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Classification of Bacteria
Survey of Clinically Relevant Bacteria
Modern Prokaryotic Classification
Eubacteria
Archeabacteria
Cyanobacteria
Thermophiles
We will not forget the Archaea
Have no cell nucleus or any other
membrane organelles within their
cells.
In the past they were viewed as an
unusual group of bacteria and named
archaebacteria but since the Archaea
have an independent evolutionary
history and show many differences in
their biochemistry from other forms
of life.
They are now classified as a separate
domain
Diversity of Bacteria
Classification of Bacteria
 Classification – ordering
 Nomenclature – naming
 Often immortalizes the person
who discovered it or its origin
◦ Escherichia coli  Theodor
Escherich
◦ coli  from colon
 Distinguishing –identification
Classification of Bacteria
 *Kingdom
 Phylum
 Class
 Order
 Family
 *Genus ( 1st name)
 *Species ( 2nd name identifier)
Remember: King Philip Came
Over For Good Spaghetti
Classification of Bacteria
 Morphology – shape, color,
gram specificity
 Metabolism
 Molecular techniques –
Forensics, DNA finger
prints, RNA, protein
analysis
1 Gram Negative Spiral Bacteria
 Slender and flexible, come in a
lot of different shapes
 More rigid than spirochetes
 Ex. – Campylobacter jejuni
◦ Symptom – tenesmus: the
sensation of desire to
defecate, which is common
and occurs frequently , with
out the production of
significant amounts of feces
(often small amounts of
mucous or blood are alone
passed).
2 Gram Negative Spirochetes
 pathogenic
 very flexible
 tightly coiled, helically coiled
 Example
◦ syphilis
Treponema pallidum
Gram Negative Spirochetes
 Most of pathogenic
 Very flexible
 Tightly coiled, helically coiled
 Example
◦ Lyme disease
Borrelia burgdorferi
(organism gets lodged in
tissues)
3 Gram Negative Aerobic Rods
◦ Legionella pneumophila
 Lower respiratory tract
infection
 Needs oxygen
Gram Negative Aerobic Rods
Bordetella pertussis –
whooping cough
 Needs oxygen
Gram Negative Aerobic Rods
◦ Pseudomonas aeruginosa
(pigmented)
 Needs moisture
 Common in hospitals
 Opportunistic pathogen –
causes UTI, skin, and lung
infection
Vibrio
◦ V. cholerae
 Most well known of group
 Very severe dysentery. Can lose
10-15 liters of water/day. Leads
to hypovolemia – low water, hardly
any water in body
◦ V. vulnificus
 Very pathogenic
 Can cause flesh eating disease,
if it gets in a wound
◦ V. parahaemolyticus
 Found in shellfish – oysters
 Halophile – loves salt (will find in oceans, estuaries)
 Self limiting
4 Gram Negative Facultative Rods
Gram Negative Facultative Rods
 Enteric
◦ Salmonella
◦ Shigella
◦ E. coli (0157H7)
5 Gram Negative Anaerobic Rods
 Fusobacterium
◦ Live in between teeth and
gums
◦ Cause tooth abscesses
and periodontal disease
◦ Teeth have nothing to
anchor – bone is
destroyed

6 Gram Negative Cocci or Coccobaccilli
(plump rods)
Neisseria gonorrhoeae –
Usually a diplococcus in
PMN
Sexually Transmitted
Disease
very antibiotic resistant
Gram Negative Cocci or Coccobaccilli (plump
rods)
 Neisseria meningitidis
very infectious and
communicable.
Gram Negative Cocci or Coccobaccilli (plump
rods)
Acinetobacter baumanni iv.
lwoffi
opportunistic, UTI, skin,
and upper respiratory
7 Chlamydia Gram Negative Rods (Transitional)
 Very short little rods
 Gram negative
 Transitional – doesn’t hold stain
well
 Do not have the ability to
synthesize own ATP, therefore and
obligate intracellular parasite of
other animals (humans)
 Can go asymptomatic for a long
time
 Ex.
◦ C. trachomatis – STD, causes eye
infection
◦ C. psittaci – parrot (associated
with birds)
8 Rickettsia Gram Negative Rod (Transitional)
 Small gram negative rods
 Transitional – doesn’t hold
stain well
 Can’t synthesize it’s own
NAD, coenzyme A, therefore
an obligate intracellular
parasite
 Causative agent of Rocky
Mountain Spotted Fever
 Example
◦ R. Prowazekii
9 Mycoplasma Gram Positive (Transitional )
 Gram positive – only because
they take in dye in cell
membrane but it washes away
 Transitional – doesn’t hold stain
well.
