1. Submitted by: Jonathan Duckworth Contact Number: 01223 394235
Candidate Name: Candidate 116168
Location: Cambridge
Salary Expectations: Currently £31,500
Current Role:
Research Scientist
Desired Role:
Scientist/Senior Scientist
Notice Period:
1 Month
Relevant Experience/Skills For
Role: Holds a PhD in Biochemistry and Molecular Biology, work he has
complemented with over 3 years research experience within
industrial laboratories.
Able to demonstrate experience in the following areas:
 Mammalian and Bacterial cell culture experience.
 Confident developing and validating enzymatic, spectroscopic,
molecular and cellular assays within a drug discovery
environment. (ELISA, qPCR, rtPCR, western blotting).
 Protein expression, purification and characterisation.
 Troubleshooting technical issues and a creative approach to
problem solving.
 Excellent communication skills.
 Experience working under GMP
Happy in his current role but is considering positions elsewhere which
will offer further development.

2. Candidate 116168
Professional Experience:
March 2013 – Present Isogenica, Little Chesterford
Conducting research in a busy biopharmaceuticals laboratory as part of team using various
Molecular biology techniques and display selection techniques in the discovery and
development of cutting edge antibody technology to be used to target drugs for the
treatment of several disease areas. My duties include, but are not restricted to, the
following:
 Phage and CIS-display to screen antibody libraries
 Regular presenting of information and data
 Library validation
 Immunoassay development (ELISA and Western blotting)
 Eukaryotic and bacterial cell culture
 Protein stability studies, e.g. Sypro Orange.
 RT-qPCR
January 2011 – March 2013 MedImmune (SRG), Cambridge
Similar to my current role at Isogenica but also included using ribosome display and FMAT
and the building of optimisation libraries using Kunkel and error-prone (EP) PCR techniques.
Education:
2005 – 2010 School of Chemistry, University of East Anglia
PhD in Biochemistry and Molecular Biology
Title: In vitro and in vivo studies of thio:disulphide oxidoreductase ResA from Bacillus subtilis
and Streptomyces coelicolor.
Supervisor: Dr Nick Le Brun
Project:
 In vivo investigation of key amino acid residues in B. subtilis ResA.
 Design, over expression, purification and in vitro studies of the thiol:disulfide
oxidoreductase ResA from S. coelicolor in Escherichia coli.
 Generation and preliminary studies of a resA gene knockout in S. coelicolor.
All three project elements were designed to further understand the importance and role of
the active site or near active site residues, and to compare the role and properties of ResA
homologues. Funded by the EPSRC.
2002 – 2005 School of Biological Sciences, University of East Anglia
BSc. Molecular Biology and Genetics, Upper Second Class
Dissertation Project: Investigation of proteins that interact with the leader sequence of
periplasmic nitrate reductase from Escherichia coli. Modules studied include; Advanced
Molecular Biology, Plant Molecular Biology, Molecular Microbiology, Parasitology and
Microbial Physiology and Biotechnology.
Research techniques:
 Selection technologies e.g. phage, CIS and ribosome display (with mammalian cells,
purified antigen, epitope competition elution, internalised elution), library design
and creation
 Diagnostic immunology techniques e.g., ELISA and Western blotting

3.  Protein purification, including design of expression vectors, over-expression in cell
culture and purification from cell lysate using the AKTA purification platform and
column chromatography (SEC, IEX, IAC, Unicorn).
 Cell binding assays and screening e.g. FMAT
 Spectroscopic assays including Sypro Orange, UV-visible absorbance and
fluorescence methods to calculate protein unfolding temperatures, pKa values and
redox potentials
 Genetic manipulation, including recombineering, PCR amplification and site directed
mutagenesis, as well as B. subtilis and S. coelicolor genetics.
 SDS-PAGE and haem staining
 Bacterial transformation using chemical competence, electroporation and
conjugative gene transfer.
 Analysing DNA and protein sequence data
 Enzymatic assays, e.g. cytochrome c oxidase assay.
 Cell culture
 Data imaging, manipulation and analytical software (Blaze, PYMOL, Origin, Prism,
Vector NTI, Spotfire)
Research Experience:
Lund University, Sweden.
 As part of my PhD studies, in December 2008, I was granted the opportunity to visit
and conduct research in the Department of Cell and Organism Biology at Lund
University, Sweden, under the guidance of Prof. Lars Hederstedt
Other Experience
Post Graduate Demonstrating
 During the course of my PhD I was involved with the supervision of undergraduate
practical experiments. This involved preparing experimental procedures and
materials, as well as tuition in the theory and techniques involved.
2002 – 2005 Norwich Union Life
Outbound Calling Customer Services Representative
 I was responsible for contacting existing customers of a life insurance provider to
ensure their needs and requests were met.
 I was involved in the training and coaching of other employees.
IT Skills
I have extensive knowledge of the major Microsoft programs including Word, Excel and
Powerpoint. I am also competent in the use of proteomic and genetic analytical and
visualisation tools and advanced internet search engines as well as data analysis software
including Origin, Prism, Vector NTI and Spotfire. I have a full, clean, driving licence.
Publications: On request