This document summarizes a study that investigated the effects of tocopherol extracted from Lepidium sativum (garden cress) seeds on sperm parameters in male rabbits. The study found that orally administering 33.6 mg/kg of body weight per day of the tocopherol extract for 50 days significantly increased testicular and epididymal sperm concentration, sperm motility, viability and reduced abnormal morphology. This suggests the tocopherol extract from garden cress seeds has the potential to enhance human fertility.
The effect of corn oil in sperm parameters and levels of some hormone, elemen...Alexander Decker
This study investigated the effects of corn oil on sperm parameters, hormone levels, biochemical parameters, and trace elements in male rabbits. Fifteen male rabbits were divided into three groups: a control group given water and two experimental groups given 2 ml/day or 2.5 ml/day of corn oil. The results showed corn oil significantly increased sperm parameters but insignificantly affected hormone levels, biochemical parameters, and trace elements. Corn oil may improve semen quality in animals and humans by optimizing fatty acid levels important for sperm membrane structure and function.
Methimazole affected spermatogenesis and enhanced proliferation of testicular...Jing Zang
This document summarizes a study on the effects of the antithyroid drug methimazole (MMI) on testicular macrophages and spermatogenesis in rats. The study found that treating rats with MMI for 30 days caused a reduction in body weight and seminiferous tubule diameter, and inhibited spermatogenesis. It also observed a significant increase in the number of macrophages in the testicular interstitium, with the highest numbers near Leydig cells. This suggests apoptotic or inflammatory processes increased in the rat testes after MMI exposure. In conclusion, MMI treatment adversely affected testicular macrophages and spermatogenesis in rats, and induced hypothyroidism that can lead to infertility.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
This document describes a study that examined the effects of exogenous melatonin on thyroid hormone levels and melatonin receptor expression in the thyroid glands of male and female mice of different ages. The study found that melatonin treatment inhibited thyroid hormone (T4) levels through unchanged TSH in young male mice but increased both T4 and TSH in older males. In females, melatonin elevated T4 without changing TSH across all ages. Melatonin decreased MT1 receptor expression in both sexes but had varying effects on MT2 receptors depending on age and sex. The findings suggest melatonin differentially regulates T4 in a sex- and age-dependent manner, possibly through
Antigonadotrophic effect of spondias mombin leaf extract in male wistar ratsAlexander Decker
This document summarizes a study that investigated the effects of an aqueous leaf extract of Spondias mombin on male Wistar rats. The rats were divided into three groups: a control group and two experimental groups that received oral doses of 400mg/kg or 800mg/kg of the leaf extract daily for 28 days. After sacrifice, the anterior pituitary glands were examined and serum levels of FSH, LH, and testosterone were measured. The experimental groups showed regression of gonadotropin cells in the anterior pituitary compared to controls. Hormonal assays also revealed significantly lower levels of FSH, LH, and testosterone in the experimental groups. The study concludes that the Spondias mom
Biochemical and pharmacological study of biologically active preparation of p...inventionjournals
Our aim was to perform some biochemical and pharmacological studies of bioactive bovine placental preparation via digestion of cow placenta using enzyme contained in swine stomach. Amino acid compositions and contents in biologically active preparation of placenta, obtained by digestion of cow placenta with enzyme contained in swine stomach were measured by HPLC technique and it was found that contents of such amino acids as glycine, proline and lysine were highest and 9 essential amino acids, including valine, histidine, methionine, lysine, threonine, arginine, phenylalanine, leucine and isoleucine were measured. In pharmacological study, acute toxicity (LD50) of the preparation and effect of the preparation on immune response to sheep erythrocyte were investigated in white mice, weighing 18 to 20 g each. The study revealed acute toxicity (LD50) of the preparation was 60 ml per kg. Spleen index of the first and second experimental group animals treated by the preparation during both provoked and unprovoked immune responses increased by 1.2 to 3.09 times as compared to that of negative control animals, while splenocyte count elevated by 1.2 to 2.2 times than negative control animals. Higher contents of essential amino acids of the biologically active preparation of cattle placenta shows its biologically higher nutritive value, as well as pharmacological study reveals the preparation has minimal toxicity and higher effect to stimulate immune responses.
This study investigated the antioxidant and antimicrobial activities of cow urine and its distillate. The cow urine and distillate were tested for their ability to scavenge free radicals using two in vitro assays and for their antimicrobial activity against several microbes. The results showed that both the cow urine and its distillate exhibited antioxidant and antimicrobial activities by scavenging free radicals and inhibiting the growth of microbes. The fresh cow urine demonstrated stronger antioxidant and antimicrobial effects compared to its distillate. These findings provide scientific support for the traditional use of cow urine as a medicine in India.
Kidney Function Test, Weight Gain and Serum Protein Values of Mature Male Tur...IJEAB
Sixteen sexually matured (12 months old) healthy male turkeys were used to determine the effect of Gonadotrophin (Diclair®) on kidney function, weight gain and serum protein values. The turkeys were divided into 4 treatment groups, identified as T1 (control) administered with 1.00ml physiological saline (0.00 i.u Diclair®), T2 , administered with 13.50 i.u Diclair®, T3,administered with 27.00i.u Dicliar®T4, administered with 40.50 i.u Dicliar(R), with one turkey per replicate in a completely Randomized Design (CRD). The injections were divided into 3 doses each and administered intramuscularly in the thigh for three consecutive days. Blood was collected one week after Diclair® administration. Four turkeys were randomly selected fro-m each treatment groupand bled to collect blood for blood chemistry analysis. The turkey were weighed every week for five weeks and their weight recorded. The result showed that there were significant differences (P< 0.05) among the treatment groups in all parameters for kidney function test: chronicle, potassium, sodium, bicarbonate expect creatinine which was similar (p > 0.05) among the treatment groups. The results further showed that there were no significant differences (p > 0.05) among the treatment groups in initial body weight. However, there were significant differences (P< 0.05) among the treatment groups in final body weight and weight gain. Similarly there were significant differences (P< 0.05) among the treatment groups in all the serum protein values measure: albumin, globulin, serum total protein as well as albumin/globulin ratio. The results of the study showed that Diclair enhanced kidney function and weight gain without any deleterious effects on serum protein values of the male turkeys.
The effect of corn oil in sperm parameters and levels of some hormone, elemen...Alexander Decker
This study investigated the effects of corn oil on sperm parameters, hormone levels, biochemical parameters, and trace elements in male rabbits. Fifteen male rabbits were divided into three groups: a control group given water and two experimental groups given 2 ml/day or 2.5 ml/day of corn oil. The results showed corn oil significantly increased sperm parameters but insignificantly affected hormone levels, biochemical parameters, and trace elements. Corn oil may improve semen quality in animals and humans by optimizing fatty acid levels important for sperm membrane structure and function.
Methimazole affected spermatogenesis and enhanced proliferation of testicular...Jing Zang
This document summarizes a study on the effects of the antithyroid drug methimazole (MMI) on testicular macrophages and spermatogenesis in rats. The study found that treating rats with MMI for 30 days caused a reduction in body weight and seminiferous tubule diameter, and inhibited spermatogenesis. It also observed a significant increase in the number of macrophages in the testicular interstitium, with the highest numbers near Leydig cells. This suggests apoptotic or inflammatory processes increased in the rat testes after MMI exposure. In conclusion, MMI treatment adversely affected testicular macrophages and spermatogenesis in rats, and induced hypothyroidism that can lead to infertility.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
This document describes a study that examined the effects of exogenous melatonin on thyroid hormone levels and melatonin receptor expression in the thyroid glands of male and female mice of different ages. The study found that melatonin treatment inhibited thyroid hormone (T4) levels through unchanged TSH in young male mice but increased both T4 and TSH in older males. In females, melatonin elevated T4 without changing TSH across all ages. Melatonin decreased MT1 receptor expression in both sexes but had varying effects on MT2 receptors depending on age and sex. The findings suggest melatonin differentially regulates T4 in a sex- and age-dependent manner, possibly through
Antigonadotrophic effect of spondias mombin leaf extract in male wistar ratsAlexander Decker
This document summarizes a study that investigated the effects of an aqueous leaf extract of Spondias mombin on male Wistar rats. The rats were divided into three groups: a control group and two experimental groups that received oral doses of 400mg/kg or 800mg/kg of the leaf extract daily for 28 days. After sacrifice, the anterior pituitary glands were examined and serum levels of FSH, LH, and testosterone were measured. The experimental groups showed regression of gonadotropin cells in the anterior pituitary compared to controls. Hormonal assays also revealed significantly lower levels of FSH, LH, and testosterone in the experimental groups. The study concludes that the Spondias mom
Biochemical and pharmacological study of biologically active preparation of p...inventionjournals
Our aim was to perform some biochemical and pharmacological studies of bioactive bovine placental preparation via digestion of cow placenta using enzyme contained in swine stomach. Amino acid compositions and contents in biologically active preparation of placenta, obtained by digestion of cow placenta with enzyme contained in swine stomach were measured by HPLC technique and it was found that contents of such amino acids as glycine, proline and lysine were highest and 9 essential amino acids, including valine, histidine, methionine, lysine, threonine, arginine, phenylalanine, leucine and isoleucine were measured. In pharmacological study, acute toxicity (LD50) of the preparation and effect of the preparation on immune response to sheep erythrocyte were investigated in white mice, weighing 18 to 20 g each. The study revealed acute toxicity (LD50) of the preparation was 60 ml per kg. Spleen index of the first and second experimental group animals treated by the preparation during both provoked and unprovoked immune responses increased by 1.2 to 3.09 times as compared to that of negative control animals, while splenocyte count elevated by 1.2 to 2.2 times than negative control animals. Higher contents of essential amino acids of the biologically active preparation of cattle placenta shows its biologically higher nutritive value, as well as pharmacological study reveals the preparation has minimal toxicity and higher effect to stimulate immune responses.
