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OBT554
Unit 3
Dr K.Geetha
Associate Professor, Dept of
Biotechnology, Kamaraj College of Engg
& Tech, Madurai
 The influence of food water content on
perishability has been known since ancient
times.
 Between 15,000 and 10,000 BC our ancestors
began to preserve excess fish, meat and fruit
by drying in the wind and sun.
*The unbound or free water
molecules in the food is
referred to water activity.
*This unbound water
molecules aid in the
growth of microbes-
bacteria, fungi etc..
*It’s value ranges from 0 to
1.
The food get spoiled on
microbial growth over the
fruits and vegetables having
water activity greater than
0.95.
The moisture condition of a product can be measured as the equilibrium
relative humidity (ERH) expressed in percentage or as the water
activity expressed as a decimal.
*
*
 aw is equal to f/fo, where f is the fugacity of
water in the system (i.e., the escaping
tendency of water from solution) and fo is
the fugacity of pure water at the same
temperature.
 Water activity depends on water
concentration as shown by equation:
 Where is the mole fraction and is the
activity coefficient of the water
 The optimum aw for growth of the majority of
microorganisms is in the range 0.99±0.98.
 Every microorganism has limiting aw values below which it
will not grow, form spores, or produce toxic metabolites.
 Minimum aw for growth is always equal or lower than
minimum aw for toxin production.
 Water activity reduction through curing, conserving,
drying, evaporation is one important method for food
preservation.
 Vapour pressure manometers
 Dew point hygrometer
 Freezing point depression methods
 Electric hygrometers
 Fibre hygrometer
 aw can be influenced in at least three ways
during the preparation of dried, intermediate
and high moisture foods:
1. Water can be removed by a dehydration,
evaporation or concentration process.
2. Additional solute can be added.
3. Combining 1. and 2. when the food pieces are
infused with the solutes and additives and then
partially dried.
 At various stages of the food distribution
chain, it is used during storage, processing,
and/or packaging.
 Used for obtaining long shelf-life products.
 Used to obtain high moisture novel foods
The spoilage due to water activity can be
controlled by,
*Drying
*canning
*Freezing
The canned food has good shelf
life time.
 The free water molecules in the fruits are
removed.
 Suitable for juicy fruits like grapes.
 Grapes are dried to get raisin.
 The food is cooled to temperature at which
microbial growth is deterioted.
 Unbound water molecules get inactive.
 Example : freezing of sea food to avoid
spoilage during transportation.
Control of water activity
Control of water activity

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Control of water activity

  • 1. OBT554 Unit 3 Dr K.Geetha Associate Professor, Dept of Biotechnology, Kamaraj College of Engg & Tech, Madurai
  • 2.
  • 3.
  • 4.  The influence of food water content on perishability has been known since ancient times.  Between 15,000 and 10,000 BC our ancestors began to preserve excess fish, meat and fruit by drying in the wind and sun.
  • 5. *The unbound or free water molecules in the food is referred to water activity. *This unbound water molecules aid in the growth of microbes- bacteria, fungi etc.. *It’s value ranges from 0 to 1.
  • 6.
  • 7. The food get spoiled on microbial growth over the fruits and vegetables having water activity greater than 0.95.
  • 8. The moisture condition of a product can be measured as the equilibrium relative humidity (ERH) expressed in percentage or as the water activity expressed as a decimal. * *
  • 9.  aw is equal to f/fo, where f is the fugacity of water in the system (i.e., the escaping tendency of water from solution) and fo is the fugacity of pure water at the same temperature.  Water activity depends on water concentration as shown by equation:  Where is the mole fraction and is the activity coefficient of the water
  • 10.
  • 11.  The optimum aw for growth of the majority of microorganisms is in the range 0.99±0.98.  Every microorganism has limiting aw values below which it will not grow, form spores, or produce toxic metabolites.  Minimum aw for growth is always equal or lower than minimum aw for toxin production.  Water activity reduction through curing, conserving, drying, evaporation is one important method for food preservation.
  • 12.
  • 13.  Vapour pressure manometers  Dew point hygrometer  Freezing point depression methods  Electric hygrometers  Fibre hygrometer
  • 14.
  • 15.  aw can be influenced in at least three ways during the preparation of dried, intermediate and high moisture foods: 1. Water can be removed by a dehydration, evaporation or concentration process. 2. Additional solute can be added. 3. Combining 1. and 2. when the food pieces are infused with the solutes and additives and then partially dried.
  • 16.
  • 17.  At various stages of the food distribution chain, it is used during storage, processing, and/or packaging.  Used for obtaining long shelf-life products.  Used to obtain high moisture novel foods
  • 18. The spoilage due to water activity can be controlled by, *Drying *canning *Freezing
  • 19.
  • 20. The canned food has good shelf life time.
  • 21.
  • 22.  The free water molecules in the fruits are removed.  Suitable for juicy fruits like grapes.  Grapes are dried to get raisin.
  • 23.
  • 24.  The food is cooled to temperature at which microbial growth is deterioted.  Unbound water molecules get inactive.  Example : freezing of sea food to avoid spoilage during transportation.