This document discusses chromogenic substrates, which are peptides linked to a chromophore that are used to detect and measure the activity of proteolytic enzymes like thrombin and factor Xa. Chromogenic substrates are cleaved by these enzymes, releasing the chromophore p-nitroaniline which turns yellow and can be quantified. The document provides examples of specific chromogenic substrates for various enzymes and describes their use in clinical assays to measure coagulation factors, antithrombin, heparin, and activated protein C.
Introduction, Principle, Instrumentation and Applications of SDS-PAGEMohammed Mubeen
The following presentation contains helpful information regarding SDS-PAGE, including the history, introduction, principle, instrumentation, advantages and applications of SDS-PAGE.
Laboratory method for measuring enzyme activity.
Vital for study of enzyme kinetics and enzyme inhibition.
Measurement of enzyme activity – follow the change in concentration of substrate or product – measure reaction rate.
Gel electrophoresis native, denaturing&reducingLovnish Thakur
Electrophoresis is a technique used to separate and sometimes purify macromolecules - especially proteins and nucleic acids - that differ in size, charge or conformation.
Introduction, Principle, Instrumentation and Applications of SDS-PAGEMohammed Mubeen
The following presentation contains helpful information regarding SDS-PAGE, including the history, introduction, principle, instrumentation, advantages and applications of SDS-PAGE.
Laboratory method for measuring enzyme activity.
Vital for study of enzyme kinetics and enzyme inhibition.
Measurement of enzyme activity – follow the change in concentration of substrate or product – measure reaction rate.
Gel electrophoresis native, denaturing&reducingLovnish Thakur
Electrophoresis is a technique used to separate and sometimes purify macromolecules - especially proteins and nucleic acids - that differ in size, charge or conformation.
SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa.
Color Change Temperature (Thermochromic) Indicator ApplicationsJohn Romano
Global leader in the design development and manufacturing of temperature sensitive color changing and chemical reactive temperature measurement labels, indicators and graphics. The company services primarily the industrial and healthcare temperature measure markets with regulatory sensitive products distributed through reseller, private label and OEM channels.
LCR Hallcrest Offers Standard Temperature Indicating Product Portfolios and Designs, Develops & Manufacturers Custom / Private Label Products.
Technology & Capability
• Manufacturing and Research and Development Sites
o Glenview IL & Tampa, Florida
o Deeside & Poole, UK
• Support
o Engineering, Chemical, Regulatory
• Certifications
o CE, ISO13485, CPSIA, ROHS Compliant & FDA Registered
• Brands
o Thermax Irreversible (Permanent Color Change)
o Digitemp Reversible
Core Production Capability
• Microencapsulation (Custom)
• Formulations Inks , Dyes and Masterbatch
• Coating, Screening Printing, Laminating, Die Cutting, Blister Packing
• Labels
Microbiological and Chromogenic Culture Mediaindianoida2012
Microbiological Culture is a method of multiplying microbial organisms by letting them reproduce in predetermined culture media under controlled laboratory conditions. To facilitate and improve the quality of the microbial testing, Titan Media Ltd. developed an original range of chromogenic culture media for a wide range of pathogens named Compass available in both prepared and dehydrated formats.
Titan Media Ltd. guides their customers through a highly ethical and transparent product evaluation process to ensure that specific needs are fully addressed through an informed choice of the proper products from their wide range. Maximum quality control measures are taken throughout the manufacturing process to give the desired results. Titan Media puts greatest effort to provide products of maximum attainable quality to their customers.
For detail please visit -: http://www.titanmedia.in/chromogenic-media-1-22.html
Address:-
TITAN MEDIA
TITAN BIOTECH LTD
A-2/3, 303-305, 3rd Floor, Lusa Tower,
Azadpur Commercial Complex , Azadpur
Delhi - 110033 (India)
Phone: +91-11-27677960, 27674615, 27670742
+91-11-47020100 (30 Lines)
Fax: +91-11-27674181
SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa.
Color Change Temperature (Thermochromic) Indicator ApplicationsJohn Romano
Global leader in the design development and manufacturing of temperature sensitive color changing and chemical reactive temperature measurement labels, indicators and graphics. The company services primarily the industrial and healthcare temperature measure markets with regulatory sensitive products distributed through reseller, private label and OEM channels.