This study investigated the antioxidant and antimicrobial activities of cow urine and its distillate. The cow urine and distillate were tested for their ability to scavenge free radicals using two in vitro assays and for their antimicrobial activity against several microbes. The results showed that both the cow urine and its distillate exhibited antioxidant and antimicrobial activities by scavenging free radicals and inhibiting the growth of microbes. The fresh cow urine demonstrated stronger antioxidant and antimicrobial effects compared to its distillate. These findings provide scientific support for the traditional use of cow urine as a medicine in India.
Kidney Function Test, Weight Gain and Serum Protein Values of Mature Male Tur...IJEAB
Sixteen sexually matured (12 months old) healthy male turkeys were used to determine the effect of Gonadotrophin (Diclair®) on kidney function, weight gain and serum protein values. The turkeys were divided into 4 treatment groups, identified as T1 (control) administered with 1.00ml physiological saline (0.00 i.u Diclair®), T2 , administered with 13.50 i.u Diclair®, T3,administered with 27.00i.u Dicliar®T4, administered with 40.50 i.u Dicliar(R), with one turkey per replicate in a completely Randomized Design (CRD). The injections were divided into 3 doses each and administered intramuscularly in the thigh for three consecutive days. Blood was collected one week after Diclair® administration. Four turkeys were randomly selected fro-m each treatment groupand bled to collect blood for blood chemistry analysis. The turkey were weighed every week for five weeks and their weight recorded. The result showed that there were significant differences (P< 0.05) among the treatment groups in all parameters for kidney function test: chronicle, potassium, sodium, bicarbonate expect creatinine which was similar (p > 0.05) among the treatment groups. The results further showed that there were no significant differences (p > 0.05) among the treatment groups in initial body weight. However, there were significant differences (P< 0.05) among the treatment groups in final body weight and weight gain. Similarly there were significant differences (P< 0.05) among the treatment groups in all the serum protein values measure: albumin, globulin, serum total protein as well as albumin/globulin ratio. The results of the study showed that Diclair enhanced kidney function and weight gain without any deleterious effects on serum protein values of the male turkeys.
The relationship between progesterone and biochemical constituents of amnioti...Ali Olfati
Ali Olfati1, Gholamali Moghaddam1, Nasroallah Moradi Kor2*, Mitra Bakhtiari3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
2Department of Reproduction Physiologies, Iranian Society of Physiology and Pharmacology, Tehran, Iran
3Department of Anatomical Sciences, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran
Effect of Gonadotrophin (Pergonal®) on Haematological and Serum Biochemical P...Agriculture Journal IJOEAR
Abstract— Twelve Ouda rams aged 2 – 2.6 years and weighed between 40.21 – 40.32kg were randomly distributed into 3 groups of 4 animals with one ram per replicate in a completely randomized design and used to determine the effect of Pergonal® on haematology and serum biochemistry. These groups were assigned to 3 levels of Pergonal® injection as treatments. The injections were 0.00i.u, 49.50i.u, and 99.00i.u Pergonal® represented as T1 (control), T2, and T3, respectively. All the treatments were administered by intramuscular injections. The injections were divided into three doses each and administered intramuscularly in the thigh for three consecutive days. The results of the study showed that apart from Alanine transaminase and eosinophils, the haematological and serum biochemical parameters and immune status of ouda rams may be affected when 49.50i.u or more of Pergonal are used for induction of spermatogenesis. These parameters should be constantly monitored during pergonal administration in ouda rams.
Effcet of Vernonia Amygdalina.Del Ethanolic Extract Fraction on Serum Prolact...iosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
The effects of different levels of Chlorella microalgae on blood biochemical ...Ali Olfati
This document describes a study that investigated the effects of supplementing different levels of Chlorella microalgae on blood parameters and mineral concentrations in laying hens under heat stress conditions. The study found that supplementing 200-500 ppm of Chlorella microalgae decreased serum cholesterol, triglycerides, and LDL levels in the hens, while HDL levels increased. Supplementing 300-500 ppm of Chlorella microalgae also increased plasma levels of manganese, iodine, and selenium in the hens. The results suggest that supplementing Chlorella microalgae at higher rates can benefit the blood parameters of laying hens reared under heat stress.
The Effects of Different Levels Aqueous Extract of Fenugreek (Trigonella foen...Ali Olfati
This study examined the effects of different levels of fenugreek extract in drinking water on performance and immune response in laying hens. 240 laying hens were given 0, 5, 10, or 15 ml/L of fenugreek extract in their water for 6 weeks. Higher levels of fenugreek extract improved egg production, egg mass, and feed conversion ratio. The 15 ml/L treatment significantly increased performance over other treatments. Fenugreek extract also enhanced immune response, with the highest antibody titer seen in hens given 15 ml/L. In conclusion, fenugreek extract supplementation of laying hen drinking water effectively improved both production performance and immune system functioning.
Effect of Ethanolic Extract of Momordica charantia on Blood Sugar Level in No...RahulGupta2015
Using four different experimental models of normal and diabetic male albino rats, blood sugar lowering efficacy of Momordica charantia Linn. of the family Cucurbitaceae has been assessed. Ethanolic (95%) extract of the whole plant of M. charantia significantly lowered blood sugar in fasted, fed and mild diabetic male albino rats at a single oral dose of 250mg/kg that has not been reported earlier. This extract also depressed the peak value significantly in the glucose loaded model.
International Journal of Pharmaceutical Science Invention (IJPSI) inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Ciprofloxacin resideu and their impact on Biomolecules n eggs of laying hens ...Sayed Koushik Ahamed
I have done this research on eggs for the welfare of mankind now i want to share my article for social awareness. I hope it will helps all researchers for their future further research.
Thank You
36.Effect of juvenile hormone on enhanced silk production in Bombyx moriAnnadurai B
The document summarizes research on the effect of juvenile hormone on silk production in Bombyx mori (silkworm). It screened 24 juvenile hormone mimic compounds applied to silkworms at different time periods and concentrations. Several compounds were found to improve economic traits like cocoon weight and shell weight. Specifically, compounds NL-13 and NL-24 increased cocoon weight by 13.22% and shell weight by 23.70% when applied at 5 ppm 48 hours into the 5th instar. Compounds BK and BPE also improved traits like larval weight, cocoon weight, and shell weight up to 22% when applied at certain concentrations and times. The research aims to identify compounds that can enhance
Liver Histological Response of Hyperlipidemic Male Rat (Rattus norvegicus) to...AI Publications
The leaf of lakum (Cayratia trifolia (L.) Domin.) contains compounds that are expectedly antihyperlipidemic. This study aims to determine the liver histological response to the lakum leaf extract and its effect on the levels of SGOT and SGPT in hyperlipidemic male rats (Rattus norvegicus). The male Wistar rats were randomly divided into 4 treatment groups. P0 was a group of rats that were given standard feed, P1 was a group that were fed with high fat diet, P2 was a group that were fed high fat diet and lakum leaf extract with a dose of 40mg/200g BW (body weight)/day, and P3 was a group were fed high fat diet and simvastatin at a dose of 0.18mg/200g BW/day. High fat diet was given for 30 days, whilst treatments were implemented for 28 days. The histological descriptions showed that the hepatocyte cell repair occurred in the treatment group that was given lakum leaf extract but there were no significant differences in liver weight as well as SGOT and SGPT levels. Lakum leaf extract can be used as an antihyperlipidemic agent whilst maintaining the histology of hepatocytes and would not interfere with the liver function of the hyperlipidemic male rats.
DOI: 10.21276/ijlssr.2016.2.3.12
ABSTRACT- Objective: In this experiment adult male albino rats were treated with 50% ethanolic extract of Tephrosia
purpurea fruits at the dose levels of 50, 100 and 200 mg/kg body weight for 60 days, to evaluate antifertility effects in
search of a reversible male contraceptive agent from medicinal plants.
Materials and Methods: Body and organs weight of all treated animals was recorded, blood and serum were analyzed for
hematological indices and clinical biochemistry. To observe effects on reproductive system of animal’s protein, fructose,
sialic acid, ascorbic acid, and glycogen contents were estimated in their testes and sex accessory organs. The treated male
rats were mated with proestrous females and sperm motility, sperm density was determined and FSH, LH and testosterone
hormones were measured to evaluate the effects on fertility. For histopathological observation testes were fixed in Bouin's
fluid, sections were cut at 6 μ and stained with Harris's Haematoxylin and eosin.
Results: Analysis of blood and serum revealed no significant effect after 60 days of the extract treatment. Body weight of
the extract treated rat had no significant alteration, whereas the weight of reproductive organs was decreased significantly
as compared to animals of control group. Protein, sialic acid, fructose contents and level of LH and testosterone hormones
was decreased significantly after treatment in extract treated rats as compared to control.
Conclusions: The fertility, sperm density and motility were declined significantly in rats treated with the ethanolic extract
of Tephrosia purpurea fruits. It is concluded that it might be due to androgen inhibition effects.
Key-words- Antifertility, Tephrosia purpurea, Rat, Testosterone
The results of this study revealed that the use of natural bees’ honey has the ability to protect the liver of rats against the toxic effects of melamine.
