I am a Science graduate with PhD in Biology and MsC in Biotechnology. During my four years’ degree and as a main research grant contractor I gained invaluable experience in laboratory practices GLP and learnt key techniques such as spectrophotometry, chromatography (HPLC, TLC). I am result driven and motivated person with attention to detail who enjoy working in a challenging and dynamic environment, independently as a part of multidisciplinary team. I feel I have the necessary transferable skills to meet the requirements for work in an industry environment. I am currently enrolled in a professional up skilling program for a Pharmaceutical Industry run by Innopharma Labs in conjunction with ITT Dublin. This course cover topics such as Lean Thinking, Regulatory affairs and GMP, Medice device operation, Aseptic processing, Process Chemistry, Bio process technology, CAPA, Quality Risk Management and Validation.

1. MARIA ZAAK
9 Old Windmill Court, Lower Gerard Griffin Street, Limerick
zaakmaria@gmail.com, 0876324022
PERSONAL PROFILE
I am a Science graduate with PhD in Biology and MsC in Biotechnology. During my four years’ degree
and as a main research grant contractor I gained invaluable experience in laboratory practices and
learnt key techniques such as spectrophotometry, chromatography (HPLC, TLC), ELISA, PAGE (DNA
sampling) and Iwas publishing3timesinELSEVIER® journals.
I am result driven and motivated person with attention to detail who enjoy working in a challenging
and dynamic environment, independently as a part of multidisciplinary team. I feel I have the
necessarytransferableskillstomeetthe requirements forworkinanindustryenvironment.
I am currently enrolled in a professional up skilling program for a Pharmaceutical Industry run by
Innopharma Labs in conjunction with ITT Dublin. This course cover topics such as Lean Thinking,
Regulatory affairs and GMP, Medice device operation, Aseptic processing, Process Chemistry, Bio
processtechnology,CAPA,QualityRiskManagementandValidation.
KEY COMPETENCIES
Technical Skills
 Sample preparation
 Spectrophotometry
 Calibration
 Microscopy
 Analytical Tech
 GLP
 Statistica®(5.5A)
 Chromatography(HPLC,TLC)
 Methoddevelopmentinprocess
sampling
 Lyophilisation
 ELISA
 PAGE (DNA sampling)
Professionals Skills
 Problemsolving
 Attentiontodetail
 Energy& urgent
 Openminded
 Customerfocused
 Organised
 Responsible
 Determined
EDUCATION
2016 Level 8 HETAC UpskillingPharmaceuticalandMedical Device Manufacturing,
InnopharmaLabs& I.T.Tallaght
2003-2008 PhD,Doctor of BiologyScience,Universityof Silesia,Poland
• 2004 Quantitative Methods in Ecotoxicology, Environment Research Institute,
JagiellonianUniversity,Krakow
• 2004 Environmental RiskAssessmentWorkshop,JagiellonianUniversity,Krakow
1998-2003 MSc, Master of Biology Science, Degree Program at Biotechnology of Plants and
Microorganisms,Universityof Silesia,Poland(First-ClassHonours)

2. LABORATORY WORK EXPERIENCE
2007-2005 Researchgrant project: KBN 2P04F 033 30 Poland - Main contractor
Project description: My aim was to investigate the effect of concentration
systemic insecticides (imidaclopride) on the regulation of activity the
detoxification andantioxidationsysteminmidgut.
2003 Researchgrant: KBN 6P04F 085 21 Poland - Participate
Project description: As a part of team I was analysing interrelationship between
the qualityof foodon reproductionaswell asefficiencyof antioxidative defence.
WORK EXPERIENCE
2016-2008 Heatonsand Sport,WorldStore,Limerick,Salesassistant
2008 DunnesStores, ChildersRoad,Limerick,Salesassistant
2008-2007 Green’sCafé andRestaurant,Limerick,Waitress
2003-2007 Laboratories research during PhD studying and delivered lecture to Master
students, University of Silesia, Poland, Department of Animals Physiology and
Ecotoxicology
• I assayed the activity of antioxidation, metabolism and detoxification enzymes,
checked concentration and consistent of xenobiotics (pesticides, allelochemicals,
high metals) using chromatography (HPLC, TLC, GC) and spectrophotometers
analysis.
• Researchgrant No2P04F 033 30 Poland - Main contractor
• SupervisedMasters studentsandconductedundergraduatelaboratories
• Delivered lectures: Microbiology and physiology of food, Toxicology, Ecotoxicology,
Human and animal physiology, Bioindication and biomarkers the environmental
condition
2005, 2007 I delivered biology lectures,PałacMłodzieży, Katowice,Poland
2004-2005 Biology teacher, World-Club, Centrum of Education and Foreign Languages,
Tarnowskie Góry,Poland
ACHIEVEMENTS
2013 Stygar D., Michalczyk K., Dolezych B., Nakonieczny M., Migula P., Zaak M.,
Sawczyn T., Karcz-Socha I., Kukla M., Zwirska-Korczala K., Buldak R; "Digestive
enzymes activity in subsequent generations of Cameraria ohridella larvae
harvested from horse chestnut trees after treatment with imidacloprid." Pestic.
Biochem.Physiol.2013 Jan; 10 (1):5-12.
2012 Zaak M., Migula P., Stygar D., Dolezych B., Michalczyk K.; “Within and
between seasonal changes of detoxifying capabilities of Cameraria ohridella
(Lepidoptera:Gracillariidae) larvae."C.R.Biologies335 (2012) 645-656
2010 Stygar D., Dolezych B., Nakonieczny M., Migula P., Michalczyk K., Zaak M.;
"Digestive enzymes activity in larvae of Cameraria ohridella (Lepidoptera:
Gracillariidae)."CRBiol.2010 Oct; 333(10): 725-35.
2006 VII-th Symposium: Ecologically Friendly Pesticides, Wroclaw University, Bialka
Tatrzanska
 Zaak M., Stygar D.; “Activity of digestive enzymes in Cameraria ohridella
(Lepidoptera:Gracillariidae) larvae underimicacloprid”AbstractVII

3.  Zaak M., Migula P., Sajewicz M., Rzepa J.; „Antioxidative defence in Cameraria
ohridella underimidacloprid”AbstractVII
2005 ECOTOX “AdvencesandTrendsinEcotoxycology”Brno
 Zaak M., Stygar D., Migula P., Dolezych B., „Antioxidant system of Cameraria
ohridella - effectof a host self-defenceandseason.”Bookof abstracts
2003 V-thSymposium:EcologicallyFriendlyPesticides,Wroclaw University,Szczyrk
 Kedziorski A., Nakonieczny M., Stygar D., Zaak M., Rosinski G., „Detoxifying
capabilities of Parnassius apollo sp. Frankenberi larvae in the end part of
development”.Abstarct
OTHER SKILLS
Computer: ProficientuserinMSOffice (Word,Excel,PowerPoint)
Drivinglicense: Full cleandrivinglicence
Nationality: Irish
INTERESTS
• Readingbooks(C. Lackberg,George R.R.Martin)
• TravellingthroughEurope andexplorationof cultures,cuisines,languages and
people (France,Italy, England,Hungary,Czech,Slovakia,Portugal, Austria,
Germany)
• Scrapbooking(making ownpostcards, photoalbum)