LCR Hallcrest Offers Standard Temperature Indicating Product Portfolios and Designs, Develops & Manufacturers Custom / Private Label Products.
Technology & Capability
• Manufacturing and Research and Development Sites
o Glenview IL & Tampa, Florida
o Deeside & Poole, UK
• Support
o Engineering, Chemical, Regulatory
• Certifications
o CE, ISO13485, CPSIA, ROHS Compliant & FDA Registered
• Brands
o Thermax Irreversible (Permanent Color Change)
o Digitemp Reversible
Core Production Capability
• Microencapsulation (Custom)
• Formulations Inks , Dyes and Masterbatch
• Coating, Screening Printing, Laminating, Die Cutting, Blister Packing
• Labels
Microbiological and Chromogenic Culture Mediaindianoida2012
Microbiological Culture is a method of multiplying microbial organisms by letting them reproduce in predetermined culture media under controlled laboratory conditions. To facilitate and improve the quality of the microbial testing, Titan Media Ltd. developed an original range of chromogenic culture media for a wide range of pathogens named Compass available in both prepared and dehydrated formats.
Titan Media Ltd. guides their customers through a highly ethical and transparent product evaluation process to ensure that specific needs are fully addressed through an informed choice of the proper products from their wide range. Maximum quality control measures are taken throughout the manufacturing process to give the desired results. Titan Media puts greatest effort to provide products of maximum attainable quality to their customers.
For detail please visit -: http://www.titanmedia.in/chromogenic-media-1-22.html
Address:-
TITAN MEDIA
TITAN BIOTECH LTD
A-2/3, 303-305, 3rd Floor, Lusa Tower,
Azadpur Commercial Complex , Azadpur
Delhi - 110033 (India)
Phone: +91-11-27677960, 27674615, 27670742
+91-11-47020100 (30 Lines)
Fax: +91-11-27674181
Smart materials are designed materials that have one or more properties that can be significantly changed in a controlled fashion by external stimuli, such as stress, temperature, moisture, pH, electric or magnetic fields.
Introduction to smart materials and their applications in engineering.
How to prepare MR (Magnetrorheological) fluids at home?
What are Shape Memory Alloys?
What are Piezoelectric materials?
#WikiCourses
https://wikicourses.wikispaces.com/Topic04+Smart+Materials
receptor as drug target (receptor structure and signal transduction)Ravish Yadav
The all the content in this profile is completed by the teachers, students as well as other health care peoples.
thank you, all the respected peoples, for giving the information to complete this presentation.
this information is free to use by anyone.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
3. Chemical structure Prothrombin, the natural substrate of FXa, is cleaved by FXa at two positions, each proceeded by the same four amino acid sequence. FXa activity can be determined by the chromogenic substrate S-2222 which is composed of the same amino acids coupled to a chromophore
26. Antithrombin monitoring chromogenic activity assays Chromogenic heparin cofactor activity assays: - The sample is incubated with heparin and an excess amount of thrombin or FXa. The residual thrombin or FXa then cleaves a chromogenic substrate AT + Heparin [AT*Heparin] [AT*Heparin] + FXa (excess) [AT-FXa- Heparin] + FXa(residual) Chromogenic substrate FXa(residual) Peptide + pNA
27. Antithrombin: anti-FXa assay Sample/Standard dilution: 25 l sample+ 3000 l saline Procedure Volumes Diluted sample/standard 50 l Factor Xa (2.9 nkat/ml in Hep Buffer) 50 l Incubate at 37°C 90 sec S-2765 (0.8 mg/ml) 50 l Read A/min at 405 nm for rate method or add 50 l Acetic acid after 90 sec incubation for end-point method.
36. Protein C in the coagulation system Extrinsic pathway Intrinsic pathway TF FVIIa FIXa FVIIIa PL, Ca2+ FXa FX FVa PL, Ca2+ Prothrombin Thrombin Fibrinogen Fibrin Protein C APC Thrombo-modulin Protein S FV
37. Protein C - an anticoagulant Vitamin K dependent proteins: Anticoagulants: protein C and protein S Procoagulants: Factor II, VII, IX and X Synthesised in the liver Glu Gla (glutamic acid residues are converted to gamma-carboxyglutamic acid) The Gla domain binds calcium ions which form a bridge to the phospholipid surfaces on platelets and endothelial cells.
