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Datta PhD Defense 20 min presentation
The document describes a modular polymeric development platform for microfluidic applications. Key points: - The goal is to design, build, and test a general-purpose microfluidic development platform to enable transition from prototype to mass production. - The platform uses vertically stacked, modular microfluidic chips that can be easily assembled and interfaced with external components through fluidic and electrical interconnects. - Fabrication involves molding polymer chips using hot embossing, then post-processing like flycutting. Various applications are demonstrated including surface chemistry, optical waveguides, and protein crystallization. - The platform provides a standardized, low-cost and user-friendly system for developing and testing microfluid
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Datta PhD Defense 20 min presentation
- 1. Modular, Polymeric Development Platformfor Microfluidic Applications - Design, Fabrication,Testing and Examples Proyag Datta PhD Thesis Presentation
- 2. Microfluidics for Bio-MEMSApplications — MEMS sensors for analysis of biological elements — Applications ◦ Healthcare ◦ Defense ◦ Environment — Critical Aspects ◦ Low Cost – Disposable ◦ Polymers ◦ Multi-domain Technology – Bio-chemistry – Microfluidics – Electronics — Transition from prototype to Mass Production I-Stat®
- 3. ICs, Diode, Transistors TV, Radios Computers Prototype PCBs, Breadboard Mixers, Splitter, Pump, AgilentBio- Analyzer, I-Stat Components System Development Platform Micro-Electronics Microfluidics Analogy between Micro-Electronics and Microfluidics Missing Link : A development platform for Microfluidics
- 4. Outline of thisPresentation q Concept q Fabrication q Mold Insert q Hot Embossing q Post Processing q Applications q Surface Chemistry q Optical Waveguide q Protein Crystal Formation Thesis Goal: Design, build and test a general purpose microfluidic development platform
- 5. q Flexible q Compatibility q User Friendly q Modular q Rapid Fabrication q LowCost Development Platform Platform Concept – Specifications Input Sample Preparation Reaction/ Processing/ Separation Detection/ Analysis Output
- 6. Platform Concept § Individual functionalchips § Vertically assembly = Minimal dead volume § Passive Alignment of Chips § Electronics integration § Compatible w/ existing labware § Macro-Micro interconnections standardized Clamp Alignment Pins Electronic Connector Fluid Distribution Backplane Nozzles Syringe Ports Structural Block Modular Microfluidic Chip (MMC) Elements of Interconnect Block (ICB) Electronic Interconnect Macro-Micro Fluidic Ports Alignment Features Alignment Verification Window 75.5 mm 25.5 mm Region for Fluidic Layout
- 7. Hot Embossing Jenoptik HEX02Hot Embossing Machine at CAMD
- 8. Mold Molded Part LiGA Micromilling 50µm dia. Criteria • Cost • Turnaround Time • Surface Finish • Minimum feature • Geometry
- 9. Parameter Evaluation Curve Brassmold insert and molded chips on the bottom platen of molding machine T>>Tg T<<Tg T~Tg T>Tg 80 100 120 140 160 180 200 Temperature (°C) Displacement(mm) -0.500.51.01.52 -‐0.5 0 0.5 1 1.5 2 2.5 3 3.5 70 90 110 130 150 170 190 Displacement (mm) Temperature (°C) T~151°C T~162°C T~175°C Incomplete filling in corners Completely filled structure
- 10. Process Bias ofHot Embossed Parts Full factorial design of experiment (DOE) based study to evaluate dimensional variation as a function of process parameters. (32 molding runs, 4 data points per part = 128 Total data pts) Newton Thickness in mm Distance from Center (mm) Dimensionalchangeinmm Celsius Celsius Seconds Dimensionalchangeinmm
- 11. Post-Processing Cut Snap-off or dicinglocation Flycutting Tool Overall dimension of part independent of cutting operation Through-holes opened Thickness control to within 10µm Snap-off Boundary Structure Molded Chips before Processing Chips being flycut Chips after flycutting 500 µm dia through hole opened by flycutting
