SGM Carbon Dioxide Incubator are designed to provide ideal tissue culturing conditions with high resistance to contamination.
It gives control over contamination with simplicity of operation and reliable performance
Romanowsky staining or Romanowsky–Giemsa staining, is a prototypical staining technique, widely used in hematology and cytopathology.
They are used to differentiate cells for microscopic examination in air dried cytological smears or pathological specimens, especially blood and bone marrow films, and to detect parasites such as malaria within the blood.
Romanowsky stains is a neutral dye containing both acid and basic dyes in combination. It contains both azure B (electron acceptor) and eosin Y (electron donor).
The value of Romanowsky staining lies in its ability to produce a wide range of hues, allowing cellular components to be easily differentiated. This phenomenon is referred to as the Romanowsky effect, or more generally as metachromasia.
These stains allow better estimation of cell size, nuclear size, cell cytoplasm and identify ground substances by metachromasia.
Cell blocks are an integral part of cytology preparations and ancillary testing.
In certain settings, such as molecular testing of lung cancer or by a commercial laboratory, they are the preferred cytology preparation.
To optimize them, care in specimen procurement, triage, and improvement in current processing techniques are necessary.
Cytopathology Lab manual for MLT Students Vamsi kumar
COURSE OUTCOMES
On completion of this course the students will able to:
Understand the preparation of Cytopathological reagents.
Wet film preparation.
Staining (H&E, Pap) of Vaginal, Cervical, Sputum, FNAC Etc.
Role of serum and supplements in culture medium k.skailash saini
ROLE OF SERUM AND SUPPLEMENTS IN CULTURE MEDIA
Serum is a complex mix of albumins, growth factors and growth inhibitors.
Serum is one of the most important components of cell culture media and serves as a source for amino acids, proteins, vitamins (particularly fat-soluble vitamins such as A, D, E, and K), carbohydrates, lipids, hormones, growth factors, minerals, and trace elements.
Serum from fetal and calf bovine sources are commonly used to support the growth of cells in culture.
Fetal serum is a rich source of growth factors and is appropriate for cell cloning and for the growth of fastidious cells.
Calf serum is used in contact-inhibition studies because of its lower growth-promoting properties.
Normal growth media often contain 2-10% of serum.
Supplementation of media with serum serves the following functions :
Serum provides the basic nutrients (both in the solution as well as bound to the proteins) for cells.
Serum provides several growth factors and hormones involved in growth promotion and specialized cell function.
It provides several binding proteins like albumin, transferrin, which can carry other molecules into the cell. For example: albumin carries lipids, vitamins, hormones, etc. into cells.
It also supplies proteins, like fibronectin, which promote the attachment of cells to the substrate. It also provides spreading factors that help the cells to spread out before they begin to divide.
It provides protease inhibitors which protect cells from proteolysis.
It also provides minerals, like Na+, K+, Zn2+, Fe2+, etc.
It increases the viscosity of the medium and thus, protects cells from mechanical damages during agitation of suspension cultures.
It also acts a buffer.
Due to the presence of both growth factors and inhibitors, the role of serum in cell culture is very complex.
Unfortunately, in addition to serving various functions, the use of serum in tissue culture applications has several drawbacks .
This method is used to visualise the localisation and quantity of a protein of interest. The target protein is bound to by a specific primary antibody, which in turn is detected by a secondary antibody conjugated to a fluorophore. A fluorescent or confocal microscope is used to visualise the protein.
Immunocytochemistry (ICC) differs from immunohistochemistry (IHC) in that the former is performed on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed. In contrast, immunohistochemical samples are sections of biological tissue, where each cell is surrounded by tissue architecture and other cells normally found in the intact tissue. These differences cause the samples to be prepared differently. For ICC, the sample requires permeabilisation so that the antibodies can reach the intracellular targets. Depending on the thickness of the sample, IHC samples do not require this.