 Have no cell wall
 Can not treat with penicillin
 Ex.
◦ Mycoplasma pneumoniae – causes
LRTI
◦ Ureaplasma urealyticum – causes UTI
◦ Both imbed themselves in the tissue.
The most cell damage is done by the
immune system destroying the
tissue.
10 Gram Positive Cocci
Staphyloccocus aureus
MRSA
These bacteria can break
down all tissues of body.
Gram Positive Cocci
Streptococcus pyogenes –
no antibiotic resistance
right now
These bacteria can break
down all tissues of body.
11 Gram positive Endospore Forming Rods
 Difficult to get rid of
because of endospores
 Example
◦ Clostridium tetani
Gram positive Endospore Forming Rods
 Difficult to get rid of
because of endospores
 Example
 C. perfringens – gangrene
Gram positive Endospore Forming Rods
 Difficult to get rid of
because of endospores
 Common in hospitals
 Example
 C. difficile
antibiotic associated
pseudmembraneous
enterocolitis
Clostridium difficile
Gram positive Endospore Forming Rods
Bacillus
 B. anthracis – anthrax
 zoonosis
Gram positive Endospore Forming Rods
Bacillus
 B. cereus – food poising
Especially in high carb
foods – rice, vermicelli
 B. thuringiensis – natures
insecticide
12 Coryneforms
 Pleomorphic (many shapes)
 Example
Corynebacterium diphtheriae
13 Mycobacteria
 Gram positive and Acid Fast
Mycobacterium tuberculosis
Respiratory Pathogen
MDR-TB
In the 1950s we sent people
with TB to the sanitariums
Mycobacteria
 Gram positive and Acid Fast
◦ M. avium intracellular complex
(MAC)
 Really bad bug
 Currently no drugs can cure it
 Especially bad for people with
AIDS
 Can cause atypical TB
Mycobacteria
 Gram positive and Acid Fast
◦ M. leprae
 Causative agent of leprosy
 Not very common
 Only affects areas of body
that are below body
temperature
 Natural reservoir is the
armadillo

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Classification of Bacteria Clinically Relevant Bacteria 092410.ppt

  • 1. Classification of Bacteria Survey of Clinically Relevant Bacteria
  • 3. We will not forget the Archaea Have no cell nucleus or any other membrane organelles within their cells. In the past they were viewed as an unusual group of bacteria and named archaebacteria but since the Archaea have an independent evolutionary history and show many differences in their biochemistry from other forms of life. They are now classified as a separate domain
  • 5. Classification of Bacteria  Classification – ordering  Nomenclature – naming  Often immortalizes the person who discovered it or its origin ◦ Escherichia coli  Theodor Escherich ◦ coli  from colon  Distinguishing –identification
  • 6. Classification of Bacteria  *Kingdom  Phylum  Class  Order  Family  *Genus ( 1st name)  *Species ( 2nd name identifier) Remember: King Philip Came Over For Good Spaghetti
  • 7. Classification of Bacteria  Morphology – shape, color, gram specificity  Metabolism  Molecular techniques – Forensics, DNA finger prints, RNA, protein analysis
  • 8. 1 Gram Negative Spiral Bacteria  Slender and flexible, come in a lot of different shapes  More rigid than spirochetes  Ex. – Campylobacter jejuni ◦ Symptom – tenesmus: the sensation of desire to defecate, which is common and occurs frequently , with out the production of significant amounts of feces (often small amounts of mucous or blood are alone passed).
