EFFECT OF SYNBIOTIC ON GROWTH PERFORMANCE AND GUT MORPHOLOGY OF BROILER CHICKEN

1. EFFECT OF SYNBIOTIC ON GROWTH
PERFORMANCE AND GUT MORPHOLOGY
OF BROILER CHICKEN
WAQAR UL HASSAN
2011-ag-2155
M.Sc(Hons) Poultry Science

2. CONTENTS
 Introduction
 Review of Literature
 Conclusion
UNIVERSITY OF AGRICULTURE FAISALABAD SUB-CAMPUS TOBA TEK SINGH 12/20/2018 2

3. SYNBIOTIC
UNIVERSITY OF AGRICULTURE FAISALABAD SUB-CAMPUS TOBA TEK SINGH
 Food ingredient or dietary supplements combining probiotics and prebiotics in a form of
synergism, hence synbiotic
Pro-biotics Pre-biotics
Synbiotic
12/20/2018 3(Pandey et al.,
2015)

4. PRO-BIOTICS
 “Micro-organisms which when consumed in adequate amounts, confer a beneficial effect on
the host“
 Lactobacillus species, Bifidobacterium species and Saccharomyces cerevisiae (Baker’s yeast)
 Improves gut health
 Releases bacteriocins
 Decreases pH
 Stimulates immunity in the host
UNIVERSITY OF AGRICULTURE FAISALABAD SUB-CAMPUS TOBA TEK SINGH 12/20/2018 4
(Lee and Lillehoj, 2017; Mizock, 2015; Kabir, 2009 & Macfarlane et al., 2008)

5. PRE-BIOTICS
 “A substrate that is selectively utilized by host micro-organisms conferring a health benefit"
 Includes mannan-oligosaccharides (MOS), fructo-oligosaccharides (FOS), xylo-
oligosaccharides (XOS) and inulin
 Potentially promote the host health by interacting with beneficial bacterial growth as well as
preventing enteric infection
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(Macfarlane et al., 2008; Sugiharto, 2016 & Gibson et al., 2017)

6. SYNBIOTIC
 Synbiotic refers to nutritional supplements combining Pro-biotic and Pre-biotic that are
thought to act together; i.e. synergism
 True probiotic, without its prebiotic food, does not survive well in the digestive system
 Combination of a pro-biotic and a pre-biotic, i.e. Synbiotics, might be more effect than
either a pro-biotic or pre-biotic alone
UNIVERSITY OF AGRICULTURE FAISALABAD SUB-CAMPUS TOBA TEK SINGH 12/20/2018 6
(Panesar et al., 2009; Ashraf et al., 2013 & Abdel-Hafeez, 2017)

7. Benefits of Synbiotic
 Improves growth
 Stimulates intestinal architecture
 Improves nutrient absorption
 Competes against the pathogenic microbes
(Hamasalim, 2016)
UNIVERSITY OF AGRICULTURE FAISALABAD SUB-CAMPUS TOBA TEK SINGH 12/20/2018 7

8. Combinations for Synbiotic
 Bifidobacterium pseudocatenulatum and Inulin
 Lactococcus lactis and Inulin
 Bacillus subtilis, B. licheniformis and xylooligosaccharide
 Enterococcus faecium and fructo-oligosaccharides
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(Othaman, 2009; Madej J. P. and M. Bednarczyk, 2016; Chen et al., 2017 & Abdel-Wareth et al., 2018)

9. Effect of Synbiotic on Growth Performance of Broilers
(42 Days)
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Parameters Control Synbiotic
Body weight (g) 2058.10b 2299.19a
FCR 2.04a
1.88b
(Abdel-Raheem et al., 2012)
a-bMeans in the same row with different superscripts differ significantly (P <
0.05)
Diet + 0.2% pre-biotics and 0.3% pro-biotics in feed 12/20/2018 9

10. Influences of Different Growth Promoters on Duodenum
Histomorphometery and Weight Gain (42 Days)
Control Probiotic Prebiotic Synbiotic
Villus height (µm) 1636.52b 1610.90b 1693.62ab 1732.60a
Crypt depth (µm) 186.3a 185.04a 182.6ab 177.5b
Villus height: Crypt depth 8.78bc 8.68a 9.27ab 9.76c
Body Weight (g) 2011.26c 2075.57bc 2097.91ab 2153.78a
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abcMeans in the same row with different superscripts differ significantly (P <
0.05)
(Dizaji et al., 2013)

