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Presented by-
Akanksha Shukla
Dept. Of Plant Pathology
Conidial Ontogeny
Contents
 Introduction
 Saccardoan spore types
 Conidial Ontogeny
 Types of Conidium development
 Hughes’s Classification
 Conclusion
Introduction
Deuteromycetes fungi are the septate, mycelial fungi which reproduce
exclusively by asexual means and are known as imperfect fungi or
anamorphic fungi. They lack sexual stage (telomorph) and are believed to
represent the conidial stage of higher fungi. The asexual reproduction is
through conidia (mitospore). There is a tremendous variety of
morphologically different conidia produced. The first attempt to classify
these conidial fungi was of Saccardo’s. The study of development of
conidia based on its origin is referred to as “Conidial Ontogeny”. The two
principle types of conidum ontogeny are ‘Thallic’ and ‘Blastic’. Hughes
proposed eight different types of conidium development based on its
origin.
Saccardoan Spore Types
P.A. Saccardo (1845-1920)
 “Sylloge Fungorum” (1882-1972)
 Developed first system of classifying Hyphomycetes fungi based on
morphology of spores:-
Color
 Hyaline or bright (hyalo-)
 Pigmented (phaeo-)
Shape and Septation
1. Amerosporae:
Conidia non septate, spherical, ovoid to elongated.
2. Didymosporae:
conidia two-celled, ovoid to oblong.
3. Phragmosporae:
conidia 3 or more celled by
transverse septa.
4. Dictyosporae:
conidia divided by both transverse
and longitudinal septa(muriform).
5. Scolecosporae: conidia
slender, filiform, one to several
celled; hyaline or coloured.
6. Helicosporae
(Allantosporae): Conidia
cylindrical, curved (allantoid);
hyaline or coloured.
7. Staurosporae: Conidia stellate
(star-shaped), radiate or trifurate,
one to several celled; hyaline or
coloured.
Conidial Ontogeny
 Conidia/Conidiospores -(sing. conidium) Conidia may be defined as
asexual, non-motile spore that belong to the anamorphic stage of a fungus
life cycle.
The word is derived from the Greek konis ‘dust’ + the diminutive
suffix -idium (Sutton, 1986).
 Conidiophore -Specialized hyphae that bears one or more conodiogeneous
cells.
 Conidiogeneous cell - The hyphal cell from which a conidium is formed
directly.
 Conidiogenesis - The process of formation of conidium.
 Conidia may originate from conidiogeneous cells by either of the two
ways:-
1. Thallic
2. Blastic
Thallic Conidiogenesis
(Gr- thallos= branch) conidium arises by conversion of a pre-existing
segment of the fungal thallus.
a) Holothallic – All the layers of conidiogeneous cell contribute towards
the formation of conidium wall.
e.g. Microsporium gypseum
b) Thallic-arthric - The conidia are formed by dissolution of septa along a
hypha. e.g Galactomyces candidus (Geotrichum candidum)
Blastic Conidiogenesis
The conidium develops by the blowing out of the wall of cell, usually
from the tip of a hypha.
a) Holoblastic- Conidium elongates and swells before being cut off by
septum. Both inner and outer layer of conidiogeneous cells are
involved, e.g. Cladosporium, Sclerotinia fructigena.
b. Enteroblastic- Only the inner wall layers of the conidiogenous cell
are involved in conidium formation.
i. Tretic –Conidia develops by protrusion of inner wall through a
channel in the outer wall.
Monotretic Alternaria Polytretic Curvularia
ii. Phialide
(Gr. phialis = flask), usually shaped
like a bottle with a narrow neck.
Conidiogeneous cell wall with an
open end through which a
basipetal succession of conidia
(phialospore) develops.
e.g. Penicillium, Aspergillus
iii. Annellide
(Lat. Annulus = little rings)
A conidiogeneous cell that extends
and retracts as it produces conidia
often leaving a succession of scars
or rings.
e.g. Scopulariopsis
Development of conidial loci
- Cole (1986)
Arrangement of conidia at locus
Seriate
A. Solitary-Conidia
formed singly or
in chains
Conidia produced in
groups at a locus
B. Catenate- true chains
are formed with a
conidial wall layers in
continuity.
