Evans Ochieng Ombura is seeking a position that allows him to conduct research to improve lives. He has a BSC in Biochemistry from Kenyatta University with relevant coursework and skills in microbiology, biochemistry, and computer science. His experience includes clinical and research laboratory internships and teaching biology and chemistry. He is proficient in laboratory techniques and safety procedures.
1. 1
CARRICULUM VITAE
PERSONAL DETAILS
NAME: EVANS OCHIENG OMBURA
ID NO: 28633714
NATIONALITY: KENYAN
D.O.B: 18-02-1992
MARITAL STATUS: SINGLE
PHONE NO: 0702874115
EMAIL ADDRESS: omburaevans@gmail.com
HOME ADDRESS: PO BOX.3229. KISUMU
OBJECTIVES
To work for a company that aims to conduct more research that can improve the way of life of
people around the world and wherein I can learn, gain more proficient skills; put my
experience, education, and skills as a biochemist, to ultimate use.
EDUCATION
YEAR INSTITUTION CERTIFICATION
2011-2015 : Kenyatta university BSC (BIOCHEMISTRY)
First Class Honours.
2007-2010 : St Peters Nanga secondary Kenya certificate of secondary
School education (KCSE).Attained a
Mean grade of A-(80pnts)
1999-2006 : kojwach primary school Kenya certificate of primary
Education (KCPE).A-(380mrks)
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RELEVANT COURSE WORK
Good background in biochemistry as well as in supporting subjects such as microbiology, cell
biology, biotechnology chemistry, pharmacology, pharmaceutical chemistry, genetics,
bacteriology, microbial physiology and laboratory techniques.
SPECIAL SKILLS AND LABORATORY TECHNIQUES.
2011-PRESENT
MICROBIOLOGY SKILLS:
Gained skills in aseptic and sterile techniques, optical microscopy, bacterial staining, plating
methods (streak, spread, pour), enumeration and identification of bacteria, use of biological
safety cabinets, media and buffer preparations, diagnostic biochemical tests for enteric
bacteria, use of selective media and antibiotic sensitivity testing of pathogenic bacteria. Basic
mi9croscopy for diagnosis of malaria.
Parasitology and in-vitro culture of malaria parasites including in-vitro assays with cultured
parasites.
BIOCHEMISTRY AND CELL AND MOLECULAR BIOLOGY SKILLS:
Gained skills in DNA extraction and quantification, plasmid DNA preparations, and restriction
enzyme digests.DNA analysis (by conventional and real time PCR and by automated genetic
analyzer).
Routine immunology, hematology and biochemistry techniques for analyzing human clinical
samples including Sample processing for chemistry analysis, biochemical analysis of human
samples by use of SELECTRA E Machine, hematological analysis by ACT 5 Diff hematology auto
analyzer, preparation reading and interpretation of malaria blood film, isolation of peripheral
blood mononuclear cells (PBMCs); counting and cryopreservation of PBMCs.
Enzyme-linked immunosorbent assay (ELISA) for disease diagnosis, protein microarray assays
for antibody profiling and use of Gene Xpert for molecular diagnosis of tuberculosis and
understanding of molecular mechanisms of immunity.
Agarose gel electrophoresis, chromatographic techniques of separation and purification, cell
fractionation by differential centrifugation.
3. 3
Histological skills such as preparations of tissue sections, staining, the Pap smear, and Fine
Needle Aspirations.
Practical aspects of quality control and quality assurance in the clinical and research
laboratories.
CHEMISTRY SKILLS:
Gained skills in solution preparations, titration, extractions, separations, solubility tests, thin
layer chromatography, liquid chromatography.
BIOINFORMATICS SKILLS:
Gained skills in use of NCBI and EMBL databases which include: database searching and
retrieval of sequences, running NCBI BLAST, conserved domain search, multiple sequence
alignment determination using ClustalW,construction of phylogenetic tree, secondary structure
prediction of protein using SIB, primer designing and how to make restriction map.
COMPUTER SKILLS: Programming (use of word, excel, typing) and pubmed.
WORK AND EXPERIENCE
2014: Held a 3 months attachment at Walter Reed Project-Kisumu in their various clinical and
research laboratories which include basic science labs, clinical trial labs, malaria diagnostic
center (MDC) labs and malaria Drug Resistant (MDR) labs.