11. In-Ovo Injection of Synbiotics and Their Effect on Growth
Performance (Day 34)
Parameter Control Synbiotic
Body weight gain (kg) 2.064 ± 0.213b 2.120 ± 0.240a
FCR 1.569 ± 0.033a 1.566 ± 0.048b
12/20/2018UNIVERSITY OF AGRICULTURE FAISALABAD SUB-CAMPUS TOBA TEK SINGH 11(Oszmalek et al., 2015)
a-bMeans in the same row with different superscripts differ significantly (P < 0.05)
Saline and dissolved in saline compounds were injected in ovo on 12th day incubation

12. Effect of Dietary Synbiotic Supplement on Growth
Performance of Broiler Chickens Reared Under Heat Stress
Control Synbiotic (0.5 g/kg) Synbiotic (1 g /kg)
BW gain (g) 1076.77b ± 21.60 1099.13a,b ± 21.60 1162.77a ± 21.60
Feed intake (g) 2144.83b ± 27.73 2202.19b ± 27.73 2323.61a ± 27.73
Feed conversion
ratio (g/g)
1.99 ± 0.08 1.88 ± 0.08 1.99 ± 0.08
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a,bMean ± SE with different superscripts in the same row differ significantly (P < 0.05). Heat stimulation
began on day 15 at 32◦C for 9 h daily until day 42. (Mohammed et al., 2018)

13. Effect of Probiotics and Synbiotic on Ileum Villus Length and
Crypt Depth of 42 Day
Villus length (µm) Crypt depth (µm)
Control 458.3 ± 37.45 c 225.0±25.00
2.5 g/kg probiotic 675.0 ± 25.00 b 204.2±13.56
5 g/kg probiotic 637.5 ± 42.69 b 209.2±16.35
2.5 g/kg synbiotic 883.3 ± 33.33 a 225.0±17.07
5 g/kg synbiotic 666.7 ± 94.57 b 216.7±16.66
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abcMean values on the same column not sharing a superscript are significantly different
(p<0.05) (Beski and Al-Sardary, 2015)

14. Effects of Dietary Inclusion of Synbiotic on Intestinal
Morphological Parameters of Broilers.
Control Synbiotic
1. Duodenum
Villus height (µm 1640b 1647a
Crypt depth (µm) 149a 149b
Villus height/crypt depth 11.45b 12a
2. Ileum
Villus height (µm 614b 774a
Crypt depth (µm) 128a 117b
Villus height/crypt depth 4.86b 7.13a
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(Awad et al., 2008)
Within the same row means with different superscripts a-b are significantly different
P<0.05

15. Effect of Feed Additive Supplementations on
Histomorphological Parameters of the Ileum in Broilers
Control Synbiotic (1kg/ton)
Villus height (µm) 523.00 ± 27.17b 724.00 ± 8.87a
Crypt depth (µm) 131.00 ± 4.32a 134.75 ± 8.10b
Villus height: crypt depth 3.99 ± 0.1b 5.39 ± 0.29a
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a-b Means within a column with differing superscripts are significantly different at P < 0.01.
(Ghari et al., 2013)

16. Effect of Synbiotic on Morphologic Parameters of Duodenal
Villi in the Broiler Chickens At 42 Days of Age
Control Synbiotic 0.1% Synbiotic 0.2%
Villus height (mm)
0.26a 0.35b 0.36b
Villus width (mm)
0.27b 0.36a 0.28b
Surface area (mm2)
0.21b 0.42a 0.32ab
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a,b Means with different superscripts within the same row differ (P<0.05). (Hassanpour et al., 2013)

17. CONCLUSION
 SYNBIOTICS ARE PROVED TO BE A BENEFICIAL FEED ADDITIVES THAT INCREASE
GROWTH PERFORMANCE AND IMPROVE GUT MORPHOLOGY
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18. Thanks ?
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