C. False chain
D. Spore
heads
Conidial succession
 Basipetal
Youngest conidium at the base. e.g.
Penicillium.
 Acropetal
Youngest conidium is at the tip.
e.g. Alternaria, Cladosporium.
Dehisence of Conidia
A. Schizolytic dehisence -(Gr. schizo= to split, divide). Shizolytic
dehisence occur when the two wall layers of delimiting septum
seperate e.g. majority of Ascomycetes.
B. Rhexolytic dehisence - (Gr. rhexis = a rupture, breaking) The
entire septum seperates with conidium, often tearing the cell
directly below. e.g. Onygenales
Types of Conidial Development
Type I - Blastic-Acropetal or Blastic-Synchronous conidiogenesis
Type II - Blastic-Sympodial conidiogenesis
Type III - Blastic-Annellidic or Blastic-Percurrent conidiogenesis
Type IV - Blastic-Phialidic conidiogenesis
Type V - Blastic-Retrogressive conidiogenesis
Type VI - Basauxic conidiogenesis
Type VII - Thallic-Arthric conidiogenesis
Type VIII - Thallic-Solitary conidiogenesis
-By S.J. Hughes
Type I - Blastic-Acropetal or Blastic-Synchronous
Conidiogenesis
Type II - Blastic-Sympodial conidiogenesis
Blastic acropetal -Cladosporium
anamorph of Mycosphaerella tassiana
Blastic synchronous-
Botrytis and Gonatobotryum
Leptographium anamorph of
Ophiostoma
Beauveria
Type III - Blastic-annellidic or Blastic-percurrent
conidiogenesis
Type IV : Blastic-phialidic conidiogenesis
Spilocaea anamorph of Venturia inaequalis.
Penicillium, Aspergillus, Fusarium, Trichoderma
Type V: Blastic-retrogressive conidiogenesis
Type VI: Basauxic conidiogenesis
e.g. Basipetospora, Trichothecium and Cladobotryum.
e.g. Oidium anamorph of Erysiphe spp.
Type VII: Thallic-arthric conidiogenesis
Type VIII: Thallic-solitary conidiogenesis
e.g. Geotrichum anamorphs of Dipodascus spp. (Saccharomycetes), Coremiella
e.g. Microsporum anamorphs of Nannizzia
Hughes’s Classification
S.J. Hughes 1953 classified Hyphomycetes based on conidial
ontogeny i.e. their origin :-
1. Blastospore: Conidia borne as
buds or blown out tips.e.g.
Cladosporium,Torula.
2. Gangliospore: Conidia developed
from the swollen tip of the
conidiophore. e.g. Scopulariopsis,
Amblyosporium.
3. Porospore: Single or whorl of thick
walled conidia formed through one
or more small pores in sporophore
wall .e.g. Alternaria, Curvularia,
Helminthosporium
4. Phialospore: Conidia borne on short
unicellular, oval or sub cylindeical or
flask shaped phialides with a distinct
basal swelling and a narrow distal
neck.e.g. Aspergillus, Penicillium.
5. Arthrospore: Conidia formed by
breaking of hypha into cells. e.g.
Geotrichum.
6. Aleuriospores: Conidia borne on blown
out ends of hyphal tip and cut off by
septum. e.g. Trichothecium.
7. Redulospore: Conidia borne on little
pegs/ sterigmata on surface of
conidiphore. e.g. Botrytis, Ramularia.
CONCLUSION
The conidial fungi demonstrate a broad range of
morphogentic complexity in which the hypha is a
fundamental element from which reproductive
structures (conidia) are derived. In both thallic
and blastic development the hyphal tip is the site
of pivotal events which leads directly or indirectly
to conidium differentiation.
References
Alexopolous C. J., Mims C. W., & Blackwell M.