Highlights of results.
Adopted safety in the laboratory and at the workplace including general laboratory safety,
blood -borne pathogens safety, hazard communication, chemical hygiene and fire safety.
Isolated targeted DNA by PCR (by both conventional and real-time PCR methods), and obtained
pure sample by agarose gel electrophoresis followed by quantification using Nanodrop and
QUBIT kit techniques.
Performed basic microscopy for diagnosis of malaria and cultured malaria parasites in-vitro and
in-vitro assays with cultured parasites.
4. 4
Prepared bacterial culture. Isolated bacteria grown on a culture performed Gram staining for
identification and antibacterial sensitivity testing for pathogenic bacteria.
Carried out ELISA for disease diagnosis, microarray analysis for antibody profiling and
understanding of molecular mechanisms of diseases.
Performed routine immunology, hematology and biochemistry techniques for analyzing human
clinical samples.
Isolated peripheral blood mononuclear cells (PBMCs) from whole blood and counted them
followed by their cryopreservation.
Optimized laboratory instrumentation and stringently followed quality controls, laboratory best
practices and scientific protocols related various biochemical assays.
Through the attachment, I have received hands-on experience on all techniques that are being
applied to answer different research questions at the laboratories. Moreover, I have generated
a good interpersonal relationship through my interaction with my supervisors and colleagues.
This has assisted me to interact confidently with people irrespective of their position. This
exposure has also enabled me to demonstrate aptitude to learn quickly and work with minimum
supervision. In addition, it has also enabled me to gain some additional skills in data collection
and data analysis on the computer and in presentation.
2013: Employed agarose gel electrophoresis, plasmid preparations, restriction enzyme digests,
and tissue sections preparations in biochemistry labs at Kenyatta University. This enabled me to
demonstrate aptitude to learn quickly and work with minimum supervision.
2013: Facilitated laboratory experiments in biology and chemistry for high school students (St
Peters Nanga Secondary School) including the demonstration of proper instrument techniques
resulting in greater understanding of materials by the students and attainment of accurate
results.
2013: Served with the Independent Electoral and Boundary Commission (IEBC) as an election
clerk in the 2013 general election.
5. 5
2012 (September): Participated in a cleanup exercise as a volunteer at Kenyatta national
hospital which enabled me to gain proficiency in working as a cooperative team member.
2012(September semester) Assisted in busy media preparation in microbiology laboratory at
Kenyatta university. Washed and sterilized glassware; prepared and sterilized bacteriological
media, buffers and solutions; poured agar plates; operated autoclaves, drying ovens and PH
meter. This enabled me to demonstrate patience with repeat procedures and team work skills.
2011(March to September): Taught chemistry and biology subjects at St Peters Nanga
secondary school. This enabled me to strengthen my communication skills as an instructor and
also organizational and time management skills.
AWARDS AND WORKSHOPS/SEMINERS ATTENDED
2014: Participated in one day training on Ebola as a disease and case management at Walter
Reed Project-Kisumu organized by the ministry of health.
2010: Participated in a one day forum on leadership in relation to learners’ friendly
environment at kisumu Girls high school and awarded the certificate on the same.
2007: Attended one week training on peer education at GOT OWAK, kisumu and awarded a
certificate on the same.
2015: participated and received a three months training course on leadership and mentorship
at Kenyatta University. The training imparted me with invaluable leadership and management
skills in relation to development, mentorship and professionalism.
ACTIVITIES AND INTERESTS
Reading (science fiction, mysteries)
Football, volleyball and solving and compiling crossword puzzles.
Traveling.
Listening to music.
6. 6
REFEREES
Madam Anne Nyamamba
Principal; St Peters Nanga secondary school.
PO BOX 3229.kisumu
PHONE NO: 0733931405.
Dr Daniel Okun
Department of biochemistry and biotechnology,
Kenyatta University,
PO BOX43844-00100, Nairobi Kenya
PHONE NO: 0725991515.
Dr John Waitumbi,
Director Walter Reed Project-Kisumu.
PO BOX 54, 40100 KISUMU
PHONE NO 0733616548.