Global cooling occurred at the Eocene-Oligocene transition around 34 million years ago, when the warm "greenhouse" climate of the Eocene was replaced by cooler "icehouse" conditions with glaciers forming in Antarctica. Analysis of sedimentary rocks indicates major extinctions in gastropods, amphibians, and reptiles, with little change in mammals. Decreased atmospheric CO2 levels and changes in ocean circulation patterns are implicated as potential causes, with the development of the Antarctic Circumpolar Current helping to sustain Antarctic glaciation. Tooth enamel isotope analysis of mammal fossils provides evidence of an 8°C decrease in mean annual temperature at this transition.