Melamine contains 66% nitrogen by mass, so it is sometimes illegally added to food products in order to increase the apparent protein content that has recently become a serious concern. The illegal use of melamine as a food ingredient has led to many poisoning incidents of cats and dogs in the United States, as well as renal function failure of Chinese infants.
Antioxidant and Antitumor Activities on Catunaregum spinosapharmaindexing
The document summarizes a study that evaluated the antioxidant and antitumor activities of the methanol extract of Catunaregum spinosa (MECS) in Dalton's ascites lymphoma (DAL) bearing mice. MECS was administered at doses of 200 and 400 mg/kg for 14 days after tumor inoculation. MECS caused a decrease in tumor volume, viable cell count, and packed cell volume, while increasing lifespan. It also positively impacted hematological and biochemical parameters and increased antioxidant levels. The results suggest MECS has significant antitumor effects in DAL-bearing mice.
Changes in sperm parameters of adult male rabbitsAlexander Decker
1) The study examined the effects of phenol extract from Lepidium sativum seeds on sperm parameters in adult male rabbits.
2) Oral administration of phenol extract at doses of 32, 64, and 96 mg/kg body weight led to a significant increase in sperm concentration and motility in the caudal epididymis compared to the control group.
3) The estimated medium effective dose of phenol extract was 36.1 mg/kg body weight, as it significantly increased testicular sperm concentration, epididymal sperm concentration, sperm count per gram of testis, sperm motility, grade of activity, viability, and abnormal morphology in the caudal epididymis.
This document studied the effects of deer velvet extract from Formosan sika deer on mouse embryonic development and anti-oxidative enzyme expression. Mouse 4-cell embryos were divided into groups and cultured with different concentrations of deer velvet extract or hydrogen peroxide. Embryonic development stages were observed every 12 hours over 72 hours of incubation. The deer velvet extract promoted embryonic development and maintained blastocyst development rates similar to the control when embryos were challenged with hydrogen peroxide. Gene expression of anti-oxidative enzymes in blastocysts was not significantly different between deer velvet treatment groups. The deer velvet extract thus relieved oxidative stress on embryos and supported blastocyst development in vitro.
This document studied the effects of deer velvet extract from Formosan sika deer on mouse embryonic development and anti-oxidative enzyme expression. Mouse 4-cell embryos were divided into groups and cultured with different concentrations of deer velvet extract or hydrogen peroxide. Embryonic development stages were observed every 12 hours over 72 hours of incubation. The deer velvet extract promoted embryonic development and maintained blastocyst development rates similar to the control when embryos were challenged with hydrogen peroxide. Gene expression of anti-oxidative enzymes in blastocysts was not significantly different between deer velvet treatment groups. The deer velvet extract thus relieved oxidative stress on embryos and supported blastocyst development in vitro.
Antinociceptive and Diuretic Activities of Tagetes erecta LinnJing Zang
In the present investigation, the possible antinociceptive and diuretic activities of methanolic extract of Tagetes erecta has been tested in animal models. The methanol extract of both aerial part and root of the plant exhibited significant antinociceptive activity at higher dose (400 mg/kg body weight) in Swiss albino mice. The root extract was found to reduce the writhing more effectively than that of aerial part which is comparable to that produced by aminopyrine, used as standard drug. In addition, crude whole plant extract was also showed efficient diuresis at higher dose 400 mg/kg tested. Diuretic activity was proved by the electrolyte loss ratio (Na+/K+ excretion ratio) and we used furosemide as the reference.
The document describes a study that investigated the effects of ethanolic extract of Garcinia kola seed on reproductive parameters in male Wistar rats. The extract was administered orally at doses of 100 mg/kg and 200 mg/kg for 3 and 6 weeks. Preliminary analysis found the extract contained various phytochemicals and was considered safe up to a dose of 5000 mg/kg. After 3 weeks, testicular weight decreased significantly in treated groups while sperm count and motility increased. After 6 weeks, epididymal weight increased significantly in the high dose group while testicular changes were not significant. The extract appeared to enhance sperm characteristics at both time points without major toxicity.
The document summarizes a study that evaluated the total tannin content and antidiarrheal activity of Tecomaria capensis leaves extract. The study found that the extract contained high quantities of tannins. In tests using animal models, the extract showed significant and dose-dependent inhibition of diarrhea, reduction of intestinal transit, and antienteropooling effects, supporting the traditional use of the plant for treating diarrhea. The results provide scientific evidence that Tecomaria capensis leaves extract has potential antidiarrheal properties.
Hepatoprotective Effect of Cestrum parqui L. aerial parts and Phytochemical ...Jing Zang
This study deals with the investigation of hepatoprotective effect of 70% methanolic extract from Cestrum parqui aerial parts and determination of the bioactive components of the plant. The hepatoprotective effect of Cestrum parqui methanol extract (100, 500, 1000 mg/kg) was analysed on carbon tetrachloride (CCl4)-induced acute liver injury. The administration of a single dose of 40% CCl4 (1ml/kg b.w.) causes an increase in the activities of serum alanine aminotransferase (ALT) and aspirate aminotransferase (AST) enzymes and so pretreated orally of a dose from Cestrum parqui methanol extract (100, 500, 1000 mg/kg) and silymarin (200 mg/kg) for three consecutive days prior to The administration of a single dose of CCl4 significantly prevented the increase in the activities of these enzymes. Histological analysis showed that Cestrum parqui methanol extract at doses of 500 and 1000 mg/kg and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4. The extract cause a negative result on the antioxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd) and decreased malondialdehyde (MDA) level in liver, as compared to those in the CCl4-treated group and this suggests that the hepatoprotective activity of the extract is due to the antioxidant effect of the extract. Phytochemical analysis of the methanol extract from Cestrum parqui aerial parts showed that it contained different phytoconstituents, flavonoids, tannins, saponins, alkaloids, terpenes and carbohydrates.
This study examined the effects of including hempseed at different levels (5%, 10%, 20%) in the diets of Japanese quail on performance, carcass traits, and antioxidant activity in blood. Body weight was not significantly different between groups at 7 and 21 days, but was lower in the 20% hempseed group at 42 days. Feed intake and feed conversion ratio were not significantly different among groups. Carcass, organ weights, and their percentages to carcass weight were significantly different between treatment groups. Biomarkers of oxidative stress (MDA, NO) decreased in blood serum with hempseed supplementation, while antioxidant enzymes (SOD, CAT, GSH-Px) increased, indicating higher antioxidant activity
Effects of Ethanol Extract of Garcinia Kola on Biochemical Markers of Liver F...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
The relationship between progesterone and biochemical constituents of amnioti...Ali Olfati
Ali Olfati1, Gholamali Moghaddam1, Nasroallah Moradi Kor2*, Mitra Bakhtiari3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
2Department of Reproduction Physiologies, Iranian Society of Physiology and Pharmacology, Tehran, Iran
3Department of Anatomical Sciences, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran
Effect of Gonadotrophin (Pergonal®) on Haematological and Serum Biochemical P...Agriculture Journal IJOEAR
Abstract— Twelve Ouda rams aged 2 – 2.6 years and weighed between 40.21 – 40.32kg were randomly distributed into 3 groups of 4 animals with one ram per replicate in a completely randomized design and used to determine the effect of Pergonal® on haematology and serum biochemistry. These groups were assigned to 3 levels of Pergonal® injection as treatments. The injections were 0.00i.u, 49.50i.u, and 99.00i.u Pergonal® represented as T1 (control), T2, and T3, respectively. All the treatments were administered by intramuscular injections. The injections were divided into three doses each and administered intramuscularly in the thigh for three consecutive days. The results of the study showed that apart from Alanine transaminase and eosinophils, the haematological and serum biochemical parameters and immune status of ouda rams may be affected when 49.50i.u or more of Pergonal are used for induction of spermatogenesis. These parameters should be constantly monitored during pergonal administration in ouda rams.
Effcet of Vernonia Amygdalina.Del Ethanolic Extract Fraction on Serum Prolact...iosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
The effects of different levels of Chlorella microalgae on blood biochemical ...Ali Olfati
This document describes a study that investigated the effects of supplementing different levels of Chlorella microalgae on blood parameters and mineral concentrations in laying hens under heat stress conditions. The study found that supplementing 200-500 ppm of Chlorella microalgae decreased serum cholesterol, triglycerides, and LDL levels in the hens, while HDL levels increased. Supplementing 300-500 ppm of Chlorella microalgae also increased plasma levels of manganese, iodine, and selenium in the hens. The results suggest that supplementing Chlorella microalgae at higher rates can benefit the blood parameters of laying hens reared under heat stress.
The Effects of Different Levels Aqueous Extract of Fenugreek (Trigonella foen...Ali Olfati
This study examined the effects of different levels of fenugreek extract in drinking water on performance and immune response in laying hens. 240 laying hens were given 0, 5, 10, or 15 ml/L of fenugreek extract in their water for 6 weeks. Higher levels of fenugreek extract improved egg production, egg mass, and feed conversion ratio. The 15 ml/L treatment significantly increased performance over other treatments. Fenugreek extract also enhanced immune response, with the highest antibody titer seen in hens given 15 ml/L. In conclusion, fenugreek extract supplementation of laying hen drinking water effectively improved both production performance and immune system functioning.
Effect of Ethanolic Extract of Momordica charantia on Blood Sugar Level in No...RahulGupta2015
Using four different experimental models of normal and diabetic male albino rats, blood sugar lowering efficacy of Momordica charantia Linn. of the family Cucurbitaceae has been assessed. Ethanolic (95%) extract of the whole plant of M. charantia significantly lowered blood sugar in fasted, fed and mild diabetic male albino rats at a single oral dose of 250mg/kg that has not been reported earlier. This extract also depressed the peak value significantly in the glucose loaded model.
