The document discusses the use of the cre-lox system for targeted gene editing in mice, highlighting the generation of conditional alleles through breeding floxed mice with cre recombinase mice. It details the characterization and discovery of recombinase activities across various tissues and references a database for locating information about targeted alleles. For further inquiries, users are encouraged to contact MGI support.

1. Module 2.
cre-lox and cre
recombinases in MGI

2. The cre-Lox system
1. mouse with a targeted
“floxed” allele
2. mouse with a cre
recombinase enzyme
expressed under the
control of a transgenic
promoter
 Breed together to
generate a line that
carries both
 Tissue specific deletion of
floxed allele in tissues
where cre recombinase is
expressed

3. Targeted conditional and
conditional-ready alleles

4. Summary of Brca1 alleles: use
category to identify attributes

5. Locating information about a
targeted allele
Reference

6. cre recombinase transgenes:
by driver or by tissue

8. cre recombinases with activity
examined in bone marrow

10. Recombinase Activity table

11. Systems level recombinase
activity detail

12. Summary
MGI allows users to discover of conditionally
targeted alleles and appropriate
recombinase
Transgene recombinase alleles are
characterized across the whole mouse with
data presented from multiple sources
contact mgi-help@jax.org with any questions