Do you have a technical question? Get in touch: info@stjohnslabs.com
Automation in histopathology or advance technology in histopathology labSamim Bashir
now a day all work is done by machines .there is alot of new machines which work autmatically in hisopathology.they reduce human effort .they also increase the speed of work flow
EducationUSA Weekly Update, #358, December 9, 2013EducationUSA
Accredited U.S. educational institutions can publicize their financial aid opportunities and campus news to the EducationUSA network of over 400 centers in 170 countries through our free service: EdUSA Weekly Update. EducationUSA advising centers will, in turn, circulate this information through a multitude of international channels via social media, newsletters, bulletin boards, websites, and more!
Romanowsky staining or Romanowsky–Giemsa staining, is a prototypical staining technique, widely used in hematology and cytopathology.
They are used to differentiate cells for microscopic examination in air dried cytological smears or pathological specimens, especially blood and bone marrow films, and to detect parasites such as malaria within the blood.
Romanowsky stains is a neutral dye containing both acid and basic dyes in combination. It contains both azure B (electron acceptor) and eosin Y (electron donor).
The value of Romanowsky staining lies in its ability to produce a wide range of hues, allowing cellular components to be easily differentiated. This phenomenon is referred to as the Romanowsky effect, or more generally as metachromasia.
These stains allow better estimation of cell size, nuclear size, cell cytoplasm and identify ground substances by metachromasia.
Cell blocks are an integral part of cytology preparations and ancillary testing.
In certain settings, such as molecular testing of lung cancer or by a commercial laboratory, they are the preferred cytology preparation.
To optimize them, care in specimen procurement, triage, and improvement in current processing techniques are necessary.
Cytopathology Lab manual for MLT Students Vamsi kumar
COURSE OUTCOMES
On completion of this course the students will able to:
Understand the preparation of Cytopathological reagents.
Wet film preparation.
Staining (H&E, Pap) of Vaginal, Cervical, Sputum, FNAC Etc.
Role of serum and supplements in culture medium k.skailash saini
ROLE OF SERUM AND SUPPLEMENTS IN CULTURE MEDIA
Serum is a complex mix of albumins, growth factors and growth inhibitors.
Serum is one of the most important components of cell culture media and serves as a source for amino acids, proteins, vitamins (particularly fat-soluble vitamins such as A, D, E, and K), carbohydrates, lipids, hormones, growth factors, minerals, and trace elements.
Serum from fetal and calf bovine sources are commonly used to support the growth of cells in culture.
Fetal serum is a rich source of growth factors and is appropriate for cell cloning and for the growth of fastidious cells.
Calf serum is used in contact-inhibition studies because of its lower growth-promoting properties.
Normal growth media often contain 2-10% of serum.
Supplementation of media with serum serves the following functions :
Serum provides the basic nutrients (both in the solution as well as bound to the proteins) for cells.
Serum provides several growth factors and hormones involved in growth promotion and specialized cell function.
It provides several binding proteins like albumin, transferrin, which can carry other molecules into the cell. For example: albumin carries lipids, vitamins, hormones, etc. into cells.
It also supplies proteins, like fibronectin, which promote the attachment of cells to the substrate. It also provides spreading factors that help the cells to spread out before they begin to divide.
It provides protease inhibitors which protect cells from proteolysis.
It also provides minerals, like Na+, K+, Zn2+, Fe2+, etc.
It increases the viscosity of the medium and thus, protects cells from mechanical damages during agitation of suspension cultures.
It also acts a buffer.
Due to the presence of both growth factors and inhibitors, the role of serum in cell culture is very complex.
Unfortunately, in addition to serving various functions, the use of serum in tissue culture applications has several drawbacks .
This method is used to visualise the localisation and quantity of a protein of interest. The target protein is bound to by a specific primary antibody, which in turn is detected by a secondary antibody conjugated to a fluorophore. A fluorescent or confocal microscope is used to visualise the protein.
Immunocytochemistry (ICC) differs from immunohistochemistry (IHC) in that the former is performed on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed. In contrast, immunohistochemical samples are sections of biological tissue, where each cell is surrounded by tissue architecture and other cells normally found in the intact tissue. These differences cause the samples to be prepared differently. For ICC, the sample requires permeabilisation so that the antibodies can reach the intracellular targets. Depending on the thickness of the sample, IHC samples do not require this.