International Journal of Pharmaceutical Science Invention (IJPSI) inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Ciprofloxacin resideu and their impact on Biomolecules n eggs of laying hens ...Sayed Koushik Ahamed
I have done this research on eggs for the welfare of mankind now i want to share my article for social awareness. I hope it will helps all researchers for their future further research.
Thank You
36.Effect of juvenile hormone on enhanced silk production in Bombyx moriAnnadurai B
The document summarizes research on the effect of juvenile hormone on silk production in Bombyx mori (silkworm). It screened 24 juvenile hormone mimic compounds applied to silkworms at different time periods and concentrations. Several compounds were found to improve economic traits like cocoon weight and shell weight. Specifically, compounds NL-13 and NL-24 increased cocoon weight by 13.22% and shell weight by 23.70% when applied at 5 ppm 48 hours into the 5th instar. Compounds BK and BPE also improved traits like larval weight, cocoon weight, and shell weight up to 22% when applied at certain concentrations and times. The research aims to identify compounds that can enhance
Liver Histological Response of Hyperlipidemic Male Rat (Rattus norvegicus) to...AI Publications
The leaf of lakum (Cayratia trifolia (L.) Domin.) contains compounds that are expectedly antihyperlipidemic. This study aims to determine the liver histological response to the lakum leaf extract and its effect on the levels of SGOT and SGPT in hyperlipidemic male rats (Rattus norvegicus). The male Wistar rats were randomly divided into 4 treatment groups. P0 was a group of rats that were given standard feed, P1 was a group that were fed with high fat diet, P2 was a group that were fed high fat diet and lakum leaf extract with a dose of 40mg/200g BW (body weight)/day, and P3 was a group were fed high fat diet and simvastatin at a dose of 0.18mg/200g BW/day. High fat diet was given for 30 days, whilst treatments were implemented for 28 days. The histological descriptions showed that the hepatocyte cell repair occurred in the treatment group that was given lakum leaf extract but there were no significant differences in liver weight as well as SGOT and SGPT levels. Lakum leaf extract can be used as an antihyperlipidemic agent whilst maintaining the histology of hepatocytes and would not interfere with the liver function of the hyperlipidemic male rats.
DOI: 10.21276/ijlssr.2016.2.3.12
ABSTRACT- Objective: In this experiment adult male albino rats were treated with 50% ethanolic extract of Tephrosia
purpurea fruits at the dose levels of 50, 100 and 200 mg/kg body weight for 60 days, to evaluate antifertility effects in
search of a reversible male contraceptive agent from medicinal plants.
Materials and Methods: Body and organs weight of all treated animals was recorded, blood and serum were analyzed for
hematological indices and clinical biochemistry. To observe effects on reproductive system of animal’s protein, fructose,
sialic acid, ascorbic acid, and glycogen contents were estimated in their testes and sex accessory organs. The treated male
rats were mated with proestrous females and sperm motility, sperm density was determined and FSH, LH and testosterone
hormones were measured to evaluate the effects on fertility. For histopathological observation testes were fixed in Bouin's
fluid, sections were cut at 6 μ and stained with Harris's Haematoxylin and eosin.
Results: Analysis of blood and serum revealed no significant effect after 60 days of the extract treatment. Body weight of
the extract treated rat had no significant alteration, whereas the weight of reproductive organs was decreased significantly
as compared to animals of control group. Protein, sialic acid, fructose contents and level of LH and testosterone hormones
was decreased significantly after treatment in extract treated rats as compared to control.
Conclusions: The fertility, sperm density and motility were declined significantly in rats treated with the ethanolic extract
of Tephrosia purpurea fruits. It is concluded that it might be due to androgen inhibition effects.
Key-words- Antifertility, Tephrosia purpurea, Rat, Testosterone
The results of this study revealed that the use of natural bees’ honey has the ability to protect the liver of rats against the toxic effects of melamine.
Melamine contains 66% nitrogen by mass, so it is sometimes illegally added to food products in order to increase the apparent protein content that has recently become a serious concern. The illegal use of melamine as a food ingredient has led to many poisoning incidents of cats and dogs in the United States, as well as renal function failure of Chinese infants.
Antioxidant and Antitumor Activities on Catunaregum spinosapharmaindexing
The document summarizes a study that evaluated the antioxidant and antitumor activities of the methanol extract of Catunaregum spinosa (MECS) in Dalton's ascites lymphoma (DAL) bearing mice. MECS was administered at doses of 200 and 400 mg/kg for 14 days after tumor inoculation. MECS caused a decrease in tumor volume, viable cell count, and packed cell volume, while increasing lifespan. It also positively impacted hematological and biochemical parameters and increased antioxidant levels. The results suggest MECS has significant antitumor effects in DAL-bearing mice.
Changes in sperm parameters of adult male rabbitsAlexander Decker
1) The study examined the effects of phenol extract from Lepidium sativum seeds on sperm parameters in adult male rabbits.
2) Oral administration of phenol extract at doses of 32, 64, and 96 mg/kg body weight led to a significant increase in sperm concentration and motility in the caudal epididymis compared to the control group.
3) The estimated medium effective dose of phenol extract was 36.1 mg/kg body weight, as it significantly increased testicular sperm concentration, epididymal sperm concentration, sperm count per gram of testis, sperm motility, grade of activity, viability, and abnormal morphology in the caudal epididymis.
This document studied the effects of deer velvet extract from Formosan sika deer on mouse embryonic development and anti-oxidative enzyme expression. Mouse 4-cell embryos were divided into groups and cultured with different concentrations of deer velvet extract or hydrogen peroxide. Embryonic development stages were observed every 12 hours over 72 hours of incubation. The deer velvet extract promoted embryonic development and maintained blastocyst development rates similar to the control when embryos were challenged with hydrogen peroxide. Gene expression of anti-oxidative enzymes in blastocysts was not significantly different between deer velvet treatment groups. The deer velvet extract thus relieved oxidative stress on embryos and supported blastocyst development in vitro.
This document studied the effects of deer velvet extract from Formosan sika deer on mouse embryonic development and anti-oxidative enzyme expression. Mouse 4-cell embryos were divided into groups and cultured with different concentrations of deer velvet extract or hydrogen peroxide. Embryonic development stages were observed every 12 hours over 72 hours of incubation. The deer velvet extract promoted embryonic development and maintained blastocyst development rates similar to the control when embryos were challenged with hydrogen peroxide. Gene expression of anti-oxidative enzymes in blastocysts was not significantly different between deer velvet treatment groups. The deer velvet extract thus relieved oxidative stress on embryos and supported blastocyst development in vitro.
Antinociceptive and Diuretic Activities of Tagetes erecta LinnJing Zang
In the present investigation, the possible antinociceptive and diuretic activities of methanolic extract of Tagetes erecta has been tested in animal models. The methanol extract of both aerial part and root of the plant exhibited significant antinociceptive activity at higher dose (400 mg/kg body weight) in Swiss albino mice. The root extract was found to reduce the writhing more effectively than that of aerial part which is comparable to that produced by aminopyrine, used as standard drug. In addition, crude whole plant extract was also showed efficient diuresis at higher dose 400 mg/kg tested. Diuretic activity was proved by the electrolyte loss ratio (Na+/K+ excretion ratio) and we used furosemide as the reference.
The document describes a study that investigated the effects of ethanolic extract of Garcinia kola seed on reproductive parameters in male Wistar rats. The extract was administered orally at doses of 100 mg/kg and 200 mg/kg for 3 and 6 weeks. Preliminary analysis found the extract contained various phytochemicals and was considered safe up to a dose of 5000 mg/kg. After 3 weeks, testicular weight decreased significantly in treated groups while sperm count and motility increased. After 6 weeks, epididymal weight increased significantly in the high dose group while testicular changes were not significant. The extract appeared to enhance sperm characteristics at both time points without major toxicity.
The document summarizes a study that evaluated the total tannin content and antidiarrheal activity of Tecomaria capensis leaves extract. The study found that the extract contained high quantities of tannins. In tests using animal models, the extract showed significant and dose-dependent inhibition of diarrhea, reduction of intestinal transit, and antienteropooling effects, supporting the traditional use of the plant for treating diarrhea. The results provide scientific evidence that Tecomaria capensis leaves extract has potential antidiarrheal properties.
Hepatoprotective Effect of Cestrum parqui L. aerial parts and Phytochemical ...Jing Zang
This study deals with the investigation of hepatoprotective effect of 70% methanolic extract from Cestrum parqui aerial parts and determination of the bioactive components of the plant. The hepatoprotective effect of Cestrum parqui methanol extract (100, 500, 1000 mg/kg) was analysed on carbon tetrachloride (CCl4)-induced acute liver injury. The administration of a single dose of 40% CCl4 (1ml/kg b.w.) causes an increase in the activities of serum alanine aminotransferase (ALT) and aspirate aminotransferase (AST) enzymes and so pretreated orally of a dose from Cestrum parqui methanol extract (100, 500, 1000 mg/kg) and silymarin (200 mg/kg) for three consecutive days prior to The administration of a single dose of CCl4 significantly prevented the increase in the activities of these enzymes. Histological analysis showed that Cestrum parqui methanol extract at doses of 500 and 1000 mg/kg and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4. The extract cause a negative result on the antioxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd) and decreased malondialdehyde (MDA) level in liver, as compared to those in the CCl4-treated group and this suggests that the hepatoprotective activity of the extract is due to the antioxidant effect of the extract. Phytochemical analysis of the methanol extract from Cestrum parqui aerial parts showed that it contained different phytoconstituents, flavonoids, tannins, saponins, alkaloids, terpenes and carbohydrates.
