Culture collection , Purpose of culture collection center and some famous International Culture Collection Center and National Culture Collection Centers of India .
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
Bergey's Manual and it's classification. A brief concised presentation prepared for taking seminar and classes.
Volume II (Edition 2) described more in detail.
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
Bergey's Manual and it's classification. A brief concised presentation prepared for taking seminar and classes.
Volume II (Edition 2) described more in detail.
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
Introduction :
Mycorrhizae are mutualistic symbiotic associations formed between the roots of higher plants and fungi.
Fungal roots were discovered by the German botanist A B Frank in the last century (1855) in forest trees such as pine.
In nature approximately 90% of plants are infected with mycorrhizae. 83% Dicots,79% Monocots and 100% Gymnosperms.
Convert insoluble form of phosphorous in soil into soluble form.
Extremophilic organisms are organisms that can survive exremities that are detrimental for other forms of life. Here is a presentation that discuss such microorganisms in detail
General features of Proteobacteria, alpha Proteobacteria
subscribe youtube channel: Dharmesh Sherathia
https://www.youtube.com/watch?v=JxOIqxYmerk&t=348s
join me on insta @dharmesh.biology
Secondary screening of industrial important microbes DhruviSuvagiya
Detection and isolation of a microorganism from a natural environment like soil containing large number of microbial population is called as screening. It is very time consuming and expensive process.
Microbial interactions are ubiquitous, diverse, critically important in the function of any biological community.
The most common cooperative interactions seen in microbial systems are mutually beneficial. The interactions between the two populations are classified according to whether both populations and one of them benefit from the associations, or one or both populations are negatively affected.
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
Introduction :
Mycorrhizae are mutualistic symbiotic associations formed between the roots of higher plants and fungi.
Fungal roots were discovered by the German botanist A B Frank in the last century (1855) in forest trees such as pine.
In nature approximately 90% of plants are infected with mycorrhizae. 83% Dicots,79% Monocots and 100% Gymnosperms.
Convert insoluble form of phosphorous in soil into soluble form.
Extremophilic organisms are organisms that can survive exremities that are detrimental for other forms of life. Here is a presentation that discuss such microorganisms in detail
General features of Proteobacteria, alpha Proteobacteria
subscribe youtube channel: Dharmesh Sherathia
https://www.youtube.com/watch?v=JxOIqxYmerk&t=348s
join me on insta @dharmesh.biology
Secondary screening of industrial important microbes DhruviSuvagiya
Detection and isolation of a microorganism from a natural environment like soil containing large number of microbial population is called as screening. It is very time consuming and expensive process.
Microbial interactions are ubiquitous, diverse, critically important in the function of any biological community.
The most common cooperative interactions seen in microbial systems are mutually beneficial. The interactions between the two populations are classified according to whether both populations and one of them benefit from the associations, or one or both populations are negatively affected.
L'Institut Pasteur de Tunis et sa stratégie en biosécurité/biosûertéPasteur_Tunis
Présentation de Hechmi Louzir, directeur général de l'IPT durant le symposium organisé conjointement entre le Robert Koch Institute et l'Institut Pasteur de Tunis "Current challenges of Biological Risks and Heealth Security" (22-23 septembre 2016)
National Agricultural Innovation Project (NAIP), ICAR and the International Food Policy Research Institute (IFPRI) organized a two day workshop on ‘Impact of capacity building programs under NAIP’ on June 6-7, 2014 at AP Shinde Auditorium, NASC Complex, Pusa, New Delhi. The main purpose of the workshop was to present and discuss the findings of the impact evaluation study on capacity building programs under NAIP by IFPRI. The scientists from ICAR and agricultural universities were sent abroad to receive training in specialized research techniques. Post-training, scientists were expected to work on collaborative projects within the ICAR, which would further enrich their knowledge and skills, expand their research network and stimulate them’ to improve their productivity, creativity and quality of their research. The ICAR commissioned with IFPRI (International Food Policy Research Institute) to undertake an evaluation of these capacity building programs under NAIP in July 2012. The workshop shared the findings on the impact of capacity building programs under NAIP and evolve strategies for future capacity building programs
Dive in microbial biodiversity!
