and
Parker ink or calcofluor white
mounts.
Exudates and body fluids should
be centrifuged and the sediment
examined using either 10% KOH
FUNGAL DISEASES
Opportunistic mycoses: Caused by organisms that
are generally harmless unless individual has
weakened defenses:
◦ AIDS and cancer patients
◦ Individuals treated with broad spectrum antibiotics
◦ Very old or very young individuals (newborns).
 Examples:
◦ Aspergillosis: Inhalation of Aspergillus spores.
◦ Yeast Infections or Candidiasis: Caused mainly by Candida
albicans. Part of normal mouth, esophagus, and vaginal flora.
 Candidia albicans
◦ a common unicelluar fungus which is part of the
flora of the oral cavity, vagina, and
gastrointestinal tract;
◦ They become opportunist especially in
immunocompromised people
 Oral candidiasis (Oral thrush)
 Classic thrush is characterised by a white, curd-like
coating on the tongue or elsewhere in the oral
cavity. Stomatitis due to Candida is often
associated with painful infection of the lips - and
corners of the mouth.
 Genital or perigenital candidiasis ((vulvo vaginitis)
 (vulvo)vaginitis due to Candida .
 Contamination of the vagina with Candida stems from
the endogenous endosaprophytic flora of the gastro-
intestinal tract.
 Diagnoses
◦ in culture, it grows as blastospores, pseudohyphae,
and septate hyphae
◦ candidiasis the collective term for infection
involving Candida
 cutaneous
 vaginal
 systemic

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Fungal diseases (Mycoses).ppt

  • 1.
  • 2. At the end of the lesson, students will be able to: 1. Discuss how fungi cause diseases 2. Compare and contrast superficial, cutaneous, subcutaneous, systemic and opportunistic mycoses in terms of characteristics/definition, example of diseases it may cause and its causative agent and laboratory diagnosis
  • 3.  Growth on body surfaces.  Invasion of the body.  Allergic reactions.  Toxins released after ingestion
  • 4. Mycoses: fungal diseases. Tend to be chronic because fungi grow slowly. Fungal diseases are classified into 4 groups: •Superficial mycoses •Cutaneous mycoses •Subcutaneous mycoses •Deep (systemic) mycoses
  • 5. Superficial Cutaneous Definition Infection that do not involve a tissue response Infections of the skin, hair & nails Example & Causative Agent Pityriasis: dermatitis characterized by redness of the skin and itching caused by Malassezia furfur Onychomycoses (Tinea ungium) : nail infections caused by Candida albicans or a dermatophytes Ringworm(Tinea capitis) Athlete’s foot(Tinea pedis) Jock itch(Tinea cruris) Favus: destruction of the hair follicle caused by Trichophyton schonleini Lab Diagnosis Direct Microscopy: 10% KOH is used to digest the skin debris(bottle shaped budding yeast cells) PCR & other molecular methods Direct Microscopy: septate branched hyphae PCR & other molecular methods Culture SDA(Sabourauds dextrose agar medium is used) Creamy colonies appear SDA Honey-combed like thallus(cream colored to
  • 6. Subcutaneous Systemic Definition Infections beneath the skin Infections deep within the body Example & Causative Agent Sporotrichosis: chronic infection of the subcutaneous tissues and adjacent lymphatics characterized by nodular lesions(Sporothrix schenkii) Histoplasmosis (Histoplasma capsulatum): Initial infection in lungs. Later spreads through blood to most organs. Coccidiomycosis (Coccidioides immites): a dimorphic fungus. Resembles tuberculosis Lab Diagnosis Direct Microscopy: Tissue sections should be stained using Grocott's methenamine silver (GMS) Gram stain Interpretation: Look for small narrow base budding yeast cells (2-5um). Clinical material- Skin scrapings, sputum and bronchial washings, cerebrospinal fluid, pleural fluid and blood, bone marrow, urine and tissue biopsies from various visceral organs. 2. Direct Microscopy: Skin scrapings should be examined using 10% KOH and Parker ink or calcofluor white mounts. Exudates and body fluids should be centrifuged and the sediment examined using either 10% KOH
  • 7. FUNGAL DISEASES Opportunistic mycoses: Caused by organisms that are generally harmless unless individual has weakened defenses: ◦ AIDS and cancer patients ◦ Individuals treated with broad spectrum antibiotics ◦ Very old or very young individuals (newborns).  Examples: ◦ Aspergillosis: Inhalation of Aspergillus spores. ◦ Yeast Infections or Candidiasis: Caused mainly by Candida albicans. Part of normal mouth, esophagus, and vaginal flora.
  • 8.  Candidia albicans ◦ a common unicelluar fungus which is part of the flora of the oral cavity, vagina, and gastrointestinal tract; ◦ They become opportunist especially in immunocompromised people  Oral candidiasis (Oral thrush)  Classic thrush is characterised by a white, curd-like coating on the tongue or elsewhere in the oral cavity. Stomatitis due to Candida is often associated with painful infection of the lips - and corners of the mouth.
  • 9.  Genital or perigenital candidiasis ((vulvo vaginitis)  (vulvo)vaginitis due to Candida .  Contamination of the vagina with Candida stems from the endogenous endosaprophytic flora of the gastro- intestinal tract.  Diagnoses ◦ in culture, it grows as blastospores, pseudohyphae, and septate hyphae ◦ candidiasis the collective term for infection involving Candida  cutaneous  vaginal  systemic