This study examined the effects of including hempseed at different levels (5%, 10%, 20%) in the diets of Japanese quail on performance, carcass traits, and antioxidant activity in blood. Body weight was not significantly different between groups at 7 and 21 days, but was lower in the 20% hempseed group at 42 days. Feed intake and feed conversion ratio were not significantly different among groups. Carcass, organ weights, and their percentages to carcass weight were significantly different between treatment groups. Biomarkers of oxidative stress (MDA, NO) decreased in blood serum with hempseed supplementation, while antioxidant enzymes (SOD, CAT, GSH-Px) increased, indicating higher antioxidant activity
Effects of Ethanol Extract of Garcinia Kola on Biochemical Markers of Liver F...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Evaluation of antinociceptive effect of Terminalia arjuna bark ethanol extractOpen Access Research Paper
Stem bark of the plant Terminalia arjuna were extracted in 50% ethanol to evaluate for centrally acting analgesic potential using formalin, hot plate and peripheral pharmacological actions using acetic acid induced writhing test in mice. The extract of the plant were found to have significant (p<0.01) analgesic activity at the oral dose of 250 & 500 mg/kg body weight, in the tested models. In hot plate test, at both dose levels (250mg/kg and 500mg/kg), T. arjuna extract showed significant (p<0.001) increased latency period than the control group. In acetic acid induced writhing test and formalin test T. arjuna also showed reduced number of writhes than the control group at two dose levels which are significant (p<0.05) compared to control. The results obtained support the use of stem bark of T.arjuna in painful conditions acting both centrally and peripherally.
The study investigated the effects of dietary supplementation with coriander seeds on food intake and microbial populations in the ceca of Japanese quail. 360 female quail chicks were divided into 6 treatment groups with varying levels of coriander seed supplementation (0-4%) or an antibiotic control. Food intake was measured weekly for 5 weeks, and bacterial populations in the ceca were analyzed after 35 days. Results showed that groups with 1% and 2% coriander supplementation had the highest food intake in the first two weeks. Bacterial analysis found the 4% coriander group had the highest E. coli levels, while the antibiotic group had the lowest levels. The study suggests coriander seeds can increase early food intake
Biochemical changes induced by Bioneem (0.03%) formulation in chick embryogen...Agriculture Journal IJOEAR
Abstract— In ovo studies on the effect of 1,3,5, ppm Bioneem (0.03%) formulation on Biochemical aspect of chick embryo revealed that there was dose dependent total protein reduction in 96 hrs old embryo (treated at 24 hrs) as compared to the control. Also there was reduction in total protein concentration Liver, Brain and Heart of 15 day old chick embryo (treated with Bioneem at 96 hrs. stage) as compared to that of control. Protein carbonyl concentration of 96 hrs old embryo (treated at 24 hrs with Bioneem) and that of Liver, Brain and Heart of 15 day old chick embryo (treated with bioneem at 96 hrs) increased in dose dependent manner. Most affected organ was Liver and least affected organ was Heart. Blood analysis of 15 day old chick embryo (treated with Bioneem at 96 hrs) showed increased level of Blood urea, LDH, SGOT, SGPT, while Serum alkaline phosphatase and serum cholesterol were decreased in dose dependent manner as compared to the control. Thus Bioneem though ecofriendly pesticide can adversely affect vertebrate non target organisms and therefore should be carefully used in pest management programs.
This research article studied the anti-obesity effects of Moringa oleifera leaf extract (MEMOL) in rats with high fat diet-induced obesity. Rats were fed a high fat diet to induce obesity over 49 days. Treatment with MEMOL for 49 days significantly reduced body weight, total cholesterol, triglycerides, and LDL levels in obese rats, and increased body temperature, compared to untreated obese rats. MEMOL treated rats also showed decreased levels of liver enzymes and blood glucose. The results indicate that MEMOL attenuated body weight gain in obese rats without affecting food intake, and demonstrated hypoglycemic and hypolipidemic effects, suggesting it may help treat obesity and related disorders.
This research article studied the anti-obesity effects of Moringa oleifera leaf extract (MEMOL) in rats fed a high-fat diet. Rats fed the high-fat diet for 49 days became obese, with increased body weight, total cholesterol, triglycerides, and lowered HDL levels. Treatment with MEMOL at doses of 200 mg/kg and 400 mg/kg for 49 days significantly reduced body weight gain, total cholesterol, triglycerides, and LDL levels in obese rats compared to those fed just the high-fat diet. MEMOL treatment also increased body temperature and lowered liver enzymes, organ weights, and blood glucose levels in obese rats. The results suggest that MEMOL has anti-ob
Effects of Metformin, Pioglitazone and Aqueous Extract of Delonix Regia on Bl...iosrjce
The effects of Delonix regia extract (d200mg, d300mg, and d400mg), metformin (m8.3mg, m12.5mg
and m16.5mg), pioglitazone (p0.5mg, p0.7mg and p0.9mg) and combined formulation of metformin and extract
(m6.25d150mg) on glycated hemoglobin status in streptozotocin-induced diabetic Albino wistar rats. Diabetic
status of these rats was assessed by estimating fasting blood glucose levels. A total of 150 albino rats were used
for the investigation and were grouped into twelve groups of twelve rats each as follows; Group I: normal
control rats (NCR). Group II: Diabetic control rats (DCR). Group III: Diabetic rats treated with d200mg.
Group IV: Diabetic rats treated with d300mg. Group V: Diabetic rats treated with d400mg. Group VI: Diabetic
rats treated with m8.3mg. Group VII: Diabetic rats treated with m12.5mg. Group VIII: Diabetic rats treated
with m16.5mg. Group IX: Diabetic rats treated with p0.5mg. Group X: Diabetic rats treated with p0.75mg.
Group XI: Diabetic rats treated with p1.0mg. Group XII: Diabetic rats treated with m125d300mg each for male
and female respectively, for a total of 56 days. After every two weeks interval of treatment for eight weeks three
rats from each group were sacrificed and blood sample were collected and analyzed for various parameters.
The result obtained showed an elevated level of glycated hemoglobin in diabetic-induced wistar albino rats
compared with normal control rats. However, there was reversal of the effects when treated with the
drug/extract. Also there was reduction in the blood glucose level of the diabetic rats treated with metformin
(from 6.37±0.69 to 5.20±0.62mmol/l), pioglitazone (from 7.30±0.21mmol/l to 4.70±0.46), aqueous extract of
Delonixregia (from 8.20±0.81mmol/l to 6.10±0.60) and combined formulation of metformin and extract (from
7.81±0.34 to 4.80±0.17), at p<0.05 confidence level when compared with diabetic control rats in the various
weeks of treatment respectively
This study examined the effects of di-(2-ethylhexyl) phthalate (DEHP) exposure on the uterus of adult female rats. Rats were orally administered DEHP at doses of 0, 1, 10, and 100 mg/kg body weight daily for 30 days. Key findings include:
1) Serum estradiol levels were unchanged in the 1 and 10 mg groups but marginally increased in the 100 mg group. Progesterone levels increased in the 1 and 10 mg groups.
2) Histological examination found structural abnormalities in the uterus such as decreased diameter and thinning of layers in the 10 and 100 mg groups.
3) mRNA expression of estrogen receptor alpha decreased in the 100 mg
Characterization of jec absorbed compounds in lactating rat's milk and suckli...Norhafilda Ismail
Jamu Ratus, a widespread use of traditional complementary herbal remedies upon post-partum recovery. The herbal remedies constituents may comprising abundance of herbs and spices namely Piper sp., Alpinia sp., Kaempferia galangal, Curcuma sp. which representing the complexity of herbal formulas and the presence of ubiquitous of bioactive compounds. Preliminary studies has been conducted using different concentrations of therapeutic dosages as given; 0mg/kg/day as control; 150mg/kg/day; 500mg/kg/day and 1500mg/kg/day in lactating rat’s milk and suckling neonates after giving birth within 1 month upon gestation period. The main objectives is to characterize the absorbed of JEC compounds derived from ethanolic fractions compounds using lactating’ rats milks and plasma tissues from liver and brain’s (n=30) of suckling neonates. The subjected samples tested were collected, weighed and further analysed using Thin Layer Chromatography (TLC); High Performance of Chromatography (HPLC) and Liquid Chromatography Mass Spectrometry (LCMS) using different phase of solvent as eluents. The presence of flavonoids compounds even at lower dosage of therapeutic dosage (150mg/kg/day) given in neonatal tissues brain persistent with time and dosage given; giving good insight in understanding of xenobiotic metabolism in the liver. The bioaccumulation of absorbed JEC compounds in neonatal brain and liver tissues showing the bioavailability of the drug itself to retain, persistent with time of drug exposure. Albeit the vulnerability of the neonatal liver and brain in early stage development upon drug interference to transverse into the brain tissues; thus, implicated the toxicity potential whilst exhibits and affecting cognitive development and physiological activities and henceforth need to be further resolved.
Key Words;, JEC compounds, xenobiotic , TLC, HPLC and LCMS.