  • 9. 2 Gram Negative Spirochetes  pathogenic  very flexible  tightly coiled, helically coiled  Example ◦ syphilis Treponema pallidum
  • 10. Gram Negative Spirochetes  Most of pathogenic  Very flexible  Tightly coiled, helically coiled  Example ◦ Lyme disease Borrelia burgdorferi (organism gets lodged in tissues)
  • 11. 3 Gram Negative Aerobic Rods ◦ Legionella pneumophila  Lower respiratory tract infection  Needs oxygen
  • 12. Gram Negative Aerobic Rods Bordetella pertussis – whooping cough  Needs oxygen
  • 13. Gram Negative Aerobic Rods ◦ Pseudomonas aeruginosa (pigmented)  Needs moisture  Common in hospitals  Opportunistic pathogen – causes UTI, skin, and lung infection
  • 14. Vibrio ◦ V. cholerae  Most well known of group  Very severe dysentery. Can lose 10-15 liters of water/day. Leads to hypovolemia – low water, hardly any water in body ◦ V. vulnificus  Very pathogenic  Can cause flesh eating disease, if it gets in a wound ◦ V. parahaemolyticus  Found in shellfish – oysters  Halophile – loves salt (will find in oceans, estuaries)  Self limiting 4 Gram Negative Facultative Rods
  • 15. Gram Negative Facultative Rods  Enteric ◦ Salmonella ◦ Shigella ◦ E. coli (0157H7)
  • 16. 5 Gram Negative Anaerobic Rods  Fusobacterium ◦ Live in between teeth and gums ◦ Cause tooth abscesses and periodontal disease ◦ Teeth have nothing to anchor – bone is destroyed 
  • 17. 6 Gram Negative Cocci or Coccobaccilli (plump rods) Neisseria gonorrhoeae – Usually a diplococcus in PMN Sexually Transmitted Disease very antibiotic resistant
  • 18. Gram Negative Cocci or Coccobaccilli (plump rods)  Neisseria meningitidis very infectious and communicable.
  • 19. Gram Negative Cocci or Coccobaccilli (plump rods) Acinetobacter baumanni iv. lwoffi opportunistic, UTI, skin, and upper respiratory
  • 20. 7 Chlamydia Gram Negative Rods (Transitional)  Very short little rods  Gram negative  Transitional – doesn’t hold stain well  Do not have the ability to synthesize own ATP, therefore and obligate intracellular parasite of other animals (humans)  Can go asymptomatic for a long time  Ex. ◦ C. trachomatis – STD, causes eye infection ◦ C. psittaci – parrot (associated with birds)
  • 21. 8 Rickettsia Gram Negative Rod (Transitional)  Small gram negative rods  Transitional – doesn’t hold stain well  Can’t synthesize it’s own NAD, coenzyme A, therefore an obligate intracellular parasite  Causative agent of Rocky Mountain Spotted Fever  Example ◦ R. Prowazekii
  • 22. 9 Mycoplasma Gram Positive (Transitional )  Gram positive – only because they take in dye in cell membrane but it washes away  Transitional – doesn’t hold stain well.  Have no cell wall  Can not treat with penicillin  Ex. ◦ Mycoplasma pneumoniae – causes LRTI ◦ Ureaplasma urealyticum – causes UTI ◦ Both imbed themselves in the tissue. The most cell damage is done by the immune system destroying the tissue.
  • 23. 10 Gram Positive Cocci Staphyloccocus aureus MRSA These bacteria can break down all tissues of body.
  • 24. Gram Positive Cocci Streptococcus pyogenes – no antibiotic resistance right now These bacteria can break down all tissues of body.
  • 25. 11 Gram positive Endospore Forming Rods  Difficult to get rid of because of endospores  Example ◦ Clostridium tetani
  • 26. Gram positive Endospore Forming Rods  Difficult to get rid of because of endospores  Example  C. perfringens – gangrene
  • 27. Gram positive Endospore Forming Rods  Difficult to get rid of because of endospores  Common in hospitals  Example  C. difficile antibiotic associated pseudmembraneous enterocolitis
  • 29. Gram positive Endospore Forming Rods Bacillus  B. anthracis – anthrax  zoonosis
  • 30. Gram positive Endospore Forming Rods Bacillus  B. cereus – food poising Especially in high carb foods – rice, vermicelli  B. thuringiensis – natures insecticide
  • 31. 12 Coryneforms  Pleomorphic (many shapes)  Example Corynebacterium diphtheriae
  • 32. 13 Mycobacteria  Gram positive and Acid Fast Mycobacterium tuberculosis Respiratory Pathogen MDR-TB In the 1950s we sent people with TB to the sanitariums
  • 33. Mycobacteria  Gram positive and Acid Fast ◦ M. avium intracellular complex (MAC)  Really bad bug  Currently no drugs can cure it  Especially bad for people with AIDS  Can cause atypical TB
  • 34. Mycobacteria  Gram positive and Acid Fast ◦ M. leprae  Causative agent of leprosy  Not very common  Only affects areas of body that are below body temperature  Natural reservoir is the armadillo