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AP Biology 2007-2008
DNA Replication
Ch.12.2
AP Biology
DNA Replication
 Purpose: cells need to make a copy of
DNA before dividing so each daughter
cell has a complete copy of genetic
information
 3 proposed Models of Replication
AP Biology
Meselson and Stahl Experiment
AP Biology
Semi-Conservative Model
 Replication of DNA
 base pairing allows
each strand to serve
as a template for a
new strand
 new strand is 1/2
parent template &
1/2 new DNA
AP Biology
Anti-parallel strands
 Nucleotides in DNA
backbone are bonded from
phosphate to sugar
between 3 & 5 carbons
 DNA molecule has
“direction”
 complementary strand runs
in opposite direction
THIS WILL CAUSE A
PROBLEM FOR
REPLICATION
3
5
5
3
AP Biology
Bonding in DNA
….strong or weak bonds?
How do the bonds fit the mechanism for copying DNA?
3
5 3
5
covalent
phosphodiester
bonds
hydrogen
bonds
AP Biology
DNA Replication
 Large team of enzymes coordinates replication
AP Biology
Replication: 1st step
 Unwind DNA
 helicase enzyme
 unwinds part of DNA helix
 stabilized by single-stranded binding proteins
 PREVENTS DNA MOLECULE FROM CLOSING!
 DNA gyrase
 Enzyme that prevents tangling upstream from the replication
fork
single-stranded binding proteins replication fork
helicase gyrase
AP Biology
Replication: 2nd step
 RNA Primase
 Adds small section of RNA (RNA primer) to the
3’ end of template DNA
 Why must this be done?
 DNA polymerase 3 (enzyme that builds new DNA
strand) can only add nucleotides to existing strands
of DNA
AP Biology
DNA
Polymerase III
Replication: 3rd step
 Build daughter DNA
strand
 add new
complementary bases
 With the help of the
enzyme DNA
polymerase III
AP Biology
Replication: 4th step
 Replacement of RNA primer by DNA
 Done by DNA polymerase I
AP Biology
Limits of DNA polymerase III
 can only build onto 3 end of
an existing DNA strand
Leading & Lagging strands
5
5
5
5
3
3
3
5
3
5
3 3
Leading strand
Lagging strand
ligase
Okazaki
Leading strand
 continuous synthesis
Lagging strand
 Okazaki fragments
 joined by ligase
 “spot welder” enzyme
DNA polymerase III


3
5
growing
replication fork
AP Biology
DNA polymerase III
RNA primer is added
 built by primase
 serves as starter sequence for DNA polymerase III
HOWEVER short segments called Okazaki fragments
are made because it can only go in a 5 3 direction
DNA replication on the lagging strand
5
5
5
3
3
3
5
3
5
3 5 3
growing
replication fork
primase
RNA
AP Biology
NEXT DNA polymerase I
 removes sections of RNA
primer and replaces with
DNA nucleotides
STRANDS ARE GLUED
TOGETHER BY DNA LIGASE
Replacing RNA primers with DNA
5
5
5
5
3
3
3
3
growing
replication fork
DNA polymerase I
RNA
ligase
AP Biology
Replication fork
3’
5’
3’
5’
5’
3’
3’ 5’
helicase
direction of replication
SSB = single-stranded binding proteins
primase
DNA
polymerase III
DNA
polymerase III
DNA
polymerase I
ligase
Okazaki
fragments
leading strand
lagging strand
SSB

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DNA Replication 2014 ppt.ppt

  • 1. AP Biology 2007-2008 DNA Replication Ch.12.2
  • 2. AP Biology DNA Replication  Purpose: cells need to make a copy of DNA before dividing so each daughter cell has a complete copy of genetic information  3 proposed Models of Replication
  • 3. AP Biology Meselson and Stahl Experiment
  • 4. AP Biology Semi-Conservative Model  Replication of DNA  base pairing allows each strand to serve as a template for a new strand  new strand is 1/2 parent template & 1/2 new DNA
  • 5. AP Biology Anti-parallel strands  Nucleotides in DNA backbone are bonded from phosphate to sugar between 3 & 5 carbons  DNA molecule has “direction”  complementary strand runs in opposite direction THIS WILL CAUSE A PROBLEM FOR REPLICATION 3 5 5 3
  • 6. AP Biology Bonding in DNA ….strong or weak bonds? How do the bonds fit the mechanism for copying DNA? 3 5 3 5 covalent phosphodiester bonds hydrogen bonds
  • 7. AP Biology DNA Replication  Large team of enzymes coordinates replication
  • 8. AP Biology Replication: 1st step  Unwind DNA  helicase enzyme  unwinds part of DNA helix  stabilized by single-stranded binding proteins  PREVENTS DNA MOLECULE FROM CLOSING!  DNA gyrase  Enzyme that prevents tangling upstream from the replication fork single-stranded binding proteins replication fork helicase gyrase
  • 9. AP Biology Replication: 2nd step  RNA Primase  Adds small section of RNA (RNA primer) to the 3’ end of template DNA  Why must this be done?  DNA polymerase 3 (enzyme that builds new DNA strand) can only add nucleotides to existing strands of DNA
  • 10. AP Biology DNA Polymerase III Replication: 3rd step  Build daughter DNA strand  add new complementary bases  With the help of the enzyme DNA polymerase III
  • 11. AP Biology Replication: 4th step  Replacement of RNA primer by DNA  Done by DNA polymerase I
  • 12. AP Biology Limits of DNA polymerase III  can only build onto 3 end of an existing DNA strand Leading & Lagging strands 5 5 5 5 3 3 3 5 3 5 3 3 Leading strand Lagging strand ligase Okazaki Leading strand  continuous synthesis Lagging strand  Okazaki fragments  joined by ligase  “spot welder” enzyme DNA polymerase III   3 5 growing replication fork
  • 13. AP Biology DNA polymerase III RNA primer is added  built by primase  serves as starter sequence for DNA polymerase III HOWEVER short segments called Okazaki fragments are made because it can only go in a 5 3 direction DNA replication on the lagging strand 5 5 5 3 3 3 5 3 5 3 5 3 growing replication fork primase RNA
  • 14. AP Biology NEXT DNA polymerase I  removes sections of RNA primer and replaces with DNA nucleotides STRANDS ARE GLUED TOGETHER BY DNA LIGASE Replacing RNA primers with DNA 5 5 5 5 3 3 3 3 growing replication fork DNA polymerase I RNA ligase
  • 15. AP Biology Replication fork 3’ 5’ 3’ 5’ 5’ 3’ 3’ 5’ helicase direction of replication SSB = single-stranded binding proteins primase DNA polymerase III DNA polymerase III DNA polymerase I ligase Okazaki fragments leading strand lagging strand SSB