- Microbial resources success stories
- The potential of culture collections and their networks
- EMbaRC project and its outputs
- Microorganisms and the bioeconomy
- Future developments and plans
Status of ICT structure, infrastructure and applications existed to manage an...RABNENA Network
Status of ICT structure, infrastructure and applications existed to manage and disseminate information and knowledge of Agricultural Biotechnology Innovations Information in Saudi Arabia, Almotairy, Hany Mohammed S. Al-Assaf, King AbdulAziz City for Science and Technology (KACST)
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. • Culture collection are centers
that provide authentic
examples of organism that can
be grown or maintained in the
laboratory ; they normally
have a public service role and
provide associated information
and services
• Prof. Frantisek Karl was the
first one to collect the culture .
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
2
https://en.wikipedia.org/wiki/Karel_Franti%C5%A1ek_Koch
Karl Frantisek Koch
Born : 29 June 1890
Died : 24 January 1981
Nationality : Czech
3. 3
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
• Culture Collections aims at acquisition , preservation,
authentication , production preservation, cataloguing and
distribution of standard reference microorganism , cell
lines and other materials for research and repositories of
type strains.
4. • According to World directory of culture collections (Sixth Version
2014):
* Culture collection : 802
* Countries and region : 78
*Microorganism :3272863
* Bacteria :1429816
* Fungi : 872094
* Virus :39491
* Cell line : 33020
Aysha Riza & Rinu Rolly St Mary's College
Thrissur
4
5. • Collection and maintenance of important and useful organism
• Maintain culture in viable and contaminant free state.
• To make available at nominal cost to researcher ,workers,
teachers ,students or industries .
• Preparation of their information catalogue .
• Comparison of microorganisms
5
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
6. • Initiative of government
department of biotechnology.
• Housed at National Center of Cell
Science (NCCS), Pune
• Depository of microorganism (for
isolates generated from microbial
prospecting and screening
program initiated by DBT)
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
6
www.nccs.res.in
Microbial collection Center, Pune
7. • Status of collection – governmental
• Sponsor – Department of Biotechnology ,Ministry of Science
and Technology, Government of India
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
7
Services :
Storage (bacteria, fungi)
Distribution
Identification
8. Trainings :
Culture and preservation methods
Identification
8
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
NUMBER OF STRAINS IN MCC
Type Holding strains (Total
number)
Bacteria 149314
Fungi 15338
9. • Established in 1986 .
• MTCC roots at Institute of
Microbial Technology (IMTECH),
Chandigarh .
• Sponsor – Department of
biotechnology and Government
of India and Council of Scientific
and Industrial Research .
• Status – Semi governmental
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
9
https://www.imtech.res.in/focus-area/microbial-type-culture-
collection
Microbial Type Culture collection and Gene
bank , Chandigarh
10. Main objectives
Act as depository
To supply authentic microbial cultures
To provide related services to the scientist working in
research institutions universities and industries .
10
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
11. Services :
Distribution (bacteria ,fungi ,plasmids)
Identification
Patent deposits
Storage services
Training :
Culture and preservation methods
Genetic engineering
Identification
bacterial genetics
11
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
12. NUMBER OF STRAINS IN MTCC
Type Holding Strains (Total number)
Algae 1124
Bacteria 1245
Fungi 575
Yeasts 85
12
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
13. • Established in 1951 .
• One of the oldest biological resource center .
• Status – Governmental
• Housed at National Chemical Laboratory (CSIR) New Delhi
Services:
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
13
Patent deposits (Bacteria ,yeast , fungi)
Storage services (microbial strain)
Distribution (bacteria ,yeast ,fungi ,algae)
14. Trainings :
Management of culture collection
Culture and preservation method
Genetic engineering
Systematics.
Identification
14
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
NUMBER OF STRAINS IN NCIM
Type Holding Strains ( Total number)
Algae 20
Bacteria 1400
Fungi 950
Yeasts 600
15. • Established in 1962.
• Status of the collection – ICAR.
• Housed at National Dairy
Research Institute ,Karnal
Haryana .
• Sponsor – N.D.R.I, Karnal and
ICAR , New Delhi.