Characterization of jec absorbed compounds in lactating rat's milk and suckli...Norhafilda Ismail
Jamu Ratus, a widespread use of traditional complementary herbal remedies upon post-partum recovery. The herbal remedies constituents may comprising abundance of herbs and spices namely Piper sp., Alpinia sp., Kaempferia galangal, Curcuma sp. which representing the complexity of herbal formulas and the presence of ubiquitous of bioactive compounds. Preliminary studies has been conducted using different concentrations of therapeutic dosages as given; 0mg/kg/day as control; 150mg/kg/day; 500mg/kg/day and 1500mg/kg/day in lactating rat’s milk and suckling neonates after giving birth within 1 month upon gestation period. The main objectives is to characterize the absorbed of JEC compounds derived from ethanolic fractions compounds using lactating’ rats milks and plasma tissues from liver and brain’s (n=30) of suckling neonates. The subjected samples tested were collected, weighed and further analysed using Thin Layer Chromatography (TLC); High Performance of Chromatography (HPLC) and Liquid Chromatography Mass Spectrometry (LCMS) using different phase of solvent as eluents. The presence of flavonoids compounds even at lower dosage of therapeutic dosage (150mg/kg/day) given in neonatal tissues brain persistent with time and dosage given; giving good insight in understanding of xenobiotic metabolism in the liver. The bioaccumulation of absorbed JEC compounds in neonatal brain and liver tissues showing the bioavailability of the drug itself to retain, persistent with time of drug exposure. Albeit the vulnerability of the neonatal liver and brain in early stage development upon drug interference to transverse into the brain tissues; thus, implicated the toxicity potential whilst exhibits and affecting cognitive development and physiological activities and henceforth need to be further resolved.
Key Words;, JEC compounds, xenobiotic , TLC, HPLC and LCMS.
2.a OPTIMIZATION CHARACTERIZATION OF JEC ABSORBED COMPOUNDS IN LACTATING RATS...Norhafilda Ismail
This document summarizes a study that characterized compounds from Jamu Ratus, a traditional Malaysian herbal remedy, absorbed in the milk of lactating rats and tissues of suckling neonates. Lactating rats were orally administered Jamu Ratus ethanolic extract (JEC) at different dosages and their milk and neonate tissues were analyzed. Thin layer chromatography, high performance liquid chromatography, and liquid chromatography-mass spectrometry identified flavonoid compounds in neonatal tissues, even at low dosages, indicating bioavailability and persistence over time. While herbal remedies are traditionally claimed to benefit postpartum recovery, this study found the absorbed JEC compounds bioaccumulated in neonatal liver and brain tissues, implicating potential
Screening Fenugreek genotypes for high callus induction and growth | IJAARInnspub Net
Fenugreek (Trigonella foenume-graecum) is a medicinal herb that has anti-diabetic properties. 4-hydroxyisoleucine, an important metabolite of this plant, is a good candidate for improving hepatic insulin resistance in type 2 diabetic patients. In vitro production of metabolites mainly relies on the ability of the tissue culture system to produce more and viable cells with maximum production rate. Callus induction is the first step of establishing plant cell based bioreactors which needs potential genotypes with higher and faster callus production. To find such genotypes, screening them is indispensable. In this research, we screened 21 Iranian landraces of fenugreek for callus induction and growth on MS medium supplemented with some plant growth regulators. The results showed different capacity of the screened genotypes in callus induction (P<0.05). Also, the type of explant had a significant effect on callus induction (P<0.05). Calli were able to produce 4-Hydroxyisulosine 67% of the amount in vivo samples.
Similar to Effect of tocopherol extraction of lepidium sativum seeds in sperm parameters of white male rabbits (20)
Abnormalities of hormones and inflammatory cytokines in women affected with p...Alexander Decker
Women with polycystic ovary syndrome (PCOS) have elevated levels of hormones like luteinizing hormone and testosterone, as well as higher levels of insulin and insulin resistance compared to healthy women. They also have increased levels of inflammatory markers like C-reactive protein, interleukin-6, and leptin. This study found these abnormalities in the hormones and inflammatory cytokines of women with PCOS ages 23-40, indicating that hormone imbalances associated with insulin resistance and elevated inflammatory markers may worsen infertility in women with PCOS.
A usability evaluation framework for b2 c e commerce websitesAlexander Decker
This document presents a framework for evaluating the usability of B2C e-commerce websites. It involves user testing methods like usability testing and interviews to identify usability problems in areas like navigation, design, purchasing processes, and customer service. The framework specifies goals for the evaluation, determines which website aspects to evaluate, and identifies target users. It then describes collecting data through user testing and analyzing the results to identify usability problems and suggest improvements.
A universal model for managing the marketing executives in nigerian banksAlexander Decker
This document discusses a study that aimed to synthesize motivation theories into a universal model for managing marketing executives in Nigerian banks. The study was guided by Maslow and McGregor's theories. A sample of 303 marketing executives was used. The results showed that managers will be most effective at motivating marketing executives if they consider individual needs and create challenging but attainable goals. The emerged model suggests managers should provide job satisfaction by tailoring assignments to abilities and monitoring performance with feedback. This addresses confusion faced by Nigerian bank managers in determining effective motivation strategies.
A unique common fixed point theorems in generalized dAlexander Decker
This document presents definitions and properties related to generalized D*-metric spaces and establishes some common fixed point theorems for contractive type mappings in these spaces. It begins by introducing D*-metric spaces and generalized D*-metric spaces, defines concepts like convergence and Cauchy sequences. It presents lemmas showing the uniqueness of limits in these spaces and the equivalence of different definitions of convergence. The goal of the paper is then stated as obtaining a unique common fixed point theorem for generalized D*-metric spaces.
A trends of salmonella and antibiotic resistanceAlexander Decker
This document provides a review of trends in Salmonella and antibiotic resistance. It begins with an introduction to Salmonella as a facultative anaerobe that causes nontyphoidal salmonellosis. The emergence of antimicrobial-resistant Salmonella is then discussed. The document proceeds to cover the historical perspective and classification of Salmonella, definitions of antimicrobials and antibiotic resistance, and mechanisms of antibiotic resistance in Salmonella including modification or destruction of antimicrobial agents, efflux pumps, modification of antibiotic targets, and decreased membrane permeability. Specific resistance mechanisms are discussed for several classes of antimicrobials.
A transformational generative approach towards understanding al-istifhamAlexander Decker
This document discusses a transformational-generative approach to understanding Al-Istifham, which refers to interrogative sentences in Arabic. It begins with an introduction to the origin and development of Arabic grammar. The paper then explains the theoretical framework of transformational-generative grammar that is used. Basic linguistic concepts and terms related to Arabic grammar are defined. The document analyzes how interrogative sentences in Arabic can be derived and transformed via tools from transformational-generative grammar, categorizing Al-Istifham into linguistic and literary questions.
A time series analysis of the determinants of savings in namibiaAlexander Decker
This document summarizes a study on the determinants of savings in Namibia from 1991 to 2012. It reviews previous literature on savings determinants in developing countries. The study uses time series analysis including unit root tests, cointegration, and error correction models to analyze the relationship between savings and variables like income, inflation, population growth, deposit rates, and financial deepening in Namibia. The results found inflation and income have a positive impact on savings, while population growth negatively impacts savings. Deposit rates and financial deepening were found to have no significant impact. The study reinforces previous work and emphasizes the importance of improving income levels to achieve higher savings rates in Namibia.
A therapy for physical and mental fitness of school childrenAlexander Decker
This document summarizes a study on the importance of exercise in maintaining physical and mental fitness for school children. It discusses how physical and mental fitness are developed through participation in regular physical exercises and cannot be achieved solely through classroom learning. The document outlines different types and components of fitness and argues that developing fitness should be a key objective of education systems. It recommends that schools ensure pupils engage in graded physical activities and exercises to support their overall development.
A theory of efficiency for managing the marketing executives in nigerian banksAlexander Decker
This document summarizes a study examining efficiency in managing marketing executives in Nigerian banks. The study was examined through the lenses of Kaizen theory (continuous improvement) and efficiency theory. A survey of 303 marketing executives from Nigerian banks found that management plays a key role in identifying and implementing efficiency improvements. The document recommends adopting a "3H grand strategy" to improve the heads, hearts, and hands of management and marketing executives by enhancing their knowledge, attitudes, and tools.
This document discusses evaluating the link budget for effective 900MHz GSM communication. It describes the basic parameters needed for a high-level link budget calculation, including transmitter power, antenna gains, path loss, and propagation models. Common propagation models for 900MHz that are described include Okumura model for urban areas and Hata model for urban, suburban, and open areas. Rain attenuation is also incorporated using the updated ITU model to improve communication during rainfall.
A synthetic review of contraceptive supplies in punjabAlexander Decker
This document discusses contraceptive use in Punjab, Pakistan. It begins by providing background on the benefits of family planning and contraceptive use for maternal and child health. It then analyzes contraceptive commodity data from Punjab, finding that use is still low despite efforts to improve access. The document concludes by emphasizing the need for strategies to bridge gaps and meet the unmet need for effective and affordable contraceptive methods and supplies in Punjab in order to improve health outcomes.
A synthesis of taylor’s and fayol’s management approaches for managing market...Alexander Decker
1) The document discusses synthesizing Taylor's scientific management approach and Fayol's process management approach to identify an effective way to manage marketing executives in Nigerian banks.
2) It reviews Taylor's emphasis on efficiency and breaking tasks into small parts, and Fayol's focus on developing general management principles.
3) The study administered a survey to 303 marketing executives in Nigerian banks to test if combining elements of Taylor and Fayol's approaches would help manage their performance through clear roles, accountability, and motivation. Statistical analysis supported combining the two approaches.