- 12. Passive Alignment -Concept Clamp with dowel pins Stack of chips V-grooves Acceptable Unacceptable Vias from one fluidic chip to next Alignment Fixture
- 13. Alignment Verification Window 75.5 mm 25.5mm Passive Alignment - Accuracy 0 0.02 0.04 0.06 0.08 0.1 0.12 0 5 10 15 20 Millimeters Assembly Attempt Alignment of 4 Chip Sets Overlaid Alignment Marks Nominal 100 µm Chip2 Chip1 35 40 45 50 55 60 65 70 1 2 3 4 5 AverageAlignmentAccuracy (microns) Square root of the number of contacts Chip Stack Alignment Marks 100µm gaps
- 14. Chip Sealing Cross Sectionof Sealed PMMA Chip (110 °C , 60 to 70 psi pressure, 1 hr) q Temporary Gasket (Open Access) q Permanent Seal q Sealing Methods q Adhesive q Laser q Ultrasonic q Thermal Sealing Standalone thermal sealing press 100 µm
- 15. Hardware Fluidic chip with electricallines and macro connector Microfluidic chips with the fluidic macro connector block Sealed fluidic chip Multimeter connected to the flat ribbon cable Flat ribbon cable to interconnect stack and measurement devices
- 16. Fluid In Fluid Out Nozzles to Interconnect Chip Fluid route from Syringe inlets to chip stack Bolt Holes Hardware Fluidic distribution manifold (backplane) Modular Microfluidic Chip Stack
- 17. Biochemical Protocol onSilicon Chips Chemilumenscent signal captured on X-ray film. Chips with 1:10 serial dilutions of protein conjugates, Strepavidin – Horse Radish Peroxidase(S-HRP) were placed in different chambers with the far right being the highest concentration of S-HRP. Bio-chemistry experiments on silicon surface in a microfluidic environment Open access to chips for Microspotting
- 18. Application - OpticalWaveguide q Waveguides used to excite fluorescent probes and detect the emitted light q Goal : deliver excitation light to an extended/wide region, such as a microfluidic channel, exciting all fluorescent probes therein Microscope objective Excitation Light Fluorescent Probe Filters CCD Camera Emitted Light To Computer Light in Light out Light leaking into the channel Working of the waveguide Light emitted from Fluorescent probe Floor of Microfluidic channel contains light by total internal reflection Microfluidic channel Light ‘leaking’ into microfluidic channel Fluorescent probe attached to DNA Air Air
- 19. Application - OpticalWaveguide Waveguide Fluidic ChannelTop mold insert Bottom mold insert Micro fluidic channel Polymer Air Fabrication of chip by double sided aligned molding Image of Fluorescence Excitation using embedded waveguide Cross- section of chip 0 4 8 12 16 20 1000 1250 1500 1750 2000 FluorescenceIntensity Distance from the beginning (mm) Fluidic Channel Laser Diode Microscopic objective Filters PMMA chip XYZ Translation Stages Optical fiber CCD Tube Coupling
- 20. Application – ProteinCrystallography Oil Precipitant Protein Electronic Wiring for feedback Interconnect Slide Removable Storage Slide Differentproportionsof macromoleculeandprecipitantmix Protein MoleculesPrecipitant Separating fluid (oil)
- 21. Real time X-raySpectroscopy of Nanoparticles Creation Chemistry Controlled Cell Growth Other Applications X-rays from Synchrotron Beamline Detector Reagents Product Microscope Alignment Optics
- 22. Summary — Microfluidic DevelopmentPlatform ◦ Vertically stacked ,Modular chips ◦ User friendly ◦ Compatible w/ Standard Labware — Fabrication Technologies ◦ Mold insert ◦ Hot embossing ◦ Post Processing — Applications ◦ Bio Protocol ◦ Waveguide ◦ Protein Crystallography
- 23. Outlook Integration of DiscreteComponents q Electrodes q Electromagnets q Piezo electric actuation q Reactor beds q Heaters / Coolers q CMOS & other silicon die Integrated System Development using the Microfluidic Development Platform Modular Microfluidic Development Platform Increase Functionality Specific Applications
- 24.