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
15
https://www.showincity.com/venues/v-18667/national-dairy-research-
institute
National Culture of Dairy Collection , Harayana
16. Services :
Distribution (bacteria, fungi, yeast, dairy starters ).
Training :
Culture and preservation method
Quality control
Use of computers in culture collections
Preservation , propagation and quality control of Dairy starter
cultures
Preparation of fermented milks
16
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
17. NUMBER OF STRAINS IN NCDC
Type Holding strains (Total number)
Bacteria 400
Fungi 15
Yeasts 20
Dairy starter cultures 25
17
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
18. • Established in 2008
• Initiative by Department of Science and Technology at Agharkar Research
Institute, Maharashtra.
• NFCCI aims at conserving fungi as resource pool for future research and
related projects.
• Housed at MACS’ Agharkar Research Institute , Maharashtra
• Status – Governmental ,National Facility
• Sponsor – Department of Science and Technology ,Government of India
and MACS’ Agharkar Research Institute , Pune , India .
18
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
19. Services :
Storage services (micro and macro fungi)
Distribution
Identification
NUMBER OF STRAINS IN NFCCI
Type Holding strains (Total number)
Fungi 3050
19
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
20. Training:
Management of a culture collection
Culture and preservation methods
Quality control
Use of computers in culture collection
Systematics
Identification
Molecular systematics
Fermentation and isolation of secondary products
20
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
21. • Established in 1925.
• Largest general culture collection in
the world.
• Serves as centre for acquisition ,
preservation, authentication and
distribution of microbial specimens
standard reference microorganism ,
cell lines and other materials for
research and development .
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
21
American Type Culture Collection ,USA
https://www.atcc.org/~/media/PDFs/webinars/Presentations/2017/M
ycoplasma%20Detection%20Webinar.ashx
22. • Status – Private non profit organization.
• Designated as first International Depository Authority (IDA)
(International Budapest Treaty for biotechnology patent
,1981)
Programs:
22
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
Collection , Research and service program .
Professional Service program .
Information Service program
23. Services:
Patent deposits .
Storage services (safe deposit).
Identification (authentication services).
23
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
24. Training:
Management of culture collection
Culture and preservation methods
Quality control and Genetic engineering
Karyotyping.
Use of computer in culture collections .
Cytogenetics.
Diagnostic virology
Fermentation
Hybridomas and monoclonal antibodies
Medicinal mycology
24
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
25. NUMBER OF STRAINS IN ATCC
Type Holding strains
(Total number)
Bacteriology:
Bacteria & bacteriophage
18000
Cell biology:
Cell lines & hybridomas
4300
Molecular biology:
Recombinant DNA Material
0
Mycology &botany :
Filamentous fungi & yeasts
46000
Plant tissue 79
Seeds 1000
Protists (including algae) 2000
Virology: Animal virus, chlamydiae , rickettsiae
& antisera
2200
Plant viruses & antisera 1500
25
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
26. • Established in 1981
• Housed and maintained by RIKEN Bio Resource and Research
Center at Tsukuba ,Ibaraki ,Japan
• Status – semi-governmental
• Sponsor – The Japanese government (Ministry of education ,
culture, sports , science and technology )
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
26
27. • This division collects , preserves and distributes cultured
microbial strain as one of the leading culture center in the
world
• Strains held at JCM are limited to those classified as Risk 1 and
Risk 2
• ISO Certified for its quality management system
27
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
28. Services :
Distribution
Training :
Culture and preservation methods
Systematics.
Identification
28
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
29. NUMBER OF STRAINS IN JCM
Type Holding strains (Total number)
Bacteria 18822
Yeasts 4208
Archaea 838
Microalgae 26
Filamentous fungi 3904
29
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
30. • Established in 1963.
• Rooted and maintained by Masaryk University
• Status of collection – university
Catalogue of cultures include :
Bacteria and Archea.
Filamentous fungi and yeast.
Bacteriophages.
Fungi ( deuteromycetes, ascomycetes, mucorales)
30
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
31. Services :
Patent deposits (bacteria , fungi , yeasts)
Storage service (bacteria , fungi)
Identification (bacteria [aerobic bacteria])
31
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
Training :
• Management of a culture collection
• Culture and preservation methods
• Identification
32. NUMBER OF STRAINS IN CCM
Type Holding strains(Total number)
Bacteria 2600
Fungi 750
Yeasts 22
32
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
33. • Established in 1920
• Serves to “ provide a trustworthy source of authentic bacteria for use in
scientific studies”.