A survey paper on sequence pattern mining with incrementalAlexander Decker
This document summarizes four algorithms for sequential pattern mining: GSP, ISM, FreeSpan, and PrefixSpan. GSP is an Apriori-based algorithm that incorporates time constraints. ISM extends SPADE to incrementally update patterns after database changes. FreeSpan uses frequent items to recursively project databases and grow subsequences. PrefixSpan also uses projection but claims to not require candidate generation. It recursively projects databases based on short prefix patterns. The document concludes by stating the goal was to find an efficient scheme for extracting sequential patterns from transactional datasets.
A survey on live virtual machine migrations and its techniquesAlexander Decker
This document summarizes several techniques for live virtual machine migration in cloud computing. It discusses works that have proposed affinity-aware migration models to improve resource utilization, energy efficient migration approaches using storage migration and live VM migration, and a dynamic consolidation technique using migration control to avoid unnecessary migrations. The document also summarizes works that have designed methods to minimize migration downtime and network traffic, proposed a resource reservation framework for efficient migration of multiple VMs, and addressed real-time issues in live migration. Finally, it provides a table summarizing the techniques, tools used, and potential future work or gaps identified for each discussed work.
A survey on data mining and analysis in hadoop and mongo dbAlexander Decker
This document discusses data mining of big data using Hadoop and MongoDB. It provides an overview of Hadoop and MongoDB and their uses in big data analysis. Specifically, it proposes using Hadoop for distributed processing and MongoDB for data storage and input. The document reviews several related works that discuss big data analysis using these tools, as well as their capabilities for scalable data storage and mining. It aims to improve computational time and fault tolerance for big data analysis by mining data stored in Hadoop using MongoDB and MapReduce.
1. The document discusses several challenges for integrating media with cloud computing including media content convergence, scalability and expandability, finding appropriate applications, and reliability.
2. Media content convergence challenges include dealing with the heterogeneity of media types, services, networks, devices, and quality of service requirements as well as integrating technologies used by media providers and consumers.
3. Scalability and expandability challenges involve adapting to the increasing volume of media content and being able to support new media formats and outlets over time.
This document surveys trust architectures that leverage provenance in wireless sensor networks. It begins with background on provenance, which refers to the documented history or derivation of data. Provenance can be used to assess trust by providing metadata about how data was processed. The document then discusses challenges for using provenance to establish trust in wireless sensor networks, which have constraints on energy and computation. Finally, it provides background on trust, which is the subjective probability that a node will behave dependably. Trust architectures need to be lightweight to account for the constraints of wireless sensor networks.
This document discusses private equity investments in Kenya. It provides background on private equity and discusses trends in various regions. The objectives of the study discussed are to establish the extent of private equity adoption in Kenya, identify common forms of private equity utilized, and determine typical exit strategies. Private equity can involve venture capital, leveraged buyouts, or mezzanine financing. Exits allow recycling of capital into new opportunities. The document provides context on private equity globally and in developing markets like Africa to frame the goals of the study.
This document discusses a study that analyzes the financial health of the Indian logistics industry from 2005-2012 using Altman's Z-score model. The study finds that the average Z-score for selected logistics firms was in the healthy to very healthy range during the study period. The average Z-score increased from 2006 to 2010 when the Indian economy was hit by the global recession, indicating the overall performance of the Indian logistics industry was good. The document reviews previous literature on measuring financial performance and distress using ratios and Z-scores, and outlines the objectives and methodology used in the current study.
Adhd Medication Shortage Uk - trinexpharmacy.comreignlana06
The UK is currently facing a Adhd Medication Shortage Uk, which has left many patients and their families grappling with uncertainty and frustration. ADHD, or Attention Deficit Hyperactivity Disorder, is a chronic condition that requires consistent medication to manage effectively. This shortage has highlighted the critical role these medications play in the daily lives of those affected by ADHD. Contact : +1 (747) 209 – 3649 E-mail : sales@trinexpharmacy.com
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
Does Over-Masturbation Contribute to Chronic Prostatitis.pptxwalterHu5
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Effect of tocopherol extraction of lepidium sativum seeds in sperm parameters of white male rabbits
1. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.3, No.8, 2013
43
Effect of Tocopherol Extraction of Lepidium Sativum Seeds in
Sperm Parameters of White Male Rabbits
Nada Saad Naji 1
* Faris Naji Abood 2
1. Department of General Sciences, College of Basic Education, University of Babylon, PO box 4, Iraq
2. Department of Biology, College of Science, University of Babylon, PO box 4, Iraq
* E-mail of the corresponding author: Dr_n_saad_Altaae@yahoo.com
Abstract
The objective of this research was to investigate the effect of tocopherol extraction of Lepidium sativum seeds on
the fertility of adult male rabbit. The In Vivo study included the estimation of the Medium Effect Dose (MED50)
for tocopherol extraction by using Dose-Response Curve. The mean of MED50 was 33.6 mg/kg body weight
(b.w.) of tocopherol. The results showed a significant increase (P< 0.05) in testicular sperm concentration,
epididymus sperm concentration and in the sperm count per gm of the testis, sperm motility percent, grade
activity, sperm viability percent. While it was found a decrease in abnormal sperm morphology percent of
epididymis caudal at MED50 of tocopherol. Our results reveal that due to tocopherol administration; male rabbits
fertility increases, thus being useful to any animal, mammals as well as human being.
Keywords: Lepidium sativum, seeds, tocopherol, rabbits, sperm parameters
1.Introduction
Lepidium sativum (family Brassicaceae) locally known as ‘garden cress’ has been used widely in different parts
of the world for its wide therapeutic application, a number of recent studies pointed out the traditional uses of
Lepidium sativum seeds extract in controlling many clinical problems (Gill & MacLeod 1980), and (Ahmed et al.
2013) revealed that Lepidium sativum seeds with high nutritional value can be exploited as a functional food
ingredient, and Garden cress meal is a by-product remaining after the extraction of the oil from seeds. It is
usually cultivated for its leaves, which are used in salad, sandwiches etc. (Lefroy Valley carries 2013) .The
leaves and seedpods have a peppery taste, and have been reported to have enormous biological activities
(Sarikami & Yanmaz 2011). It is documented to possess, tocopherol, phenolic compounds, nitrogen compounds,
terpenoids, and some other endogenous metabolites, which are rich in antioxidant activity (Muanda et al. 2011).
Rashad seeds contain two sets of fat-soluble compounds, and represent the first group of tocopherols, which
consists of (21ppm) alpha-tocopherol and (1422 ppm) gamma-tocopherol and (35ppm) Sigma-tocopherol, The
second group are tocotrienols (Moser et al. 2009). Many natural dietary agents, including vegetables, fruits,
herbs, and spices have been used in traditional medicines, as non-conventional treatments, for thousands of years,
but without sufficient scientific proofs. If effective, natural agents might lead to the development of natural and
novel drugs with low or no side effects (Mahassni & Al-Reemi 2013). Although vitamin E has been known as an
essential nutrient for reproduction since 1922, we far from understand the mechanisms of its physiological
functions (Brigelius-Flohe & Traber 1999). Vitamin E cannot be synthesized by humans and must be obtained
from the diet with an abundant source found in vegetable oil, nuts, and egg yolks (Ni & Yeh 2007). To date,
there are approximately 100 publications on this topic, which highlight the beneficial effects of this antioxidant
on viability, membrane integrity and motility of spermatozoa of different species.
2. Materials and Methods
2.1 Preparation of Extracts
Garden cress seeds (Lepidium sativum L.) were obtained from the local market in Hilla City, Iraq. The seeds
were cleaned and rendered free of dust, then stored in polyethylene bags in the refrigerator until used. For
tocopherol extraction, garden cress seeds were crushed, by using a household mill (Braun, Germany), and then 5
g sample was extracted with 25mL of (85% hexane : 15% ethyle acetate) for 24 hour by soxhlet method. The
extract was filtered out and evaporated to dryness by oven at 450
C for 24 hour (Harborn 1984).
2.2Experimental animals
Twenty (20) New Zealand White male rabbits aged 4 months and averagely weighing 1.513 gram and put in
cage under control of water, diet, light duration (12hour light-12hour dark). These animals were divided into 4
groups (5 animals for each group), control group was treated orally with corn oil and experimental groups were
treated orally with 32, 64, and 96 mg/kg b.w. of tocopherol extraction and for 50 days daily with these
concentration. The animals were seduced after a period end of experience with chloroform then the epididymis
caudal was excised to calculate the rate of sperm concentration in epididymis caudal, sperm motility percent, and
grade activity, and find MED50 for each parameter, and then calculating the average of the above doses affecting.
Effective dose of extract was used after its appointment in the first experiment to know its impact on
2. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.3, No.8, 2013
44
reproductive efficiency of rabbits, using 10 male rabbit, and these animals were divided into 2 groups, and by 5
animals per group and treated as described below:
• Group A: control group, treated orally with corn oil for 50 days.
• Group B: tocopherol group, treated orally with 33.6 mg/kg b.w. of extract for 50 days.
The animals have been weighed after the expiration of the effective dosage using the balance, and they were
seduced after a period end of experience with chloroform and all of the left testicle and left epididymis were used
to study of sperm parameters.
2.3 Statistical analysis
Results were presented as Mean ± S.E. Statistical analysis was done using SPSS and was conducted using one
way ANOVA to compare the means, F-test, T-test, correlation coefficient, and use the Least Significant
Difference (LSD) in the comparison between the results and also regression analysis and regression coefficient.
Different letters was considered significant (P<0.05) (Randolph & Ciminera 1985; AL-Rawi 2000).