Do you have a technical question? Get in touch: info@stjohnslabs.com
Automation in histopathology or advance technology in histopathology labSamim Bashir
now a day all work is done by machines .there is alot of new machines which work autmatically in hisopathology.they reduce human effort .they also increase the speed of work flow
EducationUSA Weekly Update, #358, December 9, 2013EducationUSA
Accredited U.S. educational institutions can publicize their financial aid opportunities and campus news to the EducationUSA network of over 400 centers in 170 countries through our free service: EdUSA Weekly Update. EducationUSA advising centers will, in turn, circulate this information through a multitude of international channels via social media, newsletters, bulletin boards, websites, and more!
Fairytales | Книга Сказок Orby (авторы - дети, призеры акции "Я хочу путешест...Orby
Книга Сказок Orby (авторы - дети, призеры акции "Я хочу путешествовать" и конкурса на самую модную сказку). Не для продажи.
Получить Книгу Сказок в подарок можно в фирменных магазинах детской дизайнерской одежды Orby. Подробности в местах продаж.
Direct Heat Microbiological C02 Incubators provide a reliable controlled in-vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage and preservation of gametes and animal tissue cell cultures intended for research at body temperature or under hypoxic conditions.
Direct heat Co2 microbiological incubators provide a reliably controlled in vitro environment for optimal tissue development of cell cultures. The chamber also provides an environment for the storage and preservation of gametes and Cultures of animal tissue cells for research purposes at body temperature or under hypoxic conditions. a unique blend
Product features to promote cell growth through precise control of humidity.
We are manufacturers of CO2 incubators, Carbon Di Oxide Incubators Carbon Dioxide Incubators in India since 1990. Our CO2 incubators are widely used in various applications that require ideal conditions for tissue culture and maximum security from contaminations.For More Information Please Logon http://goo.gl/AW4rhX
Acmas Technologies (Pvt) Ltd.,a research laboratory equipment manufacturing company has expanded its goal to become a one-stop shop for research laboratory equipment and instrument with the recent addition of several range of new products in their online catalog.
Medical Waste Incinerator | Biomedical Hospital Incinerator Manufactures - Mi...Andrewfinn01
The medical waste incinerator is designed for primary and secondary combustion systems. Total Organic carbon content in the slag/bottom ash is less than 1% or their loss on ignition is less than 3% of the dry weight as prescribed. The double chamber incinerator is preferably be designed on “controlled-air” incineration principle, as particulate matter emission is low in such incinerator. Air supply in the primary and secondary chamber may be regulated between 30%-80% and 170%-120% of stiochiometric requirement respectively. In primary chamber, primary air is admitted near. Suitable flow measurement device is provided on the primary and secondary air ducting. The combustion air is supplied through a separate forced draft fan after accounting for the air supplied through burners. The primary chamber ts to avoid leakage of gaseous emissions from the chamber and for safety reasons.
Weiber offers an ideal apparatus for conditioning materials at non-ambient temperatures. Now researchers,
engineers, and technicians have a simple and cost effective method to condition samples prior to testing.
SGM has developed a wide variety of clean room equipments designed to meet diverse applications in the life science, clinical, pharmaceutical and industrial laboratory. SGM Clean room system combines the need for a flexible solution with the request for durability and flush surfaces. It offers an economical approach to your cleanroom needs.
CO2 Incubator is designed and manufactured using the accumulated expertise and knowledge that Esco has developed with over 35 years of world class equipment for laboratory usage. Designed with sample safety, energy efficiency and user-friendliness in mind, the system achieves precise parameter controls, highly effective contamination control and intuitive user interfaces all--supported by Esco's solution-based sales and service representatives worldwide.
We are manufacturing Weiber BOD Incubators and Lab Ovens for varied usages in various research and R&D laboratories across India and abroad. We are committed to provide international standard Bacteriological Incubator at reasonable prices, working closely with the industry captains and leading scientists to continuously improve and enhance the quality, reliability and efficiency of our products by incorporating various inputs provided by them, from time to time. For More Information Please Logon http://goo.gl/Xa4Je1
Cross Technical Solutions is an Irish company Specialising in the design, Installation, Service and Maintenance of Specialist Refrigeration systems.
