DNA replication is the process by which DNA copies itself exactly. It occurs in the nucleus and any mistakes can lead to mutations. During replication, one strand of DNA acts as a template for the other new strand. The key steps are that the DNA double helix unwinds and separates, enzymes bring in complementary nucleotide bases, and the new bases are inserted to form two new DNA molecules, each with one original strand and one newly synthesized strand. This is known as semi-conservative replication.
DNA replication is a semiconservative process. It means that each strand acts as a template for the synthesis of a new complementary strand. Therefore, this process takes us from one parent molecule to two daughter molecules, with each newly formed double helix containing one new and one old strand.
DNA replication is a semiconservative process. It means that each strand acts as a template for the synthesis of a new complementary strand. Therefore, this process takes us from one parent molecule to two daughter molecules, with each newly formed double helix containing one new and one old strand.
SOS repair
a system that repairs severely damaged bases in DNA by base excision and replacement, even if there is no template to guide base selection. This process is a last resort for repair and is often the cause of mutations.
Reverse transcription of RNA, which refers to the conversion of the RNA template into its complimentary DNA strand (cDNA) is an essential step in the analysis of gene transcripts.
cDNA can be sequenced, cloned and applied to estimate the copy number of specific genes in order to characterize and to validate gene expression.
DNA Replication process of copyingdna genomerimalyan
DNA replication is the process by which the genome's DNA is copied in cells. Before a cell divides, it must first copy (or replicate) its entire genome so that each resulting daughter cell ends up with its own complete genome.
SOS repair
a system that repairs severely damaged bases in DNA by base excision and replacement, even if there is no template to guide base selection. This process is a last resort for repair and is often the cause of mutations.
Reverse transcription of RNA, which refers to the conversion of the RNA template into its complimentary DNA strand (cDNA) is an essential step in the analysis of gene transcripts.
cDNA can be sequenced, cloned and applied to estimate the copy number of specific genes in order to characterize and to validate gene expression.
DNA Replication process of copyingdna genomerimalyan
DNA replication is the process by which the genome's DNA is copied in cells. Before a cell divides, it must first copy (or replicate) its entire genome so that each resulting daughter cell ends up with its own complete genome.
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Diogo Sousa, Engineering Manager @ Canonical
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2. DNA ReplicationDNA Replication
Replication = DNA copies itselfReplication = DNA copies itself exactlyexactly
(Occurs within the nucleus)(Occurs within the nucleus)
Any mistake in copying =Any mistake in copying = mutationmutation
DNA mutation = chromosomal mutationDNA mutation = chromosomal mutation
3. A. Basic Facts of DNAA. Basic Facts of DNA
ReplicationReplication
1.1. ComplementaryComplementary
base pairingbase pairing
makesmakes
replication possiblereplication possible
CC -- GG
AA -- TT
4. A. Basic Facts of DNAA. Basic Facts of DNA
ReplicationReplication
2. One side of DNA2. One side of DNA
molecule ismolecule is
aa
templatetemplate
forfor
makingmaking
thethe
other sideother side
(strand)(strand) ?’s 1-3
5. B. Process of DNA ReplicationB. Process of DNA Replication
1.1. Uncoil & unzip DNA moleculeUncoil & unzip DNA molecule
Enzyme (-ase)Enzyme (-ase) breaksbreaks
weakweak
Hydrogen BondHydrogen Bond
between basesbetween bases
6. B. Process of DNA ReplicationB. Process of DNA Replication
2.2. Enzyme brings in complementary N-basesEnzyme brings in complementary N-bases
DNA Replicase
7. B. Process of DNA ReplicationB. Process of DNA Replication
3.3. Insert N-basesInsert N-bases
DNA Replication TutorialDNA Replication Tutorial
(Go here for on-your-own learning/review)(Go here for on-your-own learning/review)
trc.ucdavis.edu/.../week5/06dnareplication.html