3. Results
In Vivo Experiments, the treatment of animals with 32 and 64 and 96 mg/kg b.w. of tocopherol extraction led to
get a significant increase (P<0.05) in each of sperm concentration of epididymis caudal and sperm motility
percent for the treated groups, compared with the control group, with no significant difference (P>0.05) between
groups. It has been observed a significant increase (P<0.05) in sperm motility percent with progressive
movement of the epididymis caudal for the group treated with 32 mg/kg b.w., compared with the control group
and the groups treated with 64 and 96 mg/kg b.w., and insignificant differences (P>0.05) between the treatment
group at 64 and 96 mg/kg b.w. (Fig.1,2 and 3).
Figures 4, 5 and 6 show a positive linear relationship between escalating doses of tocopherols extract and sperm
concentration, sperm motility percent, and sperm motility percent with progressive movement of the epididymis
caudal where the correlation coefficient were r = 0.756, 0.559, and 0.293; and the regression equation linear
standard as follows: y =103.24+0.995 (x), y = 85.36 + 0.108 (x), y = 71.72 + 0.082 (x) of the above parameters,
respectively.
The results revealed a significant increase in the three parameters of the epididymis caudal, so the values of
MED50 were 26.39, 16.019, and 58.382 mg/kg b.w. of tocopherols extract of the three criteria respectively. The
average was calculated for these values in order to be used in a subsequent experiment. Accordingly, the value is
equal to 33.6 mg/kg b.w. of tocopherols extract as shown in the table (1).
4. Discussion
The results showed that the use of tocopherol extract of Rashad seeds led to a significant increase in the rate of
sperm concentration in epididymis caudal. Because the tocopherol play a vital role for the activity of the body
(Jaiswal et al. 2004), and its fat-soluble antioxidant and kept the body from various chemical reactions of free
radicals, which poison the cells (Braun & Cohen 2007). The results of the present research agree with the
findings of the Frank et al. (2003) in their study on male rats treated with roots extract of Lepidium
meyenii plant, which led to improved sexual function, and activate the process of sperm formation in Mitotic
stages. And increase sperm production by 85% and sperm motility by 5% in humans (Chung et al. 2005). The
researcher (Mahaneem et al. 2011) has pointed out that the increase in the sperm concentration of rats treated
orally at 1.2 g/kg b.w. of honey for 28 days, returning to the presence of some antioxidants, including phenols,
flavonoids, vitamin A, E and catalase enzyme. Our results showed that the dosage of white male rabbits with
influential concentration of tocopherol extract; led to a significant increase in sperm motility percent, grade
activity, and sperm viability percent of epididymis caudal. The results of the research agreed with the results of
(Gonzalez et al. 2001) at 9 men dosage with 1500 and 3000 mg/kg/day from Lepidium meyenii roots for 4
months, since it was found increase in the sperm concentration and its activity. Several researchers noted that
Black Maca of Lepidium meyenii, more efficient in increasing of sperm concentration and sperm motility percent,
compared to the rest of other types (red root and yellow root) in male rats after 42 days of treatment, possibly
due to the influence of the Black Maca along the eighth stage of seminiferous epithelial tubules (Gonzales et al.
2006; Yucra et al. 2006). The decrease in abnormal sperm morphology percent of rabbits epididymus caudal
treated with effect dose of tocopherol extract in the current study, was similar to another study (Christie 2010),
which indicated that tocopherol, may protect sperm through its function as antioxidant of body tissues, by a
network of cellular anti-oxidation defenses, where inhibits alpha-tocopherol enzyme protein Kinase, and thus
reduces the reactive oxygen species. The researcher (Audet et al. 2004) showed that feeding piglets with
vitamins, including vitamin E led to an increase in testosterone concentration in the blood plasma of animals
treated after puberty, sperm production, and sperm motility percent and no change in abnormal sperm
morphology percent compared to the control groups. Vitamin E acts with other antioxidants, including vitamin C,
glutathione, selenium, and beta-carotene; in prevent lipid peroxidation, and reduce DNA damage (Meletis et al.
3. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.3, No.8, 2013
45
2006; Brink 2010). The tocopherol working in scavenging free radicals, and it concludes the body from free
radicals, which cause DNA damage (Naseem et al. 2007; Diggs 2009). The DNA damage by free radicals caused
to descendant distortions due to contain sperm membranes a high concentration of unsaturated fatty acids, which
are more susceptible to damage with types Reactive Oxygen Species (Sancheza et al. 2009). Vitamin E
supplementation has become a common procedure to promote growth and health and improve the qualitative
characteristics of farm animals, it has been demonstrated to be an efficient strategy for improving their
reproductive function. Germ cells are particularly vulnerable to oxidative damage and may require additional
antioxidant protection (Castellini et al. 2007). Also vitamin E is quite an effective antioxidant which protects
rabbit testis against lipid peroxidation (Aydilek et al. 2004).
5. Conclusion
Under the light of this research, it is concluded that tocopherol extraction of Lepidium sativum seeds is the most
important fat-soluble antioxidant .Our results revealed a significant increase in testicular sperm concentration,
epididymus sperm concentration and in the sperm count per gm of the testis, sperm motility percent, grade
activity, sperm viability percent, and decreased in the abnormal sperm morphology percent of epididymus caudal.
This suggests that tocopherol extraction of garden cress seeds (Lepidium sativum L.) could be used to enhance
human fertility.
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EpididymisCaudal(Million/Ml)
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act of Black Maca (Lepidium Meyenii) on Testicular Function in Adult Male Rats
Doses of Tocopherol Extract in the Rate of Sperm
of Male Rabbits
control 32 64 96
±0.49
±2.209±1.734
Concentration of Tocopherol Extraction
Mg/Kg
±3.8
b
a
b b
www.iiste.org
Saudi Journal of Biological Sciences 15:1-9.
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”, Industrial Crops and
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s Pharmaceutical Sciences”, 17th
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Effects of Cultivar and Developmental Stage on Glucosinolates in Garden
Effect of Different Fractions from
) on Testicular Function in Adult Male Rats”, Fertility
Sperm Concentration in the
5. Journal of Biology, Agriculture and Healthcare
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.3, No.8, 2013
Figure 2. Impact of Escalating Doses
Figure 3. Impact of Escalating Doses
Movement in the
Figure 4. Variation of Sperm Concentration
0
20
40
60
80
100
control
SpermMotilityPercentofEpididymis
Caudal
0
20
40
60
80
100
SpermMotilityPercentwithProgressive
MovementoftheEpididymisCaudal
0
20
40
60
80
100
120
140
160
180
200
0
EpididymisCaudalSperm
Concentration(Million/Ml)
a
Journal of Biology, Agriculture and Healthcare
093X (Online)
47
Doses of Tocopherol Extract in the Sperm Motility Percent
Caudal (Million/Ml) of Male Rabbits
Doses of Tocopherol Extract in the Sperm Motility Percent
in the Epididymis Caudal (Million/Ml) of Male Rabbits
Concentration Rate with Escalating Doses of Tocopherol Extract
control 32 64 96
±1.345
±1.539
±1.931 ±1.798
Tocopherol Extraction Concentration
Mg/Kg
control 32 64 96
±0.632
±2.105
±0.456 ±0.748
Tocopherol Extraction Concentration
Mg/Kg
32 64 96
Tocopherol Extraction Concentration
Mg/Kg
b b b
a
b
c c
www.iiste.org
Sperm Motility Percent in the Epididymis
lity Percent with Progressive
of Male Rabbits
Extract in Male Rabbits
6. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.3, No.8, 2013
48
Figure 5. Variation of Sperm Motility Percent with Escalating Doses of Tocopherol Extract in Male Rabbits
Figure 6. Variation of Sperm Motility Percent of Progressive Movement with Escalating Doses of Tocopherols
Extract in Male Rabbits
Table 1. Median Effective Dose 33.6 Mg/Kg B.W. of Tocopherol Extract in Sperm Parameters of the Testis and
Epididymis Caudal of White Male Rabbits after Oral Dosing for 50 Days
Groups
Sperm Parameters
Control Effect Dose 33.6Mg/Kg/Day of
Tocopherol Extraction
Testicular Sperm Concentration (Million/Ml) a 32.80 ± 0.769 b 81.00 ± 2.173
Epididymis Caudal Sperm Concentration (Million/Ml) a 67.80 ± 0.769 b 116.00 ± 2.173
Sperm Count Rate Per Gm of the Testis a 13.94 ± 0.407 b 30.48 ± 0.703
Sperm Motility Percent of Epididymis Caudal a 76.40 ± 1.757 b 95.60 ± 1.117
Sperm Motility Percent with Progressive Movement (a+b)
of the Epididymis Caudal
a 60.00 ± 0.633 b 72.40 ± 1.152
Sperm Motility Percent with Progressive Movement (c) of
the Epididymis Caudal
a 16.00 ± 0.632 b 23.00 ± 1.233
Sperm Motility Percent with Progressive Movement (d) of
the Epididymis Caudal
a 22.40 ± 0.607 b 4.80 ± 0.335
Abnormal Sperm Morphology Percent of Epididymis
Caudal
a 27.40 ± 0.727 b 20.80 ± 1.730
Sperm Viability Percent of Epididymis Caudal a 76.40 ± 2.273 b 85.00 ± 1.233
0
20
40
60
80
100
120
0 32 64 96
SpermMotilityPercent
Tocopherol Extraction Concentration Mg/Kg
0
20
40
60
80
100
0 32 64 96
SpermMotilityPercentofProgressive
Movement
Tocopherol Extraction Concentration Mg/Kg
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