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Zakary Kadish
   Uses small pieces of DNA to identify:
       Criminals
       Parental Identity
       Bodies


   Most commonly used for court cases where
    DNA is used as evidence
   DNA is unique to its person-much like
    fingerprints

   Chances that you will find two people with the
    same DNA who are not identical twins is
    VERY low

   DNA is extremely reliable in identifying who a
    person is, much like a fingerprint can identify
    who someone is
       DNA is 99.9% similar between humans!
    There are 6 steps to fingerprinting:
    1.   Isolation of DNA
    2.   Cutting, sizing, and sorting of DNA
    3.   Transfer of DNA to nylon
    4.   Probing
    5.   Probing
    6.   DNA Fingerprint
**NOTE: THIS PROCESS WAS MADE TO DO AT HOME**
   Obtain a sample of DNA from mouth, and spit
    into a shot glass
   Add 3 pinches of table salt
   Add 1 mL. of dish soap-stir for 3 minutes
       Do not make bubbles
   Pour 5 mL. of alcohol down the glass’s side
   Wait for 5 minutes
       DNA will become visible, can be extracted with
        toothpick
   Restriction enzymes cut DNA
   DNA then sorted by size using electrophoresis
       DNA placed in gel, then electric current used to drag
        pieces through the gel
         Gel is made from seaweed agarose
   Gel used in electrophoresis is placed on nylon

   Allowed to sit and dry overnight, so that all
    liquid is evaporated, and only DNA is left
   Use restriction enzymes to cut DNA

   Add radioactive N-bases to DNA Pol, which
    are coded for a wanted gene

   Once the DNA Pol has synthesized a new DNA
    strand, the radioactive labels will be able to be
    read!
   The DNA fingerprint can now be assembled
       Have the radioactive labels transfer to the sheet
       Align the genetic information with that of the
        criminal/suspect


   You are now ready to catch a criminal!
   http://www.accessexcellence.org/RC/AB/BA/DNA_
    Fingerprinting_Basics.php
   http://vesolt.free.fr/dna.htm

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Dna fingerprinting science project pp

  • 2. Uses small pieces of DNA to identify:  Criminals  Parental Identity  Bodies  Most commonly used for court cases where DNA is used as evidence
  • 3. DNA is unique to its person-much like fingerprints  Chances that you will find two people with the same DNA who are not identical twins is VERY low  DNA is extremely reliable in identifying who a person is, much like a fingerprint can identify who someone is  DNA is 99.9% similar between humans!
  • 4. There are 6 steps to fingerprinting: 1. Isolation of DNA 2. Cutting, sizing, and sorting of DNA 3. Transfer of DNA to nylon 4. Probing 5. Probing 6. DNA Fingerprint
  • 5. **NOTE: THIS PROCESS WAS MADE TO DO AT HOME**  Obtain a sample of DNA from mouth, and spit into a shot glass  Add 3 pinches of table salt  Add 1 mL. of dish soap-stir for 3 minutes  Do not make bubbles  Pour 5 mL. of alcohol down the glass’s side  Wait for 5 minutes  DNA will become visible, can be extracted with toothpick
  • 6. Restriction enzymes cut DNA  DNA then sorted by size using electrophoresis  DNA placed in gel, then electric current used to drag pieces through the gel  Gel is made from seaweed agarose
  • 7. Gel used in electrophoresis is placed on nylon  Allowed to sit and dry overnight, so that all liquid is evaporated, and only DNA is left
  • 8. Use restriction enzymes to cut DNA  Add radioactive N-bases to DNA Pol, which are coded for a wanted gene  Once the DNA Pol has synthesized a new DNA strand, the radioactive labels will be able to be read!
  • 9. The DNA fingerprint can now be assembled  Have the radioactive labels transfer to the sheet  Align the genetic information with that of the criminal/suspect  You are now ready to catch a criminal!
  • 10. http://www.accessexcellence.org/RC/AB/BA/DNA_ Fingerprinting_Basics.php  http://vesolt.free.fr/dna.htm