this slide provides a overview on various applications of genetic engineering

1. TOPIC : APPLICATIONS OF GENETIC ENGINEERING
Submitted by- Shafali Jaiswal
Navdeep Singh
Ajay Yadav
Mohd Raza
Sandeep Kaur
Section: B1724

2. It is a molecular cytogenetic
technique that uses fluorescent
probes that bind to only those parts
of the chromosome with a high
degree of sequence complementary.
Steps of fish
• Dna unmasking
• Probe and target denaturation
• Probe target hybridisation
• Detection
• Image analysis on fluorescent
microscope

3.  With the help of genetic engineering strain of bacteria and cynobacteria
have been developed which can synthesis ammonia at large scale that can be
used to manufacture fertilizer at much cheaper cost.
 Recombinant DNA tech. can also be used to monitor to degredation of
garbage, petroleum products , nephthalene, and other industrial waste .
 Bacterium pseudomonoas fluorescence genetically altered by transfer of
light producing enzyme called luciferase found in bacterium vibrio fischeri.
 Pseudomonoas fluorescence could cause death of specific worm like
cutworm.

4. Applications Of Genetic Engineering In
Medicine Insulin production

5. Bt cotton is a genetically
modified organism or
genetically modified pest
resistant plant cotton variety,
which produces an insecticide
to bollworm
Bt cotton is made ten year ago,
man’s onto scientists inserted a
toxic gene from the bacterium
called bacillus thuringiensis
into cotton plants to create a
caterpillar resistant variety. The
gene is dna that carries the
instructions for producing toxic
protein.

6. DOLLY SHEEP
 SHEEP A: donate body cell nucleus
 SHEEP B: donate an egg cell without nucleus
 SHEEP C: surrogate mother

7. CLONING DOLLY
 1997- A sheep cloned from an adult
cell
 Nucleus from body cell was inserted
into an empty egg
 Embryo implanted into surrogate
mother
 Sheep is genetic replica of animal
from which body cell was taken.