39. Protein C- the function Protein C inhibits coagulation through inactivation of FVIIIa and FVa Protein C potentiates the fibrinolytic system by inhibiting PAI-1, the major inhibitor of fibrinolysis.
40. Protein C activation Protein C is activated (to APC) by thrombin. A small peptide is removed. Activation by thrombin alone is slow. The complex thrombin-thrombomodulin activates protein C 20 000 times faster. PC APC TM
41.
42.
43.
44. Coamatic Protein C - the principle Protein C APC Protac S-2366 Peptide + pNA APC
48. Substrate selectivity The Chromogenix catalogue includes a section which shows the cross-reactivity of the substrates with the different enzymes tested
49. Kinetic data The Chromogenix catalogue includes a section which shows the kinetic data of the substrates toward the different enzymes tested
Where do we find protein C in the coagulation system? Protein C is an anticoagulant which when activated cleaves FVIIIa and FVa. To do this the two cofactors protein S and FV are needed. Protien C is activiated by thrombin in a complex with thrombomodulin.
Protein C belongs to a class of proteins wich are dependent on vitamin K to become functional. In this class of proteins there are two proteins with anticoagulant properties: protein C and protein S. Also proteins with procoagulant properties belong to this class, namely thrombin, FVII, FIX and FX. All the vitamin K dependent proteins are syntesized in the liver. Vitamin K participates in a reaction in which glutamin acid residues in these proteins are -carboxylated to -carboxyglutamic acid. The region containing the -carboxyglutamic acid residues then binds calcium ions which form a bridge to phospholipids on platelets and endothelial cells. So these proteins need to be bound to phospholipid surfaces to be functional.
So protein C consists of three different regions: -the Gla-domain which interacts with the negatively charged phospholipids in the presence of calcium ions - the EGF-domain which interacts with protein S - the protease domain which contains the active site which cleaves FVIIIa and FVa
The function of protein C is to inhibit coagulation through inactivation of FVIIIa and FVa. The result of this inactivation is decreased thrombin generation. Protein C does not cleave the non-activated forms of FVIII and FV. Protein C also potentiates the fibrinolytic system by inhibiting PAI-1 the major inhibitor of fibrinolysis.
Protein C is a zymogen that is converted to an active serine protease, activated protein C (APC), by thrombin. When activated a small peptide is removed. Thrombin alone can activate protein C but this activation is slow. When thrombin is bound to a protein called thrombomodulin the activiation is 20 000 times faster.
Thrombomodulin is a membrane-protein that is present on the endothelium. The highest concnetration of TM is found in the capillaries, because of the high ratio of endothelial surface to volume of circulating blood. Thrombin that enters the microcirculation will therefore quickly be bound to thrombomodulin and start to activate protein C. TM has three different effects on thrombin: 1. It increases the rate by which thrombin activates protein C 2. It removes the procoagulant properties of thrombin. When bound to TM thrombin does not act on fibrinogen to produce fibrin. 3. TM aslo accelerates the thrombin-antithrombin reaction. So thrombomodulin has got its name becuase it modulates the activity of thrombin from a procoagulant to a anticoagulant.
APC has two known cofactors: protein S and Factor V. Protein S functions as a cofactor for APC by helping APC to bind to platelets or endothelial surfaces via calcium ion bridges. However, protein S in only available for APC when protein S is free of the complement cofactor C4BP to which it is bound while in the circulation. Approximately 60% of protein S in plasma is bound to C4BP. That Factor V is a cofactor to APC was recently discovered. It has been shown that APC degrades FVIII and FVa more effectively when FV is present compared to if only APC and protein S are present.
The functional protein C assay is either a chromogenic assay or a clotting assay. The chromogenic assay is performed in two steps. In the first step the specific protein C activator, Protac, is used to activate the protein C in the plasma. Protac is a serine protease purified from the venom of a snake. It activates protein C without interfering with other coagulation factors. In the next step the activated protein C cleaves a chromogenic substrate.
Coamtic Protein C is performed in two steps In the first step protein C is activated by Protac. The activated protein C then cleaves the chromogenic substrate S-2366.