Introductory Mycology, John Wiley & Sons, Inc.
pp 219-233
Aneja K.R. and R.S. Mehrotra 1990. An
Introduction to Mycology, New Age
International Publisher. pp 417-423
Cole G. T. 1986. Models of Cell Differentiation in
Conidial Fungi, Microbiological Reviews, 50(2),
pp 95-132
Cole G. T., and Kendrick, W. 1981. Biology of
Conidial Fungi Vol 2., Academic Press New
York. pp 272-322
Thank you

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Conidial ontogeny akanksha

  • 1. Presented by- Akanksha Shukla Dept. Of Plant Pathology Conidial Ontogeny
  • 2. Contents  Introduction  Saccardoan spore types  Conidial Ontogeny  Types of Conidium development  Hughes’s Classification  Conclusion
  • 3. Introduction Deuteromycetes fungi are the septate, mycelial fungi which reproduce exclusively by asexual means and are known as imperfect fungi or anamorphic fungi. They lack sexual stage (telomorph) and are believed to represent the conidial stage of higher fungi. The asexual reproduction is through conidia (mitospore). There is a tremendous variety of morphologically different conidia produced. The first attempt to classify these conidial fungi was of Saccardo’s. The study of development of conidia based on its origin is referred to as “Conidial Ontogeny”. The two principle types of conidum ontogeny are ‘Thallic’ and ‘Blastic’. Hughes proposed eight different types of conidium development based on its origin.
  • 4. Saccardoan Spore Types P.A. Saccardo (1845-1920)  “Sylloge Fungorum” (1882-1972)  Developed first system of classifying Hyphomycetes fungi based on morphology of spores:- Color  Hyaline or bright (hyalo-)  Pigmented (phaeo-) Shape and Septation 1. Amerosporae: Conidia non septate, spherical, ovoid to elongated.
  • 5. 2. Didymosporae: conidia two-celled, ovoid to oblong. 3. Phragmosporae: conidia 3 or more celled by transverse septa. 4. Dictyosporae: conidia divided by both transverse and longitudinal septa(muriform).
  • 6. 5. Scolecosporae: conidia slender, filiform, one to several celled; hyaline or coloured. 6. Helicosporae (Allantosporae): Conidia cylindrical, curved (allantoid); hyaline or coloured. 7. Staurosporae: Conidia stellate (star-shaped), radiate or trifurate, one to several celled; hyaline or coloured.
  • 7. Conidial Ontogeny  Conidia/Conidiospores -(sing. conidium) Conidia may be defined as asexual, non-motile spore that belong to the anamorphic stage of a fungus life cycle. The word is derived from the Greek konis ‘dust’ + the diminutive suffix -idium (Sutton, 1986).  Conidiophore -Specialized hyphae that bears one or more conodiogeneous cells.  Conidiogeneous cell - The hyphal cell from which a conidium is formed directly.  Conidiogenesis - The process of formation of conidium.  Conidia may originate from conidiogeneous cells by either of the two ways:- 1. Thallic 2. Blastic
  • 8. Thallic Conidiogenesis (Gr- thallos= branch) conidium arises by conversion of a pre-existing segment of the fungal thallus. a) Holothallic – All the layers of conidiogeneous cell contribute towards the formation of conidium wall. e.g. Microsporium gypseum b) Thallic-arthric - The conidia are formed by dissolution of septa along a hypha. e.g Galactomyces candidus (Geotrichum candidum)
  • 9. Blastic Conidiogenesis The conidium develops by the blowing out of the wall of cell, usually from the tip of a hypha. a) Holoblastic- Conidium elongates and swells before being cut off by septum. Both inner and outer layer of conidiogeneous cells are involved, e.g. Cladosporium, Sclerotinia fructigena.
  • 10. b. Enteroblastic- Only the inner wall layers of the conidiogenous cell are involved in conidium formation. i. Tretic –Conidia develops by protrusion of inner wall through a channel in the outer wall. Monotretic Alternaria Polytretic Curvularia
  • 11. ii. Phialide (Gr. phialis = flask), usually shaped like a bottle with a narrow neck. Conidiogeneous cell wall with an open end through which a basipetal succession of conidia (phialospore) develops. e.g. Penicillium, Aspergillus iii. Annellide (Lat. Annulus = little rings) A conidiogeneous cell that extends and retracts as it produces conidia often leaving a succession of scars or rings. e.g. Scopulariopsis
  • 12. Development of conidial loci - Cole (1986)
  • 13. Arrangement of conidia at locus Seriate A. Solitary-Conidia formed singly or in chains Conidia produced in groups at a locus B. Catenate- true chains are formed with a conidial wall layers in continuity. C. False chain D. Spore heads
  • 14. Conidial succession  Basipetal Youngest conidium at the base. e.g. Penicillium.  Acropetal Youngest conidium is at the tip. e.g. Alternaria, Cladosporium.
