This presentation basically talks about the potential possible applications of microbes in petroleum industry.

1. MICROBES
Dhaval Patel
15BPE076

2. PRESENTATION
 1. Microbes
 2. How it works
 3. Uses/applications

3. MICROBES
 3.5 billion years ago, micro organisms have evolved
which can use carbon compounds as food.
 And HC are also essentially, chains of hydrogen and
CARBON.
 Many bacterias can digest crude oil.

4. OIL EATERS

5. OIL EATERS
 Oil eater microbes (OEM) are very useful in various
steps of different petroleum activities.
 Eg. Exploration, EOR, well to well correlations ,for oil
spill cleaning , etc.

6. HOW IT WORKS
 Oil eaters bacterias or microbes EAT the oil.
 They converts oil into surfactants.
 And releases the water , carboxylic acids and carbon
dioxide.(not harmful to ocean's plants and animals)

7. USES/APPLICATIONS
 1. In enhanced oil recoveries
 2. To clean the oil spills
 3. In modern oil exploration works.

8. EOR
 Microbes digests the oil and release the surfactants, (and
reduces viscocity of oil and IFT between oil and
brine.(microbial E.O.R.))

9. OIL SPILLS
 Oil eaters Microbes can eat almost all oil spilled.
 Special kind of adsorbers are prepared by immersing
adsorber material in OEM solution before using it for oil
clean ups.
 Adsorber material adsorb all spilled oil and OEM convert
that adsorbed oil into non toxic surfactants and release them.

10. EXAMPLE
Deep water horizon oil spill, mexico.
After spill,They released adsorber substance in spilled oil(
called ,SOT which has oil eaters and oil adsorbers in it.)
So, by putting SOT in spilled oil,oil adsorbers adsorb oil
and get heavier than initial one,and settled down in sea.
Following action were taken by oil eaters.(so,that marine
life is not affected by oil.)

12. BIOREMEDIATION
 Bioremediation is use of naturally occurring or
introduced micro organisms or other forms of life to
consume or break down environment pollution in order
to clean up the polluted area
 (Collins , english dictionary 2015)

13.  2 ways of bioremediation
 Add nutrients to already exiting OEM (in their own
environment) for their further growth
 Or
 Introduce OEM from outside and feed nutrients to
them.

14. EXPLORATION
 Oil eaters microbes are generally found nearby oil
places.(nearby petrol pumps also)
 So,presence of oil eaters bacterias in core, or formation
indicates that there might be oil in nearby areas.

15. DATA FROM ARGENTINA
BASIN

16. EXPLORATION
 Oil eaters bacterias and oil quantity inside plants tissues
indicates that, there were oil spill happened in that or
nearby area.
 (eg. Mexico's after oil spill study )

17.  In soil nearby seabed, certain type of bacteria can
indicate the presence of hydrocarbons.

18. ADVANTAGES
 Low cost
 No need of complex technology.
 Do not involve any type of chemical compounds
injection.
 It is mostly non hazardous process for environment.

19. RESEARCH
 Research conducted by Casey Hubert at the University of
Calgary,canada.

21. WHAT TYPES OF BACTERIA WE ARE LOOKING AND
WHY ?

22. We interested in three groups of bacteria.
1.Bacteria that eat hydrocarbons and breathe with oxygen.
(where oxygenated water comes into contact with the
hydrocarbons.(aerobic environment))
2.Bacteria that eat hydrocarbons but breathe with alternative
electron acceptors like sulphate.( typically found a little bit
deeper in the sediment where there is no longer any
oxygen.(anaerobic environment))
Thirdly, we are interested in a group of bacteria that can only
grow at specific high temperatures – “thermophiles”.

23.  Can it directly save offshore operators money ?
 If microbiological assays can help companies avoid
drilling a dry hole – then based on the cost per hole, there
are millions to billions in cost savings.

24. REFERENCE
 Casey Hubert interview ,the University of Calgary,canada.
 Research papers (microbes fact sheets ).
 Taylorscinecegeeks(
http://taylorsciencegeeks.weebly.com/blog/oil-eating-
bacteria-new-technology-using-microorganisms).
 Oil spill and their remedies.
 Microbial application in EOR process.
 Collins , english dictionary (2015).
 Gulf of mexico,oil spill.
 Youtube.
 Google.
 Wikipedia.

25. આભાર
Thank you
शुक्रिया