Established with the Pharmaceutical & Food Industry and specialist refrigeration fields in mind we can provide a complete ‘Turn-Key’ package using in-house engineers and the latest design and drawing packages.
It is constructed in compliance with IEC, IS 9000.
eep Freezers / Ultra Deep Freezers is intended for performing different type of test where the temperature of sample environment must be controlled within certain limits.
Facebook : https://www.facebook.com/IsotechTechnology
YT Sub Link: https://bit.ly/isotech-YT-channel
Linkedin : https://in.linkedin.com/company/isotech-technology
Read More : https://www.isotechpl.com/de-humidifier
Sigma Test and Research Centre has been inspected & declared acceptable Contract Testing Laboratory by USFDA (United States Food & Drug Administration). The Laboratory has got testing facility in line with the requirements of international regulatory agencies for analysis of pharmaceutical and food products.
A BCA can also be a useful and cost-effective device when you are involved about tenant health, comfort and security, or about increasing and regular repair expenses on the service, or issue about rule conformity.
Working on the basis of the existing requirements, guidelines, recommendations and legal guidelines, our analyze technicians and professionals – along with in fire our professionals protection, air contamination control, air flow, fridge and air-conditioning technological innovation, dust removal and narrow technology – analyze, assess and assess plants and set ups. Their aim is to provide incorporated solutions to the customer from a single provider.
SGM automatic weather station is an automated version of the traditional weather station, either to save human labor or to enable measurements from remote areas.
A Hollow Cathode Lamp is a light source primarily used for scientific purposes. These lamps don’t really exist outside of laboratory settings, meaning most people will have never seen one. Most of the time, a hollow cathode lamp is used as a method of tuning in specific light frequencies
The rebound value is read from a graduated scale and is designated as the rebound number or rebound index. The compressive strength can be read directly from the graph provided on the body of the hammer
The testing of building strength does not depend on just its life but also on the way it is constructed and the care that is given to it,” construction material.
Proving rings are calibrated in accordance with procedures established by the American Society for Testing and Materials in a standard designated as ASTM E 74 – 95 entitled “Standard Practice of Calibration of Force-Measuring Instruments for Verifying the Force Indication of Testing Machines.
Voltmeter measures voltage across a known source of potential. The voltage referred on the scale of the voltmeter refers to full-scale deflection (FSD). The FSD indication is always on the right hand side of the instrument.
Temperature Controller is one of the important components of every industry and equipment where temperature maintenance, control and monitoring are needed.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
DevOps and Testing slides at DASA ConnectKari Kakkonen
My and Rik Marselis slides at 30.5.2024 DASA Connect conference. We discuss about what is testing, then what is agile testing and finally what is Testing in DevOps. Finally we had lovely workshop with the participants trying to find out different ways to think about quality and testing in different parts of the DevOps infinity loop.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
2. Co2 incubators
• SGM Carbon Dioxide Incubator are designed
to provide ideal tissue culturing conditions
with high resistance to contamination.
• It gives control over contamination with
simplicity of operation and reliable
performance.
3. • SGM CO2 Incubators are used in biological
experiments to allow growth of organisms in
optimal conditions of temperature, carbon
dioxide and humidity.
• Incubators are generally supplied in one of
three configurations, air jacket, dry wall and
water jacket.
4. Material Of Construction :
• Most versatile for Biochemical Oxygen Demand
determinations. Castor wheel mounted cabinet is
double walled with inner chamber of Stainless steel
sheet of grade SS 304 and outer wall of heavy gauge
PCRC steel.
• sheet duly degreased and pre treated with primers
for rust proofing duly painted with attractive stove
enamel or powder coated.
5. Temperature Range :
• 5 Degree C above ambient to 60 degree C
Temperature Control :
The temperature inside the CO2 incubator is
controlled by intelligent programmable
temperature controller and indicator.