• NCTC is one of the four culture collections of public health England
• Status – governmental
• Sponsor – department of health
33
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
34. • It is longest established collection of its type anywhere in the world
• It also serves as United Nations Educational , Scientific and Cultural
Organization(UNESCO) Microbial Resource Center (MIRCEN)
• NCTC holds nearly 6000strains most of which have caused infections in
humans or animals and most of them are medical , scientific and
veterinary importance world wide
• It also serves as an International Depository Authority (IDA) patent
depository for bacterial cultures.
34
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
35. Strains grouped by specific characteristics
Antibacterial susceptibility control and resistance reference strains
Control strains for the microbiological testing of water samples for
Pseudomonas aeruginosa.
Clostridium difficile strains – Ribotype and toxin status
NCTC reference strains for standards for microbiology investigations (UK-
SMI)
World Data Center for Microorganism (WDCM) reference strains
35
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
36. Services
Patent deposits (bacteria)
Storage service (bacteria)
Distribution (bacteria)
Identification (bacteria)
NUMBER OF STRAINS IN NCTC
Type Holding strains (Total number)
Bacteria 5000
Plasmids 600
36
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
37. • Established in 1979 as central
culture collection network of
various collection.
• Rooted at institution of
microbiology , China academy of
science
• Status – governmental
• Sponsor – Chinese academy of
sciences
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
37
http://www.cgmcc.net/
China General Microbial Culture Collection
center ,China
38. • ISO certified
• Focus – to preserve , supply and maintain living microbial resources .
Contribute to scientific community by providing high quality microbial
source to research fields.
• Maintain approximately 40000 by the end of 2013 including
bacteria,archea, fungi
• Strains held at CGMCC classified to risk 1 0r 2 group
• Information of strain available at online catalogue database of CGMCC
38
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
39. • 8700 strain for patent purpose
• To improve and expand collection continously CGMCC exploit new
resources from various environment , describing microbial taxa,
accept useful microbes researcher and applied sectors
• All strains are subjected to extensive quality control
39
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
40. Services :
• Patent deposit (Archea ,Bacteria, Yeasts, Fungi, Algae, Viruses, plasmids,
cell lines, Plant seed)
• Storage services (Archea ,Bacteria, Yeasts, Fungi)
• Distribution (Archea ,Bacteria, Yeasts, Fungi , cell lines)
• Identification (Archea ,Bacteria, Yeasts, Fungi )
NUMBER OF STRAINS IN CGMCC
Type Holding strains
Bacteria 19820
Fungi 16750
Yeast 5360
Cultures for patent purpose 11976
40
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
41. • Avinash Sharma and Yogesh Shouche. (2014). Microbial Culture Collection (MCC) and
International Depositary Authority (IDA) at National Centre for Cell Science , Pune . Indian
Journal of Microbiology .129-133.
• https://mtccindia.res.in/
• https://www.ncl-india.org/files/ncim/Default.aspx
• http://www.ndri.res.in/ncdc/Members/NCDC_Catalogue_2016.pdf
• http://nfcci.aripune.org/
• http://nfcci.aripune.org/about_us.php
• http://gcm.wfcc.info/
• http://www.wfcc.info/index.php/wdcmdb/
• http://www.wfcc.info/ccinfo/collection/by_country/u/
• http://www.wfcc.info/ccinfo/collection/by_country/j/
• http://www.wfcc.info/ccinfo/collection/by_country/c/
• http://www.wfcc.info/ccinfo/collection/by_country/i/
• http://www.nccs.res.in/mcc/index.html
• http://www.imtech.ernet.in/mtcc/
• http://www.ndri.res.in/
• http://http.atcc.org/
• http://jcm.brc.riken.jp/
• http://www.szu.cz/cnctc/
• www.phe-culturecollections.org.uk
• http://www.cgmcc.net
41
Aysha Riza & Rinu Rolly
St Mary's College Thrissur