  • 15. Dehisence of Conidia A. Schizolytic dehisence -(Gr. schizo= to split, divide). Shizolytic dehisence occur when the two wall layers of delimiting septum seperate e.g. majority of Ascomycetes. B. Rhexolytic dehisence - (Gr. rhexis = a rupture, breaking) The entire septum seperates with conidium, often tearing the cell directly below. e.g. Onygenales
  • 16. Types of Conidial Development Type I - Blastic-Acropetal or Blastic-Synchronous conidiogenesis Type II - Blastic-Sympodial conidiogenesis Type III - Blastic-Annellidic or Blastic-Percurrent conidiogenesis Type IV - Blastic-Phialidic conidiogenesis Type V - Blastic-Retrogressive conidiogenesis Type VI - Basauxic conidiogenesis Type VII - Thallic-Arthric conidiogenesis Type VIII - Thallic-Solitary conidiogenesis -By S.J. Hughes
  • 17. Type I - Blastic-Acropetal or Blastic-Synchronous Conidiogenesis Type II - Blastic-Sympodial conidiogenesis Blastic acropetal -Cladosporium anamorph of Mycosphaerella tassiana Blastic synchronous- Botrytis and Gonatobotryum Leptographium anamorph of Ophiostoma Beauveria
  • 18. Type III - Blastic-annellidic or Blastic-percurrent conidiogenesis Type IV : Blastic-phialidic conidiogenesis Spilocaea anamorph of Venturia inaequalis. Penicillium, Aspergillus, Fusarium, Trichoderma
  • 19. Type V: Blastic-retrogressive conidiogenesis Type VI: Basauxic conidiogenesis e.g. Basipetospora, Trichothecium and Cladobotryum. e.g. Oidium anamorph of Erysiphe spp.
  • 20. Type VII: Thallic-arthric conidiogenesis Type VIII: Thallic-solitary conidiogenesis e.g. Geotrichum anamorphs of Dipodascus spp. (Saccharomycetes), Coremiella e.g. Microsporum anamorphs of Nannizzia
  • 21. Hughes’s Classification S.J. Hughes 1953 classified Hyphomycetes based on conidial ontogeny i.e. their origin :- 1. Blastospore: Conidia borne as buds or blown out tips.e.g. Cladosporium,Torula. 2. Gangliospore: Conidia developed from the swollen tip of the conidiophore. e.g. Scopulariopsis, Amblyosporium. 3. Porospore: Single or whorl of thick walled conidia formed through one or more small pores in sporophore wall .e.g. Alternaria, Curvularia, Helminthosporium
  • 22. 4. Phialospore: Conidia borne on short unicellular, oval or sub cylindeical or flask shaped phialides with a distinct basal swelling and a narrow distal neck.e.g. Aspergillus, Penicillium. 5. Arthrospore: Conidia formed by breaking of hypha into cells. e.g. Geotrichum. 6. Aleuriospores: Conidia borne on blown out ends of hyphal tip and cut off by septum. e.g. Trichothecium. 7. Redulospore: Conidia borne on little pegs/ sterigmata on surface of conidiphore. e.g. Botrytis, Ramularia.
  • 23. CONCLUSION The conidial fungi demonstrate a broad range of morphogentic complexity in which the hypha is a fundamental element from which reproductive structures (conidia) are derived. In both thallic and blastic development the hyphal tip is the site of pivotal events which leads directly or indirectly to conidium differentiation.
  • 24. References Alexopolous C. J., Mims C. W., & Blackwell M. Introductory Mycology, John Wiley & Sons, Inc. pp 219-233 Aneja K.R. and R.S. Mehrotra 1990. An Introduction to Mycology, New Age International Publisher. pp 417-423 Cole G. T. 1986. Models of Cell Differentiation in Conidial Fungi, Microbiological Reviews, 50(2), pp 95-132 Cole G. T., and Kendrick, W. 1981. Biology of Conidial Fungi Vol 2., Academic Press New York. pp 272-322